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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5993R-2 |
| Symbol |
os |
Full Name |
outstretched |
| CG No |
CG5993 |
Old CG No |
CG5993 |
| Synonyms |
Upd, upd, CG5993, sisC, sis-c, unp, UPD, sis-C, od, upa, sy, odsy, l(1)YM55, l(1)YC43, bdw, os, outstretched, bending-wings, outstretched-smalleye, sisterless c, small-eye, unpaired, Unpaired, Os, OS, upd1 |
| Accession No (Link to NCBI) |
NM_080356.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Nagata R, Akai N, Kondo S, Saito K, Ohsawa S, Igaki T.
Yorkie drives supercompetition by non-autonomous induction of autophagy via bantam microRNA in Drosophila.
Curr Biol (2022) 32(5) 1064-1076.e4
[
PubMed ID = 35134324
]
[
RRC reference
]
Cao X, Rojas M, Pastor-Pareja JC.
Intrinsic and damage-induced JAK/STAT signaling regulate developmental timing by the Drosophila prothoracic gland.
Dis Model Mech (2022) 15(1)
[
PubMed ID = 34842272
]
[
RRC reference
]
Juarez-Carreño S, Vallejo DM, Carranza-Valencia J, Palomino-Schätzlein M, Ramon-Cañellas P, Santoro R, de Hartog E, Ferres-Marco D, Romero A, Peterson HP, Ballesta-Illan E, Pineda-Lucena A, Dominguez M, Morante J.
Body-fat sensor triggers ribosome maturation in the steroidogenic gland to initiate sexual maturation in Drosophila.
Cell Rep (2021) 37(2) 109830
[
PubMed ID = 34644570
]
[
RRC reference
]
Park J, Lee J, Kim JH, Lee J, Park H, Lim C.
ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD.
Nucleic Acids Res (2021) 49(19) 11294-11311
[
PubMed ID = 34551427
]
[
RRC reference
]
Enomoto M, Takemoto D, Igaki T.
Interaction between Ras and Src clones causes interdependent tumor malignancy via Notch signaling in Drosophila.
Dev Cell (2021) 56(15) 2223-2236.e5
[
PubMed ID = 34324859
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Álvarez-Fernández C, Tamirisa S, Prada F, Chernomoretz A, Podhajcer O, Blanco E, Martín-Blanco E.
Identification and functional analysis of healing regulators in Drosophila.
PLoS Genet (2015) 11(2) e1004965
[
PubMed ID = 25647511
]
[
RRC reference
]
Ohsawa S, Sato Y, Enomoto M, Nakamura M, Betsumiya A, Igaki T.
Mitochondrial defect drives non-autonomous tumour progression through Hippo signalling in Drosophila.
Nature (2012) 490(7421) 547-51
[
PubMed ID = 23023132
]
[
RRC reference
]
Enomoto M, Igaki T.
Src controls tumorigenesis via JNK-dependent regulation of the Hippo pathway in Drosophila.
EMBO Rep (2013) 14(1) 65-72
[
PubMed ID = 23196366
]
[
RRC reference
]
Borensztejn A, Boissoneau E, Fernandez G, Agnès F, Pret AM.
JAK/STAT autocontrol of ligand-producing cell number through apoptosis.
Development (2013) 140(1) 195-204
[
PubMed ID = 23222440
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCAACATGCAAACTTACGTGG |
| Primer Seq. 3' |
aatctagaggtaccCTTTTTCTGGCTCCTCTGCTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCAAGCATGC AAACTTACGT GGCCAGCTTT GCGTATCTGA GGCGCCAGCA GATCCGCTGG
0061 GATCAGCGGT CCATCACCCG CGAGTCCAGC ACCGCCCGCG AGCTgcGCGA GCTGCTCCTG
0121 AGCTCGCGGC GCATCCTCTG CGAGCTGGAG ACAGCCGTCA ACCAGACGCA GAGTCCGCGC
0181 CAGAAGCAGC GGCGCAGTGG TGCGGCGGTC ACAGCAGTTG GCGGCACCAC CATGCTGGGT
0241 CAGCAGCTGC CACAGATCTC GCGTCTCGAG ATGAACAAGC GTTTGAAGCT GCGCTCCAAG
0301 ACGAGTGGGC CCGGTATGGG TGGTGCTGCT TCCAGTGCAT CCATGGCGGC GGGCGAGGCG
0361 GACTCGATCG ATATGCGCTT TGTGAAGCAC CACTACTACG ACTTTTTGCG CACCATGTAC
0421 CAGCTGCTGC GGCGGGATGG CAAGCGGGTA AGGAGTCGGC CACGGAAGCA CCACAAGAAG
0481 CAGCAGAGGA GCCAGAAAAA G |
| in silico PCR Fragment |
0001 GCAA-CATGC AAACTTACGT GGCCAGCTTT GCGTATCTGA GGCGCCAGCA GATCCGCTGG
0061 GATCAGCGGT CCATCACCCG CGAGTCCAGC ACCGCCCGCG AGCTGCGCGA GCTGCTCCTG
0121 AGCTCGCGGC GCATCCTCTG CGAGCTGGAG ACAGCCGTCA ACCAGACGCA GAGTCCGCGC
0181 CAGAAGCAGC GGCGCAGTGG TGCGGCGGTC ACAGCAGTTG GCGGCACCAC CATGCTGGGT
0241 CAGCAGCTGC CACAGATCTC GCGTCTCGAG ATGAACAAGC GTTTGAAGCT GCGCTCCAAG
0301 ACGAGTGGGC CCGGTATGGG TGGTGCTGCT TCCAGTGCAT CCATGGCGGC GGGCGAGGCG
0361 GACTCGATCG ATATGCGCTT TGTGAAGCAC CACTACTACG ACTTTTTGCG CACCATGTAC
0421 CAGCTGCTGC GGCGGGATGG CAAGCGGGTA AGGAGTCGGC CACGGAAGCA CCACAAGAAG
0481 CAGCAGAGGA GCCAGAAAAA G |
| Assemble Data |
5993R-2.IR_full 1 GCAAGCATGCAAACTTACGTGGCCAGCTTTGCGTATCTGAGGCGCCAGCAGATCCGCTGG 60
|||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
5993R-2.in silico 1 GCAA-CATGCAAACTTACGTGGCCAGCTTTGCGTATCTGAGGCGCCAGCAGATCCGCTGG 60
