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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6474R-2 |
| Symbol |
e(y)1 |
Full Name |
enhancer of yellow 1 |
| CG No |
CG6474 |
Old CG No |
CG6474 |
| Synonyms |
TAF[[II]]40, dmTAF9, Taf9, dmTaf9, dTAF[[II]]40, TAF40/42, dTAF9, TFIID, taf40/e(y)1, TAF[[II]], TAF[II]40, TAF9, dTAFII42, dTAF42, TAFII40, dTAF[[II]]42, d40, TAF40/42/e(y)1, CG6474, TFIID 42, TAFII40(42), TAF[[II]]40/42, Taf40, TAF42, TAF[[II]]42, dTAF[[40]], TAF40, dme-TAFII40, Taf42, Taf[[II]]40, p42, e(y)[1], e(y)4, TFIID-p42, e(y)1 |
| Accession No (Link to NCBI) |
NM_078667.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGAGCGCAGAGAAGTCCGAT |
| Primer Seq. 3' |
aatctagaggtaccATTGGCGCTGGAGACGGGCTT |
| Predicted Fragment Size |
495 |
| IR fragment full Seq |
0001 ATGAGCGCAG AGAAGTCCGA TAAGGCCAAG ATCAGTGCCC AAATCAAGCA CGTGCCGAAG
0061 GACGCGCAGG TGATCATGTC CATCCTGAAG GAGCTGAATG TCCAGGAGTA CGAGCCGCGC
0121 GTNGGTCAAC CAACTGCTGG AGTTCACCTT CCGCTATGTC ACCTGCATTC TGGACGACGC
0181 CAAGGTATAC GCCAACCATG CGCGCAAGAA GACCATCGAC TTGGACGACG TGCGTCTGGC
0241 CACCGAGGTT ACGCTGGACA AGAGCTTCAC CGGGCCGTTG GAGCGCCACG TTCTAGCCAA
0301 GGTGGCCGAC GTGCGCAACA GCATGCCCCT GCCACCCATT AAGCCGCACT GcGGTCTCCN
0361 GACTGCCGCC CGACCGCTAC TGTCTCACCG GCGTCAACTA CAAACTGCGG GCCACTAATC
0421 AGCCCAAGAA AATGACCAAG TCGGCGGTGG AGGGCCGTCC ACTGAAGACC GTCGTTAAGC
0481 CCGTCTCCAG CGCCAAT |
| in silico PCR Fragment |
0001 ATGAGCGCAG AGAAGTCCGA TAAGGCCAAG ATCAGTGCCC AAATCAAGCA CGTGCCGAAG
0061 GACGCGCAGG TGATCATGTC CATCCTGAAG GAGCTGAATG TCCAGGAGTA CGAGCCGCGC
0121 GT-GGTCAAC CAACTGCTGG AGTTCACCTT CCGCTATGTA ACCTGCATTC TGGACGACGC
0181 CAAGGTGTAC GCCAACCATG CGCGCAAGAA GACCATCGAC TTGGACGACG TGCGTCTGGC
0241 CACCGAGGTT ACGCTGGACA AGAGCTTCAC CGGGCCGTTG GAGCGCCACG TTCTAGCCAA
0301 GGTGGCCGAC GTGCGCAACA GCATGCCCCT GCCACCCATT AAGCCGCACT GCGGTCTCC-
0361 GACTGCCGCC CGACCGCTAC TGTCTCACCG GCGTCAACTA CAAACTGCGG GCCACTAATC
0421 AGCCCAAGAA AATGACCAAG TCGGCGGTGG AGGGCCGTCC ACTGAAGACC GTCGTTAAGC
0481 CCGTCTCCAG CGCCAAT |
| Assemble Data |
6474R-2.IR_full 1 ATGAGCGCAGAGAAGTCCGATAAGGCCAAGATCAGTGCCCAAATCAAGCACGTGCCGAAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6474R-2.in silico 1 ATGAGCGCAGAGAAGTCCGATAAGGCCAAGATCAGTGCCCAAATCAAGCACGTGCCGAAG 60
6474R-2.IR_full 61 GACGCGCAGGTGATCATGTCCATCCTGAAGGAGCTGAATGTCCAGGAGTACGAGCCGCGC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6474R-2.in silico 61 GACGCGCAGGTGATCATGTCCATCCTGAAGGAGCTGAATGTCCAGGAGTACGAGCCGCGC 120
6474R-2.IR_full 121 GTNGGTCAACCAACTGCTGGAGTTCACCTTCCGCTATGTCACCTGCATTCTGGACGACGC 180
|| |||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
6474R-2.in silico 121 GT-GGTCAACCAACTGCTGGAGTTCACCTTCCGCTATGTAACCTGCATTCTGGACGACGC 180
6474R-2.IR_full 181 CAAGGTATACGCCAACCATGCGCGCAAGAAGACCATCGACTTGGACGACGTGCGTCTGGC 240
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
6474R-2.in silico 181 CAAGGTGTACGCCAACCATGCGCGCAAGAAGACCATCGACTTGGACGACGTGCGTCTGGC 240
6474R-2.IR_full 241 CACCGAGGTTACGCTGGACAAGAGCTTCACCGGGCCGTTGGAGCGCCACGTTCTAGCCAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6474R-2.in silico 241 CACCGAGGTTACGCTGGACAAGAGCTTCACCGGGCCGTTGGAGCGCCACGTTCTAGCCAA 300
6474R-2.IR_full 301 GGTGGCCGACGTGCGCAACAGCATGCCCCTGCCACCCATTAAGCCGCACTGCGGTCTCCN 360
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6474R-2.in silico 301 GGTGGCCGACGTGCGCAACAGCATGCCCCTGCCACCCATTAAGCCGCACTGCGGTCTCC- 360
