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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6615R-3 |
| Symbol |
scaf6 |
Full Name |
scaf6 |
| CG No |
CG33522 |
Old CG No |
CG6615 |
| Synonyms |
unnamed, BP1040, CG6615, CG6626, CG14058, CG32168, CG33522, scaf6 |
| Accession No (Link to NCBI) |
NM_001014589.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Yano H, Yamamoto-Hino M, Awano W, Aoki-Kinoshita KF, Tsuda-Sakurai K, Okano H, Goto S.
Identification of proteasome components required for apical localization of Chaoptin using functional genomics.
J Neurogenet (2012) 26(1) 53-63
[
PubMed ID = 22417167
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCCACGCGATGCCAGTTTACG |
| Primer Seq. 3' |
aatctagaggtaccCGGATTGCTGGATCTGTTCCC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCCNCGCGAT GCCAGTTTAC GAAATATTAT CGACAAACTG GCGGAGTTTG TGGCCAGAAA
0061 TGGGCCGGAA TTCGAGGCGA TAACCAAAGC AGAAGCAGCA GAACAATCCA AAGTTCGAGT
0121 TCCTCTACGG CGGGGAGTTC GCCAACTATT ATCAATTCAG GGTGGCAGCG GAACAAGCTC
0181 TGCTAAAGCA ACAGGCCATT AACCAACAAC TGCCACCAGG TGCTCCATCC GGCCACTACA
0241 TGCAACATCC GCCGCCAATG CAGCAATCCC AACCACCGGG CCTCTATCCG CCACCCAATC
0301 AGCANGCCAC CGCACCCGCC CGAAACCGCA CAAGACAATA TGCAACAGCA AACACAGCAC
0361 TCCTGGCCGC CGAACTCCGG CAACGGTGGA CCGCCGAACA ATCAGCAGGC CAATGGCAAT
0421 TCCATGGCCA TGAATCTAAC GTCACAACTG GAAGCCATCA AGATGCAGCA GAAGTCGCTG
0481 CGGGAACAGA TCCAGCAATC CG |
| in silico PCR Fragment |
0001 GCCACGCGAT GCCAGTTTAC GAAATATTAT CGACAAACTG GCGGAGTTTG TGGCCAGAAA
0061 TGGGCCGGAA TTCGAGGCGA TAACC-AAGC AGAAGCAGCA GAACAATCCA AAGTTCGAGT
0121 TCCTCTACGG CGGGGAGTTC GCCAACTATT ATCAATTCAG GGTGGCAGCG GAACAAGCTC
0181 TGCTAAAGCA ACAGGCCATT AACCAACAAC TGCCACCAGG TGCTCCATCC GGCCACTACA
0241 TGCAACATCC GCCGCCAATG CAGCAATCCC AACCACCGGG CCTCTATCCG CCACCCAATC
0301 AGCA-GCCAC CGCACCCGCC CGAAACCGCA CAAGACAATA TGCAACAGCA AACACAGCAC
0361 TCCTGGCCGC CGAACTCCGG CAACGGTGGG CCGCCAAACA ATCAGCAGGC CAATGGCAAT
0421 CCCATGGCCA TGAATCTAAC GTCACAACTG GAAGCCATCA AGATGCAGCA GAAGTCGCTG
0481 CGGGAACAGA TCCAGCAATC CG |
| Assemble Data |
6615R-3.IR_full 1 GCCNCGCGATGCCAGTTTACGAAATATTATCGACAAACTGGCGGAGTTTGTGGCCAGAAA 60
||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6615R-3.in silico 1 GCCACGCGATGCCAGTTTACGAAATATTATCGACAAACTGGCGGAGTTTGTGGCCAGAAA 60
6615R-3.IR_full 61 TGGGCCGGAATTCGAGGCGATAACCAAAGCAGAAGCAGCAGAACAATCCAAAGTTCGAGT 120
||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
6615R-3.in silico 61 TGGGCCGGAATTCGAGGCGATAACC-AAGCAGAAGCAGCAGAACAATCCAAAGTTCGAGT 120
6615R-3.IR_full 121 TCCTCTACGGCGGGGAGTTCGCCAACTATTATCAATTCAGGGTGGCAGCGGAACAAGCTC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6615R-3.in silico 121 TCCTCTACGGCGGGGAGTTCGCCAACTATTATCAATTCAGGGTGGCAGCGGAACAAGCTC 180
6615R-3.IR_full 181 TGCTAAAGCAACAGGCCATTAACCAACAACTGCCACCAGGTGCTCCATCCGGCCACTACA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6615R-3.in silico 181 TGCTAAAGCAACAGGCCATTAACCAACAACTGCCACCAGGTGCTCCATCCGGCCACTACA 240
6615R-3.IR_full 241 TGCAACATCCGCCGCCAATGCAGCAATCCCAACCACCGGGCCTCTATCCGCCACCCAATC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6615R-3.in silico 241 TGCAACATCCGCCGCCAATGCAGCAATCCCAACCACCGGGCCTCTATCCGCCACCCAATC 300
6615R-3.IR_full 301 AGCANGCCACCGCACCCGCCCGAAACCGCACAAGACAATATGCAACAGCAAACACAGCAC 360
|||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
6615R-3.in silico 301 AGCA-GCCACCGCACCCGCCCGAAACCGCACAAGACAATATGCAACAGCAAACACAGCAC 360
