NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 6335R-2

Symbol Aats-his Full Name Histidyl-tRNA synthetase

CG No CG6335 Old CG No CG6335

Synonyms HRS, CG6335, HisRS, l(1)G0165, l(1)G0358, Aats-his

Accession No (Link to NCBI) NM_167624.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTAAAGACGCCCAAAGGCACAC

Primer Seq. 3'

aatctagaggtaccCTGGAACATGCCGTCCAAAAG

Predicted Fragment Size

IR fragment full Seq

0001 TAAAGACGCC CAAAGGCACA CGGGACTATG GTCCTCAGCA GATGACCCTG CGCCAGGGCG 
0061 TCCTGGACAA GATCGTCCAG GNTGTTCAAG CGCCACGGCg GCGACAGCCA TCGATACGCC 
0121 CGTGTTCGAG CTGAAGGAAG TGCTGACGGG CAAGTACGGC GAGGACTCAA AGCTGATCTA 
0181 CGACTTGAAG GACCAGGGCG GTGAGATCCT ATCGATGAGG TATGACTTGA ACCGTGCCGC 
0241 TGGCTCGTTA CCTGGCGATG AACAAGATCT CGAGCATCAA GCGCTACCAC ATCGCCAAGG 
0301 TCTACCGACG CGACAATCCG GCAATGACCA AGGGTCGCTA TCGGGAGTTC TACCAATGTG 
0361 ACTTCGACAT CGCCGGCACC TACGATCCCA TGCTGCCGGA TGCCGAGTGC GTCAAGATTG 
0421 TGTCGGAGAT ACTGGACACG CTGGACATTG GAGACTATGT GATAAAGCTA AATCACCGCC 
0481 AGCTTTTGGA CGGCATGTTC CAG

in silico PCR Fragment

0001 TAAAGACGCC CAAAGGCACA CGGGACTATG GTCCTCAGCA GATGACCCTG CGCCAGGGCG 
0061 TCCTGGACAA GATCGTCCAG G-TGTTCAAG CGCCACGGCG GCGA-AGCCA TCGATACGCC 
0121 CGTGTTCGAG CTGAAGGAAG TGCTGACGGG CAAGTACGGC GAGGACTCAA AGCTGATCTA 
0181 CGACTTGAAG GATCAGGGCG GTGAGATCCT ATCGATGAGG TATGACTTG- ACCGTGCCGC 
0241 TGGCTCGTTA CCTGGCGATG AACAAGATCT CGAGCATCAA GCGCTACCAC ATCGCCAAGG 
0301 TCTATCGACG CGACAATCCG GCAATGACCA AGGGTCGCTA TCGGGAGTTC TACCAATGTG 
0361 ACTTCGACAT CGCCGGCACC TACGATCCCA TGCTGCCGGA TGCCGAGTGC GTCAAGATTG 
0421 TGTCGGAGAT ACTGGACACG CTGGACATTG GAGACTATGT GATAAAGCTA AATCACCGCC 
0481 AGCTTTTGGA CGGCATGTTC CAG

Assemble Data


6335R-2.IR_full       1   TAAAGACGCCCAAAGGCACACGGGACTATGGTCCTCAGCAGATGACCCTGCGCCAGGGCG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6335R-2.in silico     1   TAAAGACGCCCAAAGGCACACGGGACTATGGTCCTCAGCAGATGACCCTGCGCCAGGGCG 60


6335R-2.IR_full       61  TCCTGGACAAGATCGTCCAGGNTGTTCAAGCGCCACGGCGGCGACAGCCATCGATACGCC 120
                          ||||||||||||||||||||| |||||||||||||||||||||| |||||||||||||||
6335R-2.in silico     61  TCCTGGACAAGATCGTCCAGG-TGTTCAAGCGCCACGGCGGCGA-AGCCATCGATACGCC 120


6335R-2.IR_full       121 CGTGTTCGAGCTGAAGGAAGTGCTGACGGGCAAGTACGGCGAGGACTCAAAGCTGATCTA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6335R-2.in silico     121 CGTGTTCGAGCTGAAGGAAGTGCTGACGGGCAAGTACGGCGAGGACTCAAAGCTGATCTA 180


6335R-2.IR_full       181 CGACTTGAAGGACCAGGGCGGTGAGATCCTATCGATGAGGTATGACTTGAACCGTGCCGC 240
                          |||||||||||| |||||||||||||||||||||||||||||||||||| ||||||||||
6335R-2.in silico     181 CGACTTGAAGGATCAGGGCGGTGAGATCCTATCGATGAGGTATGACTTG-ACCGTGCCGC 240


