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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5952R-3 |
| Symbol |
Fer2 |
Full Name |
48 related 2 |
| CG No |
CG5952 |
Old CG No |
CG5952 |
| Synonyms |
CG5952, Fer2 |
| Accession No (Link to NCBI) |
NM_079652.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCCATTAGCCATTTACCCTTC |
| Primer Seq. 3' |
aatctagaggtaccACATGGACCCTTAGCTCATCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCCNTTAGCC ATTTACCCTT CCACAATTTC GACGATGATC TGATTAACGA TTTGCCCTCC
0061 TGCATTTATG CGGGTCAGCA TCAAGGCGGA GGCGCCACCG GTGGAGGTGG TGGATCGGGA
0121 GGTGGCGGTG GCAGTTCTGG GCTGCGTCTG AGCTCCAACA ACCTGCGTCA CATCCAGCAG
0181 CATTATATGC AACATAGCGG AGAAAATCTG CTCGACTCCT CTACCAATCC AATGGGGGTG
0241 CACAATTCCG GGGGAGGAGC ACCCTTAATG TGCAACAGCC CCAGTGCCAG CAGCAGTGGA
0301 GGAGGGTGCG GCANACGCAG GATCTGCTAN CTCCTGGCGG TGCAGGTGGT CCCAATCCTG
0361 GATATGGATC CGTGGGAGCT GCAACTGCGT CCAGCTACAA AAATGCAGCG ACAGGCGGCC
0421 AATGTGCGGG AAANGGAAAC GCATCCAGAg gtccgcccca actgGTAGCA TCAATTCGGC
0481 TTTCGATGAG CTAAGGGTCC ATGT |
| in silico PCR Fragment |
0001 CCCATTAGCC ATTTACCCTT CCACAATTTC GACGATGATC TGATTAACGA TTTGCCCTCC
0061 TGCATTTATG CGGGTCAGCA TCAAGGCGGA GGCGCCACCG GTGGAGGTGG TGGATCGGGA
0121 GGTGGCGGTG GCAGTTCTGG GCTGCGTCTG AGCTCCAACA ACCTGCGTCA CATCCAGCAG
0181 CATTATATGC AACATAGCGG AGAAAATCTG CTCGACTCCT CTACCAATCC AATGGGGGTG
0241 CACAATTCCG GGGGAGGAGC ACCCTTAATG TGCAACAGCC CCAGTGCCAG CAGCAGTGGA
0301 GGAGGGTGCG GCA-ACGCAG GATCTGCTA- CTCCTGGCGG TGCAGGTGGT CCCAATCCTG
0361 GATATGGATC CGTGGGAGCT GCAACTGCGT CCAGCTAC-A AAATGCAGCG ACAGGCGGCC
0421 AATGTGCGGG AAA-GGAAAC GCATCCAGAG GTCCGCCCCA ACTGGTAGCA TCAATTCGGC
0481 TTTCGATGAG CTAAGGGTCC ATGT |
| Assemble Data |
5952R-3.IR_full 1 CCCNTTAGCCATTTACCCTTCCACAATTTCGACGATGATCTGATTAACGATTTGCCCTCC 60
||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5952R-3.in silico 1 CCCATTAGCCATTTACCCTTCCACAATTTCGACGATGATCTGATTAACGATTTGCCCTCC 60
5952R-3.IR_full 61 TGCATTTATGCGGGTCAGCATCAAGGCGGAGGCGCCACCGGTGGAGGTGGTGGATCGGGA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5952R-3.in silico 61 TGCATTTATGCGGGTCAGCATCAAGGCGGAGGCGCCACCGGTGGAGGTGGTGGATCGGGA 120
5952R-3.IR_full 121 GGTGGCGGTGGCAGTTCTGGGCTGCGTCTGAGCTCCAACAACCTGCGTCACATCCAGCAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5952R-3.in silico 121 GGTGGCGGTGGCAGTTCTGGGCTGCGTCTGAGCTCCAACAACCTGCGTCACATCCAGCAG 180
5952R-3.IR_full 181 CATTATATGCAACATAGCGGAGAAAATCTGCTCGACTCCTCTACCAATCCAATGGGGGTG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5952R-3.in silico 181 CATTATATGCAACATAGCGGAGAAAATCTGCTCGACTCCTCTACCAATCCAATGGGGGTG 240
5952R-3.IR_full 241 CACAATTCCGGGGGAGGAGCACCCTTAATGTGCAACAGCCCCAGTGCCAGCAGCAGTGGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5952R-3.in silico 241 CACAATTCCGGGGGAGGAGCACCCTTAATGTGCAACAGCCCCAGTGCCAGCAGCAGTGGA 300
5952R-3.IR_full 301 GGAGGGTGCGGCANACGCAGGATCTGCTANCTCCTGGCGGTGCAGGTGGTCCCAATCCTG 360
||||||||||||| ||||||||||||||| ||||||||||||||||||||||||||||||
5952R-3.in silico 301 GGAGGGTGCGGCA-ACGCAGGATCTGCTA-CTCCTGGCGGTGCAGGTGGTCCCAATCCTG 360
5952R-3.IR_full 361 GATATGGATCCGTGGGAGCTGCAACTGCGTCCAGCTACAAAAATGCAGCGACAGGCGGCC 420
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
5952R-3.in silico 361 GATATGGATCCGTGGGAGCTGCAACTGCGTCCAGCTAC-AAAATGCAGCGACAGGCGGCC 420
