NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 6470R-1

Symbol CG6470 Full Name CG6470

CG No CG6470 Old CG No CG6470

Synonyms anon-WO0140519.137, CG6470

Accession No (Link to NCBI) NM_133076.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGACCGGATGCTCCAGGATTC

Primer Seq. 3'

aatctagaggtaccATACTCCCCGCCATCTCCTTC

Predicted Fragment Size

IR fragment full Seq

0001 GGACCGGATG CTCCAGGATT CAGCCGCAGG AATTCGTGGA GGCTCAGGTG GAGAACGCGC 
0061 GCAGGGCGGT GGAGCGCAAG CTGATCTACC TGTCCGAGTC GAACAGAGCC AGTGCATCCA 
0121 CTCGGGCGGC CAAGCGAAAG CACGGCTGGC GCAGGCGGAG GAGCAGGGCG NGGAAGGATC 
0181 GAGCATGGCT GGCCATGCCC ATGGTGAGCG TCGCAGGCGC AGCAAGAGGA GTCTGCTACA 
0241 CGCTCCAGCT CAGGAAGAGA CCGACGAGGA GGAGAGTTCG CCGCTGGAGA AGACGAACAA 
0301 CTACGATAAC AGAAGACGCA GCAGTTGCAA TCTATTGCTA TCTCCTCGTC GCAATGGCCT 
0361 CACGCCCCGG AACTACGAgg ccgaGGCCGA GGCAGCGGAA ATGGAAGCCA TAATGGCCGC 
0421 ACAGCAAGCC TCCAACGGAG CGACGAAGCC CGATCATGAT GAAGGAGATG GCGGGGAGTA 
0481 T

in silico PCR Fragment

0001 GGACCGGATG CTCCAGGATT CAGCCGCAGG AATTCGTGGA GGCTCAGGTG GAGAACGCGC 
0061 GCAGGGCGGT GGAGCGCAAG CTGATCTACC TGTCCGAGTC GAACAGAGCC AGTGCATCCA 
0121 CTCGGGCGGC CAAGCG-AAG CACGGCTGGC GCAGGCGGAG GAGCAGGGCG -GGAAGGATC 
0181 GAGCATGGCT GGCCATGCCC ATGGTGAGCG TCGCAGGCGC AGCAAGAGGA GTCTGCTACA 
0241 CGCTCCAGCT CAGGAAGAGA CCGACGAGGA GGAGAGTTCG CCGCTGGAGA AGACGAACAA 
0301 CTACGATAAC AGAAGACGCA GCAGTTGCAA TCTATTGCTA TCTCCTCGTC GCAATGGCCT 
0361 CACGCCCCGG AACTACGAGG CCGAGGCCGA GGCAGCGGAA ATGGAAGCCA TTATGGCCGC 
0421 ACAGCAAGCC TCCAACGGAA CGACGAAGCC CGATCATGAT GAAGGAGATG GCGGGGAGTA 
0481 T

Assemble Data


6470R-1.IR_full       1   GGACCGGATGCTCCAGGATTCAGCCGCAGGAATTCGTGGAGGCTCAGGTGGAGAACGCGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6470R-1.in silico     1   GGACCGGATGCTCCAGGATTCAGCCGCAGGAATTCGTGGAGGCTCAGGTGGAGAACGCGC 60


6470R-1.IR_full       61  GCAGGGCGGTGGAGCGCAAGCTGATCTACCTGTCCGAGTCGAACAGAGCCAGTGCATCCA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6470R-1.in silico     61  GCAGGGCGGTGGAGCGCAAGCTGATCTACCTGTCCGAGTCGAACAGAGCCAGTGCATCCA 120


6470R-1.IR_full       121 CTCGGGCGGCCAAGCGAAAGCACGGCTGGCGCAGGCGGAGGAGCAGGGCGNGGAAGGATC 180
                          |||||||||||||||| ||||||||||||||||||||||||||||||||| |||||||||
6470R-1.in silico     121 CTCGGGCGGCCAAGCG-AAGCACGGCTGGCGCAGGCGGAGGAGCAGGGCG-GGAAGGATC 180


6470R-1.IR_full       181 GAGCATGGCTGGCCATGCCCATGGTGAGCGTCGCAGGCGCAGCAAGAGGAGTCTGCTACA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6470R-1.in silico     181 GAGCATGGCTGGCCATGCCCATGGTGAGCGTCGCAGGCGCAGCAAGAGGAGTCTGCTACA 240


