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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 6705R-2 
 Symbol tsl  Full Name torso-like 
 CG No CG6705  Old CG No CG6705 
 Synonyms fs(3)00617, CG6705, tsl 
 Accession No (Link to NCBI) NM_079716.2 
 Inserted Chr. X 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354 [ PubMed ID = 29764959 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCTGGCCTTTTCTGGCTGCTG 
 Primer Seq. 3' 
aatctagaggtaccGGTTGGTGCACCGGAATCTCA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CCTGGCCTTT TCTGGCTGCT GACCCTGGCG CTCCTGGCGG ATGGAGGTCG TCGCGAGTCC 
0061 CAGCTCCGGA TCGGCAAGGC CATCAATATA TTCCTGCGCT ATGGCTACCT GGGAATCTCC 
0121 ATGCGTGTGA TACCGCTCAA TGACAACTCG GAGCCGGATC GCTGGGTGTT TAAGGAGCCC 
0181 ACCAAAAACA TCTATAGGAA TCTGAGTGGC CTGGCGGAGA GTCATGAAGA CACCACGCCC 
0241 GGAATCTTTC ATGGAGACTT TCACATGGAG TTCTGCGAGA ATCGGAGGCA ANCTGTTCCA 
0301 GGCCTACTTC CGTGACTTTT CCATTGAGCG AATGGACAAA CCGTGGGAGG CATTTACTGG 
0361 CGGATGGTTT CCGGATAATG CGGCCAAGAA GCTGGGCATC AATACATCTT TCATCCAAGG 
0421 CGATTACTCG TATGTTTTGG TGAGGGTGGT GCGCTTCAGG GAAACgGGGN GTCTTAACGC 
0481 TGAGATTCCG GTGCACCAAC C 
 in silico PCR Fragment 
0001 CCTGGCCTTT TCTGGCTGCT GACCCTGGCG CTCCTGGCGG ATGGAGGTCG TCGCGAGTCC 
0061 CAGCTCCGGA TCGGCAAGGC CATCAATATA TTCCTGCGCT ATGGCTACCT GGGAATCTCC 
0121 ATGCGTGTGA TACCGCTCAA TGACAACTCG GAGCCGGATC GCTGGGTGTT TAAGGAGCCC 
0181 ACCAAAAACA TCTATAGGAA TCTGAGTGGC CTGGCGGAGA GTCATGAAGA CACCACGCCC 
0241 GGAATCTTTC ATGGAGACTT TCACATGGAG TTCTGCGAGA ATCGGAGGCA A-CTGTTCCA 
0301 GGCCTACTTC CGTGACTTTT CCATTGAGCG AATGGACAAA CCGTGGGAGG CATTTACTGG 
0361 CGGATGGTTT CCGGATAATG CGGCCAAGAA GCTGGGCATC AATACATCTT TCATCCAAGG 
0421 CGATTACTCG TATGTTTTGG TGAGGGTGGT GCGCTTCAGG GAAACGGGGC GTCTTAACGC 
0481 TGAGATTCCG GTGCACCAAC C 
 Assemble Data 

6705R-2.IR_full       1   CCTGGCCTTTTCTGGCTGCTGACCCTGGCGCTCCTGGCGGATGGAGGTCGTCGCGAGTCC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6705R-2.in silico     1   CCTGGCCTTTTCTGGCTGCTGACCCTGGCGCTCCTGGCGGATGGAGGTCGTCGCGAGTCC 60


6705R-2.IR_full       61  CAGCTCCGGATCGGCAAGGCCATCAATATATTCCTGCGCTATGGCTACCTGGGAATCTCC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6705R-2.in silico     61  CAGCTCCGGATCGGCAAGGCCATCAATATATTCCTGCGCTATGGCTACCTGGGAATCTCC 120


6705R-2.IR_full       121 ATGCGTGTGATACCGCTCAATGACAACTCGGAGCCGGATCGCTGGGTGTTTAAGGAGCCC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6705R-2.in silico     121 ATGCGTGTGATACCGCTCAATGACAACTCGGAGCCGGATCGCTGGGTGTTTAAGGAGCCC 180


6705R-2.IR_full       181 ACCAAAAACATCTATAGGAATCTGAGTGGCCTGGCGGAGAGTCATGAAGACACCACGCCC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6705R-2.in silico     181 ACCAAAAACATCTATAGGAATCTGAGTGGCCTGGCGGAGAGTCATGAAGACACCACGCCC 240


