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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 6282R-2 
 Symbol CG6282  Full Name CG6282 
 CG No CG6282  Old CG No CG6282 
 Synonyms CG6282 
 Accession No (Link to NCBI) NM_139986.2 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGGAGCCTGCAGTTGGTCTTC 
 Primer Seq. 3' 
aatctagaggtaccGACACGCTCCACAGTCCATCA 
 Predicted Fragment Size 
524 
 IR fragment full Seq 
0001 GGGAGCCTGC AGTTGGTCTT CTTTCTGATC AACTCTGTGC TCCAAATGGA CAAGCTGACG 
0061 GACTTTGCCG GCGGCGTTAA CTTCATCATC ATCTCCCTGC TCACTTTCTT TCTCGGCCAA 
0121 ATCGATCGTC CAGTCGAAGG CCTACGATAG TCGCCAGCTG ATGGTCACGC TCTTCGTTTG 
0181 CCTGTGGGGC GCTCGGCTGT CCGGATATTT ACTATATCGA ATCGTAAAGT TGGGTCGCGA 
0241 CAAACAGTTC GAGGATACCC GTCGCAACAT CATCCGCTAT GCGGTCTTCT GGACCTTCCA 
0301 GGCTGTTTGG GTGTACATTG TGTCACTGCC TGTAATAATA ATTAATTCAC CGCGCCACTC 
0361 GCAGCCGCGT GCCCCAAAGA CCATGACCAC ACTGGACTCC ACGGGCACGG GCATGTTCAT 
0421 CGTTGGCCTG CTGGCGGAGA CCTATGCGGA TCTGCAGAAG TTCTCCTTCC GCCAGGATCC 
0481 GGCCAATCAG GGCAAGTTCT GCAATGATGG ACTGTGGAGC GTGTC 
 in silico PCR Fragment 
0001 GGGAGCCTGC AGTTGGTCTT CTTTCTGATC AACTCTGTGC TCCAAATGGA CAAGCTGACG 
0061 GACTTTGCCG GCGGCGTTAA CTTCATCATC ATCTCCCTGC TCACTTTCTT TCTCGGCCAA 
0121 ATCGATCGTC CA-TCGAAGG CCTACGATAG TCGCCAGCTG ATGGTCACGC TCTTCGTTTG 
0181 CCTGTGGGGC GCTCGGCTGT CCGGATATTT ACTATATCGA ATCGTAAAGT TGGGTCGCGA 
0241 CAAACAGTTC GAGGATACCC GTCGCAACAT CATCCGCTAT GCGGTCTTCT GGACCTTCCA 
0301 GGCTGTTTGG GTGTACATTG TGTCACTGCC TGTAATAATA ATTAATTCAC CGCGCCACTC 
0361 GCAGCCGCGT GCCCCAAAGA CCATGACCAC ACTGGACTCC ACGGGCACGG GCATGTTCAT 
0421 CGTTGGCCTG CTGGCGGAGA CCTATGCGGA TCTGCAGAAG TTCTCCTTCC GCCAGGATCC 
0481 GGCCAATCAG GGCAAGTTCT GCAATGATGG ACTGTGGAGC GTGTC 
 Assemble Data 

6282R-2.IR_full       1   GGGAGCCTGCAGTTGGTCTTCTTTCTGATCAACTCTGTGCTCCAAATGGACAAGCTGACG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6282R-2.in silico     1   GGGAGCCTGCAGTTGGTCTTCTTTCTGATCAACTCTGTGCTCCAAATGGACAAGCTGACG 60


6282R-2.IR_full       61  GACTTTGCCGGCGGCGTTAACTTCATCATCATCTCCCTGCTCACTTTCTTTCTCGGCCAA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6282R-2.in silico     61  GACTTTGCCGGCGGCGTTAACTTCATCATCATCTCCCTGCTCACTTTCTTTCTCGGCCAA 120


6282R-2.IR_full       121 ATCGATCGTCCAGTCGAAGGCCTACGATAGTCGCCAGCTGATGGTCACGCTCTTCGTTTG 180
                          |||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
6282R-2.in silico     121 ATCGATCGTCCA-TCGAAGGCCTACGATAGTCGCCAGCTGATGGTCACGCTCTTCGTTTG 180


6282R-2.IR_full       181 CCTGTGGGGCGCTCGGCTGTCCGGATATTTACTATATCGAATCGTAAAGTTGGGTCGCGA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6282R-2.in silico     181 CCTGTGGGGCGCTCGGCTGTCCGGATATTTACTATATCGAATCGTAAAGTTGGGTCGCGA 240


