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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 6500R-2 
 Symbol Bx  Full Name Beadex 
 CG No CG6500  Old CG No CG6500 
 Synonyms Lmo, hdp, dlmo, DLMO, dLMO, CG6500, Bd, beadex/dLMO, dttg, Dlmo, mgt, hld, fliH, mgg, hdp-a, Rho, Ptd, Bx, dLmo 
 Accession No (Link to NCBI) NM_167625.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTTTCCTCCAACCAACAGCAGC 
 Primer Seq. 3' 
aatctagaggtaccGAAGCACTCCAAGTGATAGAC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TTTCCTCCAA CCAACAGCAG CAGCAGCAGC TAGCAGTCGC AGCAAAGCAA CGCCGGCATG 
0061 AACAGCGGTG GCCAGAACAA CGGGCCCAAT CCCAATGGCA ATGGGAACGT GGTCAATGTG 
0121 GTCAACTCCG GCGGTGCCgG TGgcGGCAAC AATGGCAACG GGAATGTCCA GAGCATCGCC 
0181 GCCGCTGCCA ACATTAATAA CAATAACAAC AACAACGGCA GCCAATTTGT GCGCCGGCTG 
0241 CGGCAAGCAC ATCCAAGGGA CCGCTATCTC CTCCGCGCCC TGGACATGCT GTGGCACGAG 
0301 GACTGCCTCA AGTGCGGCTG CTGCGACTGc CGCCTGGGCG AGGTgGGCTC CACGCTCTAC 
0361 ACCAAGGGCA ACCTGATgCT CTGCAAGCGG GACTACTTGA GATTGTTTGG CAACACgGGC 
0421 TACTGCGCCG CCTGCAGCAA GGTGATCCCA GCCTTCGAGA TGGTGATGCG TGCAAGGACC 
0481 AATGTCTATC ACTTGGAGTG CTTC 
 in silico PCR Fragment 
0001 TTTCCTCCAA CCAACAGCAG CAGCAGCAGC -AGCAGTCGC AGCAAAGCAA CGCCGGCATG 
0061 AACAGCGGTG GCCAGAACAA CGGGCCCAAT CCCAATGGCA ATGGGAACGT GGTCAATGTG 
0121 GTCAACTCCG GCGGTGCCGG TGGCGGCAAC AATGGCAACG GGAATGTCCA GAGCATCGCC 
0181 GCCGCTGCCA ACAATAATAA CAATAACAAC AACAACGGCA GCCAA-TTGT GCGCCGGCTG 
0241 CGGCAAGCAC ATCCA--GGA CCGCTATCTC CTCCGCGCCC TGGACATGCT GTGGCACGAG 
0301 GACTGCCTCA AGTGCGGCTG CTGCGACTGC CGCCTGGGCG AGGTGGGCTC CACGCTCTAC 
0361 ACCAAGGGCA ACCTGATGCT CTGCAAGCGG GACTACTTGA GATTGTTTGG CAACACGGGC 
0421 TACTGCGCCG CCTGCAGCAA GGTGATCCCA GCCTTCGAGA TGGTGATGCG TGCAAGGACC 
0481 AATGTCTATC ACTTGGAGTG CTTC 
 Assemble Data 

6500R-2.IR_full       1   TTTCCTCCAACCAACAGCAGCAGCAGCAGCTAGCAGTCGCAGCAAAGCAACGCCGGCATG 60
                          |||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
6500R-2.in silico     1   TTTCCTCCAACCAACAGCAGCAGCAGCAGC-AGCAGTCGCAGCAAAGCAACGCCGGCATG 60


6500R-2.IR_full       61  AACAGCGGTGGCCAGAACAACGGGCCCAATCCCAATGGCAATGGGAACGTGGTCAATGTG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6500R-2.in silico     61  AACAGCGGTGGCCAGAACAACGGGCCCAATCCCAATGGCAATGGGAACGTGGTCAATGTG 120


6500R-2.IR_full       121 GTCAACTCCGGCGGTGCCGGTGGCGGCAACAATGGCAACGGGAATGTCCAGAGCATCGCC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6500R-2.in silico     121 GTCAACTCCGGCGGTGCCGGTGGCGGCAACAATGGCAACGGGAATGTCCAGAGCATCGCC 180


6500R-2.IR_full       181 GCCGCTGCCAACATTAATAACAATAACAACAACAACGGCAGCCAATTTGTGCGCCGGCTG 240
                          ||||||||||||| ||||||||||||||||||||||||||||||| ||||||||||||||
6500R-2.in silico     181 GCCGCTGCCAACAATAATAACAATAACAACAACAACGGCAGCCAA-TTGTGCGCCGGCTG 240


