NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 5853R-1

Symbol CG5853 Full Name CG5853

CG No CG5853 Old CG No CG5853

Synonyms CG5853

Accession No (Link to NCBI) NM_176005.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAGTTCTTGTTCGGCAGCGAC

Primer Seq. 3'

aatctagaggtaccCGACCATTGAGCAAGATGGAG

Predicted Fragment Size

IR fragment full Seq

0001 CAGTTCTTGT TCGGCAGCGA CAACGGAGTg GCCGGCCTCG GTCTGGGCAT CGAGATCGGG 
0061 GAGGTGACCT CCAGTCGCGT CTATAACAGC ACCACAGTgC CAGTTGGCAA CAGTGGCGGA 
0121 TGGCGGAATG CCCAGTCCAG TGTTACGCCA CCACCCATAC CCACACCGCC ACAGGTGAAC 
0181 TATACCAATG GGAATGCGCC CAGCAAGCAG CCGCCACTGG AACCTGTCGT GGAGGAGGAG 
0241 GTGCACTTCG ACACCGATGC CCTCAATAAT CTGCCAGCTC GCGAGCCTGT GGACATGGAG 
0301 TTCAAGGAGC TGTCCCTCAC CGTCAAATTG GGCTTCAATC GAGGATCCAA GGAAATCTTG 
0361 CACAATGTGT GCGGAAAGTT CCCAGGCAGC CAGCTGATCG CCATCATGGG AcCTTCGGGT 
0421 GCAGGAAAGT CCACTTTGCT AGATGCCCTT TCAGGTTTCA AGACGACCGG AGTGGATGGC 
0481 TCCATCTTGC TCAATGGTCG

in silico PCR Fragment

0001 CAGTTCTTGT TCGGCAGCGA CAACGGAGTG GCCGGCCTCG GTCTGGGCAT CGAGATCGGG 
0061 GAGGTGACCT CCAGTCGCGT CTATAACAGC ACCACAGTGC CAGTTGGCAA CAGTGGCGGA 
0121 TGGCGGAATG CCCAGTCCAG TGTTACGCCA CCACCCATAC CCACACCGCC ACAGGTGAAC 
0181 TATACCAATG GGAATGAGCC CAGCAAGCAG CCGCCACTGG AACCTGTCGT GGAGGAGGAG 
0241 GTGCACTTCG ACACCGATGC CCTCAATAAT CTGCCAGCTC GCGAGCCTGT GGACATGGAG 
0301 TTCAAGGAGC TGTCCCTCAC CGTCAAATTG GGCTTCAATC GAGGATCCAA GGAAATCTTG 
0361 CACAATGTGT GCGGAAAGTT CCCAGGCAGC CAGCTGATTG CCATCATGGG ACCTTCGGGT 
0421 GCAGGAAAGT CCACTTTGCT AGATGCCCTT TCAGGTTTCA AGACGACCGG AGTGGATGGC 
0481 TCCATCTTGC TCAATGGTCG

Assemble Data


5853R-1.IR_full       1   CAGTTCTTGTTCGGCAGCGACAACGGAGTGGCCGGCCTCGGTCTGGGCATCGAGATCGGG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5853R-1.in silico     1   CAGTTCTTGTTCGGCAGCGACAACGGAGTGGCCGGCCTCGGTCTGGGCATCGAGATCGGG 60


5853R-1.IR_full       61  GAGGTGACCTCCAGTCGCGTCTATAACAGCACCACAGTGCCAGTTGGCAACAGTGGCGGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5853R-1.in silico     61  GAGGTGACCTCCAGTCGCGTCTATAACAGCACCACAGTGCCAGTTGGCAACAGTGGCGGA 120


5853R-1.IR_full       121 TGGCGGAATGCCCAGTCCAGTGTTACGCCACCACCCATACCCACACCGCCACAGGTGAAC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5853R-1.in silico     121 TGGCGGAATGCCCAGTCCAGTGTTACGCCACCACCCATACCCACACCGCCACAGGTGAAC 180


5853R-1.IR_full       181 TATACCAATGGGAATGCGCCCAGCAAGCAGCCGCCACTGGAACCTGTCGTGGAGGAGGAG 240
                          |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
5853R-1.in silico     181 TATACCAATGGGAATGAGCCCAGCAAGCAGCCGCCACTGGAACCTGTCGTGGAGGAGGAG 240


