NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 5883R-1

Symbol CG5883 Full Name CG5883

CG No CG5883 Old CG No CG5883

Synonyms CG5883

Accession No (Link to NCBI) NM_140269.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGCGTCCGCGGATACTTACTG

Primer Seq. 3'

aatctagaggtaccCGGGGATACCGGATCCCAAAT

Predicted Fragment Size

IR fragment full Seq

0001 AGCGTCCgCG NATACTTACT GCGATACAAG CAAAATATGC AAGGGATCTG GAACGGGCTA 
0061 TATTGGTGAT ACCATAAATT GTGCCAACTG GTATTACTGT GATGCGGATG CGTTGTTGGG 
0121 CAAAGGAACA TGCAACCTGG GAATGTACTT CGATCAAGTA AGCAAGAGTT GCGTTTATTC 
0181 GGAGGACACA GTTTGTGCCG CCAAATATGA GATCTGCGAC GTTGCGCCCG TAGGCACACC 
0241 ATTCCGCGAT GATGCCAACT GCCACAAGTA CTATACCTGC AGCAGTAAGA GCCTGGTGGA 
0301 AAATACATGC GAAAATGGAC TGTATTACAA CGTGGCCACC GGAACTTGTG TGCAGGAAAA 
0361 AGGACGTTAT CTGCGAGAAC CATCCGTTGC CAGATGAGGT GTGTGGCAAC AAGAAGCTgG 
0421 NGGTGCGCAA CAAATTCGTT TCAGATATGG CCACATGTCG CGGATATTAC TACTGCCGCG 
0481 ATTTGGGATC CGGTATCCCC G

in silico PCR Fragment

0001 AGCGTCCGCG GATACTTACT GCGATACAAG CAAAATATGC AAGGGATCTG GAACGGGCTA 
0061 TATTGGTGAT ACCATAAATT GTGCCAACTG GTATTACTGT GATGCGGATG CGTTGTTGGG 
0121 CAAAGGAACA TGCAACCTGG GAATGTACTT CGATCAAGTA AGCAAGAGTT GCGTTTATTC 
0181 GGAGGACACA GTTTGTGCCG CCAAATATGA GATCTGCGAC GTTGCGCCCG TAGGCACACC 
0241 ATTCCGCGAT GATGCCAACT GCCACAAGTA CTATACCTGC AGCAGTAAGA GCCTGGTGGA 
0301 AAATACATGC GAAAATGGAC TGTATTACAA CGTGGCCACC GGAACTTGTG TG-AGGAAAA 
0361 AGGACGTTAT CTGCGAGAAC CATCCGTTGC CAGATGAGGT GTGTGGCAAC AAGAAGCTGG 
0421 CGGTGCGCAA CAAATTCGTT TCAGATATGG CCACATGTCG CGGATATTAC TACTGCCGCG 
0481 ATTTGGGATC CGGTATCCCC G

Assemble Data


5883R-1.IR_full       1   AGCGTCCGCGNATACTTACTGCGATACAAGCAAAATATGCAAGGGATCTGGAACGGGCTA 60
                          |||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
5883R-1.in silico     1   AGCGTCCGCGGATACTTACTGCGATACAAGCAAAATATGCAAGGGATCTGGAACGGGCTA 60


5883R-1.IR_full       61  TATTGGTGATACCATAAATTGTGCCAACTGGTATTACTGTGATGCGGATGCGTTGTTGGG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5883R-1.in silico     61  TATTGGTGATACCATAAATTGTGCCAACTGGTATTACTGTGATGCGGATGCGTTGTTGGG 120


5883R-1.IR_full       121 CAAAGGAACATGCAACCTGGGAATGTACTTCGATCAAGTAAGCAAGAGTTGCGTTTATTC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5883R-1.in silico     121 CAAAGGAACATGCAACCTGGGAATGTACTTCGATCAAGTAAGCAAGAGTTGCGTTTATTC 180


5883R-1.IR_full       181 GGAGGACACAGTTTGTGCCGCCAAATATGAGATCTGCGACGTTGCGCCCGTAGGCACACC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5883R-1.in silico     181 GGAGGACACAGTTTGTGCCGCCAAATATGAGATCTGCGACGTTGCGCCCGTAGGCACACC 240


5883R-1.IR_full       241 ATTCCGCGATGATGCCAACTGCCACAAGTACTATACCTGCAGCAGTAAGAGCCTGGTGGA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5883R-1.in silico     241 ATTCCGCGATGATGCCAACTGCCACAAGTACTATACCTGCAGCAGTAAGAGCCTGGTGGA 300