6474R-2.IR_full 361 GACTGCCGCCCGACCGCTACTGTCTCACCGGCGTCAACTACAAACTGCGGGCCACTAATC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6474R-2.in silico 361 GACTGCCGCCCGACCGCTACTGTCTCACCGGCGTCAACTACAAACTGCGGGCCACTAATC 420
6474R-2.IR_full 421 AGCCCAAGAAAATGACCAAGTCGGCGGTGGAGGGCCGTCCACTGAAGACCGTCGTTAAGC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6474R-2.in silico 421 AGCCCAAGAAAATGACCAAGTCGGCGGTGGAGGGCCGTCCACTGAAGACCGTCGTTAAGC 480
6474R-2.IR_full 481 CCGTCTCCAGCGCCAAT 497
|||||||||||||||||
6474R-2.in silico 481 CCGTCTCCAGCGCCAAT 497
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
477 |
0 |
0 |
0 |
NM_078667.2 |
CG6474-RA (e(y)1), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_135384.2 |
CG13400-RA (D12), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_165493.1 |
CG15236-RB, transcript variant B (CG15236), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_136397.1 |
CG15236-RA, transcript variant A (CG15236), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_132375.1 |
CG2972-RA (CG2972), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_140010.1 |
CG13313-RA (CG13313), mRNA |
| 0 |
0 |
1 |
4 |
23 |
NM_141666.2 |
CG9492-RA (CG9492), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_130658.2 |
CG10260-RB (CG10260), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_135071.2 |
CG6514-RA (TpnC25D), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_139511.2 |
CG1869-RA (CG1869), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_136949.1 |
CG13151-RA (CG13151), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_143476.2 |
CG7802-RA, transcript variant A (CG7802), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_206583.1 |
CG7802-RB, transcript variant B (CG7802), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_176328.1 |
CG17364-RB, transcript variant B (CG17364), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140417.2 |
CG17364-RA, transcript variant A (CG17364), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_143091.1 |
CG13653-RA (CG13653), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_138110.2 |
CG3683-RA, transcript variant A (CG3683), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166680.1 |
CG3683-RB, transcript variant B (CG3683), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166681.1 |
CG3683-RC, transcript variant C (CG3683), mRNA |
| 0 |
0 |
1 |
2 |
1 |
NM_170644.1 |
CG32464-RA, transcript variant A (l(3)82Fd), mRNA |
| 0 |
0 |
1 |
2 |
1 |
NM_169045.1 |
CG32464-RH, transcript variant H (l(3)82Fd), mRNA |
| 0 |
0 |
1 |
2 |
1 |
NM_170643.1 |
CG32464-RC, transcript variant C (l(3)82Fd), mRNA |
| 0 |
0 |
1 |
2 |
1 |
NM_170642.1 |
CG32464-RI, transcript variant I (l(3)82Fd), mRNA |
| 0 |
0 |
1 |
2 |
1 |
NM_170641.1 |
CG32464-RF, transcript variant F (l(3)82Fd), mRNA |
| 0 |
0 |
1 |
2 |
1 |
NM_001043214.1 |
CG32464-RO, transcript variant O (l(3)82Fd), mRNA |
| 0 |
0 |
1 |
2 |
1 |
NM_170640.2 |
CG32464-RK, transcript variant K (l(3)82Fd), mRNA |
| 0 |
0 |
1 |
2 |
1 |
NM_206429.1 |
CG32464-RG, transcript variant G (l(3)82Fd), mRNA |
| 0 |
0 |
1 |
2 |
1 |
NM_001031978.1 |
CG32464-RM, transcript variant M (l(3)82Fd), mRNA |
| 0 |
0 |
1 |
2 |
1 |
NM_169039.2 |
CG32464-RL, transcript variant L (l(3)82Fd), mRNA |
| 0 |
0 |
1 |
2 |
1 |
NM_143760.2 |
CG32464-RB, transcript variant B (l(3)82Fd), mRNA |
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