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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 6439R-2 
 Symbol CG6439  Full Name CG6439 
 CG No CG6439  Old CG No CG6439 
 Synonyms FBgn0038922, CG6439 
 Accession No (Link to NCBI) NM_142743.1 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication]
Jeong J, Lee J, Kim JH, Lim C.
Metabolic flux from the Krebs cycle to glutamate transmission tunes a neural brake on seizure onset.
PLoS Genet (2021) 17(10) e1009871 [ PubMed ID = 34714823 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgCGCTACGGGCTACGGATAACT 
 Primer Seq. 3'
aatctagaggtaccGTCGAAGGCGAACTTGGCAAT 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 CGCTACGGGC TACGGATAAC TAcggCGCCA ACAGGACCAC CTGCACACTG ATTCCCGGCG 
0061 ACGGTGTGGG TCCCGAGCTG GTCTACTCCC TCCAGGAGGT CTTCAAGGCA GCCAGCGTTC 
0121 CCGTGGATTT CGAGTGCTAC TTCCTTTCCG AAATCAATCC AGTGCTTAGT GCCAAACTGG 
0181 AGGATGTGGT TGCCTCCATT CAGAAAAACA AAGTGTGCAT TAAGGGCGTT TTGGCCACAC 
0241 CCGACTACAG CAATGTGGGT GACCTGCAGA CCCTTAACAT GAAGCTTCGC AACGACCTGG 
0301 ACCTCTACGC CAATGTGGTG CATGTGCGCA GCTTGCCCGG TGTCAAGACC CGCCACACCA 
0361 ACATCGACAC GGTGATTATT CGCGAGCAGA CGGAGGGCGA GTATTCGGCG TTGGAGCACG 
0421 AGTCGGTGCC CGGCATTGTG GAGTGCCTGA AGATTATCAC CGCCAAAAAG TCCATGCGCA 
0481 TTGCCAAGTT CGCCTTCGAC  
 in silico PCR Fragment
0001 CGCTACGGGC TACGGATAAC TACGGCGCCA ACAGGACCAC CTGCACACTG ATTCCCGGCG 
0061 ACGGTGTGGG TCCCGAGCTG GTCTACTCCC TCCAGGAGGT CTTCAAGGCA GCCAGCGTTC 
0121 CCGTGGACTT CGAGTGCTAC TTCCTTTCCG AAATCAATCC CGTGCTTAGT GCCAAACTGG 
0181 AGGATGTGGT GGCATCCATT CAGAAAAACA AAGTGTGCAT TAAGGGCGTT TTGGCCACAC 
0241 CCGACTACAG CAATGTGGGT GACCTGCAGA CCCTTAACAT GAAGCTTCGC AACGACCTGG 
0301 ACCTCTACGC CAATGTGGTG CATGTGCGCA GCTTGCCCGG TGTCAAGACC CGCCACACCA 
0361 ACATCGACAC GGTGATTATT CGCGAGCAGA CGGAGGGCGA GTATTCGGCG TTGGAGCACG 
0421 AGTCGGTGCC CGGCATTGTG GAGTGCCTGA AGATTATCAC CGCCAAAAAG TCCATGCGCA 
0481 TTGCCAAGTT CGCCTTCGAC  
 Assemble Data

6439R-2.IR_full       1   CGCTACGGGCTACGGATAACTACGGCGCCAACAGGACCACCTGCACACTGATTCCCGGCG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6439R-2.in silico     1   CGCTACGGGCTACGGATAACTACGGCGCCAACAGGACCACCTGCACACTGATTCCCGGCG 60


6439R-2.IR_full       61  ACGGTGTGGGTCCCGAGCTGGTCTACTCCCTCCAGGAGGTCTTCAAGGCAGCCAGCGTTC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6439R-2.in silico     61  ACGGTGTGGGTCCCGAGCTGGTCTACTCCCTCCAGGAGGTCTTCAAGGCAGCCAGCGTTC 120


6439R-2.IR_full       121 CCGTGGATTTCGAGTGCTACTTCCTTTCCGAAATCAATCCAGTGCTTAGTGCCAAACTGG 180
                          ||||||| |||||||||||||||||||||||||||||||| |||||||||||||||||||
6439R-2.in silico     121 CCGTGGACTTCGAGTGCTACTTCCTTTCCGAAATCAATCCCGTGCTTAGTGCCAAACTGG 180


