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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 6577R-3 
 Symbol can  Full Name cannonball 
 CG No CG6577  Old CG No CG6577 
 Synonyms dTAF5L, dTAF[[II]]80, dmTAF5b, TAF5L, CG6577, can 
 Accession No (Link to NCBI) NM_080088.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGCAGCTCGGAGTCCGATAAA 
 Primer Seq. 3' 
aatctagaggtaccGAAACTTCGCCCTTGTCAACG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGCAGCTCGG AGTCCGATAA AATTAGCGAT TCAGATGATT CCAACTCAGA TGGATCGCCC 
0061 ACCGAATGGA TTAACAGAAT AAAGAATGTC AATCTATTTC CATTCAATAA AAGTGCTGAA 
0121 GATGAAAAAC CAAAGGCTTG TGAAGAACTT CCCTCAATAC CAGTTTTTAC TGGTCCGATG 
0181 CCATCGATGC CGATTTATGA TGAAGAAACC TGTCAATTTG AAATGGTGCC ACCCATCATA 
0241 CAACCAGACT TAATTAAGGA AGTAACTGAC GCAGAAATGC CAATTGAGTT GGATAAATCC 
0301 GATTCTGAGT TGGAAAAAGA AAAGTTAAAA GCCGATGAGG ATGACAACTT TTCGGACAGT 
0361 AACTTCAGTT CCAGTTTTAG TTTGAGCAGT TTAGATGATT ATGATTATGG TGAAACTGAT 
0421 CTTGATGAAC AGCATAACGA GAATAAATGT ACTTACTGGG AGGAAATGTC ATTGCGTCCC 
0481 GTTGACAAGG GCGAAGTTTC  
 in silico PCR Fragment 
0001 AGCAGCTCGG AGTCCGATAA AATTAGCGAT TCAGATGATT CCAACTCAGA TGGATCGCCC 
0061 ACCGAATGGA TTAACAGAAT AAAGAATGTC AATCTATTTC CATTCAATAA AAGTGCTGAA 
0121 GATGAAAAAC CAAAGGCTTG TGAAGAACTT CCCTCAATAC CAGTTTTTAC TGGTCCGATG 
0181 CCATCGATGC CGATTTATGA TGAAGAAACC TGTCAATTTG AAATGGTGCC ACCCATCATA 
0241 CAACCAGACT TAATTAAGGA AGTAACTGAC GCAGAAATGC CAATTGAGTT GGATAAATCC 
0301 GATTCTGAGT TGGAAAAAGA AAAGTTAAAA GCCGATGAGG ATGACAACTT TTCGGACAGT 
0361 AACACCAGTT CCAGTTTTAG TTTGAGCAGT TTAGATGATT ATGATTATGG TGAAACTGAT 
0421 CTTGATGAAC AGCATAACGA GAATAAATGT ACTTACTGGG AGGAAATGTC ATTGCGTCCC 
0481 GTTGACAAGG GCGAAGTTTC  
 Assemble Data 

6577R-3.IR_full       1   AGCAGCTCGGAGTCCGATAAAATTAGCGATTCAGATGATTCCAACTCAGATGGATCGCCC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6577R-3.in silico     1   AGCAGCTCGGAGTCCGATAAAATTAGCGATTCAGATGATTCCAACTCAGATGGATCGCCC 60


6577R-3.IR_full       61  ACCGAATGGATTAACAGAATAAAGAATGTCAATCTATTTCCATTCAATAAAAGTGCTGAA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6577R-3.in silico     61  ACCGAATGGATTAACAGAATAAAGAATGTCAATCTATTTCCATTCAATAAAAGTGCTGAA 120


6577R-3.IR_full       121 GATGAAAAACCAAAGGCTTGTGAAGAACTTCCCTCAATACCAGTTTTTACTGGTCCGATG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6577R-3.in silico     121 GATGAAAAACCAAAGGCTTGTGAAGAACTTCCCTCAATACCAGTTTTTACTGGTCCGATG 180


6577R-3.IR_full       181 CCATCGATGCCGATTTATGATGAAGAAACCTGTCAATTTGAAATGGTGCCACCCATCATA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6577R-3.in silico     181 CCATCGATGCCGATTTATGATGAAGAAACCTGTCAATTTGAAATGGTGCCACCCATCATA 240


6577R-3.IR_full       241 CAACCAGACTTAATTAAGGAAGTAACTGACGCAGAAATGCCAATTGAGTTGGATAAATCC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6577R-3.in silico     241 CAACCAGACTTAATTAAGGAAGTAACTGACGCAGAAATGCCAATTGAGTTGGATAAATCC 300


