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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6642R-1 |
| Symbol |
a10 |
Full Name |
antennal protein 10 |
| CG No |
CG6642 |
Old CG No |
CG6642 |
| Synonyms |
OS-D/A10, Phk-1, A10, OS-D, Os-D, CG6642, a10 |
| Accession No (Link to NCBI) |
NM_079397.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGGGACAGCCCGGTTTCCGT |
| Primer Seq. 3' |
aatctagaggtaccGGCTCCTCATTGGCCTTGGAC |
| Predicted Fragment Size |
464 |
| IR fragment full Seq |
0001 ATGGGACAGC CCGGTTTCCG TCGTGCCATT GGGCATTTTT CGTTGGTGGT GGCACTGATG
0061 TGCACCACCT GTTTCCAAGT GGAAGGACTA CCCCATNCCG CCGGCCACGT CGCCGTCACC
0121 AATGATGGAA AGAATGGTGG AGCAGGCCNT ACGACGATAA GTTCGACAAC GTCGATCTGG
0181 ACGAGATCCT TAACCAAGAG CGACTGTTGA TCAACTACAT AAAGTGCCTG GAGGGCACAG
0241 GTCCTTGCAC TCCCGATGCC AAGATGTTAA AGGAGATCCT GCCCGACGCC ATCCAAACTG
0301 ACTGCACCAA GTGCACGGAG AAGCAGCGGT ATGGTGCTGA AAAGGTGACC CGTCATCTCA
0361 TCGACAACCG ACCCACCGAC TGGGAGCGTC TGGAAAAGAT CTACGATCCC GAGGGAACCT
0421 ACCGCATCAA ATACCAGGAG ATGAAGTCCA AGGCCAATGA GGANCC |
| in silico PCR Fragment |
0001 ATGGGACAGC CCGGTTTCCG TCGTGCCATT GGGCACGTTT CGTTGGTGGT GGCACTGATG
0061 TGCACCACCT GTTTCCAAGT GGAAGGACTA CCCCAT-CCG CCGGCCACGT CGCCGTCACC
0121 AATGATGGAA AGAATGGTGG AGCAGGCC-T ACGACGATAA GTTCGACAAC GTCGATCTGG
0181 ACGAGATCCT TAACCAAGAG CGACTGTTGA TCAACTACAT AAAGTGCCTG GAGGGCACAG
0241 GTCCTTGCAC TCCCGATGCC AAGATGTTGA AGGAGATCCT GCCCGACGCC ATCCAAACCG
0301 ATTGCACCAA GTGCACGGAG AAGCAGCGGT ATGGTGCTGA AAAGGTGACC CGTCATCTCA
0361 TCGACAACCG ACCCACCGAC TGGGAGCGTC TGGAAAAGAT CTACGATCCC GAGGGAACCT
0421 ACCGCATCAA ATACCAGGAG ATGAAGTCCA AGGCCAATGA GGAGCC |
| Assemble Data |
6642R-1.IR_full 1 ATGGGACAGCCCGGTTTCCGTCGTGCCATTGGGCATTTTTCGTTGGTGGTGGCACTGATG 60
||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
6642R-1.in silico 1 ATGGGACAGCCCGGTTTCCGTCGTGCCATTGGGCACGTTTCGTTGGTGGTGGCACTGATG 60
6642R-1.IR_full 61 TGCACCACCTGTTTCCAAGTGGAAGGACTACCCCATNCCGCCGGCCACGTCGCCGTCACC 120
|||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
6642R-1.in silico 61 TGCACCACCTGTTTCCAAGTGGAAGGACTACCCCAT-CCGCCGGCCACGTCGCCGTCACC 120
6642R-1.IR_full 121 AATGATGGAAAGAATGGTGGAGCAGGCCNTACGACGATAAGTTCGACAACGTCGATCTGG 180
|||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
6642R-1.in silico 121 AATGATGGAAAGAATGGTGGAGCAGGCC-TACGACGATAAGTTCGACAACGTCGATCTGG 180
6642R-1.IR_full 181 ACGAGATCCTTAACCAAGAGCGACTGTTGATCAACTACATAAAGTGCCTGGAGGGCACAG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6642R-1.in silico 181 ACGAGATCCTTAACCAAGAGCGACTGTTGATCAACTACATAAAGTGCCTGGAGGGCACAG 240
6642R-1.IR_full 241 GTCCTTGCACTCCCGATGCCAAGATGTTAAAGGAGATCCTGCCCGACGCCATCCAAACTG 300
|||||||||||||||||||||||||||| ||||||||||||||||||||||||||||| |
6642R-1.in silico 241 GTCCTTGCACTCCCGATGCCAAGATGTTGAAGGAGATCCTGCCCGACGCCATCCAAACCG 300
6642R-1.IR_full 301 ACTGCACCAAGTGCACGGAGAAGCAGCGGTATGGTGCTGAAAAGGTGACCCGTCATCTCA 360
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6642R-1.in silico 301 ATTGCACCAAGTGCACGGAGAAGCAGCGGTATGGTGCTGAAAAGGTGACCCGTCATCTCA 360
6642R-1.IR_full 361 TCGACAACCGACCCACCGACTGGGAGCGTCTGGAAAAGATCTACGATCCCGAGGGAACCT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6642R-1.in silico 361 TCGACAACCGACCCACCGACTGGGAGCGTCTGGAAAAGATCTACGATCCCGAGGGAACCT 420
