NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 6608R-1

Symbol CG6608 Full Name CG6608

CG No CG6608 Old CG No CG6608

Synonyms CG6608

Accession No (Link to NCBI) NM_141777.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGCGGGAGCAGCTGCATCAAA

Primer Seq. 3'

aatctagaggtaccAGGTCCCTCCTGGCGGACAAT

Predicted Fragment Size

IR fragment full Seq

0001 cgcgggANCA GCTNCATCAA ATGCTGGCCG GTGGACTTTC GGCGGCCATT ACGCGCTCCA 
0061 CCTGCCAGNC GCTGGACGTG CTAAAGATCC GCTTCCAGCT GCAGGTGGAG CCGCTGGGCA 
0121 AGAACGCCGC CAAGGAGGGA CCAGGAGCCC TTACCTCCAA ATACACATCC ATCGGACAGG 
0181 CGGTGAAAAC CATATACCGC GAGGAGGGAA TGTTGGCCTT CTGGAAGGGA CACAATCCTG 
0241 CCCAAGTGCT GAGCATAATG TACGGCATTT GTCAGTTCTG GACGTACGAG CAGCTCAGCC 
0301 TGATGGCCAA ACAGACCAGC TATCTGGCGG ATCATCAGCA TCTATCGAAC TTCCTGTGCG 
0361 GCGCTGCGGC CGGAGGAGCA GCAGTGATCA TATCCACGCC GCTGGACGTC ATCCGCACTC 
0421 GTCTGATTGC CCAGGACACC AGCAAGGGAT ACAGGAATGC AACAAGGGCT GTGTCGGCGA 
0481 TTGTCCGCCA GGAGGGACCT

in silico PCR Fragment

0001 CGCGGGAGCA GCTGCATCAA ATGCTGGCCG GTGGACTTTC GGCGGCCATT ACGCGCTCCA 
0061 CCTGCCAGCC GCTGGACGTG CTAAAGATCC GCTTCCAGCT GCAGGTGGAG CCGCTGGGCA 
0121 AGAACGCCGC CAAGGAGGGA CCAGGAGCCC TTACCTCCAA ATACACATCC ATCGGACAGG 
0181 CGGTGAAAAC CATATACCGC GAGGAGGGAA TGTTGGCCTT CTGGAAGGGA CACAATCCTG 
0241 CCCAAGTGCT GAGCATAATG TACGGCATTT GTCAGTTCTG GACGTACGAG CAGCTCAGCC 
0301 TGATGGCCAA ACAGACCAGC TATCTGGCGG ATCATCAGCA TCTATCGAAC TTCCTGTGCG 
0361 GCGCTGCGGC CGGAGGAGCA GCAGTGATCA TATCCACGCC GCTGGACGTC ATCCGCACTC 
0421 GTCTGATTGC CCAGGACACC AGCAAGGGAT ACAGGAATGC AACAAGGGCT GTGTCGGCGA 
0481 TTGTCCGCCA GGAGGGACCT

Assemble Data


6608R-1.IR_full       1   CGCGGGANCAGCTNCATCAAATGCTGGCCGGTGGACTTTCGGCGGCCATTACGCGCTCCA 60
                          ||||||| ||||| ||||||||||||||||||||||||||||||||||||||||||||||
6608R-1.in silico     1   CGCGGGAGCAGCTGCATCAAATGCTGGCCGGTGGACTTTCGGCGGCCATTACGCGCTCCA 60


6608R-1.IR_full       61  CCTGCCAGNCGCTGGACGTGCTAAAGATCCGCTTCCAGCTGCAGGTGGAGCCGCTGGGCA 120
                          |||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
6608R-1.in silico     61  CCTGCCAGCCGCTGGACGTGCTAAAGATCCGCTTCCAGCTGCAGGTGGAGCCGCTGGGCA 120


6608R-1.IR_full       121 AGAACGCCGCCAAGGAGGGACCAGGAGCCCTTACCTCCAAATACACATCCATCGGACAGG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6608R-1.in silico     121 AGAACGCCGCCAAGGAGGGACCAGGAGCCCTTACCTCCAAATACACATCCATCGGACAGG 180


6608R-1.IR_full       181 CGGTGAAAACCATATACCGCGAGGAGGGAATGTTGGCCTTCTGGAAGGGACACAATCCTG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6608R-1.in silico     181 CGGTGAAAACCATATACCGCGAGGAGGGAATGTTGGCCTTCTGGAAGGGACACAATCCTG 240


