NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

6284R-4

Symbol

Sirt6

Full Name

Sirt6

CG No

CG6284

Old CG No

CG6284

Synonyms

dmSRT408, CG6284, dSIRT6, D.mel4, Sirt6

Accession No (Link to NCBI)

NM_141733.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

abnormal wing

Map Viewer

Reference
[Please submit your publication]

Kusama S, Ueda R, Suda T, Nishihara S, Matsuura ET.
Involvement of Drosophila Sir2-like genes in the regulation of life span.
Genes Genet Syst (2006) 81(5) 341-8 [ PubMed ID = 17159295 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgACTACGCGGATGGATTGTCAG

Primer Seq. 3'

aatctagaggtaccGATCTACAGCTACGACCTTTG

Predicted Fragment Size

IR fragment full Seq

0001 ACTACGCGGA TGGATTGTCA GCCTACGACA ACAAGGGAAT TTTGGGAGCA CCAGAGAGTT 
0061 TCGACAGCGA TGAGGTTGTG GCCGAAAAGT GCCAGGAATT GGCTGAATTG ATCAAGAAAT 
0121 CGGGACACGT TGTCCTCCAC ACGGGAGCTG GGATCAGTAC GTCTGCAGGA ATTCCGGATT 
0181 TCCGCGGACC CAAGGGCGTT TGGACCCTGG AGGAGAAGGG CGAGAAGCCG GACTTCAATG 
0241 TTTCCTTCGA TGAAGCCAGA CCAACTAAAA CCCACATGGC TATCATAGCC CTGATTGAAA 
0301 GTGGCTATGT GCAGTACGTA ATCTCACAGA ATATTGATGG TCTCCACTTG AAATCCGGAC 
0361 TGGATCGGAA GTATCTTTCC GAATTGCACG GCAACATTTA CATCGAACAG TGTAAGAAAT 
0421 GCAGACGGCA ATTTGTGAGC CCATCTGCCG TGGAAACAGT GGGTCAAAAA TCCCTGCAAC 
0481 GTGCCTGCAA GTCTTCAATG GATAGCAAAG GTCGTAGCTG TAGATC

in silico PCR Fragment

0001 ACTACGCGGA TGGATTGTCA GCCTACGACA ACAAGGGAAT TTTGGGAGCA CCAGAGAGTT 
0061 TCGACAGCGA TGAGGTTGTG GCCGAAAAGT GCCAGGAATT GGCTGAATTG ATCAAGAAAT 
0121 CGGGACACGT TGTCCTCCAC ACGGGAGCTG GGATCAGTAC GTCTGCAGGA ATTCCGGATT 
0181 TCCGCGGACC CAAGGGCGTT TGGACCCTGG AGGAGAAGGG CGAGAAGCCG GACTTCAATG 
0241 TTTCCTTCGA TGAAGCCAGA CCAACTAAAA CCCACATGGC TATCATAGCC CTGATTGAAA 
0301 GTGGCTATGT GCAGTACGTA ATCTCACAGA ATATTGATGG TCTCCACTTG AAATCCGGAC 
0361 TGGATCGGAA GTATCTTTCC GAATTGCACG GCAACATTTA CATCGAACAG TGTAAGAAAT 
0421 GCAGACGGCA ATTTGTGAGC CCATCTGCCG TGGAAACAGT GGGTCAAAAA TCCCTGCAAC 
0481 GTGCCTGCAA GTCTTCAATG GATAGCAAAG GTCGTAGCTG TAGATC

Assemble Data


6284R-4.IR_full       1   ACTACGCGGATGGATTGTCAGCCTACGACAACAAGGGAATTTTGGGAGCACCAGAGAGTT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6284R-4.in silico     1   ACTACGCGGATGGATTGTCAGCCTACGACAACAAGGGAATTTTGGGAGCACCAGAGAGTT 60


6284R-4.IR_full       61  TCGACAGCGATGAGGTTGTGGCCGAAAAGTGCCAGGAATTGGCTGAATTGATCAAGAAAT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6284R-4.in silico     61  TCGACAGCGATGAGGTTGTGGCCGAAAAGTGCCAGGAATTGGCTGAATTGATCAAGAAAT 120


6284R-4.IR_full       121 CGGGACACGTTGTCCTCCACACGGGAGCTGGGATCAGTACGTCTGCAGGAATTCCGGATT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6284R-4.in silico     121 CGGGACACGTTGTCCTCCACACGGGAGCTGGGATCAGTACGTCTGCAGGAATTCCGGATT 180


6284R-4.IR_full       181 TCCGCGGACCCAAGGGCGTTTGGACCCTGGAGGAGAAGGGCGAGAAGCCGGACTTCAATG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6284R-4.in silico     181 TCCGCGGACCCAAGGGCGTTTGGACCCTGGAGGAGAAGGGCGAGAAGCCGGACTTCAATG 240


