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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6459R-1 |
| Symbol |
CG6459 |
Full Name |
CG6459 |
| CG No |
CG6459 |
Old CG No |
CG6459 |
| Synonyms |
CG6459 |
| Accession No (Link to NCBI) |
NM_137399.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ibaraki K, Nakatsuka M, Ohsako T, Watanabe M, Miyazaki Y, Shirakami M, Karr TL, Sanuki R, Tomaru M, Takano-Shimizu-Kouno T.
A cross-species approach for the identification of Drosophila male sterility genes.
G3 (Bethesda) (2021) 11(8)
[
PubMed ID = 34849808
]
[
RRC reference
]
Imai Y, Meng H, Shiba-Fukushima K, Hattori N.
Twin CHCH Proteins, CHCHD2, and CHCHD10: Key Molecules of Parkinson's Disease, Amyotrophic Lateral Sclerosis, and Frontotemporal Dementia.
Int J Mol Sci (2019) 20(4)
[
PubMed ID = 30791515
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGAACAGCCTAAGGACAGCA |
| Primer Seq. 3' |
aatctagaggtaccAGCGTAGAGTTGCCCTTTATG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGAACAGCC TAAGGACAGC AACCATGCGA TTTTGCCGCT CTGGCCAGCT CCAGTGCCGG
0061 ATCTCTCGGC AAGCGCGATT TCACCCGCAG CCTGTGGCAC ATGACCAAAA AGCCGGTGAC
0121 AGCCGGAGGA TCCGATCATG TAACCGTGTT GAATCTCCAC AAGCCCAGCA TTAATTGCAC
0181 GTGCGGCTGC AACGTGCACA CCAAATGTGA GCGGGAACTG GTGGAGTTCC TCACCGAGGA
0241 GATAGTGGCC GAGCGCAAGG TTCAAAAGGG CAAGACGGTG CCAAGCACCC TGGATGGATT
0301 CGCCGTAAAA CTGACCGGCG CCGATGTGGA GCTCACCAAG CAGACGGATA AGGAAAAGGT
0361 GGTGGTCAGC TTCAATGTGA ACCATACGGT GGACAGCGAG GAGGAGCCCG AAATCAACCC
0421 CAATGCTGAC AAGCCGGATC TCGGTGAAAT GAGAAGCAAG CCCCAGTTCG AGGTGGACAT
0481 CATAAAGGGC AACTCTACGC T |
| in silico PCR Fragment |
0001 ATGAACAGCC TAAGGACAGC AACCATGCGA -TTTGCCGCT CTGGCCAGCT CCAGTGCCGG
0061 ATCTCTCGGC AAGCGCGATT TCACCCGCAG CCTGTGGCAC ATGACCAAAA AGCCGGTGAC
0121 AGCCGGAGGA TCCGATCATG TAACCGTGTT GAATCTCCAC AAGCCCAGCA TTAATTGCAC
0181 GTGCGGCTGC AACGTGCACA CCAAATGTGA GCGGGAACTG GTGGAGTTCC TCACCGAGGA
0241 GATAGTGGCC GAGCGCAAGG TTCAAAAGGG CAAGACGGTG CCAAGCACCC TGGATGGATT
0301 CGCCGTAAAA CTGACCGGCG CCGATGTGGA GCTCACCAAG CAGACGGATA AGGAAAAGGT
0361 GGTGGTCAGC TTCAATGTGA ACCATACGGT GGACAGCGAG GAGGAGCCCG AAATCAACCC
0421 CAATGCTGAC AAGCCGGATC TCGGTGAAAT GAGAAGCAAG CCCCAGTTCG AGGTGGACAT
0481 CATAAAGGGC AACTCTACGC T |
| Assemble Data |
6459R-1.IR_full 1 ATGAACAGCCTAAGGACAGCAACCATGCGATTTTGCCGCTCTGGCCAGCTCCAGTGCCGG 60
|||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
6459R-1.in silico 1 ATGAACAGCCTAAGGACAGCAACCATGCGA-TTTGCCGCTCTGGCCAGCTCCAGTGCCGG 60
6459R-1.IR_full 61 ATCTCTCGGCAAGCGCGATTTCACCCGCAGCCTGTGGCACATGACCAAAAAGCCGGTGAC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6459R-1.in silico 61 ATCTCTCGGCAAGCGCGATTTCACCCGCAGCCTGTGGCACATGACCAAAAAGCCGGTGAC 120
6459R-1.IR_full 121 AGCCGGAGGATCCGATCATGTAACCGTGTTGAATCTCCACAAGCCCAGCATTAATTGCAC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6459R-1.in silico 121 AGCCGGAGGATCCGATCATGTAACCGTGTTGAATCTCCACAAGCCCAGCATTAATTGCAC 180
6459R-1.IR_full 181 GTGCGGCTGCAACGTGCACACCAAATGTGAGCGGGAACTGGTGGAGTTCCTCACCGAGGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6459R-1.in silico 181 GTGCGGCTGCAACGTGCACACCAAATGTGAGCGGGAACTGGTGGAGTTCCTCACCGAGGA 240
6459R-1.IR_full 241 GATAGTGGCCGAGCGCAAGGTTCAAAAGGGCAAGACGGTGCCAAGCACCCTGGATGGATT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6459R-1.in silico 241 GATAGTGGCCGAGCGCAAGGTTCAAAAGGGCAAGACGGTGCCAAGCACCCTGGATGGATT 300
6459R-1.IR_full 301 CGCCGTAAAACTGACCGGCGCCGATGTGGAGCTCACCAAGCAGACGGATAAGGAAAAGGT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6459R-1.in silico 301 CGCCGTAAAACTGACCGGCGCCGATGTGGAGCTCACCAAGCAGACGGATAAGGAAAAGGT 360
