NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 5924R-3

Symbol CG5924 Full Name CG5924

CG No CG5924 Old CG No CG5924

Synonyms CG5924

Accession No (Link to NCBI) NM_135474.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGAGTGCTCACTAGACCCCAAG

Primer Seq. 3'

aatctagaggtaccCGTCGATCGCCCAGATATAAC

Predicted Fragment Size

IR fragment full Seq

0001 GAGTGCTCAC TAGACCCCAA GGAGTATGTG GACTTCAAGC GGCAGCTGCG GCAGCTCAAC 
0061 TTGCCCCACA AAGACGGACA CACGTGCTTG CAGCTGGAGT GTCGCCTCTG CGATCGCAAT 
0121 AGGCAGCCGG TGACAAACGC CCAAAAGGGC ACCGATCATG GGCTGCTGGC TTACGTAAAC 
0181 AAGCGGACGG GAGCCTTTAT CTGCCCCAAT TGCGACGTAA AAACCTCACT GACCAGCGCT 
0241 TTATTATCCT ATCAGTTGCC CAAGCCGGTT GGCTATAAGC AACCGCTGCA GAGGCAGCCG 
0301 GTCTNATGAA TCCCGATTTC CCCACCTGGC CGTTGTTACT CCCGAGGCCT GTGCAGCTTT 
0361 GGGAATCAAG GGCTTGAAGG AGGACCAACT AAATGCCATA GGAGCACAGT GGGAGCCGCA 
0421 GCAACAGCTG CTCCACTTTA AGTTACGCAA TGCCGCGCAA GTGGAGGTTG GCGAAAAGGT 
0481 GTTATATCTG GGCGATCGAC G

in silico PCR Fragment

0001 GAGTGCTCAC TAGACCCCAA GGAGTATGTG GACTTCAAGC GGCAGCTGCG GCAGCTCAAC 
0061 TTGCCCCACA AAGACGGACA CACGTGCTTG CAGCTGGAGT GTCGCCTCTG CGATCGCAAT 
0121 AGGCAGCCGG TGACAAACGC CCAAAAGGGC ACCGATCATG GGCTGCTGGC TTACGTAAAC 
0181 AAGCGGACGG GAGCCTTTAT CTGCCCCAAT TGCGACGTAA AAACCTCACT GACCAGCGCT 
0241 TTATTATCCT ATCAGTTGCC CAAGCCGGTT GGCTATAAGC AACCGCTGCA GAGGCAGCCG 
0301 GTCT-ATGAA TCCCGATTTC CCCACCTGGC CGTGGTTACT CCCGAGGCCT GTGCAGCTTT 
0361 GGGAATCAAG GGCTTGAAGG AGGACCAACT AAATGCCATA GGAGCACAGT GGGAGCCGCA 
0421 GCAACAGCTG CTCCACTTTA AGTTACGCAA TGCCGCGCAA GTGGAGGTTG GCGAAAAGGT 
0481 GTTATATCTG GGCGATCGAC G

Assemble Data


5924R-3.IR_full       1   GAGTGCTCACTAGACCCCAAGGAGTATGTGGACTTCAAGCGGCAGCTGCGGCAGCTCAAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5924R-3.in silico     1   GAGTGCTCACTAGACCCCAAGGAGTATGTGGACTTCAAGCGGCAGCTGCGGCAGCTCAAC 60


5924R-3.IR_full       61  TTGCCCCACAAAGACGGACACACGTGCTTGCAGCTGGAGTGTCGCCTCTGCGATCGCAAT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5924R-3.in silico     61  TTGCCCCACAAAGACGGACACACGTGCTTGCAGCTGGAGTGTCGCCTCTGCGATCGCAAT 120


5924R-3.IR_full       121 AGGCAGCCGGTGACAAACGCCCAAAAGGGCACCGATCATGGGCTGCTGGCTTACGTAAAC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5924R-3.in silico     121 AGGCAGCCGGTGACAAACGCCCAAAAGGGCACCGATCATGGGCTGCTGGCTTACGTAAAC 180


5924R-3.IR_full       181 AAGCGGACGGGAGCCTTTATCTGCCCCAATTGCGACGTAAAAACCTCACTGACCAGCGCT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5924R-3.in silico     181 AAGCGGACGGGAGCCTTTATCTGCCCCAATTGCGACGTAAAAACCTCACTGACCAGCGCT 240


5924R-3.IR_full       241 TTATTATCCTATCAGTTGCCCAAGCCGGTTGGCTATAAGCAACCGCTGCAGAGGCAGCCG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5924R-3.in silico     241 TTATTATCCTATCAGTTGCCCAAGCCGGTTGGCTATAAGCAACCGCTGCAGAGGCAGCCG 300