6500R-2.IR_full       241 CGGCAAGCACATCCAAGGGACCGCTATCTCCTCCGCGCCCTGGACATGCTGTGGCACGAG 300
                          |||||||||||||||  |||||||||||||||||||||||||||||||||||||||||||
6500R-2.in silico     241 CGGCAAGCACATCCA--GGACCGCTATCTCCTCCGCGCCCTGGACATGCTGTGGCACGAG 300


6500R-2.IR_full       301 GACTGCCTCAAGTGCGGCTGCTGCGACTGCCGCCTGGGCGAGGTGGGCTCCACGCTCTAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6500R-2.in silico     301 GACTGCCTCAAGTGCGGCTGCTGCGACTGCCGCCTGGGCGAGGTGGGCTCCACGCTCTAC 360


6500R-2.IR_full       361 ACCAAGGGCAACCTGATGCTCTGCAAGCGGGACTACTTGAGATTGTTTGGCAACACGGGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6500R-2.in silico     361 ACCAAGGGCAACCTGATGCTCTGCAAGCGGGACTACTTGAGATTGTTTGGCAACACGGGC 420


6500R-2.IR_full       421 TACTGCGCCGCCTGCAGCAAGGTGATCCCAGCCTTCGAGATGGTGATGCGTGCAAGGACC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6500R-2.in silico     421 TACTGCGCCGCCTGCAGCAAGGTGATCCCAGCCTTCGAGATGGTGATGCGTGCAAGGACC 480


6500R-2.IR_full       481 AATGTCTATCACTTGGAGTGCTTC 504
                          ||||||||||||||||||||||||
6500R-2.in silico     481 AATGTCTATCACTTGGAGTGCTTC 504



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  15  54  170  NM_167625.1  CG6500-RA, transcript variant A (Bx), mRNA 
100   482  15  54  170  NM_176758.1  CG6500-RC, transcript variant C (Bx), mRNA 
100   482  15  47  139  NM_078678.2  CG6500-RB, transcript variant B (Bx), mRNA 
25.51   123  404  950  1475  NM_080536.2  CG4013-RA, transcript variant A (Smr), mRNA 
25.51   123  404  950  1475  NM_167334.1  CG4013-RB, transcript variant B (Smr), mRNA 
25.51   123  404  950  1475  NM_167335.1  CG4013-RC, transcript variant C (Smr), mRNA 
8.29   40  108  290  518  NM_131975.3  CG32767-RA, transcript variant A (CG32767), mRNA 
8.29   40  108  290  518  NM_206628.1  CG32767-RB, transcript variant B (CG32767), mRNA 
7.26   35  155  437  756  NM_080376.1  CG8118-RA, transcript variant A (mam), mRNA 
7.26   35  155  437  754  NM_166030.1  CG8118-RB, transcript variant B (mam), mRNA 
6.63   32  107  276  575  NM_079671.2  CG7847-RA, transcript variant A (sr), mRNA 
6.63   32  97  229  466  NM_169786.1  CG7847-RB, transcript variant B (sr), mRNA 
6.01   29  91  201  306  NM_001014565.1  CG10491-RB, transcript variant B (vn), mRNA 
6.01   29  91  201  304  NM_079218.2  CG10491-RA, transcript variant A (vn), mRNA 
6.01   29  47  98  58  NM_144133.1  CG13235-RA (CG13235), mRNA 
5.8   28  50  111  90  NM_144114.3  CG14494-RA (CG14494), mRNA 
5.6   27  123  360  708  NM_001038734.1  CG16902-RC (Hr4), mRNA 
5.6   27  56  113  148  NM_057678.3  CG31240-RA (repo), mRNA 
5.18   25  68  181  262  NM_001038965.1  CG9924-RE, transcript variant E (CG9924), mRNA 
5.18   25  57  150  256  NM_167239.2  CG32677-RA (CG32677), mRNA 
4.97   24  85  220  350  NM_134474.4  CG32532-RA (CG32532), mRNA 
4.97   24  64  157  221  NM_132172.2  CG15478-RA (CG15478), mRNA 
4.77   23  41  88  79  NM_136061.1  CG15166-RA (CG15166), mRNA 
4.56   22  77  273  647  NM_168571.2  CG32133-RA (CG32133), mRNA 
4.35   21  58  144  233  NM_169657.1  CG5166-RB, transcript variant B (Atx2), mRNA 
4.35   21  58  137  221  NM_169658.2  CG5166-RC, transcript variant C (Atx2), mRNA 
4.35   21  58  137  221  NM_142209.2  CG5166-RA, transcript variant A (Atx2), mRNA 
4.35   21  42  76  60  NM_132301.3  CG7065-RA (CG7065), mRNA 
4.14   20  74  197  419  NM_176591.1  CG15532-RC, transcript variant C (hdc), mRNA 
4.14   20  74  197  419  NM_079853.2  CG15532-RA, transcript variant A (hdc), mRNA 

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