5883R-1.IR_full       301 AAATACATGCGAAAATGGACTGTATTACAACGTGGCCACCGGAACTTGTGTGCAGGAAAA 360
                          |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
5883R-1.in silico     301 AAATACATGCGAAAATGGACTGTATTACAACGTGGCCACCGGAACTTGTGTG-AGGAAAA 360


5883R-1.IR_full       361 AGGACGTTATCTGCGAGAACCATCCGTTGCCAGATGAGGTGTGTGGCAACAAGAAGCTGG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5883R-1.in silico     361 AGGACGTTATCTGCGAGAACCATCCGTTGCCAGATGAGGTGTGTGGCAACAAGAAGCTGG 420


5883R-1.IR_full        421 GGTGCGCAACAAATTCGTTTCAGATATGGCCACATGTCGCGGATATTACTACTGCCGCG 479
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5883R-1.in silico      421 GGTGCGCAACAAATTCGTTTCAGATATGGCCACATGTCGCGGATATTACTACTGCCGCG 479


5883R-1.IR_full       481 ATTTGGGATCCGGTATCCCCG 500
                          |||||||||||||||||||||
5883R-1.in silico     481 ATTTGGGATCCGGTATCCCCG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140269.2	CG5883-RA (CG5883), mRNA
0	0	6	8	11	NM_140264.1	CG5897-RA (CG5897), mRNA
0	0	1	4	3	NM_135134.2	CG9092-RA (Gal), mRNA
0	0	1	4	3	NM_130617.2	CG4281-RA (CG4281), mRNA
0	0	1	4	2	NM_134486.1	CG14221-RA (CG14221), mRNA
0	0	1	3	5	NM_132876.3	CG9214-RA, transcript variant A (Tob), mRNA
0	0	1	3	5	NM_206760.1	CG9214-RB, transcript variant B (Tob), mRNA
0	0	1	3	2	NM_078582.3	CG32659-RA (Ten-a), mRNA
0	0	1	2	3	NM_001014638.1	CG33541-RA (CG33541), mRNA
0	0	0	5	7	NM_164887.1	CG31714-RA (CG31714), mRNA
0	0	0	4	6	NM_169580.1	CG8524-RB, transcript variant B (NK7.1), mRNA
0	0	0	4	6	NM_142100.2	CG8524-RA, transcript variant A (NK7.1), mRNA
0	0	0	4	3	NM_132633.2	CG4332-RA (CG4332), mRNA
0	0	0	4	2	NM_132205.2	CG2233-RA (CG2233), mRNA
0	0	0	4	2	NM_136446.2	CG1603-RA (CG1603), mRNA
0	0	0	3	10	NM_176445.1	CG33208-RC, transcript variant C (MICAL), mRNA
0	0	0	3	8	NM_176447.1	CG33208-RF, transcript variant F (MICAL), mRNA
0	0	0	3	8	NM_176449.1	CG33208-RH, transcript variant H (MICAL), mRNA
0	0	0	3	8	NM_176446.1	CG33208-RD, transcript variant D (MICAL), mRNA
0	0	0	3	8	NM_176448.1	CG33208-RG, transcript variant G (MICAL), mRNA
0	0	0	3	7	NM_167794.1	CG1210-RE, transcript variant E (Pk61C), mRNA
0	0	0	3	7	NM_167793.1	CG1210-RB, transcript variant B (Pk61C), mRNA
0	0	0	3	7	NM_167795.1	CG1210-RG, transcript variant G (Pk61C), mRNA
0	0	0	3	7	NM_167789.1	CG1210-RC, transcript variant C (Pk61C), mRNA
0	0	0	3	7	NM_167791.1	CG1210-RF, transcript variant F (Pk61C), mRNA
0	0	0	3	7	NM_167792.1	CG1210-RH, transcript variant H (Pk61C), mRNA
0	0	0	3	7	NM_080382.2	CG1210-RA, transcript variant A (Pk61C), mRNA
0	0	0	3	7	NM_167790.1	CG1210-RD, transcript variant D (Pk61C), mRNA
0	0	0	3	5	NM_057672.2	CG1429-RD, transcript variant D (Mef2), mRNA
0	0	0	3	5	NM_057670.2	CG1429-RC, transcript variant C (Mef2), mRNA

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