6470R-1.IR_full       241 CGCTCCAGCTCAGGAAGAGACCGACGAGGAGGAGAGTTCGCCGCTGGAGAAGACGAACAA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6470R-1.in silico     241 CGCTCCAGCTCAGGAAGAGACCGACGAGGAGGAGAGTTCGCCGCTGGAGAAGACGAACAA 300


6470R-1.IR_full       301 CTACGATAACAGAAGACGCAGCAGTTGCAATCTATTGCTATCTCCTCGTCGCAATGGCCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6470R-1.in silico     301 CTACGATAACAGAAGACGCAGCAGTTGCAATCTATTGCTATCTCCTCGTCGCAATGGCCT 360


6470R-1.IR_full       361 CACGCCCCGGAACTACGAGGCCGAGGCCGAGGCAGCGGAAATGGAAGCCATAATGGCCGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
6470R-1.in silico     361 CACGCCCCGGAACTACGAGGCCGAGGCCGAGGCAGCGGAAATGGAAGCCATTATGGCCGC 420


6470R-1.IR_full       421 ACAGCAAGCCTCCAACGGAGCGACGAAGCCCGATCATGATGAAGGAGATGGCGGGGAGTA 480
                          ||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
6470R-1.in silico     421 ACAGCAAGCCTCCAACGGAACGACGAAGCCCGATCATGATGAAGGAGATGGCGGGGAGTA 480


6470R-1.IR_full       481 T 481
                          |
6470R-1.in silico     481 T 481

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	461	0	0	2	NM_133076.2	CG6470-RA (CG6470), mRNA
0	0	4	6	17	NM_176556.1	CG33106-RA, transcript variant A (mask), mRNA
0	0	4	6	17	NM_176557.1	CG33106-RB, transcript variant B (mask), mRNA
0	0	3	4	3	NM_134545.2	CG15459-RA (CG15459), mRNA
0	0	2	2	5	NM_140794.2	CG4108-RA (Chmp1), mRNA
0	0	2	2	3	NM_078981.2	CG8967-RA (otk), mRNA
0	0	2	2	2	NM_168402.1	CG32055-RA (CG32055), mRNA
0	0	2	2	2	NM_078859.2	CG5813-RA, transcript variant A (chif), mRNA
0	0	1	6	2	NM_137718.2	CG9480-RA, transcript variant A (Glycogenin), mRNA
0	0	1	6	2	NM_137235.2	CG8399-RA (CG8399), mRNA
0	0	1	5	6	NM_130536.1	CG14625-RB (CG14625), mRNA
0	0	1	4	11	NM_176460.1	CG31374-RC, transcript variant C (CG31374), mRNA
0	0	1	4	11	NM_169390.2	CG31374-RB, transcript variant B (CG31374), mRNA
0	0	1	4	3	NM_136242.2	CG12050-RA (CG12050), mRNA
0	0	1	3	10	NM_166030.1	CG8118-RB, transcript variant B (mam), mRNA
0	0	1	3	10	NM_080376.1	CG8118-RA, transcript variant A (mam), mRNA
0	0	1	3	10	NM_137910.2	CG9877-RA (CG9877), mRNA
0	0	1	3	6	NM_136710.2	CG1381-RA (CG1381), mRNA
0	0	1	3	4	NM_137806.2	CG5465-RA (MED16), mRNA
0	0	1	3	3	NM_131968.2	CG32772-RA (CG32772), mRNA
0	0	1	2	9	NM_132962.1	CG8945-RC (CG8945), mRNA
0	0	1	2	9	NM_206149.1	CG30460-RA, transcript variant A (CG30460), mRNA
0	0	1	2	9	NM_206150.1	CG30460-RB, transcript variant B (CG30460), mRNA
0	0	1	2	9	NM_137342.2	CG30460-RC, transcript variant C (CG30460), mRNA
0	0	1	2	9	NM_130725.2	CG15239-RA (CG15239), mRNA
0	0	1	2	8	NM_137894.2	CG3788-RA (CG3788), mRNA
0	0	1	2	7	NM_206148.1	CG30460-RD, transcript variant D (CG30460), mRNA
0	0	1	2	6	NM_135954.1	CG13260-RA (CG13260), mRNA
0	0	1	2	5	NM_143480.2	CG18041-RA (CG18041), mRNA
0	0	1	2	4	NM_167554.1	CG4949-RB, transcript variant B (CG4949), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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