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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6730R-3 |
| Symbol |
Cyp4d21 |
Full Name |
Cyp4d21 |
| CG No |
CG6730 |
Old CG No |
CG6730 |
| Synonyms |
sxe1, CG6730, 4d21, Cyp4d21 |
| Accession No (Link to NCBI) |
NM_135285.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTCTGCGTGATTTTCTTGGCGA |
| Primer Seq. 3' |
aatctagaggtaccTTATCAGCGGCTTGTAGGTGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TCTGCGTGAT TTTCTTGGCG ATGGACTTCT TTTAAGCACG GGAAATAAGT GGACTTCTCG
0061 CAGAAAGGTT TCTCGCACCT GCTTTTCACT TCAAATGCCT TGAAAACTTC GTCGAGATAA
0121 TGGATAGAAA TAGTGGAATT ATGGTGGAGA AATTGAAAAA CTATGCTGAT GGCAAAACGT
0181 GCGTGGACCT ATTCAAGTTT GTTTCTCTGG AAGCCCTGGA TGTGACCACA GAGACCGCCA
0241 TGGGTGTCCA GGTGAATGCT CAGAACGAAC CAAATTTCCC CTATACAAAA GCTCTTAAAA
0301 GTGTGGTTTA TATCGAGTCC AAACGATTGG CAAGTGTCTC CATGCGGTAT AATTGGCTTT
0361 TCCCGCTGGC GGCTCCTTTG GTTTATCGCC GTTTGCAAAA AGACATAGCT ATAATGCAGG
0421 ACTTTACTGA CAAGGTCATT CGCGAACGAC GAACGATTCT GGAGCGGGCC AGGGCGGATG
0481 GCACCTACAA GCCGCTGATA A |
| in silico PCR Fragment |
0001 TCTGCGTGAT TTTCTTGGCG ATGGACTTCT TTTAAGCACG GGAAATAAGT GGACTTCTCG
0061 CAGAAAGG-T TCTCGCACCT GCTTTTCACT TCAAATGCCT TGAAAACTTC GTCGAGATAA
0121 TGGATAGAAA TAGTGGAATT ATGGTGGAGA AATTGAAAAA CTATGCTGAT GGCAAAACGT
0181 GCGTGGACCT ATTCAAGTTT GTTTCTCTGG AAGCCCTGGA TGTGACCACA GAGACCGCCA
0241 TGGGTGTCCA GGTGAATGCT CAGAACGAAC CAAATTTCCC CTATACAAAA GCTCTTAAAA
0301 GTGTGGTTTA TATCGAGTCC AAACGATTGG CAAGTGTCTC GATGCGGTAT AATTGGCTTT
0361 TCCCGCTGGC GGCTCCTTTG GTTTATCGCC GTTTGCAAAA AGACATAGCT ATAATGCAGG
0421 ACTTTACTGA CAAGGTCATT CGCGAACGAC GAGCGATTCT GGAGCGGGCC AGGGCGGATG
0481 GCACCTACAA GCCGCTGATA A |
| Assemble Data |
6730R-3.IR_full 1 TCTGCGTGATTTTCTTGGCGATGGACTTCTTTTAAGCACGGGAAATAAGTGGACTTCTCG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6730R-3.in silico 1 TCTGCGTGATTTTCTTGGCGATGGACTTCTTTTAAGCACGGGAAATAAGTGGACTTCTCG 60
6730R-3.IR_full 61 CAGAAAGGTTTCTCGCACCTGCTTTTCACTTCAAATGCCTTGAAAACTTCGTCGAGATAA 120
|||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
6730R-3.in silico 61 CAGAAAGG-TTCTCGCACCTGCTTTTCACTTCAAATGCCTTGAAAACTTCGTCGAGATAA 120
6730R-3.IR_full 121 TGGATAGAAATAGTGGAATTATGGTGGAGAAATTGAAAAACTATGCTGATGGCAAAACGT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6730R-3.in silico 121 TGGATAGAAATAGTGGAATTATGGTGGAGAAATTGAAAAACTATGCTGATGGCAAAACGT 180
6730R-3.IR_full 181 GCGTGGACCTATTCAAGTTTGTTTCTCTGGAAGCCCTGGATGTGACCACAGAGACCGCCA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6730R-3.in silico 181 GCGTGGACCTATTCAAGTTTGTTTCTCTGGAAGCCCTGGATGTGACCACAGAGACCGCCA 240
6730R-3.IR_full 241 TGGGTGTCCAGGTGAATGCTCAGAACGAACCAAATTTCCCCTATACAAAAGCTCTTAAAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6730R-3.in silico 241 TGGGTGTCCAGGTGAATGCTCAGAACGAACCAAATTTCCCCTATACAAAAGCTCTTAAAA 300
6730R-3.IR_full 301 GTGTGGTTTATATCGAGTCCAAACGATTGGCAAGTGTCTCCATGCGGTATAATTGGCTTT 360
|||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
6730R-3.in silico 301 GTGTGGTTTATATCGAGTCCAAACGATTGGCAAGTGTCTCGATGCGGTATAATTGGCTTT 360
6730R-3.IR_full 361 TCCCGCTGGCGGCTCCTTTGGTTTATCGCCGTTTGCAAAAAGACATAGCTATAATGCAGG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6730R-3.in silico 361 TCCCGCTGGCGGCTCCTTTGGTTTATCGCCGTTTGCAAAAAGACATAGCTATAATGCAGG 420
