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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 6726R-3 
 Symbol CG6726  Full Name CG6726 
 CG No CG6726  Old CG No CG6726 
 Synonyms CG6726 
 Accession No (Link to NCBI) NM_142863.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGGGCACCCAATATCTGGGAG 
 Primer Seq. 3' 
aatctagaggtaccGATATCCACGGTGACGGCAAT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TGGGCACCCA ATATCTGGGA GCTGTgNNNG CCCTCAAGGC CAGTgGNTAT CAACCCAAGA 
0061 GGACGATCTA TCTCACTTAT GTTCCGGACG AGGAGGTTGG CGGCCACCNG GGcaTNCGGG 
0121 NNCTGGTAAA GAGCGACTAC TTCAAGAAGC TCAATGTGGG ATTCAGTTTC GACGAGGGAA 
0181 TCTCTAGTGC GGATNAAANC TANGCTCTGT ACTANGCTGA ANGGACTCTT TGGCATCTTC 
0241 GTTTCAAGAT CAGCGGAACT GCTGGTCACG GATCACTGTT GCTCCCGAAT ACTGCTGGCG 
0301 AGAAGCTGAA CTATGTGGTA GGCAAAATGA TGGAGTTCCG CAAATCGCAA GTGCAAAAAC 
0361 TAGCTGACGA CTCATCCCTC GAAATTGGTG ATGTGACCAC TGTGAATCTC ACCCAACTGA 
0421 GGGGCGGAGT CCAGAGCAAC GTNGTTCCCC CATTGCTGGA AGCTGTATTT GATATCCGCA 
0481 TTGCCGTCAC CGTGGATATC  
 in silico PCR Fragment 
0001 TGGGCACCCA ATATCTGGGA GCTGTGCGTG CCCTCAAGGC CAGTGGCTAT CAACCCAAGA 
0061 GGACGATCTA TCTCACTTAT GTTCCGGACG AGGAGGTTGG CGGCCACCTG GGCATGCGGG 
0121 AGCTGGTAAA GAGCGACTAC TTCAAGAAGC TCAATGTGGG ATTCAGTTTC GACGAGGGAA 
0181 TCTCTAGTGC GGATGAAACC TACGCTCTGT ACTACGCTGA ACGGACTCTT TGGCATCTTC 
0241 GTTTCAAGAT CAGCGGAACT GCTGGTCACG GATCACTGTT GCTCCCGAAT ACTGCTGGCG 
0301 AGAAGCTGAA CTATGTGGTA GGCAAAATGA TGGAGTTCCG CAAATCGCAA GTGCAAAAAC 
0361 TAGCTGACGA CTCATCCCTC GAAATTGGTG ATGTGACCAC TGTGAATCTC ACCCAACTGA 
0421 GGGGCGGAGT CCAGAGCAAC GTGGTTCCCC CATTGCTGGA AGCTGTATTT GATATCCGCA 
0481 TTGCCGTCAC CGTGGATATC  
 Assemble Data 

6726R-3.IR_full       1   TGGGCACCCAATATCTGGGAGCTGTGNNNGCCCTCAAGGCCAGTGGNTATCAACCCAAGA 60
                          ||||||||||||||||||||||||||   ||||||||||||||||| |||||||||||||
6726R-3.in silico     1   TGGGCACCCAATATCTGGGAGCTGTGCGTGCCCTCAAGGCCAGTGGCTATCAACCCAAGA 60


6726R-3.IR_full       61  GGACGATCTATCTCACTTATGTTCCGGACGAGGAGGTTGGCGGCCACCNGGGCATNCGGG 120
                          |||||||||||||||||||||||||||||||||||||||||||||||| |||||| ||||
6726R-3.in silico     61  GGACGATCTATCTCACTTATGTTCCGGACGAGGAGGTTGGCGGCCACCTGGGCATGCGGG 120


6726R-3.IR_full        N121 CTGGTAAAGAGCGACTACTTCAAGAAGCTCAATGTGGGATTCAGTTTCGACGAGGGAA 178
                            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6726R-3.in silico      A121 CTGGTAAAGAGCGACTACTTCAAGAAGCTCAATGTGGGATTCAGTTTCGACGAGGGAA 178


6726R-3.IR_full       181 TCTCTAGTGCGGATNAAANCTANGCTCTGTACTANGCTGAANGGACTCTTTGGCATCTTC 238
                          |||||||||||||| ||| ||| ||||||||||| |||||| ||||||||||||||||||
6726R-3.in silico     181 TCTCTAGTGCGGATGAAACCTACGCTCTGTACTACGCTGAACGGACTCTTTGGCATCTTC 238


