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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6113R-3 |
| Symbol |
CG6113 |
Full Name |
CG6113 |
| CG No |
CG6113 |
Old CG No |
CG6113 |
| Synonyms |
CG6113 |
| Accession No (Link to NCBI) |
NM_135574.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAAGATCCGTGGCGGTGGATA |
| Primer Seq. 3' |
aatctagaggtaccGGCCATGATCCAGAACACAAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NAAGATCCGT GGCGNGTGGA TAGCAATGTG CGGTTGGAGA ACGATGTGGA TCCCAATATT
0061 CAGGAGGATT NCACACTTGA ATACCTACAG TCTGATAAAA AAGTACGGCT ACCCGGCGGA
0121 GAATCACACC TTGGAGACGG ATGATGGATA CATCCTCACC TTGCACAGGA TCGCTAGACC
0181 GGGAGCAACG CCAGTGCNTT CTGGTGCACG GACTACTGGA TAGCTCAGCC ACTTGGGTGA
0241 TGATGGGACC GAACAAGGGA CTTGGTTACC TGCTCTACGA TCAGGGGTAC GATGTCTGGA
0301 TGGCCAATGT TCGTGGCAAT ACGTACTCGA GAAAGCATGT TAAGTATAGC ACCCACCATG
0361 CCAAGTTCTG GGACTTTACA TTCCACGAGA TGGGAAAGCA TGATATACCC GCCACAATGG
0421 ACTACATCCT CAATTCCACG GGCGTAAGCC AGATTGCACT ACATTGGTCA CTCCCAGGGA
0481 ACCGTTGTGT TCTGGATcaT GGNC |
| in silico PCR Fragment |
0001 GAAGATCCGT GGCG-GTGGA TAGCAATGTG CGGTTGGAGA ACGATGTGGA TCCCAATATT
0061 CAGGAGGATT -CACACTTGA ATACCTACAG TCTGATAAAA AAGTACGGCT ACCCGGCGGA
0121 GAATCACACC TTGGAGACGG ATGATGGATA CATCCTCACC TTGCACAGGA TCGCTAGACC
0181 GGGAGCAACG CCAGTGC-TT CTGGTGCACG GACTACTGGA TAGCTCAGCC ACTTGGGTGA
0241 TGATGGGACC GAACAAGGGA CTTGGTTACC TGCTCTACGA TCAGGGGTAC GATGTCTGGA
0301 TGGCCAATGT TCGTGGCAAT ACGTACTCGA GAAAGCATGT TAAGTATAGC ACCCACCATG
0361 CCAAGTTCTG GGACTTTACA TTCCACGAGA TGGGAAAGCA TGATATACCC GCCACAATGG
0421 ACTACATCCT CAATTCCACG GGCGTAAGCC A-ATTGCACT ACATTGGTCA CTCCCAGGGA
0481 ACCGTTGTGT TCTGGATCAT GGCC |
| Assemble Data |
6113R-3.IR_full 1 AAGATCCGTGGCGNGTGGATAGCAATGTGCGGTTGGAGAACGATGTGGATCCCAATATT 59
||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
6113R-3.in silico 1 AAGATCCGTGGCG-GTGGATAGCAATGTGCGGTTGGAGAACGATGTGGATCCCAATATT 59
6113R-3.IR_full 61 CAGGAGGATTNCACACTTGAATACCTACAGTCTGATAAAAAAGTACGGCTACCCGGCGGA 119
|||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
6113R-3.in silico 61 CAGGAGGATT-CACACTTGAATACCTACAGTCTGATAAAAAAGTACGGCTACCCGGCGGA 119
6113R-3.IR_full 121 GAATCACACCTTGGAGACGGATGATGGATACATCCTCACCTTGCACAGGATCGCTAGACC 179
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6113R-3.in silico 121 GAATCACACCTTGGAGACGGATGATGGATACATCCTCACCTTGCACAGGATCGCTAGACC 179
6113R-3.IR_full 181 GGGAGCAACGCCAGTGCNTTCTGGTGCACGGACTACTGGATAGCTCAGCCACTTGGGTGA 239
||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
6113R-3.in silico 181 GGGAGCAACGCCAGTGC-TTCTGGTGCACGGACTACTGGATAGCTCAGCCACTTGGGTGA 239
6113R-3.IR_full 241 TGATGGGACCGAACAAGGGACTTGGTTACCTGCTCTACGATCAGGGGTACGATGTCTGGA 299
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6113R-3.in silico 241 TGATGGGACCGAACAAGGGACTTGGTTACCTGCTCTACGATCAGGGGTACGATGTCTGGA 299
6113R-3.IR_full 301 TGGCCAATGTTCGTGGCAATACGTACTCGAGAAAGCATGTTAAGTATAGCACCCACCATG 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6113R-3.in silico 301 TGGCCAATGTTCGTGGCAATACGTACTCGAGAAAGCATGTTAAGTATAGCACCCACCATG 359
6113R-3.IR_full 361 CCAAGTTCTGGGACTTTACATTCCACGAGATGGGAAAGCATGATATACCCGCCACAATGG 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6113R-3.in silico 361 CCAAGTTCTGGGACTTTACATTCCACGAGATGGGAAAGCATGATATACCCGCCACAATGG 419