5993R-2.IR_full 61 GATCAGCGGTCCATCACCCGCGAGTCCAGCACCGCCCGCGAGCTGCGCGAGCTGCTCCTG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5993R-2.in silico 61 GATCAGCGGTCCATCACCCGCGAGTCCAGCACCGCCCGCGAGCTGCGCGAGCTGCTCCTG 120
5993R-2.IR_full 121 AGCTCGCGGCGCATCCTCTGCGAGCTGGAGACAGCCGTCAACCAGACGCAGAGTCCGCGC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5993R-2.in silico 121 AGCTCGCGGCGCATCCTCTGCGAGCTGGAGACAGCCGTCAACCAGACGCAGAGTCCGCGC 180
5993R-2.IR_full 181 CAGAAGCAGCGGCGCAGTGGTGCGGCGGTCACAGCAGTTGGCGGCACCACCATGCTGGGT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5993R-2.in silico 181 CAGAAGCAGCGGCGCAGTGGTGCGGCGGTCACAGCAGTTGGCGGCACCACCATGCTGGGT 240
5993R-2.IR_full 241 CAGCAGCTGCCACAGATCTCGCGTCTCGAGATGAACAAGCGTTTGAAGCTGCGCTCCAAG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5993R-2.in silico 241 CAGCAGCTGCCACAGATCTCGCGTCTCGAGATGAACAAGCGTTTGAAGCTGCGCTCCAAG 300
5993R-2.IR_full 301 ACGAGTGGGCCCGGTATGGGTGGTGCTGCTTCCAGTGCATCCATGGCGGCGGGCGAGGCG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5993R-2.in silico 301 ACGAGTGGGCCCGGTATGGGTGGTGCTGCTTCCAGTGCATCCATGGCGGCGGGCGAGGCG 360
5993R-2.IR_full 361 GACTCGATCGATATGCGCTTTGTGAAGCACCACTACTACGACTTTTTGCGCACCATGTAC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5993R-2.in silico 361 GACTCGATCGATATGCGCTTTGTGAAGCACCACTACTACGACTTTTTGCGCACCATGTAC 420
5993R-2.IR_full 421 CAGCTGCTGCGGCGGGATGGCAAGCGGGTAAGGAGTCGGCCACGGAAGCACCACAAGAAG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5993R-2.in silico 421 CAGCTGCTGCGGCGGGATGGCAAGCGGGTAAGGAGTCGGCCACGGAAGCACCACAAGAAG 480
5993R-2.IR_full 481 CAGCAGAGGAGCCAGAAAAAG 501
|||||||||||||||||||||
5993R-2.in silico 481 CAGCAGAGGAGCCAGAAAAAG 501
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_080356.2 |
CG5993-RA (os), mRNA |
| 0.2 |
1 |
3 |
3 |
2 |
NM_135767.1 |
CG5682-RA (CG5682), mRNA |
| 0 |
0 |
3 |
5 |
1 |
NM_176155.1 |
CG33012-RB, transcript variant B (CG33012), mRNA |
| 0 |
0 |
3 |
5 |
1 |
NM_176154.1 |
CG33012-RA, transcript variant A (CG33012), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_135391.2 |
CG13095-RA (CG13095), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_176734.1 |
CG33206-RA, transcript variant A (l(1)G0168), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_176735.1 |
CG33206-RB, transcript variant B (l(1)G0168), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_136202.4 |
CG31678-RA (CG31678), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_143006.1 |
CG13617-RA (CG13617), mRNA |
| 0 |
0 |
2 |
2 |
13 |
NM_167595.3 |
CG12348-RE, transcript variant E (Sh), mRNA |
| 0 |
0 |
2 |
2 |
13 |
NM_206775.1 |
CG12348-RG, transcript variant G (Sh), mRNA |
| 0 |
0 |
2 |
2 |
13 |
NM_078669.3 |
CG12348-RB, transcript variant B (Sh), mRNA |
| 0 |
0 |
2 |
2 |
12 |
NM_206774.1 |
CG12348-RF, transcript variant F (Sh), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_166446.1 |
CG9696-RD, transcript variant D (dom), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_080094.2 |
CG9696-RA, transcript variant A (dom), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_080535.2 |
CG9774-RA (rok), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_139491.1 |
CG1241-RA (Atg2), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_141111.4 |
CG7145-RB, transcript variant B (CG7145), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_168945.3 |
CG7145-RD, transcript variant D (CG7145), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_168942.3 |
CG7145-RA, transcript variant A (CG7145), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_170547.2 |
CG31003-RA (gskt), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_057326.4 |
CG12212-RA, transcript variant A (peb), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_001014722.1 |
CG12212-RB, transcript variant B (peb), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_141731.1 |
CG3996-RA (CG3996), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_132320.2 |
CG12124-RA (CG12124), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168304.1 |
CG5021-RB, transcript variant B (CG5021), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_140013.2 |
CG5021-RA, transcript variant A (CG5021), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_141674.2 |
CG9448-RA (CG9448), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_139458.2 |
CG1317-RB (CG1317), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_136423.1 |
CG11101-RA (pwn), mRNA |
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