Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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NIG-RNAi Strains Detail |
Request : Click the "Order" button to request for this stock |
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
Stock ID |
5896R-2 |
Symbol |
grass |
Full Name |
Gram-positive Specific Serine protease |
CG No |
CG5896 |
Old CG No |
CG5896 |
Synonyms |
grass, CG5896, c-SP1, SP1, SP2 |
Accession No (Link to NCBI) |
NM_170318.1 |
Inserted Chr. |
lll |
Insertional Mutation |
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Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
Map Viewer |
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Reference
[Please submit your publication]
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Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T. RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc. Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Kambris Z, Brun S, Jang IH, Nam HJ, Romeo Y, Takahashi K, Lee WJ, Ueda R, Lemaitre B. Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation. Curr Biol (2006) 16(8) 808-13
[
PubMed ID = 16631589
]
[
RRC reference
]
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Comment |
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Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
Primer Seq. 5' |
aaggcctacatggccggaccgCCAGTTACCTGCAGAAGGCAC |
Primer Seq. 3' |
aatctagaggtaccCTGGAAGGGGACGCTCCTGTT |
Predicted Fragment Size |
500 |
IR fragment full Seq |
0001 CCAGTTACCT GCAGAAGGCA CTCTGCGGCG AGTTCAATGG AGTGCGCCAC TTCTGCTGCC
0061 CCTCAGCCAA CATTCAGCAC AACTCCAAGG TGATGAGCCT GTTCAAGGAC GAAAACTTCG
0121 ACTGCGGCAA CTTCCTCAGC CAAAGAGTGT CCAATGGCTA CGAGGTGAAG CTCTCCTCCA
0181 GACCCTGGAT GGCCCTGCTC CGTTATCAGC AGTTTGGAGA GTCCAGATTC CTTTGCGGCG
0241 GAGCTATGAT ATCCGAGCGC TACATCCTGA CTGCTGCTCA CTGTGTCCAC GGTCTTCAGA
0301 ACGATCTTTA TGAGATCCGG CTCGGCGAGC ACCGCATCTC TACGGAGGAG GATTGCCGGC
0361 AGCAGGGACG CAAGAAGAAA TGTGCCCCGC CAGTTGTGAA TGTGGGCATT GAGAAGCATC
0421 TGATTCATGA GAAGTACGAC GCCCGCCACA TCATGCATGA CATAGCTCTC CTGAAGCTCA
0481 ACAGGAGCGT CCCCTTCCAG |
in silico PCR Fragment |
0001 CCAGTTACCT GCAGAAGGCA CTCTGCGGCG AGTTCAATGG AGTGCGCCAC TTCTGCTGCC
0061 CCTCAGCCAA CATTCAGCAC AACTCCAAGG TGATGAGCCT GTTCAAGGAC GAAAACTTTG
0121 ACTGCGGCAA CTTCCTCAGC CAAAGAGTGT CCAATGGCTA CGAGGTGAAG CTCTCCTCCA
0181 GACCCTGGAT GGCCCTGCTC CGTTATCAGC AGTTTGGAGA GTCCAGATTC CTTTGCGGCG
0241 GAGCTATGAT ATCCGAGCGC TACATCCTGA CTGCTGCTCA CTGTGTCCAC GGTCTTCAGA
0301 ACGATCTTTA TGAGATCCGG CTCGGCGAGC ACCGCATCTC TACGGAGGAG GATTGCCGGC
0361 AGCAGGGACG CAAGAAGAAA TGTGCCCCGC CAGTTGTGAA TGTGGGCATT GAGAAGCATC
0421 TGATTCATGA GAAGTACGAC GCCCGCCACA TCATGCATGA CATAGCTCTC CTGAAGCTCA
0481 ACAGGAGCGT CCCCTTCCAG |
Assemble Data |
5896R-2.IR_full 1 CCAGTTACCTGCAGAAGGCACTCTGCGGCGAGTTCAATGGAGTGCGCCACTTCTGCTGCC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5896R-2.in silico 1 CCAGTTACCTGCAGAAGGCACTCTGCGGCGAGTTCAATGGAGTGCGCCACTTCTGCTGCC 60
5896R-2.IR_full 61 CCTCAGCCAACATTCAGCACAACTCCAAGGTGATGAGCCTGTTCAAGGACGAAAACTTCG 120
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
5896R-2.in silico 61 CCTCAGCCAACATTCAGCACAACTCCAAGGTGATGAGCCTGTTCAAGGACGAAAACTTTG 120
5896R-2.IR_full 121 ACTGCGGCAACTTCCTCAGCCAAAGAGTGTCCAATGGCTACGAGGTGAAGCTCTCCTCCA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5896R-2.in silico 121 ACTGCGGCAACTTCCTCAGCCAAAGAGTGTCCAATGGCTACGAGGTGAAGCTCTCCTCCA 180
5896R-2.IR_full 181 GACCCTGGATGGCCCTGCTCCGTTATCAGCAGTTTGGAGAGTCCAGATTCCTTTGCGGCG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5896R-2.in silico 181 GACCCTGGATGGCCCTGCTCCGTTATCAGCAGTTTGGAGAGTCCAGATTCCTTTGCGGCG 240
5896R-2.IR_full 241 GAGCTATGATATCCGAGCGCTACATCCTGACTGCTGCTCACTGTGTCCACGGTCTTCAGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5896R-2.in silico 241 GAGCTATGATATCCGAGCGCTACATCCTGACTGCTGCTCACTGTGTCCACGGTCTTCAGA 300
5896R-2.IR_full 301 ACGATCTTTATGAGATCCGGCTCGGCGAGCACCGCATCTCTACGGAGGAGGATTGCCGGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5896R-2.in silico 301 ACGATCTTTATGAGATCCGGCTCGGCGAGCACCGCATCTCTACGGAGGAGGATTGCCGGC 360
5896R-2.IR_full 361 AGCAGGGACGCAAGAAGAAATGTGCCCCGCCAGTTGTGAATGTGGGCATTGAGAAGCATC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5896R-2.in silico 361 AGCAGGGACGCAAGAAGAAATGTGCCCCGCCAGTTGTGAATGTGGGCATTGAGAAGCATC 420
5896R-2.IR_full 421 TGATTCATGAGAAGTACGACGCCCGCCACATCATGCATGACATAGCTCTCCTGAAGCTCA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5896R-2.in silico 421 TGATTCATGAGAAGTACGACGCCCGCCACATCATGCATGACATAGCTCTCCTGAAGCTCA 480