6282R-2.IR_full       241 CAAACAGTTCGAGGATACCCGTCGCAACATCATCCGCTATGCGGTCTTCTGGACCTTCCA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6282R-2.in silico     241 CAAACAGTTCGAGGATACCCGTCGCAACATCATCCGCTATGCGGTCTTCTGGACCTTCCA 300


6282R-2.IR_full       301 GGCTGTTTGGGTGTACATTGTGTCACTGCCTGTAATAATAATTAATTCACCGCGCCACTC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6282R-2.in silico     301 GGCTGTTTGGGTGTACATTGTGTCACTGCCTGTAATAATAATTAATTCACCGCGCCACTC 360


6282R-2.IR_full       361 GCAGCCGCGTGCCCCAAAGACCATGACCACACTGGACTCCACGGGCACGGGCATGTTCAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6282R-2.in silico     361 GCAGCCGCGTGCCCCAAAGACCATGACCACACTGGACTCCACGGGCACGGGCATGTTCAT 420


6282R-2.IR_full       421 CGTTGGCCTGCTGGCGGAGACCTATGCGGATCTGCAGAAGTTCTCCTTCCGCCAGGATCC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6282R-2.in silico     421 CGTTGGCCTGCTGGCGGAGACCTATGCGGATCTGCAGAAGTTCTCCTTCCGCCAGGATCC 480


6282R-2.IR_full       481 GGCCAATCAGGGCAAGTTCTGCAATGATGGACTGTGGAGCGTGTC 525
                          |||||||||||||||||||||||||||||||||||||||||||||
6282R-2.in silico     481 GGCCAATCAGGGCAAGTTCTGCAATGATGGACTGTGGAGCGTGTC 525



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   506  0  0  0  NM_139986.2  CG6282-RA, transcript variant A (CG6282), mRNA 
100   506  0  0  0  NM_176302.1  CG6282-RB, transcript variant B (CG6282), mRNA 
0   0  7  6  5  NM_136721.2  CG12209-RA (CG12209), mRNA 
0   0  5  3  3  NM_167689.1  CG32821-RA (CG32821), mRNA 
0   0  4  3  3  NM_137809.1  CG3292-RA (CG3292), mRNA 
0   0  3  4  3  NM_170405.1  CG11956-RB, transcript variant B (SP1029), mRNA 
0   0  3  4  3  NM_144361.1  CG11956-RA, transcript variant A (SP1029), mRNA 
0   0  3  4  3  NM_170406.1  CG11956-RC, transcript variant C (SP1029), mRNA 
0   0  2  4  6  NM_135076.2  CG14024-RA (CG14024), mRNA 
0   0  2  3  7  NM_140067.1  CG3654-RD (CG3654), mRNA 
0   0  2  3  5  NM_206502.1  CG3937-RD, transcript variant D (cher), mRNA 
0   0  2  3  5  NM_079659.2  CG3937-RA, transcript variant A (cher), mRNA 
0   0  2  3  2  NM_132771.1  CG15027-RA (CG15027), mRNA 
0   0  2  2  2  NM_168571.2  CG32133-RA (CG32133), mRNA 
0   0  1  5  5  NM_135077.2  CG14023-RA (CG14023), mRNA 
0   0  1  3  6  NM_134601.1  CG1718-RA (CG1718), mRNA 
0   0  1  3  6  NM_132370.1  CG2989-RA (CG2989), mRNA 
0   0  1  3  5  NM_175976.1  CG14026-RC, transcript variant C (tkv), mRNA 
0   0  1  3  5  NM_175977.1  CG14026-RB, transcript variant B (tkv), mRNA 
0   0  1  3  5  NM_175978.1  CG14026-RD, transcript variant D (tkv), mRNA 
0   0  1  3  5  NM_175975.1  CG14026-RA, transcript variant A (tkv), mRNA 
0   0  1  3  3  NM_170548.1  CG1607-RB, transcript variant B (CG1607), mRNA 
0   0  1  3  3  NM_143608.1  CG1607-RA, transcript variant A (CG1607), mRNA 
0   0  1  3  2  NM_140763.1  CG7408-RB (CG7408), mRNA 
0   0  1  2  5  NM_170079.2  CG31145-RA, transcript variant A (CG31145), mRNA 
0   0  1  2  5  NM_176547.1  CG31145-RB, transcript variant B (CG31145), mRNA 
0   0  1  2  3  NM_135281.1  CG6630-RA (CG6630), mRNA 
0   0  1  2  3  NM_136005.1  CG15144-RA (CG15144), mRNA 
0   0  1  2  2  NM_165882.1  CG8493-RB, transcript variant B (CG8493), mRNA 
0   0  1  2  2  NM_167774.1  CG17686-RA, transcript variant A (DIP1), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
+ 
DC
+ 
DM
+ 
VM
 
VB
 
Dcompartment   Penetrance
     

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