NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 6417R-1

Symbol Oatp33Eb Full Name Organic anion transporting polypeptide 33Eb

CG No CG6417 Old CG No CG6417

Synonyms oatp 33Eb, CG6417, Oatp33Eb

Accession No (Link to NCBI) NM_135726.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGCGCATAATACGCGCAAC

Primer Seq. 3'

aatctagaggtaccGTCAGTGTGAGCCTTTGGAAC

Predicted Fragment Size

IR fragment full Seq

0001 ATGGCGCATA ATACGCGCAA CTCCGATTTA TCGCAGATAG CTGTCAATGC CAACAATATA 
0061 CCCGATGACA GCGTCGATTG TGGCATCAAG GGTCTGGGAT GGTGTCGCGG TCCCGGATGC 
0121 CAGAAATATG CCCGACTGCG CACCTTCATC CTGGTACTGG CCATCTCGGG AACCCTACAG 
0181 GGCGCCTGCG AATCCTACTT CCGCGTGTCG GCCAAACAGG CTTCCTTTCA GTTCGGTTGG 
0241 AATCCACTGA TTGTGGATTG GCTGCTGGTG GCCAGTGGAT TATTCCAGGC GGTCTTTGCG 
0301 TTGGCTTTCG CCTACTGGGC GGNACGTCTA TCATCCCATC AAATGGCTGG TGGGAACTCT 
0361 GATGCTGCAG GCGGTCACCT GTGTGGTGGC TGTGATTCCC TCCATTATGA ACTTTGCCGA 
0421 TGGCAACCAG GCAAAGGGAT NCTCTGCTGG ATGCCAATAA TGTGCGCCAC ANTCCTTGGC 
0481 ACAGTTCCAA AGGCTCACAC TGAC

in silico PCR Fragment

0001 ATGGCGCATA ATACGCGCAA CTCCGATTTA TCGCAGATAG CTGTCAATGC CAACAATATA 
0061 CCCGATGACA GCGTCGATTG TGGCATCAAG GGTCTGGGAT GGTGTCGCGG TCCCGGATGC 
0121 CAGAAATATG CCCGACTGCG CACCTTCATC CTGGTACTGG CCATCTCGGG AACCCTACAG 
0181 GGCGCCTGCG AATCCTACTT CCGCGTGTCG GCCAAACAGG CTTCCTTTCA GTTCGGTTGG 
0241 AATCCACTGA TTGTGGATTG GCTGCTGGTG GCCAGTGGAT TATTCCAGGC GGTCTTTGCG 
0301 TTGGCTTTCG CCTACTGGGC GG-ACGTCTA TCATCCCATC AAATGGCTGG TGGGAACTCT 
0361 GATGCTGCAG GCGGTCACCT GTGTGGTGGC TGTGATTCCC TCCATTATGA ACTTTGCCGA 
0421 TGGCAACCAG GCAAA-GGAT GCTCTGCTGG ATGCCAAT-A TGTGCGCCAC A-TCCTTGGC 
0481 ACAGTTCCAA AGGCTCACAC TGAC

Assemble Data


6417R-1.IR_full       1   ATGGCGCATAATACGCGCAACTCCGATTTATCGCAGATAGCTGTCAATGCCAACAATATA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6417R-1.in silico     1   ATGGCGCATAATACGCGCAACTCCGATTTATCGCAGATAGCTGTCAATGCCAACAATATA 60


6417R-1.IR_full       61  CCCGATGACAGCGTCGATTGTGGCATCAAGGGTCTGGGATGGTGTCGCGGTCCCGGATGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6417R-1.in silico     61  CCCGATGACAGCGTCGATTGTGGCATCAAGGGTCTGGGATGGTGTCGCGGTCCCGGATGC 120


6417R-1.IR_full       121 CAGAAATATGCCCGACTGCGCACCTTCATCCTGGTACTGGCCATCTCGGGAACCCTACAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6417R-1.in silico     121 CAGAAATATGCCCGACTGCGCACCTTCATCCTGGTACTGGCCATCTCGGGAACCCTACAG 180


6417R-1.IR_full       181 GGCGCCTGCGAATCCTACTTCCGCGTGTCGGCCAAACAGGCTTCCTTTCAGTTCGGTTGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6417R-1.in silico     181 GGCGCCTGCGAATCCTACTTCCGCGTGTCGGCCAAACAGGCTTCCTTTCAGTTCGGTTGG 240