6705R-2.IR_full       241 GGAATCTTTCATGGAGACTTTCACATGGAGTTCTGCGAGAATCGGAGGCAANCTGTTCCA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
6705R-2.in silico     241 GGAATCTTTCATGGAGACTTTCACATGGAGTTCTGCGAGAATCGGAGGCAA-CTGTTCCA 300


6705R-2.IR_full       301 GGCCTACTTCCGTGACTTTTCCATTGAGCGAATGGACAAACCGTGGGAGGCATTTACTGG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6705R-2.in silico     301 GGCCTACTTCCGTGACTTTTCCATTGAGCGAATGGACAAACCGTGGGAGGCATTTACTGG 360


6705R-2.IR_full       361 CGGATGGTTTCCGGATAATGCGGCCAAGAAGCTGGGCATCAATACATCTTTCATCCAAGG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6705R-2.in silico     361 CGGATGGTTTCCGGATAATGCGGCCAAGAAGCTGGGCATCAATACATCTTTCATCCAAGG 420


6705R-2.IR_full       421 CGATTACTCGTATGTTTTGGTGAGGGTGGTGCGCTTCAGGGAAACGGGGNGTCTTAACGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
6705R-2.in silico     421 CGATTACTCGTATGTTTTGGTGAGGGTGGTGCGCTTCAGGGAAACGGGGCGTCTTAACGC 480


6705R-2.IR_full       481 TGAGATTCCGGTGCACCAACC 501
                          |||||||||||||||||||||
6705R-2.in silico     481 TGAGATTCCGGTGCACCAACC 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_079716.2  CG6705-RA, transcript variant A (tsl), mRNA 
100   482  0  0  0  NM_169969.1  CG6705-RB, transcript variant B (tsl), mRNA 
0.2   1  3  2  4  NM_137618.1  CG9864-RA (CG9864), mRNA 
0   0  4  3  4  NM_134486.1  CG14221-RA (CG14221), mRNA 
0   0  3  4  4  NM_079824.2  CG18741-RB, transcript variant B (DopR2), mRNA 
0   0  3  4  4  NM_170420.1  CG18741-RA, transcript variant A (DopR2), mRNA 
0   0  2  3  3  NM_132304.2  CG9060-RA (Zpr1), mRNA 
0   0  2  3  2  NM_134676.1  CG4213-RA (CG4213), mRNA 
0   0  2  2  4  NM_132170.1  CG11369-RA (CG11369), mRNA 
0   0  2  2  4  NM_058119.3  CG18783-RA, transcript variant A (Kr-h1), mRNA 
0   0  2  2  4  NM_058118.3  CG18783-RB, transcript variant B (Kr-h1), mRNA 
0   0  1  5  2  NM_164444.1  CG31663-RA (CG31663), mRNA 
0   0  1  4  4  NM_057365.3  CG3182-RA, transcript variant A (sei), mRNA 
0   0  1  4  4  NM_166653.1  CG3182-RB, transcript variant B (sei), mRNA 
0   0  1  4  3  NM_164678.1  CG12393-RB, transcript variant B (CG12393), mRNA 
0   0  1  4  3  NM_135140.2  CG12393-RA, transcript variant A (CG12393), mRNA 
0   0  1  4  3  NM_136037.2  CG10275-RA (CG10275), mRNA 
0   0  1  4  2  NM_140648.1  CG4169-RA (CG4169), mRNA 
0   0  1  3  2  NM_079707.2  CG3331-RA (e), mRNA 
0   0  0  5  9  NM_135247.2  CG9138-RA (SP1070), mRNA 
0   0  0  5  8  NM_057454.3  CG16720-RA, transcript variant A (5-HT1A), mRNA 
0   0  0  5  8  NM_166322.1  CG16720-RB, transcript variant B (5-HT1A), mRNA 
0   0  0  5  4  NM_080016.2  CG4481-RA (Glu-RIB), mRNA 
0   0  0  4  7  NM_141661.1  CG8312-RA, transcript variant A (CG8312), mRNA 
0   0  0  4  6  NM_176438.1  CG8312-RB, transcript variant B (CG8312), mRNA 
0   0  0  4  6  NM_135389.1  CG13087-RA (CG13087), mRNA 
0   0  0  4  6  NM_139776.2  CG10289-RA (CG10289), mRNA 
0   0  0  4  3  NM_001031953.1  CG6128-RA, transcript variant A (CG6128), mRNA 
0   0  0  4  3  NM_140215.1  CG6128-RB, transcript variant B (CG6128), mRNA 
0   0  0  4  3  NM_001031952.1  CG11714-RA, transcript variant A (CG11714), mRNA 

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