6459R-1.IR_full 361 GGTGGTCAGCTTCAATGTGAACCATACGGTGGACAGCGAGGAGGAGCCCGAAATCAACCC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6459R-1.in silico 361 GGTGGTCAGCTTCAATGTGAACCATACGGTGGACAGCGAGGAGGAGCCCGAAATCAACCC 420
6459R-1.IR_full 421 CAATGCTGACAAGCCGGATCTCGGTGAAATGAGAAGCAAGCCCCAGTTCGAGGTGGACAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6459R-1.in silico 421 CAATGCTGACAAGCCGGATCTCGGTGAAATGAGAAGCAAGCCCCAGTTCGAGGTGGACAT 480
6459R-1.IR_full 481 CATAAAGGGCAACTCTACGCT 501
|||||||||||||||||||||
6459R-1.in silico 481 CATAAAGGGCAACTCTACGCT 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_137399.2 |
CG6459-RA (CG6459), mRNA |
| 0.62 |
3 |
2 |
3 |
7 |
NM_143605.2 |
CG11339-RA (CG11339), mRNA |
| 0 |
0 |
3 |
6 |
4 |
NM_167047.1 |
CG3125-RB, transcript variant B (l(1)G0060), mRNA |
| 0 |
0 |
3 |
6 |
4 |
NM_132025.2 |
CG3125-RA, transcript variant A (l(1)G0060), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_136175.2 |
CG10728-RA (vls), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_165041.1 |
CG6866-RA, transcript variant A (loqs), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_135802.2 |
CG6866-RB, transcript variant B (loqs), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_001038814.1 |
CG6866-RC, transcript variant C (loqs), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_206459.1 |
CG31284-RC, transcript variant C (CG31284), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_169200.1 |
CG31284-RA, transcript variant A (CG31284), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_169201.1 |
CG31284-RB, transcript variant B (CG31284), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_131946.3 |
CG12184-RA (CG12184), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167011.2 |
CG12179-RA, transcript variant A (CG12179), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_131947.2 |
CG12179-RB, transcript variant B (CG12179), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_057990.3 |
CG2711-RA (dwg), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_135076.2 |
CG14024-RA (CG14024), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_132559.1 |
CG2750-RA (CG2750), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_139980.2 |
CG6662-RA (CG6662), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_138241.2 |
CG9192-RA (CG9192), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_142509.2 |
CG5451-RA (CG5451), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_133018.2 |
CG6492-RA (Ucp4A), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_167686.1 |
CG11940-RB, transcript variant B (CG11940), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_134519.1 |
CG11940-RA, transcript variant A (CG11940), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_140383.1 |
CG10191-RA (CG10191), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_143062.2 |
CG11780-RA (beta4GalT7), mRNA |
| 0 |
0 |
0 |
7 |
3 |
NM_142950.1 |
CG5986-RA (CG5986), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_133103.2 |
CG7280-RA (CG7280), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_057244.3 |
CG10501-RA, transcript variant A (amd), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_165278.1 |
CG10501-RB, transcript variant B (amd), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_132981.1 |
CG8675-RA (CG8675), mRNA |
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