6730R-3.IR_full 421 ACTTTACTGACAAGGTCATTCGCGAACGACGAACGATTCTGGAGCGGGCCAGGGCGGATG 480
|||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
6730R-3.in silico 421 ACTTTACTGACAAGGTCATTCGCGAACGACGAGCGATTCTGGAGCGGGCCAGGGCGGATG 480
6730R-3.IR_full 481 GCACCTACAAGCCGCTGATAA 501
|||||||||||||||||||||
6730R-3.in silico 481 GCACCTACAAGCCGCTGATAA 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_135285.2 |
CG6730-RA (Cyp4d21), mRNA |
| 0 |
0 |
12 |
30 |
38 |
NM_139466.3 |
CG16761-RA (Cyp4d20), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_058023.4 |
CG4900-RA (Irp-1A), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_141204.3 |
CG1084-RA (Cont), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_079549.2 |
CG7850-RA (puc), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_136783.2 |
CG30020-RA (CG30020), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_079823.2 |
CG1395-RA (stg), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_142649.2 |
CG4000-RA (CG4000), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136839.2 |
CG30023-RA (sprt), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_141854.2 |
CG14722-RA (CG14722), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137416.2 |
CG12298-RA (sub), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_206342.1 |
CG33265-RA (CG33265), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_134591.2 |
CG1501-RA (unc), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_001032020.1 |
CG7217-RB, transcript variant B (CG7217), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_142422.3 |
CG7217-RA, transcript variant A (CG7217), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_142423.2 |
CG7215-RA, transcript variant A (CG7215), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_140268.1 |
CG7257-RA (CG7257), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_141228.1 |
CG12001-RA (CG12001), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_080026.2 |
CG10847-RB, transcript variant B (enc), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_206270.1 |
CG10847-RA, transcript variant A (enc), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_142503.2 |
CG14291-RA (CG14291), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_165374.1 |
CG8669-RB, transcript variant B (crc), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_080158.2 |
CG8669-RA, transcript variant A (crc), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_169549.1 |
CG9351-RC, transcript variant C (flfl), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_142047.1 |
CG9351-RA, transcript variant A (flfl), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_169548.1 |
CG9351-RB, transcript variant B (flfl), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_078867.2 |
CG5526-RA (Dhc36C), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_080227.1 |
CG11390-RA (PebIII), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_001042883.1 |
CG18405-RC, transcript variant C (Sema-1a), mRNA |
| 0 |
0 |
0 |
3 |
2 |
NM_133016.2 |
CG6398-RA (CG6398), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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