6615R-3.IR_full 361 TCCTGGCCGCCGAACTCCGGCAACGGTGGACCGCCGAACAATCAGCAGGCCAATGGCAAT 420
||||||||||||||||||||||||||||| ||||| ||||||||||||||||||||||||
6615R-3.in silico 361 TCCTGGCCGCCGAACTCCGGCAACGGTGGGCCGCCAAACAATCAGCAGGCCAATGGCAAT 420
6615R-3.IR_full 421 TCCATGGCCATGAATCTAACGTCACAACTGGAAGCCATCAAGATGCAGCAGAAGTCGCTG 480
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6615R-3.in silico 421 CCCATGGCCATGAATCTAACGTCACAACTGGAAGCCATCAAGATGCAGCAGAAGTCGCTG 480
6615R-3.IR_full 481 CGGGAACAGATCCAGCAATCCG 502
||||||||||||||||||||||
6615R-3.in silico 481 CGGGAACAGATCCAGCAATCCG 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
6 |
NM_001014589.1 |
CG33522-RA, transcript variant A (scaf6), mRNA |
| 100 |
482 |
0 |
0 |
6 |
NM_001038939.1 |
CG33522-RB, transcript variant B (scaf6), mRNA |
| 0.41 |
2 |
4 |
3 |
5 |
NM_142054.1 |
CG14365-RA (CG14365), mRNA |
| 0 |
0 |
3 |
7 |
5 |
NM_169430.2 |
CG6939-RB, transcript variant B (Sbf), mRNA |
| 0 |
0 |
3 |
7 |
5 |
NM_058053.3 |
CG6939-RA, transcript variant A (Sbf), mRNA |
| 0 |
0 |
3 |
5 |
4 |
NM_168494.1 |
CG32098-RA (CG32098), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_142326.2 |
CG16941-RA (CG16941), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_139396.3 |
CG2069-RA (Oseg4), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_170246.1 |
CG17367-RA, transcript variant A (Lnk), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_143181.2 |
CG17367-RB, transcript variant B (Lnk), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_170247.1 |
CG17367-RC, transcript variant C (Lnk), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_132712.1 |
CG11581-RA (CG11581), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_145106.1 |
CG6964-RA, transcript variant A (Gug), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_079249.2 |
CG6964-RB, transcript variant B (Gug), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_176300.1 |
CG6964-RC, transcript variant C (Gug), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_206303.1 |
CG6964-RD, transcript variant D (Gug), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NR_001944.1 |
CR32385, miscRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_206294.1 |
CG33275-RA, transcript variant A (CG33275), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_206295.1 |
CG33275-RB, transcript variant B (CG33275), mRNA |
| 0 |
0 |
1 |
8 |
3 |
NM_176692.1 |
CG3960-RD, transcript variant D (CG3960), mRNA |
| 0 |
0 |
1 |
8 |
3 |
NM_206636.1 |
CG3960-RF, transcript variant F (CG3960), mRNA |
| 0 |
0 |
1 |
7 |
16 |
NM_078717.2 |
CG3696-RA, transcript variant A (kis), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_176226.1 |
CG15086-RC, transcript variant C (CG15086), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_176227.1 |
CG15086-RD, transcript variant D (CG15086), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_137513.2 |
CG15086-RA, transcript variant A (CG15086), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_176225.1 |
CG15086-RB, transcript variant B (CG15086), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_135374.2 |
CG13390-RA (CG13390), mRNA |
| 0 |
0 |
1 |
4 |
14 |
NM_132584.2 |
CG32654-RC (CG32654), mRNA |
| 0 |
0 |
1 |
4 |
13 |
NM_135191.2 |
CG9537-RA (DLP), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_142328.2 |
CG8907-RA (CG8907), mRNA |
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