6113R-3.IR_full 421 ACTACATCCTCAATTCCACGGGCGTAAGCCAGATTGCACTACATTGGTCACTCCCAGGGA 479
||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
6113R-3.in silico 421 ACTACATCCTCAATTCCACGGGCGTAAGCCA-ATTGCACTACATTGGTCACTCCCAGGGA 479
6113R-3.IR_full 481 ACCGTTGTGTTCTGGATCATGGNC 503
|||||||||||||||||||||| |
6113R-3.in silico 481 ACCGTTGTGTTCTGGATCATGGCC 503
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_135574.2 |
CG6113-RA (CG6113), mRNA |
| 1.86 |
9 |
14 |
29 |
48 |
NM_135575.1 |
CG18301-RA (CG18301), mRNA |
| 0 |
0 |
9 |
22 |
24 |
NM_164939.1 |
CG31871-RA (CG31871), mRNA |
| 0 |
0 |
3 |
9 |
19 |
NM_135584.2 |
CG18284-RA (CG18284), mRNA |
| 0 |
0 |
3 |
7 |
21 |
NM_135581.1 |
CG7329-RA (CG7329), mRNA |
| 0 |
0 |
3 |
7 |
15 |
NM_164937.1 |
CG31872-RA (CG31872), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_136687.2 |
CG1472-RA (sec24), mRNA |
| 0 |
0 |
2 |
1 |
1 |
NM_135029.2 |
CG3036-RA (CG3036), mRNA |
| 0 |
0 |
1 |
6 |
9 |
NM_135887.2 |
CG3491-RA (CG3491), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_134617.2 |
CG14614-RA (CG14614), mRNA |
| 0 |
0 |
1 |
2 |
9 |
NM_079175.2 |
CG1242-RA (Hsp83), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_136683.2 |
CG1516-RE, transcript variant E (CG1516), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165709.1 |
CG1516-RI, transcript variant I (CG1516), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165705.1 |
CG1516-RA, transcript variant A (CG1516), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165710.1 |
CG1516-RJ, transcript variant J (CG1516), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165707.1 |
CG1516-RD, transcript variant D (CG1516), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165706.1 |
CG1516-RB, transcript variant B (CG1516), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165711.1 |
CG1516-RK, transcript variant K (CG1516), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165708.1 |
CG1516-RG, transcript variant G (CG1516), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165712.1 |
CG1516-RL, transcript variant L (CG1516), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_142231.1 |
CG9590-RA (CG9590), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169054.1 |
CG12162-RB, transcript variant B (CG12162), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_134932.2 |
CG2772-RA (CG2772), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_167480.1 |
CG9209-RC, transcript variant C (vap), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_206759.1 |
CG9209-RD, transcript variant D (vap), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_078637.2 |
CG9209-RB, transcript variant B (vap), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_167481.2 |
CG9209-RA, transcript variant A (vap), mRNA |
| 0 |
0 |
0 |
5 |
23 |
NM_078816.2 |
CG7279-RA (Lip1), mRNA |
| 0 |
0 |
0 |
5 |
15 |
NM_136294.3 |
CG3635-RA (CG3635), mRNA |
| 0 |
0 |
0 |
5 |
9 |
NM_057983.2 |
CG8823-RA (Lip3), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
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