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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6502R-4 |
| Symbol |
E(z) |
Full Name |
Enhancer of zeste |
| CG No |
CG6502 |
Old CG No |
CG6502 |
| Synonyms |
CG6502, E(Z), EZ, E(z)1, Ez, E[z], Su(z)301, pco, l(3)67Fa, l(3)1902, pco/E(z), l(3)ds12, l(3)SG17, l(3)B12, E(z) |
| Accession No (Link to NCBI) |
NM_079297.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Lv X, Han Z, Chen H, Yang B, Yang X, Xia Y, Pan C, Fu L, Zhang S, Han H, Wu M, Zhou Z, Zhang L, Li L, Wei G, Zhao Y.
A positive role for polycomb in transcriptional regulation via H4K20me1.
Cell Res (2017) 27(4) 594
[
PubMed ID = 28386086
]
[
RRC reference
]
Lv X, Han Z, Chen H, Yang B, Yang X, Xia Y, Pan C, Fu L, Zhang S, Han H, Wu M, Zhou Z, Zhang L, Li L, Wei G, Zhao Y.
A positive role for polycomb in transcriptional regulation via H4K20me1.
Cell Res (2016) 26(5) 529-42
[
PubMed ID = 27002220
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAAAAGGTGCCCATTTGCGTGA |
| Primer Seq. 3' |
aatctagaggtaccTTCTTCCACATCGGGCTTAAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AAAAGGTGCC CATTTGCGTG ATCAACGcGG TCACTCCCAT ACCCACCATG TACACGTGGG
0061 CACCAACACA GCAGAACTTC ATGGTGGAGG ATGAGACGGT GCTGCACAAT ATACCCTATA
0121 TGGGCGACGA GGTCCTCGAT AAGGATGGCA AGTTCATAGA GGAACTGATC AAAAACTACG
0181 ATGGCAAGGT CCACGGCGAC AAGGATCCCT CGTTCATGGA CGATGCCATC TTCGTAGAAC
0241 TGGTGCACGC CCTCATGCGC TCCTATAGCA AGGAGCTGGA GGAAGCCGCT CCGAGTACTT
0301 CGACTGCCAT TAAAACANAG ACGTTGGCGA AATCAAAACA GGGCGAAGAT GATGGGGTAG
0361 TTGATGTTGA CGCAGATGGT GAATCTCCCA TGAAGCTTGA GAAAACCGAC AGCAAAGGGG
0421 ATCTGNCTGA AGTTGAGAAG AAGGAAACTG AAGAACCGCT GGAGACTGAA GATGCGGATG
0481 TTAAGCCCGA TGTGGAAGAA |
| in silico PCR Fragment |
0001 AAAAGGTGCC CATTTGCGTG ATCAACGCGG TCACTCCCAT ACCCACCATG TACACGTGGG
0061 CACCAACACA GCAGAACTTC ATGGTGGAGG ATGAGACGGT GCTGCACAAT ATACCCTATA
0121 TGGGCGACGA GGTCCTCGAT AAGGATGGCA AGTTCATAGA GGAACTGATC AAAAACTACG
0181 ATGGCAAGGT TCACGGCGAC AAGGATCCCT CGTTCATGGA CGATGCCATC TTCGTAGAAC
0241 TGGTGCACGC CCTTATGCGC TCCTACAGCA AGGAACTGGA GGAAGCCGCT CCGGGTACAG
0301 CGACTGCCAT TAAAACAGAG ACGTTGGCGA AATCAAAACA GGGCGAAGAT GATGGGGTAG
0361 TTGATGTTGA CGCAGATGGT GAATCTCCCA TGAAGCTTGA GAAAACCGAC AGCAAAGGGG
0421 ATCTGACTGA AGTTGAGAAG AAGGAAACTG AAGAACCGCT GGAGACTGAA GATGCGGATG
0481 TTAAGCCCGA TGTGGAAGAA |
| Assemble Data |
6502R-4.IR_full 1 AAAAGGTGCCCATTTGCGTGATCAACGCGGTCACTCCCATACCCACCATGTACACGTGGG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6502R-4.in silico 1 AAAAGGTGCCCATTTGCGTGATCAACGCGGTCACTCCCATACCCACCATGTACACGTGGG 60
6502R-4.IR_full 61 CACCAACACAGCAGAACTTCATGGTGGAGGATGAGACGGTGCTGCACAATATACCCTATA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6502R-4.in silico 61 CACCAACACAGCAGAACTTCATGGTGGAGGATGAGACGGTGCTGCACAATATACCCTATA 120
6502R-4.IR_full 121 TGGGCGACGAGGTCCTCGATAAGGATGGCAAGTTCATAGAGGAACTGATCAAAAACTACG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6502R-4.in silico 121 TGGGCGACGAGGTCCTCGATAAGGATGGCAAGTTCATAGAGGAACTGATCAAAAACTACG 180
6502R-4.IR_full 181 ATGGCAAGGTCCACGGCGACAAGGATCCCTCGTTCATGGACGATGCCATCTTCGTAGAAC 240
|||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
6502R-4.in silico 181 ATGGCAAGGTTCACGGCGACAAGGATCCCTCGTTCATGGACGATGCCATCTTCGTAGAAC 240
6502R-4.IR_full 241 TGGTGCACGCCCTCATGCGCTCCTATAGCAAGGAGCTGGAGGAAGCCGCTCCGAGTACTT 300
||||||||||||| ||||||||||| |||||||| |||||||||||||||||| ||||
6502R-4.in silico 241 TGGTGCACGCCCTTATGCGCTCCTACAGCAAGGAACTGGAGGAAGCCGCTCCGGGTACAG 300
6502R-4.IR_full 301 CGACTGCCATTAAAACANAGACGTTGGCGAAATCAAAACAGGGCGAAGATGATGGGGTAG 360
||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
6502R-4.in silico 301 CGACTGCCATTAAAACAGAGACGTTGGCGAAATCAAAACAGGGCGAAGATGATGGGGTAG 360