6335R-2.IR_full       241 TGGCTCGTTACCTGGCGATGAACAAGATCTCGAGCATCAAGCGCTACCACATCGCCAAGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6335R-2.in silico     241 TGGCTCGTTACCTGGCGATGAACAAGATCTCGAGCATCAAGCGCTACCACATCGCCAAGG 300


6335R-2.IR_full       301 TCTACCGACGCGACAATCCGGCAATGACCAAGGGTCGCTATCGGGAGTTCTACCAATGTG 360
                          |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
6335R-2.in silico     301 TCTATCGACGCGACAATCCGGCAATGACCAAGGGTCGCTATCGGGAGTTCTACCAATGTG 360


6335R-2.IR_full       361 ACTTCGACATCGCCGGCACCTACGATCCCATGCTGCCGGATGCCGAGTGCGTCAAGATTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6335R-2.in silico     361 ACTTCGACATCGCCGGCACCTACGATCCCATGCTGCCGGATGCCGAGTGCGTCAAGATTG 420


6335R-2.IR_full       421 TGTCGGAGATACTGGACACGCTGGACATTGGAGACTATGTGATAAAGCTAAATCACCGCC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6335R-2.in silico     421 TGTCGGAGATACTGGACACGCTGGACATTGGAGACTATGTGATAAAGCTAAATCACCGCC 480


6335R-2.IR_full       481 AGCTTTTGGACGGCATGTTCCAG 503
                          |||||||||||||||||||||||
6335R-2.in silico     481 AGCTTTTGGACGGCATGTTCCAG 503

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_167624.1	CG6335-RA, transcript variant A (Aats-his), mRNA
96.47	465	2	3	2	NM_133077.1	CG6335-RB, transcript variant B (Aats-his), mRNA
0	0	6	4	3	NM_139362.2	CG12091-RA (CG12091), mRNA
0	0	4	3	3	NM_139800.2	CG8549-RA (CG8549), mRNA
0	0	3	2	4	NM_138220.1	CG13889-RA (CG13889), mRNA
0	0	2	5	3	NM_001014571.1	CG33556-RA (form3), mRNA
0	0	2	3	5	NM_164818.1	CG13387-RA (emb), mRNA
0	0	2	3	3	NM_143211.2	CG6073-RA (CG6073), mRNA
0	0	2	3	3	NM_137042.2	CG6155-RA (Roe1), mRNA
0	0	2	2	2	NM_137942.1	CG13550-RA (CG13550), mRNA
0	0	1	6	6	NM_132061.1	CG5937-RA (CG5937), mRNA
0	0	1	3	2	NM_140079.1	CG3280-RA (CG3280), mRNA
0	0	1	2	7	NM_143100.1	CG11852-RA (CG11852), mRNA
0	0	1	2	3	NM_165271.1	CG10652-RB, transcript variant B (RpL30), mRNA
0	0	1	2	3	NM_079948.2	CG10652-RA, transcript variant A (RpL30), mRNA
0	0	1	2	3	NM_141261.2	CG1115-RA (CG1115), mRNA
0	0	0	5	2	NM_078635.3	CG12697-RA (Or13a), mRNA
0	0	0	4	18	NM_132335.2	CG12139-RB (CG12139), mRNA
0	0	0	4	6	NM_078854.1	CG18096-RA (TepI), mRNA
0	0	0	4	5	NM_078867.2	CG5526-RA (Dhc36C), mRNA
0	0	0	4	3	NM_141350.1	CG10280-RA (CG10280), mRNA
0	0	0	4	2	NM_078875.2	CG10446-RA (Side), mRNA
0	0	0	3	12	NM_131936.2	CG3556-RA (CG3556), mRNA
0	0	0	3	10	NM_170405.1	CG11956-RB, transcript variant B (SP1029), mRNA
0	0	0	3	10	NM_144361.1	CG11956-RA, transcript variant A (SP1029), mRNA
0	0	0	3	10	NM_170406.1	CG11956-RC, transcript variant C (SP1029), mRNA
0	0	0	3	8	NM_057645.2	CG10844-RC, transcript variant C (Rya-r44F), mRNA
0	0	0	3	8	NM_057646.2	CG10844-RD, transcript variant D (Rya-r44F), mRNA
0	0	0	3	8	NM_057644.2	CG10844-RB, transcript variant B (Rya-r44F), mRNA
0	0	0	3	8	NM_057643.2	CG10844-RA, transcript variant A (Rya-r44F), mRNA

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