NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 5807R-1

Symbol CG5807 Full Name CG5807

CG No CG5807 Old CG No CG5807

Synonyms BcDNA:GH12663, anon-WO0118547.355, CG5807

Accession No (Link to NCBI) NM_143033.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGACGAAGAGGAGGAGGAG

Primer Seq. 3'

aatctagaggtaccAAGGTTTCGTAGACTCGCGGC

Predicted Fragment Size

IR fragment full Seq

0001 ATGGAACGAA GAGGAGGAGG AGGAGGTTAC GGTATCTAAA GCTGCAATTG TTCCACAATA 
0061 CAGTACGCGA GCACATTATA TTCTTGCTAC TGATCATCTT GCTGTACTCC AGCTCCTATG 
0121 TGGTCGTCTC ACGTTTTAGG CGGCGCGACC GTGACGATCT CTATTCGAAT GACGAGGACG 
0181 AGGTGCTGGT CTATCGCATA AGCTTTTGGC TGTGCACATT CACACTGGCC GTTGCCGAAG 
0241 GGGCTGCCAT GCTGCTGCCC GTTTCCATTG CCAGCAATGA GGTGCTACTC CTCTATCCCA 
0301 ACAGTTACTA TGTGAAATGG CTAAACAGTT CACTCATACA GGGCTTGTGG AACCATGTAT 
0361 TTCTGTTCTC CAATCTGTCA CTGTTCATCT TCCTGCCATT TGTCTATTTG TTCTCGGAAT 
0421 CCACGGGATT CGTCGGCAAC AAGAAGGGCA TCCTGCCGCG AGTCTACGAA ACCTT

in silico PCR Fragment

0001 ATGG-ACGAA GAGGAGGAGG AGGAGGTTAC GG-ATCTAAA GCTGCAATTG TTCCACAATA 
0061 CAGTACGCGA GCACATTATA TTCTTGCTAC TGATAATCTT GCTGTACTCC AGCTCCTATG 
0121 TGGTCGTCTC ACGTTTTAGG CGGCGCGACC GTGACGATCT CTATTCGAAT GACGAGGACG 
0181 AGGTGCTGGT CTATCGCATA AGCTTTTGGC TGTGCACATT CACACTGGCC GTTGCCGAAG 
0241 GGGCTGCCAT GCTGCTGCCC GTTTCCATTG CCAGCAATGA GGTGCTACTC CTCTATCCCA 
0301 ACAGTTACTA TGTGAAATGG CTAAACAGTT CACTCATACA GGGCTTGTGG AACCATGTAT 
0361 TTCTGTTCTC CAATCTGTCA CTGTTCATCT TCCTGCCATT TGTCTATTTG TTCTCGGAAT 
0421 CCACGGGATT CGTCGGCAAC AAGAAGGGCA TCCTGCCGCG AGTCTACGAA ACCTT

Assemble Data


5807R-1.IR_full       1   ATGGAACGAAGAGGAGGAGGAGGAGGTTACGGTATCTAAAGCTGCAATTGTTCCACAATA 60
                          |||| ||||||||||||||||||||||||||| |||||||||||||||||||||||||||
5807R-1.in silico     1   ATGG-ACGAAGAGGAGGAGGAGGAGGTTACGG-ATCTAAAGCTGCAATTGTTCCACAATA 60


5807R-1.IR_full       61  CAGTACGCGAGCACATTATATTCTTGCTACTGATCATCTTGCTGTACTCCAGCTCCTATG 120
                          |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
5807R-1.in silico     61  CAGTACGCGAGCACATTATATTCTTGCTACTGATAATCTTGCTGTACTCCAGCTCCTATG 120


5807R-1.IR_full       121 TGGTCGTCTCACGTTTTAGGCGGCGCGACCGTGACGATCTCTATTCGAATGACGAGGACG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5807R-1.in silico     121 TGGTCGTCTCACGTTTTAGGCGGCGCGACCGTGACGATCTCTATTCGAATGACGAGGACG 180


5807R-1.IR_full       181 AGGTGCTGGTCTATCGCATAAGCTTTTGGCTGTGCACATTCACACTGGCCGTTGCCGAAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5807R-1.in silico     181 AGGTGCTGGTCTATCGCATAAGCTTTTGGCTGTGCACATTCACACTGGCCGTTGCCGAAG 240


