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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6331R-2 |
| Symbol |
Orct |
Full Name |
Organic cation transporter |
| CG No |
CG6331 |
Old CG No |
CG6331 |
| Synonyms |
CG6331, Orct |
| Accession No (Link to NCBI) |
NM_079755.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Fan W, Lam SM, Xin J, Yang X, Liu Z, Liu Y, Wang Y, Shui G, Huang X.
Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition.
PLoS Genet (2017) 13(3) e1006664
[
PubMed ID = 28273089
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGTCTGCGCCTTCCACAAGCTG |
| Primer Seq. 3' |
aatctagaggtaccGAAGTGGTGGCGCCCACAATC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NTCTGCGCCT TCCACAAGCT GGCCGGAGTG TTCCTGCTGG CCAAACCCGA CTTCCGATGT
0061 GCCCTGCCAT ACGAAAATGG CAGTATCTAT GAACTATCAC CCCACCTGTG GAACCTATCG
0121 TATCCGGAGA ATGAAAGGTG CTCCTACTAC GATGTGGATT ACACGGAGGA GTACCTGAAT
0181 GGCAGCATTC CACGGAGCAG CAACGAAACC AAGACCCTGT TCCAGCTACG TTTACGATCG
0241 GAGCAAGTAT CTCAATAGTG CGGTGACCGA GTGGAACCTG GTCTGCAGTC GAAGCCTGCT
0301 AAGTGCCACC AGTGATTCGT TATTCATGCT GGGCGTGCTC CTGGGCAGCT TAATCTTTGG
0361 CCAGATGTCC GACAAGCTGG GACGCAAGCC CACCTTCTTC GCCTCACTGG TGCTTCAGCT
0421 AATCTTCGGC GTTTTGGCTG CCGTGGCACC CGAATACTTT AGCTACACGA TTTCCCGTAT
0481 GATTGTGGGC GCCACCACTT C |
| in silico PCR Fragment |
0001 GTCTGCGCCT TCCACAAGCT GGCCGGAGTG TTCCTGCTGG CCAAACCCGA CTTCCGATGT
0061 GCCCTGCCAT ACGAAAATGG CAGTATCTAT GAACTATCAC CCCACCTGTG GAACCTATCG
0121 TATCCGGAGA ATGAAAGGTG CTCCTACTAC GATGTGGATT ACACGGAGGA GTACCTGAAT
0181 GGCAGCATTC CACGGAGCAG CAACGAAACC AAGA-CCTGT TCCAGCTACG TTTACGATCG
0241 GAGCAAGTAT CTCAATAGTG CGGTGACCGA GTGGAACCTG GTCTGCAGTC GAAGCCTGCT
0301 AAGTGCCACC AGTGATTCGT TATTCATGCT GGGCGTGCTC CTGGGCAGCT TAATCTTTGG
0361 CCAGATGTCC GACAAGCTGG GACGCAAGCC CACCTTCTTC GCCTCACTGG TGCTTCAGCT
0421 AATCTTCGGC GTTTTGGCTG CCGTGGCACC CGAATACTTT AGCTACACGA TTTCCCGTAT
0481 GATTGTGGGC GCCACCACTT C |
| Assemble Data |
6331R-2.IR_full 1 TCTGCGCCTTCCACAAGCTGGCCGGAGTGTTCCTGCTGGCCAAACCCGACTTCCGATGT 59
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6331R-2.in silico 1 TCTGCGCCTTCCACAAGCTGGCCGGAGTGTTCCTGCTGGCCAAACCCGACTTCCGATGT 59
6331R-2.IR_full 61 GCCCTGCCATACGAAAATGGCAGTATCTATGAACTATCACCCCACCTGTGGAACCTATCG 119
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6331R-2.in silico 61 GCCCTGCCATACGAAAATGGCAGTATCTATGAACTATCACCCCACCTGTGGAACCTATCG 119
6331R-2.IR_full 121 TATCCGGAGAATGAAAGGTGCTCCTACTACGATGTGGATTACACGGAGGAGTACCTGAAT 179
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6331R-2.in silico 121 TATCCGGAGAATGAAAGGTGCTCCTACTACGATGTGGATTACACGGAGGAGTACCTGAAT 179
6331R-2.IR_full 181 GGCAGCATTCCACGGAGCAGCAACGAAACCAAGACCCTGTTCCAGCTACGTTTACGATCG 239
|||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
6331R-2.in silico 181 GGCAGCATTCCACGGAGCAGCAACGAAACCAAGA-CCTGTTCCAGCTACGTTTACGATCG 239
6331R-2.IR_full 241 GAGCAAGTATCTCAATAGTGCGGTGACCGAGTGGAACCTGGTCTGCAGTCGAAGCCTGCT 299
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6331R-2.in silico 241 GAGCAAGTATCTCAATAGTGCGGTGACCGAGTGGAACCTGGTCTGCAGTCGAAGCCTGCT 299
6331R-2.IR_full 301 AAGTGCCACCAGTGATTCGTTATTCATGCTGGGCGTGCTCCTGGGCAGCTTAATCTTTGG 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6331R-2.in silico 301 AAGTGCCACCAGTGATTCGTTATTCATGCTGGGCGTGCTCCTGGGCAGCTTAATCTTTGG 359
6331R-2.IR_full 361 CCAGATGTCCGACAAGCTGGGACGCAAGCCCACCTTCTTCGCCTCACTGGTGCTTCAGCT 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6331R-2.in silico 361 CCAGATGTCCGACAAGCTGGGACGCAAGCCCACCTTCTTCGCCTCACTGGTGCTTCAGCT 419
