NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 5828R-3

Symbol CG5828 Full Name CG5828

CG No CG5828 Old CG No CG5828

Synonyms CG5828

Accession No (Link to NCBI) NM_135063.1

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGCTGTCTATCAGCCGGACAC

Primer Seq. 3'

aatctagaggtaccCCAGGGCTGAATGAAGTCCGA

Predicted Fragment Size

IR fragment full Seq

0001 GGNTNTCTAT CAGCCGGACA CCCTGGACCT GAACACGGAT GGAAAGGCGG CGGATTACTG 
0061 GTTCAACTGC TTCCGCGACA TGGTAGCCAA GTTCGCGAAG GTGGCGGCCA AGTCACAGGA 
0121 GGAGGATCAC ACGGCCACCC AGCGGGCGGA GCANTTCCAG GCAGCGTATT TGCAGCGGCT 
0181 GGAGGAGCAC CAGCAGAATG TGCCAGTCAA CAAGGGCAAG GTGATTCTAG GCACCAGTGA 
0241 GCTTCTCAAG CTAAACGAAA CCCTGTTGCG CCGCTACGGN ATTTACAGAT CCCTGGCTGA 
0301 GACAAAAGAG GCTGGAAAAT GCATCGGCTG TTGCCCGCCT GAAGCAGCGT CTGCAGGAAT 
0361 TGNGATGCCC TGGCGGNATG AGGACACAAG GTGGACGGAG CTGGTACGCG GTGTCCTCGC 
0421 CGGCAACATG TTTGACTGGG GCGCACAGGC CATATCTAAC ATACTGGAGC AGGACAGCAA 
0481 TTTCGGACTT CATTCAGCCC TGG

in silico PCR Fragment

0001 GGCTGTCTAT CAGCCGGACA CCCTGGACCT GAACACGGAT GGAAAGGCGG CGGATTACTG 
0061 GTTCAACTGC TTCCGCGACA TGGTGGCCAA GTTCGCGAAG GTGGCGGCCA AGTCACAGGA 
0121 GGAGGATCAC ACGGCCACCC AGCGGGCGGA GCAGTTCCAG GCGGCGTATC TGCAGCGGCT 
0181 GGAGGAGCAC CAGCGTAATG TGCCAGTCAA CAAGGGCAAG GTGATTCTAG GCACCAGTGA 
0241 GCTTCTCAAG CTAAACGAAA CCCTGTTGCG CCGCTACGG- ATTTACAGAT CCCTGGCTGA 
0301 GACAAAAGAG GCTGGAAAAT GCATCGGCTG TTGCCCGCCT GAAGCAGCGT CTGCAGGAAT 
0361 TG-GATGCCC TGGCGG-ATG AGGACACAAG GTGGACGGAG CTGGTACGCG GTGTCCTCGC 
0421 CGGCAACATG TTTGACTGGG GCGCACAGGC CATATCTAAC ATACTGGAGC AGGACAGCAA 
0481 TTTCGGACTT CATTCAGCCC TGG

Assemble Data


5828R-3.IR_full       1   GGNTNTCTATCAGCCGGACACCCTGGACCTGAACACGGATGGAAAGGCGGCGGATTACTG 60
                          || | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
5828R-3.in silico     1   GGCTGTCTATCAGCCGGACACCCTGGACCTGAACACGGATGGAAAGGCGGCGGATTACTG 60


5828R-3.IR_full       61  GTTCAACTGCTTCCGCGACATGGTAGCCAAGTTCGCGAAGGTGGCGGCCAAGTCACAGGA 120
                          |||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
5828R-3.in silico     61  GTTCAACTGCTTCCGCGACATGGTGGCCAAGTTCGCGAAGGTGGCGGCCAAGTCACAGGA 120


5828R-3.IR_full       121 GGAGGATCACACGGCCACCCAGCGGGCGGAGCANTTCCAGGCAGCGTATTTGCAGCGGCT 180
                          ||||||||||||||||||||||||||||||||| |||||||| |||||| ||||||||||
5828R-3.in silico     121 GGAGGATCACACGGCCACCCAGCGGGCGGAGCAGTTCCAGGCGGCGTATCTGCAGCGGCT 180


5828R-3.IR_full       181 GGAGGAGCACCAGCAGAATGTGCCAGTCAACAAGGGCAAGGTGATTCTAGGCACCAGTGA 240
                          ||||||||||||||  ||||||||||||||||||||||||||||||||||||||||||||
5828R-3.in silico     181 GGAGGAGCACCAGCGTAATGTGCCAGTCAACAAGGGCAAGGTGATTCTAGGCACCAGTGA 240