6284R-4.IR_full       241 TTTCCTTCGATGAAGCCAGACCAACTAAAACCCACATGGCTATCATAGCCCTGATTGAAA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6284R-4.in silico     241 TTTCCTTCGATGAAGCCAGACCAACTAAAACCCACATGGCTATCATAGCCCTGATTGAAA 300


6284R-4.IR_full       301 GTGGCTATGTGCAGTACGTAATCTCACAGAATATTGATGGTCTCCACTTGAAATCCGGAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6284R-4.in silico     301 GTGGCTATGTGCAGTACGTAATCTCACAGAATATTGATGGTCTCCACTTGAAATCCGGAC 360


6284R-4.IR_full       361 TGGATCGGAAGTATCTTTCCGAATTGCACGGCAACATTTACATCGAACAGTGTAAGAAAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6284R-4.in silico     361 TGGATCGGAAGTATCTTTCCGAATTGCACGGCAACATTTACATCGAACAGTGTAAGAAAT 420


6284R-4.IR_full       421 GCAGACGGCAATTTGTGAGCCCATCTGCCGTGGAAACAGTGGGTCAAAAATCCCTGCAAC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6284R-4.in silico     421 GCAGACGGCAATTTGTGAGCCCATCTGCCGTGGAAACAGTGGGTCAAAAATCCCTGCAAC 480


6284R-4.IR_full       481 GTGCCTGCAAGTCTTCAATGGATAGCAAAGGTCGTAGCTGTAGATC 526
                          ||||||||||||||||||||||||||||||||||||||||||||||
6284R-4.in silico     481 GTGCCTGCAAGTCTTCAATGGATAGCAAAGGTCGTAGCTGTAGATC 526

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	508	0	0	1	NM_141733.1	CG6284-RA (Sirt6), mRNA
0.19	1	3	2	2	NM_135491.2	CG31755-RA (CG31755), mRNA
0	0	3	3	7	NM_140963.1	CG5847-RA (CG5847), mRNA
0	0	2	3	3	NM_080325.3	CG6450-RC (lva), mRNA
0	0	2	2	3	NM_001043040.1	CG17800-PAJ (Dscam), mRNA
0	0	2	2	3	NM_001043041.1	CG17800-PBE (Dscam), mRNA
0	0	2	2	3	NM_001043045.1	CG17800-PAK (Dscam), mRNA
0	0	2	2	3	NM_001043014.1	CG17800-PAL (Dscam), mRNA
0	0	2	2	3	NM_001043057.1	CG17800-PAM (Dscam), mRNA
0	0	2	2	3	NM_001043038.1	CG17800-PAN (Dscam), mRNA
0	0	2	2	3	NM_001043059.1	CG17800-PAO (Dscam), mRNA
0	0	2	2	3	NM_001043051.1	CG17800-PAP (Dscam), mRNA
0	0	2	2	3	NM_001043023.1	CG17800-PAQ (Dscam), mRNA
0	0	2	2	3	NM_001043027.1	CG17800-PAR (Dscam), mRNA
0	0	2	2	3	NM_001043019.1	CG17800-PAS (Dscam), mRNA
0	0	2	2	3	NM_078925.5	CG17800-RD, transcript variant D (Dscam), mRNA
0	0	2	2	3	NM_001043021.1	CG17800-PAT (Dscam), mRNA
0	0	2	2	3	NM_001043037.1	CG17800-PAU (Dscam), mRNA
0	0	2	2	3	NM_001043039.1	CG17800-PAV (Dscam), mRNA
0	0	2	2	3	NM_001043017.1	CG17800-PAW (Dscam), mRNA
0	0	2	2	3	NM_001043015.1	CG17800-PAX (Dscam), mRNA
0	0	2	2	3	NM_001043032.1	CG17800-PAY (Dscam), mRNA
0	0	2	2	3	NM_001043024.1	CG17800-PBC (Dscam), mRNA
0	0	2	2	3	NM_001043035.1	CG17800-RT, transcript variant T (Dscam), mRNA
0	0	2	2	3	NM_001043046.1	CG17800-RU, transcript variant U (Dscam), mRNA
0	0	2	2	3	NM_001043018.1	CG17800-RV, transcript variant V (Dscam), mRNA
0	0	2	2	3	NM_206047.1	CG17800-RE, transcript variant E (Dscam), mRNA
0	0	2	2	3	NM_001043056.1	CG17800-RW, transcript variant W (Dscam), mRNA
0	0	2	2	3	NM_001043030.1	CG17800-RX, transcript variant X (Dscam), mRNA
0	0	2	2	3	NM_001043026.1	CG17800-RY, transcript variant Y (Dscam), mRNA

Back