6608R-1.IR_full       241 CCCAAGTGCTGAGCATAATGTACGGCATTTGTCAGTTCTGGACGTACGAGCAGCTCAGCC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6608R-1.in silico     241 CCCAAGTGCTGAGCATAATGTACGGCATTTGTCAGTTCTGGACGTACGAGCAGCTCAGCC 300


6608R-1.IR_full       301 TGATGGCCAAACAGACCAGCTATCTGGCGGATCATCAGCATCTATCGAACTTCCTGTGCG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6608R-1.in silico     301 TGATGGCCAAACAGACCAGCTATCTGGCGGATCATCAGCATCTATCGAACTTCCTGTGCG 360


6608R-1.IR_full       361 GCGCTGCGGCCGGAGGAGCAGCAGTGATCATATCCACGCCGCTGGACGTCATCCGCACTC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6608R-1.in silico     361 GCGCTGCGGCCGGAGGAGCAGCAGTGATCATATCCACGCCGCTGGACGTCATCCGCACTC 420


6608R-1.IR_full       421 GTCTGATTGCCCAGGACACCAGCAAGGGATACAGGAATGCAACAAGGGCTGTGTCGGCGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6608R-1.in silico     421 GTCTGATTGCCCAGGACACCAGCAAGGGATACAGGAATGCAACAAGGGCTGTGTCGGCGA 480


6608R-1.IR_full       481 TTGTCCGCCAGGAGGGACCT 500
                          ||||||||||||||||||||
6608R-1.in silico     481 TTGTCCGCCAGGAGGGACCT 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_169372.1	CG6608-RB, transcript variant B (CG6608), mRNA
100	482	0	0	0	NM_141777.2	CG6608-RA, transcript variant A (CG6608), mRNA
0.2	1	2	3	6	NM_142098.1	CG8538-RA (CG8538), mRNA
0	0	3	3	3	NM_139922.2	CG13676-RA (CG13676), mRNA
0	0	3	3	2	NM_137011.2	CG4688-RA (CG4688), mRNA
0	0	3	2	12	NM_079249.2	CG6964-RB, transcript variant B (Gug), mRNA
0	0	3	2	12	NM_176300.1	CG6964-RC, transcript variant C (Gug), mRNA
0	0	3	2	12	NM_206303.1	CG6964-RD, transcript variant D (Gug), mRNA
0	0	3	2	12	NM_145106.1	CG6964-RA, transcript variant A (Gug), mRNA
0	0	2	3	7	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
0	0	2	3	7	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
0	0	2	3	7	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
0	0	2	2	4	NM_165354.1	CG9264-RB, transcript variant B (CG9264), mRNA
0	0	1	11	12	NM_140605.1	CG13047-RA (CG13047), mRNA
0	0	1	6	5	NM_079687.2	CG10888-RA (Rh3), mRNA
0	0	1	4	7	NM_206123.1	CG33464-RA (CG33464), mRNA
0	0	1	4	5	NM_143593.2	CG1542-RA (CG1542), mRNA
0	0	1	4	4	NM_137048.2	CG18369-RA (CG18369), mRNA
0	0	1	4	4	NM_168538.1	CG32118-RA (CG32118), mRNA
0	0	1	4	2	NM_133163.3	CG9397-RA (1.28), mRNA
0	0	1	4	2	NM_135603.1	CG6737-RA (CG6737), mRNA
0	0	1	3	5	NM_169386.1	CG17228-RA, transcript variant A (pros), mRNA
0	0	1	3	4	NM_164443.1	CG31665-RA, transcript variant A (CG31665), mRNA
0	0	1	3	4	NM_205889.1	CG31665-RB, transcript variant B (CG31665), mRNA
0	0	1	3	4	NM_079593.3	CG17228-RC, transcript variant C (pros), mRNA
0	0	1	3	4	NM_176459.1	CG17228-RD, transcript variant D (pros), mRNA
0	0	1	3	4	NM_133136.1	CG7874-RA (CG7874), mRNA
0	0	1	3	3	NM_142635.2	CG5180-RA (CG5180), mRNA
0	0	1	3	2	NM_078700.2	CG1849-RA (run), mRNA
0	0	1	3	2	NM_168220.1	CG8532-RD, transcript variant D (lqf), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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