NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

5848R-3

Symbol

cact

Full Name

cactus

CG No

CG5848

Old CG No

CG5848

Synonyms

CACT, CG5848, dip6, n(2)k17003, BG:DS02740.15, cac, fs(2)ltoRN48, cact

Accession No (Link to NCBI)

NM_165152.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Li Z, Wu C, Ding X, Li W, Xue L.
Toll signaling promotes JNK-dependent apoptosis in Drosophila.
Cell Div (2020) 15 7 [ PubMed ID = 32174999 ] [ RRC reference ]

Valanne S, Salminen TS, Järvelä-Stölting M, Vesala L, Rämet M.
Immune-inducible non-coding RNA molecule lincRNA-IBIN connects immunity and metabolism in Drosophila melanogaster.
PLoS Pathog (2019) 15(1) e1007504 [ PubMed ID = 30633769 ] [ RRC reference ]

Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354 [ PubMed ID = 29764959 ] [ RRC reference ]

Wang CH, Huang YC, Chen PY, Cheng YJ, Kao HH, Pi H, Chien CT.
USP5/Leon deubiquitinase confines postsynaptic growth by maintaining ubiquitin homeostasis through Ubiquilin.
Elife (2017) 6 [ PubMed ID = 28489002 ] [ RRC reference ]

Wu C, Chen Y, Wang F, Chen C, Zhang S, Li C, Li W, Wu S, Xue L.
Pelle Modulates dFoxO-Mediated Cell Death in Drosophila.
PLoS Genet (2015) 11(10) e1005589 [ PubMed ID = 26474173 ] [ RRC reference ]

Wu C, Chen C, Dai J, Zhang F, Chen Y, Li W, Pastor-Pareja JC, Xue L.
Toll pathway modulates TNF-induced JNK-dependent cell death in Drosophila.
Open Biol (2015) 5(7) 140171 [ PubMed ID = 26202785 ] [ RRC reference ]

Zhou B, Lindsay SA, Wasserman SA.
Alternative NF-κB Isoforms in the Drosophila Neuromuscular Junction and Brain.
PLoS One (2015) 10(7) e0132793 [ PubMed ID = 26167685 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCAACAAAAGCAGCGGAGGCA

Primer Seq. 3'

aatctagaggtaccTGTTCGCCGCGACTGACCAAA

Predicted Fragment Size

IR fragment full Seq

0001 CCAACAAAAG CAGCGGAGGC AGCAACAAAG GCAACAGCAA CAAGTGACTG CAGCTGCAgC 
0061 GCTGCCAGTG TTGAGCAGCG CGCCCCCTCA AACGCGGCTA ACCCCTCGTC CTCACTAGCC 
0121 ACTAGCGGTA AAATTGGCGG CAAAACACAA GATCAAACGG CCGCCATTAA CAAGCAAAAG 
0181 GAATTTGCCG TGCCAAACGA AACCAGTGAT TCGGGCTTCA TCTCCGGCCC GCAATCCTCG 
0241 CAGATCTTCA GCGAGGAGAT AGTCCCCGAT AGCGAGGAAC AGGATAAGGA TCAGCAGGAG 
0301 TCGGCACCCC AAAAGGAACA GCCCGTTGTC CTCGATTCCG GTATTATCGA CGAAGAAGAG 
0361 GATCAGGAGG AGCAGGAGAA AGAGGAGGAG CACCAGGACA CCACCACTGC CACCGCCGAT 
0421 TCAATGCGAC TCAAACACAG CGCGGACACA GGCATCCCAC AATGGACCGT CGAAAGCCAT 
0481 TTGGTCAGTC GCGGCGAACA

in silico PCR Fragment

0001 CCAACAAAAG CAGCGGAGGC AGCAACAAAG GCAACAGCAA CAAGTGACTG CAGCTGCAGC 
0061 GCTGCCAGTG TTGAGCAGCG CGCCCCCTCA AACGCGGCTA ACCCCTCGTC CTCACTAGCC 
0121 ACTAGCGGTA AAATTGGCGG CAAAACACAA GATCAAACGG CCGCCATTAA CAAGCAAAAG 
0181 GAATTTGCCG TGCCAAACGA AACCAGTGAT TCGGGCTTCA TCTCCGGCCC GCAATCCTCG 
0241 CAGATCTTCA GCGAGGAGAT AGTCCCCGAT AGCGAGGAAC AGGATAAGGA TCAGCAGGAG 
0301 TCGGCACCCC AAAAGGAACA GCCCGTTGTC CTCGATTCCG GTATTATCGA CGAAGAAGAG 
0361 GATCAGGAGG AGCAGGAGAA AGAGGAGGAG CACCAGGACA CCACCACTGC CACCGCCGAT 
0421 TCAATGCGAC TCAAACACAG CGCGGACACA GGCATCCCAC AATGGACCGT CGAAAGCCAT 
0481 TTGGTCAGTC GCGGCGAACA

Assemble Data


5848R-3.IR_full       1   CCAACAAAAGCAGCGGAGGCAGCAACAAAGGCAACAGCAACAAGTGACTGCAGCTGCAGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5848R-3.in silico     1   CCAACAAAAGCAGCGGAGGCAGCAACAAAGGCAACAGCAACAAGTGACTGCAGCTGCAGC 60


