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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6565R-2 |
| Symbol |
CG6565 |
Full Name |
CG6565 |
| CG No |
CG6565 |
Old CG No |
CG6565 |
| Synonyms |
CG6565 |
| Accession No (Link to NCBI) |
NM_135800.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
late pupal lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Álvarez-Fernández C, Tamirisa S, Prada F, Chernomoretz A, Podhajcer O, Blanco E, Martín-Blanco E.
Identification and functional analysis of healing regulators in Drosophila.
PLoS Genet (2015) 11(2) e1004965
[
PubMed ID = 25647511
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGGCCATCAAGAACCAGTCCC |
| Primer Seq. 3' |
aatctagaggtaccCACGTCCAGATCCAACGGTCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGGCCATCAA GAACCAGTCC CGAGAAACGC TGCGTGCCTG GAGCTACCAA TGCGAGTCGA
0061 TTTTCGCCCA GCGCTCgCGT CGCGTTCAGC AGTTGTTCAG TTTCTACCAG TCGGTGTATG
0121 GAACTCAGGG TCTGAAGCAG CTATGGCGCG CCNTACTACC GCCGGGANGC TGCAGCCTCC
0181 GCTGCGGGGA ATGGTGATGA GTGCAGTGGG TCTCGTCGGG CTGAATATCA AGCGAATGGG
0241 TAGCGATGGC TTCGATTGGA ACAAGGAGCG CCTGGCCTTG TCCAACTTTG ATCTGTGCCG
0301 CCGGGATATT GAGTTTATCA ACNGCCCTGC CCAACAATCA GCTGTNCGAC CGCTGCCAGG
0361 CCAAGAAGAT GAAATACTGC TACTGTCAGC TGGGCAACCA NNGCTGCAAA AAGGGCCAAT
0421 CCCAGCCGTC CACCAGCAAA GAGGCGGAGA CGGATCCGGA CACCAGCATC AGCAATTgTC
0481 TGCGANCGTT GGATCTGGAC GTG |
| in silico PCR Fragment |
0001 TGGCCATCAA GAACCAGTCC CGAGAAACGC TGCGTGCCTG GAGCTACCAA TGCGAGTCGA
0061 TTTTCGCCCA GCGCTCGCGT CGCGTTCAGC AGTTGTTCAG TTTCTACCAG TCGGTGTATG
0121 GAACTCAGGG TCTGAAGCAG CTATGGCGCG CC-TACTACC GCCGGGA-GC TGCAGCCTCC
0181 GCTGCGGGGA ATGGTGATGA GTGCAGTGGG TCTCGTCGGG CTGAATATCA AGCGAATGGG
0241 TAGCGATGGC TTCGATTGGA ACAAGGAGCG CCTGGCCTTG TCCAACTTTG ATCTGTGCCG
0301 CCGGGATATT GAGTTTATCA AC-GCCCTGC CCAACAATCA GCTGTGCGAC CGCTGCCAGG
0361 CCAAGAAGAT GAAATACTGC TACTGTCAGC TGGGCAACCA GCGCTGCAAA AAGGGCCAAT
0421 CCCAGCCGTC CACCAGCAAA GAGGCGGAGA CGGATCCGGA CACCAGCATC AGCAATTGTC
0481 TGCGACCGTT GGATCTGGAC GTG |
| Assemble Data |
6565R-2.IR_full 1 TGGCCATCAAGAACCAGTCCCGAGAAACGCTGCGTGCCTGGAGCTACCAATGCGAGTCGA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6565R-2.in silico 1 TGGCCATCAAGAACCAGTCCCGAGAAACGCTGCGTGCCTGGAGCTACCAATGCGAGTCGA 60
6565R-2.IR_full 61 TTTTCGCCCAGCGCTCGCGTCGCGTTCAGCAGTTGTTCAGTTTCTACCAGTCGGTGTATG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6565R-2.in silico 61 TTTTCGCCCAGCGCTCGCGTCGCGTTCAGCAGTTGTTCAGTTTCTACCAGTCGGTGTATG 120
6565R-2.IR_full 121 GAACTCAGGGTCTGAAGCAGCTATGGCGCGCCNTACTACCGCCGGGANGCTGCAGCCTCC 180
|||||||||||||||||||||||||||||||| |||||||||||||| ||||||||||||
6565R-2.in silico 121 GAACTCAGGGTCTGAAGCAGCTATGGCGCGCC-TACTACCGCCGGGA-GCTGCAGCCTCC 180
6565R-2.IR_full 181 GCTGCGGGGAATGGTGATGAGTGCAGTGGGTCTCGTCGGGCTGAATATCAAGCGAATGGG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6565R-2.in silico 181 GCTGCGGGGAATGGTGATGAGTGCAGTGGGTCTCGTCGGGCTGAATATCAAGCGAATGGG 240
6565R-2.IR_full 241 TAGCGATGGCTTCGATTGGAACAAGGAGCGCCTGGCCTTGTCCAACTTTGATCTGTGCCG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6565R-2.in silico 241 TAGCGATGGCTTCGATTGGAACAAGGAGCGCCTGGCCTTGTCCAACTTTGATCTGTGCCG 300
6565R-2.IR_full 301 CCGGGATATTGAGTTTATCAACNGCCCTGCCCAACAATCAGCTGTNCGACCGCTGCCAGG 360
|||||||||||||||||||||| |||||||||||||||||||||| ||||||||||||||
6565R-2.in silico 301 CCGGGATATTGAGTTTATCAAC-GCCCTGCCCAACAATCAGCTGTGCGACCGCTGCCAGG 360
6565R-2.IR_full 361 CCAAGAAGATGAAATACTGCTACTGTCAGCTGGGCAACCANNGCTGCAAAAAGGGCCAAT 420
|||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
