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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 5895R-2 
 Symbol CG5895  Full Name CG5895 
 CG No CG5895  Old CG No CG5895 
 Synonyms CG5895 
 Accession No (Link to NCBI) NM_140571.3 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTTGATTGGCTCCCTTGAAGCG 
 Primer Seq. 3' 
aatctagaggtaccCTGACCAGTATCGAATTGGAC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TTGATTGGCT CCCTTGAAGC GGGCTGTCCT GTTTACCTAA CCATTTCGCT GACGGGCAAA 
0061 ACCCTGCAGG ACACCTTTCT CGAGATCAAC TGGGGCCCGA ACTGCTATGG ACCACCCGAG 
0121 TGGGTGGGTA TCTACAGCCA GGATCCGGCC ATCTCCAACT TTCAGCCAGA TCTGCGCATC 
0181 AATGGTATTT TCAATATAAC CGGCAAGATG CTGACGCCCA TCANAGCTGG GCAAGCTTCG 
0241 TTTTCCCGGT GGCTGGAATA AAAAAGATAG TAGCGATCAG CCGGCAACGC AATATCTCAN 
0301 AGGGCAANGT GCCTGCCACA CTATGTGGCC AGCTACAATG GCACCGAGCT GCTCACGGTG 
0361 GATTGCCTTA AGATACAGCC CAATTGGATG GGCCAGATCA AGGACATCAA TCGAATGGCG 
0421 CTGAAGGACA TCTTNCCTGC CCGGCACACA TGCCTCGGCG GCCGTCTTGG GCAGCTCCAG 
0481 TAAGTCCAAT TCGATACTGG TCAG 
 in silico PCR Fragment 
0001 TTGATTGGCT CCCTTGAAGC GGGCTGTCCT GTTTACCTAA CCATTTCGCT GACGGGCAAA 
0061 ACCCTGCAGG ACACCTTTCT CGAGATCAAC TGGGGCCCGA ACTGCTATGG ACCACCCGAG 
0121 TGGGTGGGTA TCTACAGCCA GGATCCGGCC ATCTCCAACT TTCAGCCAGA TCTGCGCATC 
0181 AATGGTATTT TCAATATAAC CGGCAAAATG CAGACGCCCA TCA-AGCTGG GCAAGCTTCG 
0241 TTTTCCCGGT GGCTGGAACA AAAAAGATAG TAGCGATCAG CCGGCAACGC AATATCTCA- 
0301 AGGGCAA-GT GCCTGCCACA CTATGTGGCC AGCTACAATG GCACCGAGCT GCTCACGGTG 
0361 GATTGCCTTA AGATACAGCC CAATTGGATG GGCCAGATCA AGGACATCAA TCGAATGGCG 
0421 CTGAAGGATA TCTT-CCTGC CCGGCACACA TGCCTCGGCG GCCGTCTTGG GCAGCTCCAG 
0481 TAAGTCCAAT TCGATACTGG TCAG 
 Assemble Data 

5895R-2.IR_full       1   TTGATTGGCTCCCTTGAAGCGGGCTGTCCTGTTTACCTAACCATTTCGCTGACGGGCAAA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5895R-2.in silico     1   TTGATTGGCTCCCTTGAAGCGGGCTGTCCTGTTTACCTAACCATTTCGCTGACGGGCAAA 60


5895R-2.IR_full       61  ACCCTGCAGGACACCTTTCTCGAGATCAACTGGGGCCCGAACTGCTATGGACCACCCGAG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5895R-2.in silico     61  ACCCTGCAGGACACCTTTCTCGAGATCAACTGGGGCCCGAACTGCTATGGACCACCCGAG 120


5895R-2.IR_full       121 TGGGTGGGTATCTACAGCCAGGATCCGGCCATCTCCAACTTTCAGCCAGATCTGCGCATC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5895R-2.in silico     121 TGGGTGGGTATCTACAGCCAGGATCCGGCCATCTCCAACTTTCAGCCAGATCTGCGCATC 180


5895R-2.IR_full       181 AATGGTATTTTCAATATAACCGGCAAGATGCTGACGCCCATCANAGCTGGGCAAGCTTCG 240
                          |||||||||||||||||||||||||| |||| ||||||||||| ||||||||||||||||
5895R-2.in silico     181 AATGGTATTTTCAATATAACCGGCAAAATGCAGACGCCCATCA-AGCTGGGCAAGCTTCG 240


5895R-2.IR_full       241 TTTTCCCGGTGGCTGGAATAAAAAAGATAGTAGCGATCAGCCGGCAACGCAATATCTCAN 300
                          |||||||||||||||||| |||||||||||||||||||||||||||||||||||||||| 
5895R-2.in silico     241 TTTTCCCGGTGGCTGGAACAAAAAAGATAGTAGCGATCAGCCGGCAACGCAATATCTCA- 300