5807R-1.IR_full       241 GGGCTGCCATGCTGCTGCCCGTTTCCATTGCCAGCAATGAGGTGCTACTCCTCTATCCCA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5807R-1.in silico     241 GGGCTGCCATGCTGCTGCCCGTTTCCATTGCCAGCAATGAGGTGCTACTCCTCTATCCCA 300


5807R-1.IR_full       301 ACAGTTACTATGTGAAATGGCTAAACAGTTCACTCATACAGGGCTTGTGGAACCATGTAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5807R-1.in silico     301 ACAGTTACTATGTGAAATGGCTAAACAGTTCACTCATACAGGGCTTGTGGAACCATGTAT 360


5807R-1.IR_full       361 TTCTGTTCTCCAATCTGTCACTGTTCATCTTCCTGCCATTTGTCTATTTGTTCTCGGAAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5807R-1.in silico     361 TTCTGTTCTCCAATCTGTCACTGTTCATCTTCCTGCCATTTGTCTATTTGTTCTCGGAAT 420


5807R-1.IR_full       421 CCACGGGATTCGTCGGCAACAAGAAGGGCATCCTGCCGCGAGTCTACGAAACCTT 475
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||
5807R-1.in silico     421 CCACGGGATTCGTCGGCAACAAGAAGGGCATCCTGCCGCGAGTCTACGAAACCTT 475

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	455	0	2	4	NM_143033.2	CG5807-RA (CG5807), mRNA
0.87	4	4	16	65	NM_132413.1	CG15295-RA (CG15295), mRNA
0.87	4	2	10	27	NM_131978.1	CG15465-RA (CG15465), mRNA
0.43	2	5	5	8	NM_132375.1	CG2972-RA (CG2972), mRNA
0.43	2	3	4	9	NM_140816.1	CG6841-RA (CG6841), mRNA
0.21	1	8	15	21	NM_142121.1	CG7886-RA (CG7886), mRNA
0.21	1	7	9	11	NM_132709.1	CG12480-RA (CG12480), mRNA
0.21	1	6	12	16	NM_142100.2	CG8524-RA, transcript variant A (NK7.1), mRNA
0.21	1	6	12	16	NM_169580.1	CG8524-RB, transcript variant B (NK7.1), mRNA
0.21	1	5	7	16	NM_139971.1	CG6915-RA (CG6915), mRNA
0.21	1	3	2	7	NM_057407.2	CG8409-RA, transcript variant A (Su(var)205), mRNA
0.21	1	3	2	7	NM_164799.1	CG8409-RB, transcript variant B (Su(var)205), mRNA
0.21	1	2	5	8	NM_079847.3	CG2184-RA, transcript variant A (Mlc2), mRNA
0.21	1	2	5	8	NM_170474.2	CG2184-RB, transcript variant B (Mlc2), mRNA
0	0	11	45	99	NM_001014738.1	CG7107-RF, transcript variant F (up), mRNA
0	0	11	45	99	NM_167375.1	CG7107-RB, transcript variant B (up), mRNA
0	0	11	45	99	NM_167376.1	CG7107-RD, transcript variant D (up), mRNA
0	0	11	45	99	NM_080349.2	CG7107-RA, transcript variant A (up), mRNA
0	0	11	45	99	NM_001014737.1	CG7107-RG, transcript variant G (up), mRNA
0	0	11	45	97	NM_001014739.1	CG7107-RE, transcript variant E (up), mRNA
0	0	10	24	27	NM_167309.1	CG32663-RA (CG32663), mRNA
0	0	8	27	25	NM_140288.2	CG6801-RA (l(3)j2D3), mRNA
0	0	8	13	18	NM_143338.2	CG12259-RA (CG12259), mRNA
0	0	8	12	33	NM_140897.2	CG8103-RA, transcript variant A (Mi-2), mRNA
0	0	8	12	33	NM_001014591.1	CG8103-RB, transcript variant B (Mi-2), mRNA
0	0	8	9	13	NM_137247.1	CG8434-RA (lbk), mRNA
0	0	7	43	47	NM_167524.2	CG9819-RB, transcript variant B (CanA-14F), mRNA
0	0	7	43	47	NM_167523.2	CG9819-RA, transcript variant A (CanA-14F), mRNA
0	0	7	17	37	NM_133104.2	CG7282-RA (CG7282), mRNA
0	0	7	15	14	NM_164653.1	CG31646-RA (CG31646), mRNA

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