5848R-3.IR_full       61  GCTGCCAGTGTTGAGCAGCGCGCCCCCTCAAACGCGGCTAACCCCTCGTCCTCACTAGCC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5848R-3.in silico     61  GCTGCCAGTGTTGAGCAGCGCGCCCCCTCAAACGCGGCTAACCCCTCGTCCTCACTAGCC 120


5848R-3.IR_full       121 ACTAGCGGTAAAATTGGCGGCAAAACACAAGATCAAACGGCCGCCATTAACAAGCAAAAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5848R-3.in silico     121 ACTAGCGGTAAAATTGGCGGCAAAACACAAGATCAAACGGCCGCCATTAACAAGCAAAAG 180


5848R-3.IR_full       181 GAATTTGCCGTGCCAAACGAAACCAGTGATTCGGGCTTCATCTCCGGCCCGCAATCCTCG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5848R-3.in silico     181 GAATTTGCCGTGCCAAACGAAACCAGTGATTCGGGCTTCATCTCCGGCCCGCAATCCTCG 240


5848R-3.IR_full       241 CAGATCTTCAGCGAGGAGATAGTCCCCGATAGCGAGGAACAGGATAAGGATCAGCAGGAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5848R-3.in silico     241 CAGATCTTCAGCGAGGAGATAGTCCCCGATAGCGAGGAACAGGATAAGGATCAGCAGGAG 300


5848R-3.IR_full       301 TCGGCACCCCAAAAGGAACAGCCCGTTGTCCTCGATTCCGGTATTATCGACGAAGAAGAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5848R-3.in silico     301 TCGGCACCCCAAAAGGAACAGCCCGTTGTCCTCGATTCCGGTATTATCGACGAAGAAGAG 360


5848R-3.IR_full       361 GATCAGGAGGAGCAGGAGAAAGAGGAGGAGCACCAGGACACCACCACTGCCACCGCCGAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5848R-3.in silico     361 GATCAGGAGGAGCAGGAGAAAGAGGAGGAGCACCAGGACACCACCACTGCCACCGCCGAT 420


5848R-3.IR_full       421 TCAATGCGACTCAAACACAGCGCGGACACAGGCATCCCACAATGGACCGTCGAAAGCCAT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5848R-3.in silico     421 TCAATGCGACTCAAACACAGCGCGGACACAGGCATCCCACAATGGACCGTCGAAAGCCAT 480


5848R-3.IR_full       481 TTGGTCAGTCGCGGCGAACA 500
                          ||||||||||||||||||||
5848R-3.in silico     481 TTGGTCAGTCGCGGCGAACA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	6	NM_057594.3	CG5848-RB, transcript variant B (cact), mRNA
100	482	0	0	6	NM_057595.3	CG5848-RC, transcript variant C (cact), mRNA
100	482	0	0	4	NM_165152.1	CG5848-RA, transcript variant A (cact), mRNA
100	482	0	0	4	NM_205999.1	CG5848-RD, transcript variant D (cact), mRNA
0.41	2	5	2	5	NM_132166.2	CG1999-RA (CG1999), mRNA
0.41	2	3	3	3	NM_142267.2	CG5916-RA (CG5916), mRNA
0.41	2	2	6	14	NM_169584.1	CG31330-RA (CG31330), mRNA
0.41	2	2	2	3	NM_137231.2	CG8388-RA (CG8388), mRNA
0	0	4	15	93	NM_133114.2	CG32541-RA (CG32541), mRNA
0	0	4	4	9	NM_206714.1	CG5541-RB, transcript variant B (CG5541), mRNA
0	0	4	4	9	NM_206715.1	CG5541-RA, transcript variant A (CG5541), mRNA
0	0	4	4	3	NM_143364.1	CG12413-RA (CG12413), mRNA
0	0	4	3	8	NM_176130.3	CG12052-RJ, transcript variant J (lola), mRNA
0	0	4	3	4	NM_079581.2	CG6538-RA (TfIIFbeta), mRNA
0	0	4	2	11	NM_141277.2	CG1172-RA (CG1172), mRNA
0	0	3	14	71	NM_131975.3	CG32767-RA, transcript variant A (CG32767), mRNA
0	0	3	14	71	NM_206628.1	CG32767-RB, transcript variant B (CG32767), mRNA
0	0	3	7	2	NM_080097.2	CG6699-RA (beta'Cop), mRNA
0	0	3	5	9	NM_132239.1	CG1632-RA (CG1632), mRNA
0	0	3	4	25	NM_206091.1	CG7734-RD, transcript variant D (shn), mRNA
0	0	3	4	25	NM_057375.3	CG7734-RA, transcript variant A (shn), mRNA
0	0	3	4	25	NM_134279.2	CG7734-RB, transcript variant B (shn), mRNA
0	0	3	4	25	NM_057376.2	CG7734-RC, transcript variant C (shn), mRNA
0	0	3	4	13	NM_132068.1	CG15899-RB (Ca-alpha1T), mRNA
0	0	3	3	21	NM_141072.2	CG7177-RA (CG7177), mRNA
0	0	3	3	13	NM_206008.1	CG33316-RB, transcript variant B (CG33316), mRNA
0	0	3	3	13	NM_206007.1	CG33316-RA, transcript variant A (CG33316), mRNA
0	0	3	3	4	NM_206773.1	CG32556-RB, transcript variant B (CG32556), mRNA
0	0	3	2	21	NM_132959.2	CG8949-RA (CG8949), mRNA
0	0	3	2	2	NM_170325.1	CG31064-RA, transcript variant A (CG31064), mRNA

Back