6577R-3.IR_full       301 GATTCTGAGTTGGAAAAAGAAAAGTTAAAAGCCGATGAGGATGACAACTTTTCGGACAGT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6577R-3.in silico     301 GATTCTGAGTTGGAAAAAGAAAAGTTAAAAGCCGATGAGGATGACAACTTTTCGGACAGT 360


6577R-3.IR_full       361 AACTTCAGTTCCAGTTTTAGTTTGAGCAGTTTAGATGATTATGATTATGGTGAAACTGAT 420
                          |||  |||||||||||||||||||||||||||||||||||||||||||||||||||||||
6577R-3.in silico     361 AACACCAGTTCCAGTTTTAGTTTGAGCAGTTTAGATGATTATGATTATGGTGAAACTGAT 420


6577R-3.IR_full       421 CTTGATGAACAGCATAACGAGAATAAATGTACTTACTGGGAGGAAATGTCATTGCGTCCC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6577R-3.in silico     421 CTTGATGAACAGCATAACGAGAATAAATGTACTTACTGGGAGGAAATGTCATTGCGTCCC 480


6577R-3.IR_full       481 GTTGACAAGGGCGAAGTTTC 500
                          ||||||||||||||||||||
6577R-3.in silico     481 GTTGACAAGGGCGAAGTTTC 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  8  NM_080088.1  CG6577-RA (can), mRNA 
0   0  2  2  5  NM_168980.1  CG11100-RC, transcript variant C (Mes2), mRNA 
0   0  2  2  5  NM_168979.2  CG11100-RB, transcript variant B (Mes2), mRNA 
0   0  1  3  4  NM_135954.1  CG13260-RA (CG13260), mRNA 
0   0  1  2  4  NM_080486.2  CG1966-RA (Acf1), mRNA 
0   0  1  2  4  NM_134895.2  CG3605-RA (CG3605), mRNA 
0   0  1  2  3  NM_167322.3  CG32652-RA (CG32652), mRNA 
0   0  1  2  2  NM_079582.2  CG6547-RA (mRpL37), mRNA 
0   0  0  5  6  NM_134786.4  CG7337-RA, transcript variant A (CG7337), mRNA 
0   0  0  5  6  NM_001042862.1  CG7337-RC, transcript variant C (CG7337), mRNA 
0   0  0  5  6  NM_001042863.1  CG7337-RB, transcript variant B (CG7337), mRNA 
0   0  0  4  5  NM_142560.2  CG17752-RA (CG17752), mRNA 
0   0  0  4  4  NM_001014545.1  CG33519-RB (Unc-89), mRNA 
0   0  0  4  3  NM_164468.1  CG31686-RA (CG31686), mRNA 
0   0  0  4  3  NM_134474.4  CG32532-RA (CG32532), mRNA 
0   0  0  3  9  NM_001032051.1  CG33715-RD, transcript variant D (Msp-300), mRNA 
0   0  0  3  6  NM_136741.2  CG11763-RC, transcript variant C (micr), mRNA 
0   0  0  3  6  NM_165770.1  CG11763-RA, transcript variant A (micr), mRNA 
0   0  0  3  6  NM_001038849.1  CG11763-RD, transcript variant D (micr), mRNA 
0   0  0  3  6  NM_167760.1  CG14476-RE, transcript variant E (CG14476), mRNA 
0   0  0  3  5  NM_130557.2  CG14786-RA (CG14786), mRNA 
0   0  0  3  5  NM_164382.1  CG3935-RA (al), mRNA 
0   0  0  3  5  NM_132294.2  CG32707-RA (APC4), mRNA 
0   0  0  3  4  NM_165304.1  CG10084-RB, transcript variant B (swm), mRNA 
0   0  0  3  4  NM_136132.4  CG10084-RA, transcript variant A (swm), mRNA 
0   0  0  3  3  NM_206670.1  CG33224-RA (CG33224), mRNA 
0   0  0  3  3  NM_164521.1  CG3542-RB, transcript variant B (CG3542), mRNA 
0   0  0  3  3  NM_134894.2  CG3542-RA, transcript variant A (CG3542), mRNA 
0   0  0  3  3  NM_135539.2  CG5034-RA (GATAd), mRNA 
0   0  0  3  3  NM_165375.2  CG31623-RA (dtr), mRNA 

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