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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 6598R-1 
 Symbol Fdh  Full Name Formaldehyde dehydrogenase 
 CG No CG6598  Old CG No CG6598 
 Synonyms ODH, Odh, CG6598, gfd/odh, ADHX, odh, gfd, Fdh 
 Accession No (Link to NCBI) NM_079586.2 
 Inserted Chr. X 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCAAAGAAACCGCTGGTCATCG 
 Primer Seq. 3' 
aatctagaggtaccGGCTCCGTAGCCTGTGGAAAT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CAAAGAAACC GCTGGTCATC GAGGATATCG AGGTGGCGCC GCCcaAGGNT CATGAGGTGC 
0061 GCATCAAGAT CACGGCCACG GGCGTCTGCC ACACGGATGC CTTCACCCTG AGCGGTGCCG 
0121 ATCCGGAGGG CCTGTTCCCA GGTGGTGTTG GGACATGAGG GCGCCGGCNA TCGTGGAGAG 
0181 CGTCGGCGAN GGGCGTGACC AACTTCAAGG CTGGCGACCA TGTCATCGCT CTCTACATCC 
0241 CCCAGTGCAA CGAGNTGCAA GTTCTGCAAG AGCGGCAAGA CGAATCTCTG CCAAAAGATC 
0301 CGACTTACCC AGGGCGCTGG AGTCATGCCC GAGGGCACCT CCCGTCTCAG CTGCAAGGGA 
0361 CAGCAGTTGT TCCACTTCAT GGGCACCTCC ACCTTCGCAG AGTACACCGT GGTGGCCGAC 
0421 ATTTCGCTGA CAAAGATCAA CGAAAAGGCG CCGCTGGAGA AAGTCTGCCT GCTGGGGTGC 
0481 GGCATTTCCA CAGGCTACGG AGCC 
 in silico PCR Fragment 
0001 CAAAGAAACC GCTGGTCATC GAGGATATCG AGGTGGCGCC GCCCAAGGCT CATGAGGTGC 
0061 GCATCAAGAT CACGGCCACG GGCGTCTGCC ACACGGATGC CTTCACCCTG AGCGGTGCCG 
0121 ATCCGGAGGG CCTGTTCCCA -GTGGTGTTG GGACATGAGG GCGCCGGC-A TCGTGGAGAG 
0181 CGTCGGCGA- GGGCGTGACC AACTTCAAGG CTGGCGACCA TGTCATCGCT CTCTACATCC 
0241 CCCAGTGCAA CGAG-TGCAA GTTCTGCAAG AGCGGCAAGA CGAATCTCTG CCAAAAGATC 
0301 CGACTTACCC AGGGCGCTGG AGTCATGCCC GAGGGCACCT CCCGTCTCAG CTGCAAGGGA 
0361 CAGCAGTTGT TCCACTTCAT GGGCACCTCC ACCTTCGCAG AGTACACCGT GGTGGCCGAC 
0421 ATTTCGCTGA CAAAGATCAA CGAAAAGGCG CCGCTGGAGA AAGTCTGCCT GCTGGGGTGC 
0481 GGCATTTCCA CAGGCTACGG AGCC 
 Assemble Data 

6598R-1.IR_full       1   CAAAGAAACCGCTGGTCATCGAGGATATCGAGGTGGCGCCGCCCAAGGNTCATGAGGTGC 60
                          |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
6598R-1.in silico     1   CAAAGAAACCGCTGGTCATCGAGGATATCGAGGTGGCGCCGCCCAAGGCTCATGAGGTGC 60


6598R-1.IR_full       61  GCATCAAGATCACGGCCACGGGCGTCTGCCACACGGATGCCTTCACCCTGAGCGGTGCCG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6598R-1.in silico     61  GCATCAAGATCACGGCCACGGGCGTCTGCCACACGGATGCCTTCACCCTGAGCGGTGCCG 120


6598R-1.IR_full       121 ATCCGGAGGGCCTGTTCCCAGGTGGTGTTGGGACATGAGGGCGCCGGCNATCGTGGAGAG 180
                          |||||||||||||||||||| ||||||||||||||||||||||||||| |||||||||||
6598R-1.in silico     121 ATCCGGAGGGCCTGTTCCCA-GTGGTGTTGGGACATGAGGGCGCCGGC-ATCGTGGAGAG 180


6598R-1.IR_full       181 CGTCGGCGANGGGCGTGACCAACTTCAAGGCTGGCGACCATGTCATCGCTCTCTACATCC 240
                          ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
6598R-1.in silico     181 CGTCGGCGA-GGGCGTGACCAACTTCAAGGCTGGCGACCATGTCATCGCTCTCTACATCC 240