5828R-3.IR_full       241 GCTTCTCAAGCTAAACGAAACCCTGTTGCGCCGCTACGGNATTTACAGATCCCTGGCTGA 300
                          ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
5828R-3.in silico     241 GCTTCTCAAGCTAAACGAAACCCTGTTGCGCCGCTACGG-ATTTACAGATCCCTGGCTGA 300


5828R-3.IR_full       301 GACAAAAGAGGCTGGAAAATGCATCGGCTGTTGCCCGCCTGAAGCAGCGTCTGCAGGAAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5828R-3.in silico     301 GACAAAAGAGGCTGGAAAATGCATCGGCTGTTGCCCGCCTGAAGCAGCGTCTGCAGGAAT 360


5828R-3.IR_full       361 TGNGATGCCCTGGCGGNATGAGGACACAAGGTGGACGGAGCTGGTACGCGGTGTCCTCGC 420
                          || ||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
5828R-3.in silico     361 TG-GATGCCCTGGCGG-ATGAGGACACAAGGTGGACGGAGCTGGTACGCGGTGTCCTCGC 420


5828R-3.IR_full       421 CGGCAACATGTTTGACTGGGGCGCACAGGCCATATCTAACATACTGGAGCAGGACAGCAA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5828R-3.in silico     421 CGGCAACATGTTTGACTGGGGCGCACAGGCCATATCTAACATACTGGAGCAGGACAGCAA 480


5828R-3.IR_full       481 TTTCGGACTTCATTCAGCCCTGG 503
                          |||||||||||||||||||||||
5828R-3.in silico     481 TTTCGGACTTCATTCAGCCCTGG 503

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_135063.1	CG5828-RA (CG5828), mRNA
0	0	2	4	3	NM_132032.1	CG15769-RA (CG15769), mRNA
0	0	2	3	3	NM_138132.1	CG9083-RA (CG9083), mRNA
0	0	2	3	3	NM_206004.1	CG10719-RC, transcript variant C (brat), mRNA
0	0	2	3	3	NM_134302.1	CG10719-RB, transcript variant B (brat), mRNA
0	0	2	3	3	NM_057597.2	CG10719-RA, transcript variant A (brat), mRNA
0	0	2	3	2	NM_205952.1	CG4128-RD, transcript variant D (nAcRalpha-30D), mRNA
0	0	2	3	2	NM_205953.1	CG4128-RE, transcript variant E (nAcRalpha-30D), mRNA
0	0	2	3	2	NM_164874.2	CG4128-RA, transcript variant A (nAcRalpha-30D), mRNA
0	0	2	3	2	NM_205951.1	CG4128-RB, transcript variant B (nAcRalpha-30D), mRNA
0	0	2	3	2	NM_135472.4	CG4128-RC, transcript variant C (nAcRalpha-30D), mRNA
0	0	2	2	2	NM_132771.1	CG15027-RA (CG15027), mRNA
0	0	1	6	8	NM_176694.1	CG3126-RB, transcript variant B (C3G), mRNA
0	0	1	6	8	NM_132122.2	CG3126-RA, transcript variant A (C3G), mRNA
0	0	1	5	3	NM_142945.2	CG13604-RA (CG13604), mRNA
0	0	1	4	4	NM_137987.2	CG5539-RA (CG5539), mRNA
0	0	1	4	3	NM_137130.2	CG10131-RA (CG10131), mRNA
0	0	1	4	3	NM_167259.1	CG1615-RA, transcript variant A (Ork1), mRNA
0	0	1	4	3	NM_078557.4	CG1615-RB, transcript variant B (Ork1), mRNA
0	0	1	3	6	NM_130610.1	CG3600-RA (CG3600), mRNA
0	0	1	3	3	NM_079411.2	CG7347-RA (mus304), mRNA
0	0	1	2	10	NM_132074.1	CG3585-RA (CG3585), mRNA
0	0	1	2	7	NM_168841.1	CG32226-RA (CG32226), mRNA
0	0	1	2	7	NM_168794.2	CG32206-RB, transcript variant B (CG32206), mRNA
0	0	1	2	7	NM_176360.1	CG32206-RC, transcript variant C (CG32206), mRNA
0	0	1	2	6	NM_057841.3	CG5102-RA (da), mRNA
0	0	1	2	3	NM_001031867.1	CG33950-RF, transcript variant F (trol), mRNA
0	0	1	2	3	NM_144357.2	CG13475-RA (HGTX), mRNA
0	0	1	2	2	NM_001031862.1	CG33950-RC, transcript variant C (trol), mRNA
0	0	1	2	2	NM_001031865.1	CG33950-RB, transcript variant B (trol), mRNA

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