NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 5789R-1

Symbol CG5789 Full Name CG5789

CG No CG5789 Old CG No CG5789

Synonyms Dme_CG5789, CG5789

Accession No (Link to NCBI) NM_143012.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal

Map Viewer

Reference
[Please submit your publication] Kim K, Lane EA, Saftien A, Wang H, Xu Y, Wirtz-Peitz F, Perrimon N.
Drosophila as a model for studying cystic fibrosis pathophysiology of the gastrointestinal system.
Proc Natl Acad Sci U S A (2020) 117(19) 10357-10367 [ PubMed ID = 32345720 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGGAGCCGATTCAGCCTCCAG

Primer Seq. 3'

aatctagaggtaccCTCCGCAATTCTCATCCGCAG

Predicted Fragment Size

IR fragment full Seq

0001 TGGAGCCGAT TCAGCCTCCA GTAGAGGAAG TGGATCACAA TGCAACCGCT GTGCGAAGCA 
0061 TCNTTGGATC AGACCGTCGA GCACATGTGG AACGATATGT ACAGTCTGGG CGCCGTCCTG 
0121 GTTCTTTCGA CGCTGTTCTG CTGCTTCCTG TTGCACCACG TCGATCTCCG ACAGCGCCTA 
0181 ATGGGTGCAC GGATGCGAAT AGCCTGCTGC TCGCTGATCT ACCGGAAAAC TCTACGGCTC 
0241 TCGATGAAAA CGGCTGGTCA GACGCCAGCT GGTTACCTAA TCAACCTGCT CTCGAATGAC 
0301 GTGAATCGTT TGGACTATGG CTTCATATTC ATGCACTGGA TCTGGATTAT GCCATTGCAG 
0361 GCGATGCTCA CATGCTACCT GATTTGGCTG AACATTGGCA TCCCAGCAGT TGTGGGTGTA 
0421 GTTGGACTGC TGCTAAAAAC GGTGCCCGTG CAGACGGCCT TGAGCAAGGT AACCTCTGTG 
0481 CTGCGGATGA GAATTGCGGA G

in silico PCR Fragment

0001 TGGAGCCGAT TCAGCCTCCA GTAGAGGAAG TGGATCACAA TGCAACCGCT GTGCGAAGCA 
0061 TC-TTGGATC AGACCGTCGA GCACATGTGG AACGATATGA ACAGTCTGGG CGCCGTCCTG 
0121 GTTCTTTCGA CGCTGTTCTG CTGCTTCCTG TTGCACCACG TCGATCTCCG ACAGCGCCTA 
0181 ATGGGTGCAC GGATGCGAAT AGCCTGCTGC TCGCTGATCT ACCGGAAAAC TCTACGGCTC 
0241 TCGATGAAAA CGGCTGGTCA GACGCCAGCT GGTTACCTAA TCAACCTTCT TTCGAATGAT 
0301 GTGAATCGTT TGGACTATGG CTTCATATTC ATGCACTGGA TCTGGATTAT GCCATTGCAG 
0361 GCGATGCTCA CGTGCTACCT GATTTGGCTG AACATTGGCA TCCCAGCAAT TGTGGGTGTA 
0421 GTTGGACTGC TGCTAAAAAC GGTGCCCGTG CAGACGGCCT TGAGCAAGGT AACCTCTGTG 
0481 CTGCGGATGA GAATTGCGGA G

Assemble Data


5789R-1.IR_full       1   TGGAGCCGATTCAGCCTCCAGTAGAGGAAGTGGATCACAATGCAACCGCTGTGCGAAGCA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5789R-1.in silico     1   TGGAGCCGATTCAGCCTCCAGTAGAGGAAGTGGATCACAATGCAACCGCTGTGCGAAGCA 60


5789R-1.IR_full       61  TCNTTGGATCAGACCGTCGAGCACATGTGGAACGATATGTACAGTCTGGGCGCCGTCCTG 120
                          || |||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
5789R-1.in silico     61  TC-TTGGATCAGACCGTCGAGCACATGTGGAACGATATGAACAGTCTGGGCGCCGTCCTG 120