6331R-2.IR_full 421 AATCTTCGGCGTTTTGGCTGCCGTGGCACCCGAATACTTTAGCTACACGATTTCCCGTAT 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6331R-2.in silico 421 AATCTTCGGCGTTTTGGCTGCCGTGGCACCCGAATACTTTAGCTACACGATTTCCCGTAT 479
6331R-2.IR_full 481 GATTGTGGGCGCCACCACTTC 500
|||||||||||||||||||||
6331R-2.in silico 481 GATTGTGGGCGCCACCACTTC 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079755.1 |
CG6331-RA (Orct), mRNA |
| 30.7 |
148 |
62 |
36 |
30 |
NM_142981.2 |
CG13610-RA (Orct2), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_137684.1 |
CG9344-RA (CG9344), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_133029.2 |
CG7772-RA (CG7772), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_136423.1 |
CG11101-RA (pwn), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_133145.2 |
CG8010-RA (CG8010), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_135327.2 |
CG7221-RA (Wwox), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_165325.1 |
CG10076-RC, transcript variant C (spir), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_138181.1 |
CG13882-RA (CG13882), mRNA |
| 0 |
0 |
0 |
7 |
10 |
NM_143634.2 |
CG2191-RA (CG2191), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_168364.1 |
CG16707-RB, transcript variant B (vsg), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_140092.1 |
CG16707-RC, transcript variant C (vsg), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_168363.1 |
CG16707-RA, transcript variant A (vsg), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_168362.1 |
CG16707-RD, transcript variant D (vsg), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_169018.1 |
CG31530-RA (CG31530), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_136250.2 |
CG9247-RA (CG9247), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_206126.1 |
CG8400-RB, transcript variant B (CG8400), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_142055.1 |
CG9322-RA (CG9322), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_001043256.1 |
CG34157-RB, transcript variant B (Dys), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_140680.1 |
CG13022-RA (CG13022), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_137236.3 |
CG8400-RA, transcript variant A (CG8400), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_078541.3 |
CG9022-RA (Ost48), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_079504.3 |
CG1133-RA (opa), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_134758.2 |
CG7420-RA (CG7420), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_165168.1 |
CG31738-RA, transcript variant A (CG31738), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_135957.2 |
CG31738-RB, transcript variant B (CG31738), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_132066.1 |
CG4320-RA (raptor), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_134850.2 |
CG3210-RA (Drp1), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_169113.1 |
CG1213-RB, transcript variant B (CG1213), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_141342.1 |
CG1213-RA, transcript variant A (CG1213), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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|
JNK signal
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Caspase-3 signal
| N |
 |
| + |
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| DB |
 |
| ++ |
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| DC |
 |
| ++ |
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| DM |
 |
| + |
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| VM |
 |
| + |
|
| VB |
 |
| |
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