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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 6597R-3 
 Symbol CG6597  Full Name CG6597 
 CG No CG6597  Old CG No CG6597 
 Synonyms CG6597 
 Accession No (Link to NCBI) NM_140947.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGATCATGGTCAACCGAAGAG 
 Primer Seq. 3' 
aatctagaggtaccACTGTGGATACAGAGGCCAAT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGATCATGGT CAACCGAAGA GGGCCCGAAC CAGCTACACC AGCATACCCA CACAACAGAG 
0061 CAGCAGACGC CATATCAGAT CGGAAGATGG CTTCAGTCAA AAGCGTTGCA TGGCCTGGTT 
0121 CCAGGAGTAC ACAACACCAG ATGAACCGGA GACATTGGGT CCCGATGGCA TGGAAAAATT 
0181 CTGCGAGGAT ATCGGCGTGG AGCCCGAAAA TATTGTGATG CTGGTGCTGG CCTACAAAAT 
0241 GGGCGCCACC CAGATGGGCT TCTTCAGCCA GCAGGAGTGG CTAAAGGGCC TCACAGAACT 
0301 CGACTGCGAT TCGGCAGCGA AAATGGTTGT GAAATTGGAC TATCTGCGCA GCATACNTCA 
0361 ACGATCCCAA CTCCTTTAAA AGCATCTACA GATACGCCTA CGACTTTGCC AAGGACTCGG 
0421 ACCAACGGTG TATGGATATA CTCACTGCCA AAGCCATGCT TCAACTACTT TTGGGCAAAC 
0481 ATTGGCCTCT GTATCCACAG T 
 in silico PCR Fragment 
0001 GGATCATGGT CAACCGAAGA GGGCCCGAAC CAGCTACACC AGCATACCCA CACAACAGAG 
0061 CAGCAGACGC CATATCAGAT TGGAAGATGG CTTCAGTCAA AAGCGTTGCA TGGCCTGGTT 
0121 CCAGGAGTAC ACAACACCAG ATGAACCGGA GACATTGGGT CCCGATGGCA TGGAAAAATT 
0181 CTGCGAGGAT ATCGGCGTGG AGCCCGAGAA TATTGTGATG CTGGTGCTGG CCTACAAAAT 
0241 GGGCGCCACC CAGATGGGCT TCTTCAGCCA GCAGGAGTGG CTAAAGGGCC TCACAGAACT 
0301 CGACTGCGAT TCGGCAGCGA AAATGGTTGT GAAATTGGAC TATCTGCGCA GCATAC-TCA 
0361 ACGATCCCAA CTCCTTTAAA AGCATCTACA GATACGCCTA CGACTTTGCC AAGGACTCGG 
0421 ACCAACGGTG TATGGATATA CTCACTGCCA AAGCCATGCT TCAACTACTT TTGGGCAAAC 
0481 ATTGGCCTCT GTATCCACAG T 
 Assemble Data 

6597R-3.IR_full       1   GGATCATGGTCAACCGAAGAGGGCCCGAACCAGCTACACCAGCATACCCACACAACAGAG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6597R-3.in silico     1   GGATCATGGTCAACCGAAGAGGGCCCGAACCAGCTACACCAGCATACCCACACAACAGAG 60


6597R-3.IR_full       61  CAGCAGACGCCATATCAGATCGGAAGATGGCTTCAGTCAAAAGCGTTGCATGGCCTGGTT 120
                          |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
6597R-3.in silico     61  CAGCAGACGCCATATCAGATTGGAAGATGGCTTCAGTCAAAAGCGTTGCATGGCCTGGTT 120


6597R-3.IR_full       121 CCAGGAGTACACAACACCAGATGAACCGGAGACATTGGGTCCCGATGGCATGGAAAAATT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6597R-3.in silico     121 CCAGGAGTACACAACACCAGATGAACCGGAGACATTGGGTCCCGATGGCATGGAAAAATT 180


6597R-3.IR_full       181 CTGCGAGGATATCGGCGTGGAGCCCGAAAATATTGTGATGCTGGTGCTGGCCTACAAAAT 240
                          ||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
6597R-3.in silico     181 CTGCGAGGATATCGGCGTGGAGCCCGAGAATATTGTGATGCTGGTGCTGGCCTACAAAAT 240


6597R-3.IR_full       241 GGGCGCCACCCAGATGGGCTTCTTCAGCCAGCAGGAGTGGCTAAAGGGCCTCACAGAACT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6597R-3.in silico     241 GGGCGCCACCCAGATGGGCTTCTTCAGCCAGCAGGAGTGGCTAAAGGGCCTCACAGAACT 300


