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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 6418R-2 
 Symbol CG6418  Full Name CG6418 
 CG No CG6418  Old CG No CG6418 
 Synonyms DmRH27, cg6418, CG6418 
 Accession No (Link to NCBI) NM_140156.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Bauke AC, Sasse S, Matzat T, Klämbt C.
A transcriptional network controlling glial development in the Drosophila visual system.
Development (2015) 142(12) 2184-93 [ PubMed ID = 26015542 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTCTGGTTCCCGCGGAGGATTT 
 Primer Seq. 3' 
aatctagaggtaccATGGGATTGCCATCCTCATCG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TCTGGTTCCC GCGGAGGATT TGGAGGAGCA AGCCGTGGCG GTGTCACCGG GAGCACTACA 
0061 TCAACGTCCA AGCACGGCTA CTCCACCTTG GACTCCATCA GCCAGTACAC CAATGCCACC 
0121 AATCTGGTTG GCAAAAGGAA GCACCACACG GACGATGACT ATTTCGATGA TGACGATGAG 
0181 CCCCGGGAAA AGGAGATAGA ACAAGCCTAT ATACCGGCAC CAGGATCCCC GGNAGCTCCG 
0241 CCNAACGTCG GCCAAAAAGC AAGAGGATTC CGATTCGGAT GAGGATCCCC TCGAGCAGTT 
0301 CATGGCGGGC ATCAACCAGC AGGTGGAGAA GGAGAAGCGT CAACAAGCAC CTAAGCCGCC 
0361 AACTCAGGCG GTGAGAGGTG ACATCGATGA CGAGGACGAT GAGGAGAGCT ACTATCGCTA 
0421 CATGAAGGAG AATCCCAATG CTGGACTTCG CGACGAGGGA TCCGATCAGG AAATCGAATA 
0481 CGATGAGGAT GGCAATCCCA T 
 in silico PCR Fragment 
0001 TCTGGTTCCC GCGGAGGATT TGGAGGAGCA AGCCGTGGCG GTGTCACCGG GAGCACTACA 
0061 TCAACGTCCA AGCACGGCTA CTCCACCTTG GACTCCATCA GCCAGTACAC CAATGCCACC 
0121 AATCTGGTTG GCAAAAGGAA GCACCACACG GACGATGACT ATTTCGATGA TGACGATGAG 
0181 CCCCGGGAAA AGGAGATAGA ACAAGCCTAT ATACCGGCAC CAGGATCCCC GGGAGCTCCG 
0241 CC-AACGTCG GCCAAAAAGC AAGAGGATTC CGATTCGGAT GAGGATCCCC TCGAGCAGTT 
0301 CATGGCGGGC ATCAACCAGC AGGTGGAGAA GGAGAAGCGT CAACAAGCAC CTAAGCCGCC 
0361 AACTCAGGCG GTGAGAGGTG ACATCGATGA CGAGGACGAT GAGGAGAGCT ACTATCGCTA 
0421 CATGAAGGAG AATCCCAATG CTGGACTTCG CGACGAGGGA TCCGATCAGG AAATCGAATA 
0481 CGATGAGGAT GGCAATCCCA T 
 Assemble Data 

6418R-2.IR_full       1   TCTGGTTCCCGCGGAGGATTTGGAGGAGCAAGCCGTGGCGGTGTCACCGGGAGCACTACA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6418R-2.in silico     1   TCTGGTTCCCGCGGAGGATTTGGAGGAGCAAGCCGTGGCGGTGTCACCGGGAGCACTACA 60


6418R-2.IR_full       61  TCAACGTCCAAGCACGGCTACTCCACCTTGGACTCCATCAGCCAGTACACCAATGCCACC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6418R-2.in silico     61  TCAACGTCCAAGCACGGCTACTCCACCTTGGACTCCATCAGCCAGTACACCAATGCCACC 120


6418R-2.IR_full       121 AATCTGGTTGGCAAAAGGAAGCACCACACGGACGATGACTATTTCGATGATGACGATGAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6418R-2.in silico     121 AATCTGGTTGGCAAAAGGAAGCACCACACGGACGATGACTATTTCGATGATGACGATGAG 180


6418R-2.IR_full       181 CCCCGGGAAAAGGAGATAGAACAAGCCTATATACCGGCACCAGGATCCCCGGNAGCTCCG 240
                          |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
6418R-2.in silico     181 CCCCGGGAAAAGGAGATAGAACAAGCCTATATACCGGCACCAGGATCCCCGGGAGCTCCG 240