6726R-3.IR_full       241 GTTTCAAGATCAGCGGAACTGCTGGTCACGGATCACTGTTGCTCCCGAATACTGCTGGCG 298
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6726R-3.in silico     241 GTTTCAAGATCAGCGGAACTGCTGGTCACGGATCACTGTTGCTCCCGAATACTGCTGGCG 298


6726R-3.IR_full       301 AGAAGCTGAACTATGTGGTAGGCAAAATGATGGAGTTCCGCAAATCGCAAGTGCAAAAAC 358
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6726R-3.in silico     301 AGAAGCTGAACTATGTGGTAGGCAAAATGATGGAGTTCCGCAAATCGCAAGTGCAAAAAC 358


6726R-3.IR_full       361 TAGCTGACGACTCATCCCTCGAAATTGGTGATGTGACCACTGTGAATCTCACCCAACTGA 418
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6726R-3.in silico     361 TAGCTGACGACTCATCCCTCGAAATTGGTGATGTGACCACTGTGAATCTCACCCAACTGA 418


6726R-3.IR_full       421 GGGGCGGAGTCCAGAGCAACGTNGTTCCCCCATTGCTGGAAGCTGTATTTGATATCCGCA 478
                          |||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
6726R-3.in silico     421 GGGGCGGAGTCCAGAGCAACGTGGTTCCCCCATTGCTGGAAGCTGTATTTGATATCCGCA 478


6726R-3.IR_full       481 TTGCCGTCACCGTGGATATC 498
                          ||||||||||||||||||||
6726R-3.in silico     481 TTGCCGTCACCGTGGATATC 498



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_170048.1  CG6726-RB, transcript variant B (CG6726), mRNA 
100   482  0  0  2  NM_142863.1  CG6726-RA, transcript variant A (CG6726), mRNA 
0.2   1  4  4  2  NM_140903.1  CG7809-RA (Grasp65), mRNA 
0   0  18  12  24  NM_142866.1  CG6733-RA (CG6733), mRNA 
0   0  8  33  44  NM_142867.1  CG6738-RA (CG6738), mRNA 
0   0  4  21  37  NM_142864.1  CG17110-RA (CG17110), mRNA 
0   0  2  25  62  NM_142865.2  CG17109-RA (CG17109), mRNA 
0   0  2  2  2  NM_140808.1  CG6852-RA (CG6852), mRNA 
0   0  1  8  9  NM_001043114.1  CG33484-RD, transcript variant D (zormin), mRNA 
0   0  1  5  3  NM_132370.1  CG2989-RA (CG2989), mRNA 
0   0  1  4  2  NM_134850.2  CG3210-RA (Drp1), mRNA 
0   0  1  3  3  NM_143343.2  CG4963-RA (CG4963), mRNA 
0   0  1  3  2  NM_079510.2  CG10612-RA (Or83a), mRNA 
0   0  0  14  47  NM_141747.2  CG6465-RA (CG6465), mRNA 
0   0  0  10  4  NM_136443.2  CG1620-RA (CG1620), mRNA 
0   0  0  6  4  NM_139950.1  CG13670-RA (CG13670), mRNA 
0   0  0  6  3  NM_169206.1  CG9656-RA (grn), mRNA 
0   0  0  5  3  NM_136677.1  CG1663-RA (CG1663), mRNA 
0   0  0  4  7  NM_206228.1  CG18214-RF, transcript variant F (trio), mRNA 
0   0  0  4  7  NM_167849.1  CG18214-RB, transcript variant B (trio), mRNA 
0   0  0  4  6  NM_167847.1  CG18214-RC, transcript variant C (trio), mRNA 
0   0  0  4  6  NM_143703.2  CG18214-RA, transcript variant A (trio), mRNA 
0   0  0  4  6  NM_167848.1  CG18214-RD, transcript variant D (trio), mRNA 
0   0  0  4  5  NM_168468.1  CG32091-RB (CG32091), mRNA 
0   0  0  4  4  NM_176778.1  CG14616-RD, transcript variant D (l(1)G0196), mRNA 
0   0  0  4  4  NM_176779.1  CG14616-RA, transcript variant A (l(1)G0196), mRNA 
0   0  0  4  4  NM_176780.1  CG14616-RE, transcript variant E (l(1)G0196), mRNA 
0   0  0  4  4  NM_176777.1  CG14616-RC, transcript variant C (l(1)G0196), mRNA 
0   0  0  4  2  NM_079518.2  CG11422-RA (Os-E), mRNA 
0   0  0  3  5  NM_136991.2  CG3915-RB, transcript variant B (Drl-2), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+ 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
+ 
DC
 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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