5895R-2.IR_full       301 AGGGCAANGTGCCTGCCACACTATGTGGCCAGCTACAATGGCACCGAGCTGCTCACGGTG 360
                          ||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
5895R-2.in silico     301 AGGGCAA-GTGCCTGCCACACTATGTGGCCAGCTACAATGGCACCGAGCTGCTCACGGTG 360


5895R-2.IR_full       361 GATTGCCTTAAGATACAGCCCAATTGGATGGGCCAGATCAAGGACATCAATCGAATGGCG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5895R-2.in silico     361 GATTGCCTTAAGATACAGCCCAATTGGATGGGCCAGATCAAGGACATCAATCGAATGGCG 420


5895R-2.IR_full       421 CTGAAGGACATCTTNCCTGCCCGGCACACATGCCTCGGCGGCCGTCTTGGGCAGCTCCAG 480
                          |||||||| ||||| |||||||||||||||||||||||||||||||||||||||||||||
5895R-2.in silico     421 CTGAAGGATATCTT-CCTGCCCGGCACACATGCCTCGGCGGCCGTCTTGGGCAGCTCCAG 480


5895R-2.IR_full       481 TAAGTCCAATTCGATACTGGTCAG 504
                          ||||||||||||||||||||||||
5895R-2.in silico     481 TAAGTCCAATTCGATACTGGTCAG 504



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_140571.3  CG5895-RA (CG5895), mRNA 
0   0  4  2  3  NM_135659.2  CG6488-RA (CG6488), mRNA 
0   0  2  7  3  NM_079355.2  CG6988-RA, transcript variant A (Pdi), mRNA 
0   0  2  7  3  NM_168614.2  CG6988-RD, transcript variant D (Pdi), mRNA 
0   0  2  2  3  NM_080329.2  CG3697-RA (mei-9), mRNA 
0   0  2  2  2  NM_143818.1  CG10240-RA (Cyp6a22), mRNA 
0   0  1  5  6  NM_139850.3  CG8596-RA (CG8596), mRNA 
0   0  1  5  1  NM_132955.1  CG4937-RA, transcript variant A (RhoGAP15B), mRNA 
0   0  1  5  1  NM_001042817.1  CG4937-RB, transcript variant B (RhoGAP15B), mRNA 
0   0  1  4  2  NM_176461.1  CG17309-RE, transcript variant E (Csk), mRNA 
0   0  1  4  2  NM_169415.2  CG17309-RC, transcript variant C (Csk), mRNA 
0   0  1  4  2  NM_169416.2  CG17309-RD, transcript variant D (Csk), mRNA 
0   0  1  4  2  NM_141840.1  CG17309-RA, transcript variant A (Csk), mRNA 
0   0  1  4  2  NM_169414.1  CG17309-RB, transcript variant B (Csk), mRNA 
0   0  1  3  7  NM_206339.1  CG11658-RB, transcript variant B (CG11658), mRNA 
0   0  1  3  7  NM_206338.1  CG11658-RC, transcript variant C (CG11658), mRNA 
0   0  1  3  7  NM_140241.2  CG11658-RA, transcript variant A (CG11658), mRNA 
0   0  1  3  7  NM_206336.1  CG11658-RE, transcript variant E (CG11658), mRNA 
0   0  1  3  7  NM_206337.1  CG11658-RD, transcript variant D (CG11658), mRNA 
0   0  1  3  6  NM_143025.1  CG13632-RA (CG13632), mRNA 
0   0  1  2  5  NM_142396.1  CG7397-RA (CG7397), mRNA 
0   0  1  2  4  NM_136202.4  CG31678-RA (CG31678), mRNA 
0   0  1  2  2  NM_142997.1  CG17784-RA (CG17784), mRNA 
0   0  0  6  7  NM_140423.2  CG8783-RA, transcript variant A (CG8783), mRNA 
0   0  0  6  7  NM_168570.1  CG8783-RB, transcript variant B (CG8783), mRNA 
0   0  0  6  6  NM_134804.3  CG7261-RA (CG7261), mRNA 
0   0  0  5  4  NM_132943.1  CG13004-RA (CG13004), mRNA 
0   0  0  5  4  NM_137197.1  CG12960-RA (CG12960), mRNA 
0   0  0  5  3  NM_176539.1  CG33110-RA (CG33110), mRNA 
0   0  0  4  7  NM_136455.1  CG2093-RA (CG2093), mRNA 

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