5924R-3.IR_full       301 GTCTNATGAATCCCGATTTCCCCACCTGGCCGTTGTTACTCCCGAGGCCTGTGCAGCTTT 360
                          |||| |||||||||||||||||||||||||||| ||||||||||||||||||||||||||
5924R-3.in silico     301 GTCT-ATGAATCCCGATTTCCCCACCTGGCCGTGGTTACTCCCGAGGCCTGTGCAGCTTT 360


5924R-3.IR_full       361 GGGAATCAAGGGCTTGAAGGAGGACCAACTAAATGCCATAGGAGCACAGTGGGAGCCGCA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5924R-3.in silico     361 GGGAATCAAGGGCTTGAAGGAGGACCAACTAAATGCCATAGGAGCACAGTGGGAGCCGCA 420


5924R-3.IR_full       421 GCAACAGCTGCTCCACTTTAAGTTACGCAATGCCGCGCAAGTGGAGGTTGGCGAAAAGGT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5924R-3.in silico     421 GCAACAGCTGCTCCACTTTAAGTTACGCAATGCCGCGCAAGTGGAGGTTGGCGAAAAGGT 480


5924R-3.IR_full       481 GTTATATCTGGGCGATCGACG 501
                          |||||||||||||||||||||
5924R-3.in silico     481 GTTATATCTGGGCGATCGACG 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_135474.1	CG5924-RA (CG5924), mRNA
0	0	2	6	9	NM_001043265.1	CG34139-RA (CG34139), mRNA
0	0	2	5	13	NM_135274.2	CG5261-RB, transcript variant B (CG5261), mRNA
0	0	2	5	13	NM_164749.1	CG5261-RA, transcript variant A (CG5261), mRNA
0	0	2	4	7	NM_170132.1	CG31137-RD, transcript variant D (twin), mRNA
0	0	2	3	14	NM_168413.1	CG32062-RB, transcript variant B (CG32062), mRNA
0	0	2	3	14	NM_168412.1	CG32062-RD, transcript variant D (CG32062), mRNA
0	0	2	3	3	NM_168634.3	CG6169-RB, transcript variant B (CG6169), mRNA
0	0	2	3	3	NM_140548.3	CG6169-RA, transcript variant A (CG6169), mRNA
0	0	2	3	3	NM_206378.1	CG6169-RC, transcript variant C (CG6169), mRNA
0	0	2	3	3	NM_001014585.1	CG6169-RD, transcript variant D (CG6169), mRNA
0	0	2	2	7	NM_136724.1	CG12911-RA (CG12911), mRNA
0	0	2	2	4	NM_135083.2	CG6634-RA (mid), mRNA
0	0	2	2	3	NM_142299.1	CG11769-RA (CG11769), mRNA
0	0	1	14	20	NM_169694.1	CG3992-RB, transcript variant B (srp), mRNA
0	0	1	14	20	NM_169696.1	CG3992-RA, transcript variant A (srp), mRNA
0	0	1	14	16	NM_001032019.1	CG3992-RD, transcript variant D (srp), mRNA
0	0	1	8	9	NM_142786.1	CG13847-RA (CG13847), mRNA
0	0	1	8	3	NM_164707.1	CG11199-RB, transcript variant B (Liprin-alpha), mRNA
0	0	1	8	3	NM_135223.2	CG11199-RA, transcript variant A (Liprin-alpha), mRNA
0	0	1	7	3	NM_132051.1	CG3726-RA (CG3726), mRNA
0	0	1	7	2	NM_137653.2	CG11175-RA (CG11175), mRNA
0	0	1	6	16	NM_167189.2	CG32705-RA (CG32705), mRNA
0	0	1	6	8	NM_166559.1	CG3622-RA, transcript variant A (CG3622), mRNA
0	0	1	6	8	NM_137874.2	CG3622-RB, transcript variant B (CG3622), mRNA
0	0	1	4	15	NM_206303.1	CG6964-RD, transcript variant D (Gug), mRNA
0	0	1	4	15	NM_176300.1	CG6964-RC, transcript variant C (Gug), mRNA
0	0	1	4	15	NM_079249.2	CG6964-RB, transcript variant B (Gug), mRNA
0	0	1	4	15	NM_145106.1	CG6964-RA, transcript variant A (Gug), mRNA
0	0	1	4	14	NM_135715.2	CG31761-RA, transcript variant A (bru-2), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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