6565R-2.in silico 361 CCAAGAAGATGAAATACTGCTACTGTCAGCTGGGCAACCAGCGCTGCAAAAAGGGCCAAT 420
6565R-2.IR_full 421 CCCAGCCGTCCACCAGCAAAGAGGCGGAGACGGATCCGGACACCAGCATCAGCAATTGTC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6565R-2.in silico 421 CCCAGCCGTCCACCAGCAAAGAGGCGGAGACGGATCCGGACACCAGCATCAGCAATTGTC 480
6565R-2.IR_full 481 TGCGANCGTTGGATCTGGACGTG 503
||||| |||||||||||||||||
6565R-2.in silico 481 TGCGACCGTTGGATCTGGACGTG 503
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_135800.2 |
CG6565-RA (CG6565), mRNA |
| 0.2 |
1 |
3 |
4 |
6 |
NM_166535.1 |
CG4444-RA (px), mRNA |
| 0 |
0 |
4 |
4 |
8 |
NM_167474.1 |
CG8909-RB (CG8909), mRNA |
| 0 |
0 |
2 |
7 |
7 |
NM_165961.1 |
CG3845-RA, transcript variant A (l(2)01424), mRNA |
| 0 |
0 |
2 |
7 |
7 |
NM_001038854.1 |
CG3845-RC, transcript variant C (l(2)01424), mRNA |
| 0 |
0 |
2 |
7 |
7 |
NM_143770.2 |
CG3845-RB, transcript variant B (l(2)01424), mRNA |
| 0 |
0 |
2 |
4 |
8 |
NM_144448.2 |
CG1915-RC, transcript variant C (sls), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_079937.2 |
CG1915-RA, transcript variant A (sls), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_142060.3 |
CG9286-RA (CG9286), mRNA |
| 0 |
0 |
2 |
2 |
10 |
NM_143408.2 |
CG1401-RA (cul-5), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_001038863.1 |
CG33960-RA (CG33960), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_132259.2 |
CG11219-RA (PIP82), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_079702.2 |
CG3637-RA (Cortactin), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_142268.1 |
CG6006-RA (CG6006), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_142123.1 |
CG3505-RA (CG3505), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_136375.1 |
CG3273-RA (sced), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_132634.2 |
CG12096-RA (CG12096), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_206737.1 |
CG10545-RE, transcript variant E (Gbeta13F), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_140280.2 |
CG5688-RA (Grip163), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166792.1 |
CG11153-RA, transcript variant A (Sox102F), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001014695.1 |
CG11153-RB, transcript variant B (Sox102F), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_136409.1 |
CG17002-RB (CG17002), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_141697.1 |
CG9461-RA (CG9461), mRNA |
| 0 |
0 |
0 |
5 |
16 |
NM_206138.1 |
CG15920-RB, transcript variant B (resilin), mRNA |
| 0 |
0 |
0 |
5 |
16 |
NM_137313.2 |
CG15920-RA, transcript variant A (resilin), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_143610.2 |
CG11337-RA, transcript variant A (CG11337), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_170550.1 |
CG11337-RB, transcript variant B (CG11337), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_205922.1 |
CG33296-RA (CG33296), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_169206.1 |
CG9656-RA (grn), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_143344.2 |
CG5520-RA (Gp93), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
| N |
 |
| ++ |
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| DB |
 |
| + |
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| DC |
 |
| ++ |
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| DM |
 |
| + |
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| VM |
 |
| |
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| VB |
 |
| |
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