5853R-1.IR_full       241 GTGCACTTCGACACCGATGCCCTCAATAATCTGCCAGCTCGCGAGCCTGTGGACATGGAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5853R-1.in silico     241 GTGCACTTCGACACCGATGCCCTCAATAATCTGCCAGCTCGCGAGCCTGTGGACATGGAG 300


5853R-1.IR_full       301 TTCAAGGAGCTGTCCCTCACCGTCAAATTGGGCTTCAATCGAGGATCCAAGGAAATCTTG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5853R-1.in silico     301 TTCAAGGAGCTGTCCCTCACCGTCAAATTGGGCTTCAATCGAGGATCCAAGGAAATCTTG 360


5853R-1.IR_full       361 CACAATGTGTGCGGAAAGTTCCCAGGCAGCCAGCTGATCGCCATCATGGGACCTTCGGGT 420
                          |||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
5853R-1.in silico     361 CACAATGTGTGCGGAAAGTTCCCAGGCAGCCAGCTGATTGCCATCATGGGACCTTCGGGT 420


5853R-1.IR_full       421 GCAGGAAAGTCCACTTTGCTAGATGCCCTTTCAGGTTTCAAGACGACCGGAGTGGATGGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5853R-1.in silico     421 GCAGGAAAGTCCACTTTGCTAGATGCCCTTTCAGGTTTCAAGACGACCGGAGTGGATGGC 480


5853R-1.IR_full       481 TCCATCTTGCTCAATGGTCG 500
                          ||||||||||||||||||||
5853R-1.in silico     481 TCCATCTTGCTCAATGGTCG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_176005.1	CG5853-RA (CG5853), mRNA
0.2	1	4	2	3	NM_057679.4	CG5371-RA (RnrL), mRNA
0	0	3	3	3	NM_166303.1	CG30121-RA (CG30121), mRNA
0	0	2	8	6	NM_176162.1	CG33138-RA (CG33138), mRNA
0	0	2	4	5	NM_134774.2	CG17646-RA, transcript variant A (CG17646), mRNA
0	0	2	4	5	NM_164436.1	CG17646-RB, transcript variant B (CG17646), mRNA
0	0	2	3	4	NM_001042891.1	CG31761-RE, transcript variant E (bru-2), mRNA
0	0	2	3	4	NM_205900.1	CG3327-RC, transcript variant C (E23), mRNA
0	0	2	3	4	NM_079909.2	CG3327-RA, transcript variant A (E23), mRNA
0	0	2	3	3	NM_132522.2	CG32662-RA (CG32662), mRNA
0	0	2	3	2	NM_134924.1	CG3327-RB, transcript variant B (E23), mRNA
0	0	1	8	6	NM_205957.1	CG33304-RA (rho-5), mRNA
0	0	1	6	7	NM_132297.4	CG7766-RA, transcript variant A (CG7766), mRNA
0	0	1	6	7	NM_176716.2	CG7766-RB, transcript variant B (CG7766), mRNA
0	0	1	6	3	NM_136810.1	CG9079-RA (CG9079), mRNA
0	0	1	6	2	NM_130493.1	CG13365-RA (CG13365), mRNA
0	0	1	4	3	NM_170332.1	CG5938-RB, transcript variant B (CG5938), mRNA
0	0	1	4	3	NM_170333.1	CG5938-RD, transcript variant D (CG5938), mRNA
0	0	1	4	3	NM_143296.2	CG5938-RC, transcript variant C (CG5938), mRNA
0	0	1	4	3	NM_170331.1	CG5938-RA, transcript variant A (CG5938), mRNA
0	0	1	3	8	NM_140645.1	CG13033-RA (CG13033), mRNA
0	0	1	3	7	NM_167028.1	CG6775-RB, transcript variant B (rg), mRNA
0	0	1	3	7	NM_001042796.1	CG6775-RC, transcript variant C (rg), mRNA
0	0	1	3	7	NM_080023.1	CG6775-RA, transcript variant A (rg), mRNA
0	0	1	3	4	NM_142957.1	CG13605-RA (CG13605), mRNA
0	0	1	2	6	NM_078880.2	CG10443-RA (Lar), mRNA
0	0	1	2	6	NM_169632.1	CG6384-RB, transcript variant B (Cp190), mRNA
0	0	1	2	6	NM_079635.2	CG6384-RA, transcript variant A (Cp190), mRNA
0	0	1	2	4	NM_001015398.1	CG41125-PA (CG41125), mRNA
0	0	1	2	3	NM_141324.1	CG1098-RA (Madm), mRNA

Back