5952R-3.IR_full 421 AATGTGCGGGAAANGGAAACGCATCCAGAGGTCCGCCCCAACTGGTAGCATCAATTCGGC 480
||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
5952R-3.in silico 421 AATGTGCGGGAAA-GGAAACGCATCCAGAGGTCCGCCCCAACTGGTAGCATCAATTCGGC 480
5952R-3.IR_full 481 TTTCGATGAGCTAAGGGTCCATGT 504
||||||||||||||||||||||||
5952R-3.in silico 481 TTTCGATGAGCTAAGGGTCCATGT 504
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079652.1 |
CG5952-RA (Fer2), mRNA |
| 0 |
0 |
4 |
2 |
5 |
NM_080016.2 |
CG4481-RA (Glu-RIB), mRNA |
| 0 |
0 |
3 |
4 |
9 |
NM_167228.1 |
CG32681-RA (CG32681), mRNA |
| 0 |
0 |
2 |
12 |
16 |
NM_167482.3 |
CG32580-RA (CG32580), mRNA |
| 0 |
0 |
2 |
12 |
5 |
NM_057996.3 |
CG7760-RA (cato), mRNA |
| 0 |
0 |
2 |
6 |
7 |
NM_079942.3 |
CG10295-RB, transcript variant B (Pak), mRNA |
| 0 |
0 |
2 |
6 |
7 |
NM_169136.1 |
CG10295-RA, transcript variant A (Pak), mRNA |
| 0 |
0 |
2 |
6 |
7 |
NM_169137.1 |
CG10295-RC, transcript variant C (Pak), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_169845.1 |
CG31043-RA, transcript variant A (gukh), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_176033.1 |
CG18146-RB, transcript variant B (CG18146), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_135851.2 |
CG18146-RA, transcript variant A (CG18146), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_176229.1 |
CG33147-RA (CG33147), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_133114.2 |
CG32541-RA (CG32541), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_140197.1 |
CG18490-RB, transcript variant B (CG18490), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_168453.1 |
CG18490-RC, transcript variant C (CG18490), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_141112.1 |
CG14563-RA (CG14563), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_079107.2 |
CG11173-RA (usnp), mRNA |
| 0 |
0 |
1 |
9 |
13 |
NM_168490.1 |
CG32096-RE, transcript variant E (rols), mRNA |
| 0 |
0 |
1 |
9 |
13 |
NM_140285.2 |
CG32096-RD, transcript variant D (rols), mRNA |
| 0 |
0 |
1 |
9 |
11 |
NM_168489.1 |
CG32096-RC, transcript variant C (rols), mRNA |
| 0 |
0 |
1 |
9 |
11 |
NM_168488.1 |
CG32096-RA, transcript variant A (rols), mRNA |
| 0 |
0 |
1 |
9 |
11 |
NM_168487.1 |
CG32096-RB, transcript variant B (rols), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_170139.1 |
CG31132-RA (BRWD3), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_141867.2 |
CG6962-RA (CG6962), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_133037.2 |
CG7536-RA, transcript variant A (CG7536), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_167599.1 |
CG7536-RB, transcript variant B (CG7536), mRNA |
| 0 |
0 |
1 |
3 |
11 |
NM_132977.1 |
CG12997-RA (CG12997), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_140155.2 |
CG7958-RA, transcript variant A (tna), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_168422.1 |
CG7958-RB, transcript variant B (tna), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_170447.1 |
CG31038-RA, transcript variant A (CG31038), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
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DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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