6597R-3.IR_full       301 CGACTGCGATTCGGCAGCGAAAATGGTTGTGAAATTGGACTATCTGCGCAGCATACNTCA 360
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
6597R-3.in silico     301 CGACTGCGATTCGGCAGCGAAAATGGTTGTGAAATTGGACTATCTGCGCAGCATAC-TCA 360


6597R-3.IR_full       361 ACGATCCCAACTCCTTTAAAAGCATCTACAGATACGCCTACGACTTTGCCAAGGACTCGG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6597R-3.in silico     361 ACGATCCCAACTCCTTTAAAAGCATCTACAGATACGCCTACGACTTTGCCAAGGACTCGG 420


6597R-3.IR_full       421 ACCAACGGTGTATGGATATACTCACTGCCAAAGCCATGCTTCAACTACTTTTGGGCAAAC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6597R-3.in silico     421 ACCAACGGTGTATGGATATACTCACTGCCAAAGCCATGCTTCAACTACTTTTGGGCAAAC 480


6597R-3.IR_full       481 ATTGGCCTCTGTATCCACAGT 501
                          |||||||||||||||||||||
6597R-3.in silico     481 ATTGGCCTCTGTATCCACAGT 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_140947.1  CG6597-RA, transcript variant A (CG6597), mRNA 
100   482  0  0  0  NM_168842.1  CG6597-RB, transcript variant B (CG6597), mRNA 
0   0  3  3  3  NM_080531.2  CG5481-RA (lea), mRNA 
0   0  2  4  2  NM_001043277.1  CG31163-RD, transcript variant D (CG31163), mRNA 
0   0  2  4  2  NM_169997.3  CG31163-RB, transcript variant B (CG31163), mRNA 
0   0  2  3  2  NM_176436.1  CG8176-RB, transcript variant B (CG8176), mRNA 
0   0  2  3  2  NM_176435.1  CG8176-RA, transcript variant A (CG8176), mRNA 
0   0  1  4  2  NM_141676.2  CG12950-RA (CG12950), mRNA 
0   0  1  4  2  NM_140008.1  CG5741-RA (CG5741), mRNA 
0   0  1  3  5  NM_136458.2  CG30494-RA (CG30494), mRNA 
0   0  1  3  3  NM_132423.1  CG11556-RA (Rph), mRNA 
0   0  1  3  2  NM_001038732.1  CG12598-RC, transcript variant C (Adar), mRNA 
0   0  1  3  2  NM_130584.2  CG12598-RA, transcript variant A (Adar), mRNA 
0   0  1  3  2  NM_166903.1  CG12598-RB, transcript variant B (Adar), mRNA 
0   0  1  2  15  NM_134519.1  CG11940-RA, transcript variant A (CG11940), mRNA 
0   0  1  2  4  NM_168468.1  CG32091-RB (CG32091), mRNA 
0   0  1  2  4  NM_135264.1  CG4969-RA (Wnt6), mRNA 
0   0  1  2  2  NM_078605.1  CG6211-RA (gce), mRNA 
0   0  0  5  14  NM_169775.1  CG7467-RA, transcript variant A (osa), mRNA 
0   0  0  5  14  NM_079668.2  CG7467-RB, transcript variant B (osa), mRNA 
0   0  0  5  4  NM_168153.1  CG32406-RA (CG32406), mRNA 
0   0  0  5  4  NM_137699.3  CG10531-RA (CG10531), mRNA 
0   0  0  5  3  NM_137132.2  CG10139-RA (CG10139), mRNA 
0   0  0  5  2  NM_165793.1  CG11883-RA, transcript variant A (CG11883), mRNA 
0   0  0  5  2  NM_136754.3  CG11883-RB, transcript variant B (CG11883), mRNA 
0   0  0  4  10  NM_206506.1  CG7467-RC, transcript variant C (osa), mRNA 
0   0  0  4  9  NM_136533.1  CG11641-RA (pdm3), mRNA 
0   0  0  4  7  NM_132651.2  CG1640-RA, transcript variant A (CG1640), mRNA 
0   0  0  4  7  NM_167369.1  CG1640-RF, transcript variant F (CG1640), mRNA 
0   0  0  4  7  NM_167368.1  CG1640-RE, transcript variant E (CG1640), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
+ 
DC
+ 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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