6598R-1.IR_full       241 CCCAGTGCAACGAGNTGCAAGTTCTGCAAGAGCGGCAAGACGAATCTCTGCCAAAAGATC 300
                          |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
6598R-1.in silico     241 CCCAGTGCAACGAG-TGCAAGTTCTGCAAGAGCGGCAAGACGAATCTCTGCCAAAAGATC 300


6598R-1.IR_full       301 CGACTTACCCAGGGCGCTGGAGTCATGCCCGAGGGCACCTCCCGTCTCAGCTGCAAGGGA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6598R-1.in silico     301 CGACTTACCCAGGGCGCTGGAGTCATGCCCGAGGGCACCTCCCGTCTCAGCTGCAAGGGA 360


6598R-1.IR_full       361 CAGCAGTTGTTCCACTTCATGGGCACCTCCACCTTCGCAGAGTACACCGTGGTGGCCGAC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6598R-1.in silico     361 CAGCAGTTGTTCCACTTCATGGGCACCTCCACCTTCGCAGAGTACACCGTGGTGGCCGAC 420


6598R-1.IR_full       421 ATTTCGCTGACAAAGATCAACGAAAAGGCGCCGCTGGAGAAAGTCTGCCTGCTGGGGTGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6598R-1.in silico     421 ATTTCGCTGACAAAGATCAACGAAAAGGCGCCGCTGGAGAAAGTCTGCCTGCTGGGGTGC 480


6598R-1.IR_full       481 GGCATTTCCACAGGCTACGGAGCC 504
                          ||||||||||||||||||||||||
6598R-1.in silico     481 GGCATTTCCACAGGCTACGGAGCC 504



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_079586.2  CG6598-RA (Fdh), mRNA 
0   0  3  3  5  NM_137871.2  CG3536-RA (CG3536), mRNA 
0   0  3  3  5  NM_079917.2  CG18296-RA (axo), mRNA 
0   0  2  2  4  NM_131975.3  CG32767-RA, transcript variant A (CG32767), mRNA 
0   0  2  2  4  NM_206628.1  CG32767-RB, transcript variant B (CG32767), mRNA 
0   0  1  4  3  NM_140900.2  CG8025-RA (Mtr3), mRNA 
0   0  1  3  8  NM_137983.3  CG9850-RA, transcript variant A (CG9850), mRNA 
0   0  1  3  2  NM_164727.1  CG31908-RB, transcript variant B (CG31908), mRNA 
0   0  1  3  2  NM_164726.1  CG31908-RA, transcript variant A (CG31908), mRNA 
0   0  1  3  2  NM_079687.2  CG10888-RA (Rh3), mRNA 
0   0  1  2  7  NM_132562.1  CG12720-RA (CG12720), mRNA 
0   0  1  2  4  NM_135760.1  CG5204-RA (CG5204), mRNA 
0   0  1  2  3  NM_130542.2  CG11403-RA (CG11403), mRNA 
0   0  1  2  3  NM_080097.2  CG6699-RA (beta'Cop), mRNA 
0   0  0  5  12  NM_079903.2  CG15319-RB (nej), mRNA 
0   0  0  5  6  NM_135077.2  CG14023-RA (CG14023), mRNA 
0   0  0  5  5  NM_001043163.1  CG40300-RA, transcript variant A (CG40300), mRNA 
0   0  0  5  5  NM_001043164.1  CG40300-RB, transcript variant B (CG40300), mRNA 
0   0  0  5  5  NM_001043162.1  CG40300-RC, transcript variant C (CG40300), mRNA 
0   0  0  5  3  NM_137726.2  CG10069-RA, transcript variant A (CG10069), mRNA 
0   0  0  5  3  NM_166434.1  CG10069-RB, transcript variant B (CG10069), mRNA 
0   0  0  5  3  NM_166435.1  CG10069-RC, transcript variant C (CG10069), mRNA 
0   0  0  4  11  NM_132508.2  CG1703-RA (CG1703), mRNA 
0   0  0  4  11  NM_164690.1  CG9535-RB, transcript variant B (CG9535), mRNA 
0   0  0  4  11  NM_135188.2  CG9535-RA, transcript variant A (CG9535), mRNA 
0   0  0  4  9  NM_079845.2  CG7951-RA (sima), mRNA 
0   0  0  4  6  NM_078822.2  CG6320-RA, transcript variant A (Ca-beta), mRNA 
0   0  0  4  6  NM_205963.1  CG6320-RC, transcript variant C (Ca-beta), mRNA 
0   0  0  4  6  NM_205962.1  CG6320-RD, transcript variant D (Ca-beta), mRNA 
0   0  0  4  5  NM_206190.1  CG15669-RE, transcript variant E (MESK2), mRNA 

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