6417R-1.IR_full       241 AATCCACTGATTGTGGATTGGCTGCTGGTGGCCAGTGGATTATTCCAGGCGGTCTTTGCG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6417R-1.in silico     241 AATCCACTGATTGTGGATTGGCTGCTGGTGGCCAGTGGATTATTCCAGGCGGTCTTTGCG 300


6417R-1.IR_full       301 TTGGCTTTCGCCTACTGGGCGGNACGTCTATCATCCCATCAAATGGCTGGTGGGAACTCT 360
                          |||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
6417R-1.in silico     301 TTGGCTTTCGCCTACTGGGCGG-ACGTCTATCATCCCATCAAATGGCTGGTGGGAACTCT 360


6417R-1.IR_full       361 GATGCTGCAGGCGGTCACCTGTGTGGTGGCTGTGATTCCCTCCATTATGAACTTTGCCGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6417R-1.in silico     361 GATGCTGCAGGCGGTCACCTGTGTGGTGGCTGTGATTCCCTCCATTATGAACTTTGCCGA 420


6417R-1.IR_full       421 TGGCAACCAGGCAAAGGGATNCTCTGCTGGATGCCAATAATGTGCGCCACANTCCTTGGC 480
                          ||||||||||||||| |||| ||||||||||||||||| |||||||||||| ||||||||
6417R-1.in silico     421 TGGCAACCAGGCAAA-GGATGCTCTGCTGGATGCCAAT-ATGTGCGCCACA-TCCTTGGC 480


6417R-1.IR_full       481 ACAGTTCCAAAGGCTCACACTGAC 504
                          ||||||||||||||||||||||||
6417R-1.in silico     481 ACAGTTCCAAAGGCTCACACTGAC 504

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_135726.2	CG6417-RA (Oatp33Eb), mRNA
0	0	2	5	5	NM_079992.3	CG17976-RA (sut3), mRNA
0	0	2	3	5	NM_132622.1	CG18646-RA (CG18646), mRNA
0	0	2	2	3	NM_080342.2	CG1787-RA (Hexo2), mRNA
0	0	2	2	3	NM_167641.1	CG14195-RA (CG14195), mRNA
0	0	1	8	7	NM_165169.3	CG5996-RB, transcript variant B (trpgamma), mRNA
0	0	1	8	7	NM_135958.3	CG5996-RA, transcript variant A (trpgamma), mRNA
0	0	1	7	6	NM_170324.1	CG31064-RB, transcript variant B (CG31064), mRNA
0	0	1	4	4	NM_176567.1	CG33102-RA (Hex-t1), mRNA
0	0	1	4	3	NM_143288.2	CG31064-RE, transcript variant E (CG31064), mRNA
0	0	1	4	3	NM_170325.1	CG31064-RA, transcript variant A (CG31064), mRNA
0	0	1	4	2	NM_142692.1	CG5802-RA (CG5802), mRNA
0	0	1	3	5	NM_078670.2	CG7092-RA (Dhc16F), mRNA
0	0	1	3	3	NM_139595.2	CG1135-RA (CG1135), mRNA
0	0	1	3	3	NM_140184.2	CG7628-RA (CG7628), mRNA
0	0	1	2	5	NM_139367.3	CG7864-RA (CG7864), mRNA
0	0	1	2	4	NM_143367.2	CG10011-RA (CG10011), mRNA
0	0	1	2	3	NM_139922.2	CG13676-RA (CG13676), mRNA
0	0	1	2	2	NM_168667.1	CG32159-RB (CG32159), mRNA
0	0	0	5	5	NM_138159.2	CG7049-RA (CG7049), mRNA
0	0	0	4	7	NM_170281.1	CG5476-RA (CG5476), mRNA
0	0	0	4	4	NM_167324.1	CG32653-RA (CG32653), mRNA
0	0	0	4	3	NM_166516.1	CG11170-RB (CG11170), mRNA
0	0	0	4	2	NM_134922.2	CG3285-RA (CG3285), mRNA
0	0	0	3	6	NM_001038714.1	CG34036-RA (CG34036), mRNA
0	0	0	3	6	NM_166755.3	CG2165-RB, transcript variant B (CG2165), mRNA
0	0	0	3	6	NM_132114.1	CG14442-RA (CG14442), mRNA
0	0	0	3	6	NM_001014688.1	CG2165-RD, transcript variant D (CG2165), mRNA
0	0	0	3	6	NM_166754.3	CG2165-RA, transcript variant A (CG2165), mRNA
0	0	0	3	5	NM_206687.1	CG33235-RA (CG33235), mRNA

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