NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 6716R-3

Symbol prd Full Name paired

CG No CG6716 Old CG No CG6716

Synonyms Prd, pr, CG6716, prd

Accession No (Link to NCBI) NM_078832.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAACGGTCGTCCTTTGCCCAAC

Primer Seq. 3'

aatctagaggtaccCCCGGCTCACTTTCACAGTCA

Predicted Fragment Size

IR fragment full Seq

0001 AACGGTCGTC CTTTGCCCAA CAATATTCGT CTTAAAATCG TCGAGATGGC CGCCGATGGC 
0061 ATTCGGCCCT GTGTGATCTC CAGACAGCTA CGTGTGTCCC ATGGCTGCGT ATCGAAGATC 
0121 CTGAATCGCT ACCAGGAGAC TGGCTCCATT AGACCAGGCG TAATTGGTGG CTCCAAGCCG 
0181 AGGATANGCC ACGCCCGAAA TCGAAAACCG AATTGAGGAG TACAAGCGCA GTAGCCCGGG 
0241 CATGTTCTCG TGGGAGATCA GGGAGAAGCT GATCCGCGAG GGAGTCTGCG ACAGGAGCAC 
0301 AGCACCATCT GTGTCCGCCA TATCGCGCCT GGTGCGCGGC CGNAGATGCT CCATTGGACA 
0361 ATGATATGTC TTCTGCCTCT GGATCTCCGG CGGGTGATGG CACCAAAGCA TCGAGTTCCT 
0421 GCGGCTCCGA TGTCTCAGGT GGCCATCACA ACAACGGCAA GCCCTCCGAT GAGGACATCT 
0481 CTGACTGTGA AAGTGAGCCG GG

in silico PCR Fragment

0001 AACGGTCGTC CTTTGCCCAA CAATATTCGT CTTAAAATCG TCGAGATGGC CGCCGATGGC 
0061 ATTCGGCCCT GTGTGATCTC CAGACAGCTA CGTGTGTCCC ATGGCTGCGT ATCGAAGATC 
0121 CTGAATCGCT ACCAGGAGAC TGGCTCCATT AGACCAGGCG TAATTGGTGG CTCCAAGCCG 
0181 AGGATA-GCC ACGCCCGAAA TCGAGAACCG AATTGAGGAG TACAAGCGCA GTAGCCCGGG 
0241 CATGTTCTCG TGGGAGATCA GGGAGAAGCT GATCCGCGAG GGTGTCTGCG ACAGGAGCAC 
0301 AGCACCATCT GTGTCCGCCA TATCGCGCCT GGTGCGCGGC CG-AGATGCT CCATTGGACA 
0361 ATGATATGTC TTCTGCCTCT GGATCTCCGG CGGGTGATGG CACCAAAGCA TCGAGTTCCT 
0421 GCGGCTCCGA TGTCTCAGGT GGCCATCACA ACAACGGCAA GCCCTCCGAT GAGGACATCT 
0481 CTGACTGTGA AAGTGAGCCG GG

Assemble Data


6716R-3.IR_full       1   AACGGTCGTCCTTTGCCCAACAATATTCGTCTTAAAATCGTCGAGATGGCCGCCGATGGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6716R-3.in silico     1   AACGGTCGTCCTTTGCCCAACAATATTCGTCTTAAAATCGTCGAGATGGCCGCCGATGGC 60


6716R-3.IR_full       61  ATTCGGCCCTGTGTGATCTCCAGACAGCTACGTGTGTCCCATGGCTGCGTATCGAAGATC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6716R-3.in silico     61  ATTCGGCCCTGTGTGATCTCCAGACAGCTACGTGTGTCCCATGGCTGCGTATCGAAGATC 120


6716R-3.IR_full       121 CTGAATCGCTACCAGGAGACTGGCTCCATTAGACCAGGCGTAATTGGTGGCTCCAAGCCG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6716R-3.in silico     121 CTGAATCGCTACCAGGAGACTGGCTCCATTAGACCAGGCGTAATTGGTGGCTCCAAGCCG 180


6716R-3.IR_full       181 AGGATANGCCACGCCCGAAATCGAAAACCGAATTGAGGAGTACAAGCGCAGTAGCCCGGG 240
                          |||||| ||||||||||||||||| |||||||||||||||||||||||||||||||||||
6716R-3.in silico     181 AGGATA-GCCACGCCCGAAATCGAGAACCGAATTGAGGAGTACAAGCGCAGTAGCCCGGG 240