6439R-2.IR_full       181 AGGATGTGGTTGCCTCCATTCAGAAAAACAAAGTGTGCATTAAGGGCGTTTTGGCCACAC 240
                          |||||||||| || ||||||||||||||||||||||||||||||||||||||||||||||
6439R-2.in silico     181 AGGATGTGGTGGCATCCATTCAGAAAAACAAAGTGTGCATTAAGGGCGTTTTGGCCACAC 240


6439R-2.IR_full       241 CCGACTACAGCAATGTGGGTGACCTGCAGACCCTTAACATGAAGCTTCGCAACGACCTGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6439R-2.in silico     241 CCGACTACAGCAATGTGGGTGACCTGCAGACCCTTAACATGAAGCTTCGCAACGACCTGG 300


6439R-2.IR_full       301 ACCTCTACGCCAATGTGGTGCATGTGCGCAGCTTGCCCGGTGTCAAGACCCGCCACACCA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6439R-2.in silico     301 ACCTCTACGCCAATGTGGTGCATGTGCGCAGCTTGCCCGGTGTCAAGACCCGCCACACCA 360


6439R-2.IR_full       361 ACATCGACACGGTGATTATTCGCGAGCAGACGGAGGGCGAGTATTCGGCGTTGGAGCACG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6439R-2.in silico     361 ACATCGACACGGTGATTATTCGCGAGCAGACGGAGGGCGAGTATTCGGCGTTGGAGCACG 420


6439R-2.IR_full       421 AGTCGGTGCCCGGCATTGTGGAGTGCCTGAAGATTATCACCGCCAAAAAGTCCATGCGCA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6439R-2.in silico     421 AGTCGGTGCCCGGCATTGTGGAGTGCCTGAAGATTATCACCGCCAAAAAGTCCATGCGCA 480


6439R-2.IR_full       481 TTGCCAAGTTCGCCTTCGAC 500
                          ||||||||||||||||||||
6439R-2.in silico     481 TTGCCAAGTTCGCCTTCGAC 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_142743.1  CG6439-RA (CG6439), mRNA 
0   NM_137256.1  CG15701-RA (CG15701), mRNA 
0   NM_167346.1  CG4353-RA, transcript variant A (hep), mRNA 
0   NM_078587.2  CG4353-RC, transcript variant C (hep), mRNA 
0   NR_002483.1  CG4353-RC, transcript variant C (hep), mRNA, miscRNA 
0   NM_169874.1  CG4608-RA (bnl), mRNA 
0   NM_078582.3  CG32659-RA (Ten-a), mRNA 
0   NM_138031.1  CG3173-RA (CG3173), mRNA 
0   NM_168115.1  CG10645-RA, transcript variant A (lama), mRNA 
0   NM_079209.2  CG10645-RB, transcript variant B (lama), mRNA 
0   NM_168116.1  CG10645-RC, transcript variant C (lama), mRNA 
0   NM_169487.1  CG31342-RA (CG31342), mRNA 
0   NM_132263.2  CG12111-RA (CG12111), mRNA 
0   NM_168534.2  CG11271-RB, transcript variant B (RpS12), mRNA 
0   NM_079328.2  CG11271-RC, transcript variant C (RpS12), mRNA 
0   NM_136237.2  CG9265-RA (CG9265), mRNA 
0   NM_143359.1  CG14065-RA (CG14065), mRNA 
0   NM_079170.2  CG1066-RB, transcript variant B (Shab), mRNA 
0   NM_001043115.1  CG1066-RC, transcript variant C (Shab), mRNA 
0   NM_167967.1  CG1066-RA, transcript variant A (Shab), mRNA 
0   NM_134317.3  CG12021-RA, transcript variant A (Patj), mRNA 
0   NM_057994.4  CG12021-RC, transcript variant C (Patj), mRNA 
0   NM_167917.2  CG12021-RB, transcript variant B (Patj), mRNA 
0   NM_165168.1  CG31738-RA, transcript variant A (CG31738), mRNA 
0   NM_135957.2  CG31738-RB, transcript variant B (CG31738), mRNA 
0   NM_140068.2  CG3428-RA (CG3428), mRNA 
0   NM_164993.1  CG6618-RA, transcript variant A (Patsas), mRNA 
0   NM_165621.1  CG30357-RA (CG30357), mRNA 
0   NM_165839.2  CG17835-RD, transcript variant D (inv), mRNA 
0   NM_165838.2  CG17835-RC, transcript variant C (inv), mRNA 

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