6642R-1.IR_full 421 ACCGCATCAAATACCAGGAGATGAAGTCCAAGGCCAATGAGGANCC 466
||||||||||||||||||||||||||||||||||||||||||| ||
6642R-1.in silico 421 ACCGCATCAAATACCAGGAGATGAAGTCCAAGGCCAATGAGGAGCC 466
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
446 |
0 |
0 |
0 |
NM_079397.2 |
CG6642-RA (a10), mRNA |
| 0 |
0 |
5 |
2 |
2 |
NM_132392.1 |
CG12641-RA (CG12641), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_167570.1 |
CG8465-RC, transcript variant C (l(1)G0222), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_167569.1 |
CG8465-RB, transcript variant B (l(1)G0222), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_132993.2 |
CG8465-RA, transcript variant A (l(1)G0222), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_132906.1 |
CG13014-RA (CG13014), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_141553.1 |
CG9770-RA (CG9770), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_140278.1 |
CG6938-RA (CG6938), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_168171.1 |
CG32397-RA (CG32397), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_001043114.1 |
CG33484-RD, transcript variant D (zormin), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_001043112.1 |
CG33484-RB, transcript variant B (zormin), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_206240.1 |
CG33484-RA, transcript variant A (zormin), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_001043113.1 |
CG33484-RC, transcript variant C (zormin), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_135533.1 |
CG5384-RA (CG5384), mRNA |
| 0 |
0 |
1 |
3 |
11 |
NM_078664.2 |
CG5870-RA (beta-Spec), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_057842.2 |
CG16858-RA (vkg), mRNA |
| 0 |
0 |
1 |
2 |
9 |
NM_080709.3 |
CG8285-RA (boss), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_132107.2 |
CG3224-RA (CG3224), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167778.1 |
CG14619-RB, transcript variant B (CG14619), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001038910.1 |
CG34001-RA (CG34001), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167777.1 |
CG14619-RC, transcript variant C (CG14619), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167775.1 |
CG14619-RD, transcript variant D (CG14619), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167776.1 |
CG14619-RE, transcript variant E (CG14619), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_134618.2 |
CG14619-RA, transcript variant A (CG14619), mRNA |
| 0 |
0 |
0 |
7 |
5 |
NM_144472.2 |
CG1898-RA (HBS1), mRNA |
| 0 |
0 |
0 |
4 |
12 |
NM_080227.1 |
CG11390-RA (PebIII), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_135850.2 |
CG8864-RA (Cyp28a5), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_078851.2 |
CG4192-RA (kek3), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_167885.1 |
CG1009-RA, transcript variant A (Psa), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_139360.2 |
CG1009-RB, transcript variant B (Psa), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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