6716R-3.IR_full       241 CATGTTCTCGTGGGAGATCAGGGAGAAGCTGATCCGCGAGGGAGTCTGCGACAGGAGCAC 300
                          |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
6716R-3.in silico     241 CATGTTCTCGTGGGAGATCAGGGAGAAGCTGATCCGCGAGGGTGTCTGCGACAGGAGCAC 300


6716R-3.IR_full       301 AGCACCATCTGTGTCCGCCATATCGCGCCTGGTGCGCGGCCGNAGATGCTCCATTGGACA 360
                          |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
6716R-3.in silico     301 AGCACCATCTGTGTCCGCCATATCGCGCCTGGTGCGCGGCCG-AGATGCTCCATTGGACA 360


6716R-3.IR_full       361 ATGATATGTCTTCTGCCTCTGGATCTCCGGCGGGTGATGGCACCAAAGCATCGAGTTCCT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6716R-3.in silico     361 ATGATATGTCTTCTGCCTCTGGATCTCCGGCGGGTGATGGCACCAAAGCATCGAGTTCCT 420


6716R-3.IR_full       421 GCGGCTCCGATGTCTCAGGTGGCCATCACAACAACGGCAAGCCCTCCGATGAGGACATCT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6716R-3.in silico     421 GCGGCTCCGATGTCTCAGGTGGCCATCACAACAACGGCAAGCCCTCCGATGAGGACATCT 480


6716R-3.IR_full       481 CTGACTGTGAAAGTGAGCCGGG 502
                          ||||||||||||||||||||||
6716R-3.in silico     481 CTGACTGTGAAAGTGAGCCGGG 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_164990.2	CG6716-RB, transcript variant B (prd), mRNA
100	482	0	0	0	NM_078832.2	CG6716-RA, transcript variant A (prd), mRNA
0.2	1	7	11	34	NM_079139.2	CG3388-RA (gsb), mRNA
0	0	7	19	42	NM_079138.1	CG2692-RA (gsb-n), mRNA
0	0	4	2	8	NM_143409.1	CG11873-RA (CG11873), mRNA
0	0	3	6	16	NM_079317.2	CG10704-RA (toe), mRNA
0	0	3	3	2	NM_139759.1	CG6592-RA (CG6592), mRNA
0	0	2	3	4	NM_167943.1	CG32304-RA (CG32304), mRNA
0	0	2	2	8	NM_169609.1	CG31304-RA (CG31304), mRNA
0	0	2	2	4	NM_132540.1	CG2467-RA, transcript variant A (CG2467), mRNA
0	0	2	2	4	NM_167313.1	CG2467-RB, transcript variant B (CG2467), mRNA
0	0	1	9	8	NM_079318.2	CG10488-RA, transcript variant A (eyg), mRNA
0	0	1	9	8	NM_001014582.1	CG10488-RB, transcript variant B (eyg), mRNA
0	0	1	3	3	NM_144457.2	CG18530-RA (CG18530), mRNA
0	0	1	3	2	NM_141602.2	CG11982-RA (CG11982), mRNA
0	0	1	3	2	NM_139758.1	CG10475-RA (Jon65Ai), mRNA
0	0	1	3	2	NM_079869.2	CG1528-RA, transcript variant A (gammaCop), mRNA
0	0	1	3	2	NM_170553.1	CG1528-RB, transcript variant B (gammaCop), mRNA
0	0	1	2	4	NM_140321.1	CG10660-RA (CG10660), mRNA
0	0	1	2	3	NM_001042910.1	CG11628-RB, transcript variant B (Grp1), mRNA
0	0	1	2	2	NM_079398.2	CG7930-RA, transcript variant A (TpnC73F), mRNA
0	0	1	2	2	NM_168715.1	CG7930-RB, transcript variant B (TpnC73F), mRNA
0	0	0	8	16	NM_057338.2	CG8246-RA (Poxn), mRNA
0	0	0	7	6	NM_079808.2	CG5658-RA (Klp98A), mRNA
0	0	0	6	2	NM_167541.1	CG32569-RA (CG32569), mRNA
0	0	0	5	3	NM_141000.1	CG3698-RA (CG3698), mRNA
0	0	0	4	20	NM_001043222.1	CG9610-RC (Poxm), mRNA
0	0	0	4	7	NM_143745.2	CG18389-RA (Eip93F), mRNA
0	0	0	4	6	NM_078869.4	CG15154-RB, transcript variant B (Socs36E), mRNA
0	0	0	4	6	NM_165243.1	CG15154-RA, transcript variant A (Socs36E), mRNA

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