|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6302R-2 |
| Symbol |
l(3)01239 |
Full Name |
lethal (3) 01239 |
| CG No |
CG6302 |
Old CG No |
CG6302 |
| Synonyms |
CG6302, P01239, l(3)j9B4, l(3)01239 |
| Accession No (Link to NCBI) |
NM_168438.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATTGTGGCCCAGTTCCAACAG |
| Primer Seq. 3' |
aatctagaggtaccAACACCAGGACATTACGGTTC |
| Predicted Fragment Size |
383 |
| IR fragment full Seq |
0001 ATTGTGGCCC AGTTCCAACA GCTGCGCAAT GAGCAGCGCA ATTTGGTCAA CAGTTTAAAT
0061 ACGCTCGAAA TGGATTTGAG GGAACACAAA ACTGTGATAG AAACTCTGGA GGCAGGCGGA
0121 TCCCGAACGG AAATGCTTTC GTCAAATTGG TGGTGTGCTG TGCGAAAGGA CCGTGAAGGA
0181 GGTGCTGCCA CAGCTGGTGG AGAACAAGGA TTTCATCGCC AAGACCATCC AAATGGTCAC
0241 CAATGACCTG AGCAAAAAGG GCAGTGAGCT GAACAAGTTC AAGGAGGAGC ACAATATCAA
0301 GATTCGCGGC GAACACTTGG TTGCCGAAGG AGCCAAGGGC GACGATGCGG AGGACAAGGC
0361 GGAGAACCGT AATGTCCTGG TGTT |
| in silico PCR Fragment |
0001 ATTGTGGCCC AGTTCCAACA GCTGCGCAAT GAGCAGCGCA ATTTGGTCAA CAGTTTAAAT
0061 ACGCTCGAAA TGGATTTGAG GGAACACAAA ACTGTGATAG AAACTCTGGA GGC-GGCGGA
0121 TCCCGAACGG AAATGCTTTC GTCAAATTGG TGGTGTGCTG TGCGAAAGGA CCGTGAAGGA
0181 GGTGCTGCCA CAGCTGGTGG AGAACAAGGA TTTCATCGCC AAGACCATCC AAATGGTCAC
0241 CAATGACCTG AGCAAAAAGG GCAGTGAGCT GAACAAGTTC AAGGAGGAGC ACAATATCAA
0301 GATTCGCGGC GAACACTTGG TTGCCGAAGG AGCCAAGGGC GACGATGCGG AGGACAAGGC
0361 GGAGAACCGT AATGTCCTGG TGTT |
| Assemble Data |
6302R-2.IR_full 1 ATTGTGGCCCAGTTCCAACAGCTGCGCAATGAGCAGCGCAATTTGGTCAACAGTTTAAAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6302R-2.in silico 1 ATTGTGGCCCAGTTCCAACAGCTGCGCAATGAGCAGCGCAATTTGGTCAACAGTTTAAAT 60
6302R-2.IR_full 61 ACGCTCGAAATGGATTTGAGGGAACACAAAACTGTGATAGAAACTCTGGAGGCAGGCGGA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
6302R-2.in silico 61 ACGCTCGAAATGGATTTGAGGGAACACAAAACTGTGATAGAAACTCTGGAGGC-GGCGGA 120
6302R-2.IR_full 121 TCCCGAACGGAAATGCTTTCGTCAAATTGGTGGTGTGCTGTGCGAAAGGACCGTGAAGGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6302R-2.in silico 121 TCCCGAACGGAAATGCTTTCGTCAAATTGGTGGTGTGCTGTGCGAAAGGACCGTGAAGGA 180
6302R-2.IR_full 181 GGTGCTGCCACAGCTGGTGGAGAACAAGGATTTCATCGCCAAGACCATCCAAATGGTCAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6302R-2.in silico 181 GGTGCTGCCACAGCTGGTGGAGAACAAGGATTTCATCGCCAAGACCATCCAAATGGTCAC 240
6302R-2.IR_full 241 CAATGACCTGAGCAAAAAGGGCAGTGAGCTGAACAAGTTCAAGGAGGAGCACAATATCAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6302R-2.in silico 241 CAATGACCTGAGCAAAAAGGGCAGTGAGCTGAACAAGTTCAAGGAGGAGCACAATATCAA 300
6302R-2.IR_full 301 GATTCGCGGCGAACACTTGGTTGCCGAAGGAGCCAAGGGCGACGATGCGGAGGACAAGGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6302R-2.in silico 301 GATTCGCGGCGAACACTTGGTTGCCGAAGGAGCCAAGGGCGACGATGCGGAGGACAAGGC 360
6302R-2.IR_full 361 GGAGAACCGTAATGTCCTGGTGTT 384
||||||||||||||||||||||||
6302R-2.in silico 361 GGAGAACCGTAATGTCCTGGTGTT 384
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
365 |
0 |
0 |
0 |
NM_168438.1 |
CG6302-RA (l(3)01239), mRNA |
| 0 |
0 |
4 |
3 |
14 |
NM_137151.2 |
CG10212-RA (SMC2), mRNA |
| 0 |
0 |
4 |
3 |
5 |
NM_142385.1 |
CG14322-RA (CG14322), mRNA |
| 0 |
0 |
3 |
6 |
2 |
NM_143713.2 |
CG2173-RA (Rs1), mRNA |
| 0 |
0 |
3 |
5 |
4 |
NM_143285.1 |
CG6051-RA (CG6051), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_134975.2 |
CG3702-RA (CG3702), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_143084.1 |
CG11847-RA (CG11847), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_001014453.1 |
CG33526-RD, transcript variant D (PNUTS), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_001014452.1 |
CG33526-RC, transcript variant C (PNUTS), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_001014455.1 |
CG33526-RB, transcript variant B (PNUTS), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_001014454.1 |
CG33526-RA, transcript variant A (PNUTS), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_167796.2 |
CG7051-RA, transcript variant A (CG7051), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_138157.2 |
CG7051-RB, transcript variant B (CG7051), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_137721.1 |
CG18375-RA, transcript variant A (CG18375), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_176243.1 |
CG18375-RB, transcript variant B (CG18375), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_142292.1 |
CG14897-RB, transcript variant B (CG14897), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_135112.1 |
CG11030-RA (CG11030), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_176144.1 |
CG33144-RA (CG33144), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_132588.1 |
CG15728-RA (CG15728), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_078705.2 |
CG1685-RA (pen), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_143150.3 |
CG4673-RA, transcript variant A (CG4673), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_170231.2 |
CG4673-RB, transcript variant B (CG4673), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_136826.1 |
CG7741-RA (CG7741), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_137492.1 |
CG17669-RA (CG17669), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_057388.2 |
CG9224-RA (sog), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_057356.3 |
CG2595-RA, transcript variant A (RacGAP84C), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_057355.3 |
CG2595-RB, transcript variant B (RacGAP84C), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_142974.2 |
CG17786-RA (CG17786), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_136813.1 |
CG9077-RA (CG9077), mRNA |
| 0 |
0 |
0 |
8 |
5 |
NM_001032002.1 |
CG33676-RA, transcript variant A (Skeletor), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| + |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
|
|