6418R-2.IR_full       241 CCNAACGTCGGCCAAAAAGCAAGAGGATTCCGATTCGGATGAGGATCCCCTCGAGCAGTT 300
                          || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6418R-2.in silico     241 CC-AACGTCGGCCAAAAAGCAAGAGGATTCCGATTCGGATGAGGATCCCCTCGAGCAGTT 300


6418R-2.IR_full       301 CATGGCGGGCATCAACCAGCAGGTGGAGAAGGAGAAGCGTCAACAAGCACCTAAGCCGCC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6418R-2.in silico     301 CATGGCGGGCATCAACCAGCAGGTGGAGAAGGAGAAGCGTCAACAAGCACCTAAGCCGCC 360


6418R-2.IR_full       361 AACTCAGGCGGTGAGAGGTGACATCGATGACGAGGACGATGAGGAGAGCTACTATCGCTA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6418R-2.in silico     361 AACTCAGGCGGTGAGAGGTGACATCGATGACGAGGACGATGAGGAGAGCTACTATCGCTA 420


6418R-2.IR_full       421 CATGAAGGAGAATCCCAATGCTGGACTTCGCGACGAGGGATCCGATCAGGAAATCGAATA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6418R-2.in silico     421 CATGAAGGAGAATCCCAATGCTGGACTTCGCGACGAGGGATCCGATCAGGAAATCGAATA 480


6418R-2.IR_full       481 CGATGAGGATGGCAATCCCAT 501
                          |||||||||||||||||||||
6418R-2.in silico     481 CGATGAGGATGGCAATCCCAT 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_140156.1  CG6418-RB (CG6418), mRNA 
0.41   2  2  2  2  NM_206259.1  CG1271-RC, transcript variant C (CG1271), mRNA 
0.41   2  2  2  2  NM_167974.1  CG1271-RB, transcript variant B (CG1271), mRNA 
0.41   2  2  2  2  NM_139506.2  CG1271-RA, transcript variant A (CG1271), mRNA 
0.41   2  2  2  2  NM_167973.1  CG1271-RD, transcript variant D (CG1271), mRNA 
0.2   1  5  7  4  NM_130685.1  CG14420-RA (CG14420), mRNA 
0   0  4  2  5  NM_132200.1  CG1531-RB (CG1531), mRNA 
0   0  4  2  4  NM_137114.1  CG30070-RA (CG30070), mRNA 
0   0  3  7  41  NM_168400.1  CG6611-RB, transcript variant B (ect), mRNA 
0   0  3  7  41  NM_079967.4  CG6611-RA, transcript variant A (ect), mRNA 
0   0  3  7  38  NM_168401.1  CG6611-RC, transcript variant C (ect), mRNA 
0   0  3  6  27  NM_132529.2  CG2025-RA (CG2025), mRNA 
0   0  3  4  7  NM_135393.2  CG13097-RA (CG13097), mRNA 
0   0  3  3  4  NM_136741.2  CG11763-RC, transcript variant C (micr), mRNA 
0   0  3  3  4  NM_165770.1  CG11763-RA, transcript variant A (micr), mRNA 
0   0  3  3  4  NM_001038849.1  CG11763-RD, transcript variant D (micr), mRNA 
0   0  3  2  6  NM_131979.1  CG5062-RA (CG5062), mRNA 
0   0  2  7  15  NM_176498.1  CG9297-RA, transcript variant A (CG9297), mRNA 
0   0  2  5  2  NM_170278.1  CG31082-RA (CG31082), mRNA 
0   0  2  4  11  NM_176497.1  CG9297-RB, transcript variant B (CG9297), mRNA 
0   0  2  3  13  NM_137047.2  CG6197-RA (CG6197), mRNA 
0   0  2  3  4  NM_206564.1  CG5794-RE, transcript variant E (CG5794), mRNA 
0   0  2  3  4  NM_143018.2  CG5794-RD, transcript variant D (CG5794), mRNA 
0   0  2  3  3  NM_140842.1  CG9629-RA (CG9629), mRNA 
0   0  2  3  3  NM_170158.1  CG5794-RB, transcript variant B (CG5794), mRNA 
0   0  2  3  3  NM_132514.2  CG1837-RA (CG1837), mRNA 
0   0  2  2  24  NM_132831.1  CG8184-RB (CG8184), mRNA 
0   0  2  2  10  NM_141235.1  CG14654-RA (CG14654), mRNA 
0   0  2  2  9  NM_140079.1  CG3280-RA (CG3280), mRNA 
0   0  2  2  7  NM_080049.2  CG11614-RA (nkd), mRNA 

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