5896R-2.IR_full 481 ACAGGAGCGTCCCCTTCCAG 500
||||||||||||||||||||
5896R-2.in silico 481 ACAGGAGCGTCCCCTTCCAG 500
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Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
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cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
100 |
482 |
0 |
0 |
0 |
NM_170318.1 |
CG5896-RA, transcript variant A (CG5896), mRNA |
100 |
482 |
0 |
0 |
0 |
NM_143286.1 |
CG5896-RB, transcript variant B (CG5896), mRNA |
0.2 |
1 |
7 |
2 |
4 |
NM_166398.1 |
CG18065-RA, transcript variant A (CG18065), mRNA |
0.2 |
1 |
7 |
2 |
4 |
NM_001032270.1 |
CG13430-RB, transcript variant B (CG13430), mRNA |
0.2 |
1 |
7 |
2 |
4 |
NM_001032271.1 |
CG13430-RA, transcript variant A (CG13430), mRNA |
0.2 |
1 |
2 |
2 |
4 |
NM_078753.2 |
CG8867-RA, transcript variant A (Jon25Bi), mRNA |
0.2 |
1 |
2 |
2 |
4 |
NM_001038784.1 |
CG8867-RB, transcript variant B (Jon25Bi), mRNA |
0 |
0 |
3 |
4 |
13 |
NM_176575.1 |
CG33103-RA, transcript variant A (Ppn), mRNA |
0 |
0 |
3 |
4 |
12 |
NM_176574.1 |
CG33103-RB, transcript variant B (Ppn), mRNA |
0 |
0 |
3 |
3 |
7 |
NM_079220.1 |
CG6457-RA (yip7), mRNA |
0 |
0 |
3 |
3 |
3 |
NM_206275.2 |
CG12605-RA, transcript variant A (CG12605), mRNA |
0 |
0 |
3 |
3 |
3 |
NM_206274.2 |
CG12605-RC, transcript variant C (CG12605), mRNA |
0 |
0 |
3 |
3 |
3 |
NM_139588.3 |
CG12605-RB, transcript variant B (CG12605), mRNA |
0 |
0 |
2 |
2 |
3 |
NM_139755.2 |
CG6467-RA (Jon65Aiv), mRNA |
0 |
0 |
1 |
4 |
6 |
NM_164704.1 |
CG11326-RE, transcript variant E (Tsp), mRNA |
0 |
0 |
1 |
4 |
6 |
NM_164702.1 |
CG11326-RC, transcript variant C (Tsp), mRNA |
0 |
0 |
1 |
4 |
6 |
NM_164703.1 |
CG11326-RD, transcript variant D (Tsp), mRNA |
0 |
0 |
1 |
4 |
3 |
NM_167231.3 |
CG17255-RB, transcript variant B (CG17255), mRNA |
0 |
0 |
1 |
4 |
3 |
NM_132403.3 |
CG17255-RA, transcript variant A (CG17255), mRNA |
0 |
0 |
1 |
3 |
6 |
NM_001014526.1 |
CG33528-RE, transcript variant E (CG33528), mRNA |
0 |
0 |
1 |
3 |
4 |
NM_001014524.1 |
CG33528-RC, transcript variant C (CG33528), mRNA |
0 |
0 |
1 |
3 |
4 |
NM_001014525.1 |
CG33528-RD, transcript variant D (CG33528), mRNA |
0 |
0 |
1 |
3 |
3 |
NM_139504.2 |
CG1893-RA (CG1893), mRNA |
0 |
0 |
1 |
3 |
2 |
NM_143408.2 |
CG1401-RA (cul-5), mRNA |
0 |
0 |
1 |
2 |
4 |
NM_166925.1 |
CG4199-RA, transcript variant A (CG4199), mRNA |
0 |
0 |
1 |
2 |
4 |
NM_134532.2 |
CG9581-RA (CG9581), mRNA |
0 |
0 |
1 |
2 |
4 |
NM_166922.1 |
CG4199-RB, transcript variant B (CG4199), mRNA |
0 |
0 |
1 |
2 |
4 |
NM_130620.2 |
CG4199-RD, transcript variant D (CG4199), mRNA |
0 |
0 |
1 |
2 |
4 |
NM_166923.1 |
CG4199-RC, transcript variant C (CG4199), mRNA |
0 |
0 |
1 |
2 |
4 |
NM_166921.1 |
CG4199-RE, transcript variant E (CG4199), mRNA |
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Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
N |
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+ |
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DB |
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+ |
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DC |
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++ |
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DM |
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+ |
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VM |
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+- |
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VB |
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Caspase-3 signal
N |
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++ |
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DB |
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++ |
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DC |
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++ |
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DM |
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++ |
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VM |
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+ |
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VB |
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++ |
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Dcompartment |
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Penetrance |
++ |
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