6502R-4.IR_full 361 TTGATGTTGACGCAGATGGTGAATCTCCCATGAAGCTTGAGAAAACCGACAGCAAAGGGG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6502R-4.in silico 361 TTGATGTTGACGCAGATGGTGAATCTCCCATGAAGCTTGAGAAAACCGACAGCAAAGGGG 420
6502R-4.IR_full 421 ATCTGNCTGAAGTTGAGAAGAAGGAAACTGAAGAACCGCTGGAGACTGAAGATGCGGATG 480
||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
6502R-4.in silico 421 ATCTGACTGAAGTTGAGAAGAAGGAAACTGAAGAACCGCTGGAGACTGAAGATGCGGATG 480
6502R-4.IR_full 481 TTAAGCCCGATGTGGAAGAA 500
||||||||||||||||||||
6502R-4.in silico 481 TTAAGCCCGATGTGGAAGAA 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079297.2 |
CG6502-RA (E(z)), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_133138.1 |
CG7884-RA (CG7884), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_132267.4 |
CG12109-RB (Caf1-180), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_165490.1 |
CG3427-RA (Epac), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_169450.1 |
CG14741-RA (CG14741), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_079919.2 |
CG5700-RB (prc), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_166186.2 |
CG18471-RA (gprs), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_139582.1 |
CG12766-RA (CG12766), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_143367.2 |
CG10011-RA (CG10011), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_166300.1 |
CG5473-RB, transcript variant B (SP2637), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_137511.2 |
CG5473-RA, transcript variant A (SP2637), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_139572.1 |
CG10855-RA (CG10855), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_132320.2 |
CG12124-RA (CG12124), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_078559.2 |
CG18085-RA (sev), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079663.2 |
CG5826-RA (Prx5037), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_136002.1 |
CG15142-RA (CG15142), mRNA |
| 0 |
0 |
0 |
11 |
42 |
NM_001032052.1 |
CG33715-RE, transcript variant E (Msp-300), mRNA |
| 0 |
0 |
0 |
6 |
11 |
NM_169909.1 |
CG5460-RB, transcript variant B (H), mRNA |
| 0 |
0 |
0 |
6 |
11 |
NM_169908.1 |
CG5460-RD, transcript variant D (H), mRNA |
| 0 |
0 |
0 |
6 |
11 |
NM_169907.1 |
CG5460-RA, transcript variant A (H), mRNA |
| 0 |
0 |
0 |
6 |
11 |
NM_079694.2 |
CG5460-RC, transcript variant C (H), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_166331.1 |
CG15112-RB, transcript variant B (ena), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_132660.2 |
CG1716-RA (CG1716), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_141444.1 |
CG10061-RA (l(3)s2214), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_058111.3 |
CG13388-RB, transcript variant B (Akap200), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_164820.1 |
CG13388-RC, transcript variant C (Akap200), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_175984.1 |
CG13388-RD, transcript variant D (Akap200), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_058112.3 |
CG13388-RA, transcript variant A (Akap200), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_169913.1 |
CG3856-RA, transcript variant A (Oamb), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_169914.1 |
CG3856-RC, transcript variant C (Oamb), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
 |
|
JNK signal
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Caspase-3 signal
| N |
 |
| ++ |
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| DB |
 |
| + |
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| DC |
 |
| + |
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| DM |
 |
| + |
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| VM |
 |
| |
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| VB |
 |
| |
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