5789R-1.IR_full       121 GTTCTTTCGACGCTGTTCTGCTGCTTCCTGTTGCACCACGTCGATCTCCGACAGCGCCTA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5789R-1.in silico     121 GTTCTTTCGACGCTGTTCTGCTGCTTCCTGTTGCACCACGTCGATCTCCGACAGCGCCTA 180


5789R-1.IR_full       181 ATGGGTGCACGGATGCGAATAGCCTGCTGCTCGCTGATCTACCGGAAAACTCTACGGCTC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5789R-1.in silico     181 ATGGGTGCACGGATGCGAATAGCCTGCTGCTCGCTGATCTACCGGAAAACTCTACGGCTC 240


5789R-1.IR_full       241 TCGATGAAAACGGCTGGTCAGACGCCAGCTGGTTACCTAATCAACCTGCTCTCGAATGAC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||| || |||||||| 
5789R-1.in silico     241 TCGATGAAAACGGCTGGTCAGACGCCAGCTGGTTACCTAATCAACCTTCTTTCGAATGAT 300


5789R-1.IR_full       301 GTGAATCGTTTGGACTATGGCTTCATATTCATGCACTGGATCTGGATTATGCCATTGCAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5789R-1.in silico     301 GTGAATCGTTTGGACTATGGCTTCATATTCATGCACTGGATCTGGATTATGCCATTGCAG 360


5789R-1.IR_full       361 GCGATGCTCACATGCTACCTGATTTGGCTGAACATTGGCATCCCAGCAGTTGTGGGTGTA 420
                          ||||||||||| |||||||||||||||||||||||||||||||||||| |||||||||||
5789R-1.in silico     361 GCGATGCTCACGTGCTACCTGATTTGGCTGAACATTGGCATCCCAGCAATTGTGGGTGTA 420


5789R-1.IR_full       421 GTTGGACTGCTGCTAAAAACGGTGCCCGTGCAGACGGCCTTGAGCAAGGTAACCTCTGTG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5789R-1.in silico     421 GTTGGACTGCTGCTAAAAACGGTGCCCGTGCAGACGGCCTTGAGCAAGGTAACCTCTGTG 480


5789R-1.IR_full       481 CTGCGGATGAGAATTGCGGAG 501
                          |||||||||||||||||||||
5789R-1.in silico     481 CTGCGGATGAGAATTGCGGAG 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_143012.1	CG5789-RA (CG5789), mRNA
0	0	4	2	2	NM_143318.2	CG31057-RA (tau), mRNA
0	0	1	7	9	NM_166876.1	CG14622-RB, transcript variant B (DAAM), mRNA
0	0	1	7	9	NM_166875.1	CG14622-RC, transcript variant C (DAAM), mRNA
0	0	1	7	7	NM_130544.3	CG14622-RA, transcript variant A (DAAM), mRNA
0	0	1	4	4	NM_133149.2	CG8051-RA (CG8051), mRNA
0	0	1	3	2	NM_168353.1	CG32048-RB, transcript variant B (CG32048), mRNA
0	0	1	3	2	NM_168352.1	CG32048-RA, transcript variant A (CG32048), mRNA
0	0	1	2	5	NM_136955.2	CG8815-RA, transcript variant A (Sin3A), mRNA
0	0	1	2	5	NM_165916.1	CG8815-RC, transcript variant C (Sin3A), mRNA
0	0	1	2	5	NM_165915.1	CG8815-RB, transcript variant B (Sin3A), mRNA
0	0	1	2	4	NM_164653.1	CG31646-RA (CG31646), mRNA
0	0	1	2	4	NM_139659.1	CG11357-RA (CG11357), mRNA
0	0	1	2	2	NM_136935.1	CG8834-RA (CG8834), mRNA
0	0	0	5	4	NM_168048.1	CG32261-RA (Gr64a), mRNA
0	0	0	5	2	NM_134595.2	CG1724-RA (CG1724), mRNA
0	0	0	4	5	NM_139612.1	CG1308-RA (CG1308), mRNA
0	0	0	4	4	NM_206253.1	CG12002-RE, transcript variant E (Pxn), mRNA
0	0	0	4	4	NM_206254.1	CG12002-RD, transcript variant D (Pxn), mRNA
0	0	0	4	4	NM_206255.1	CG12002-RC, transcript variant C (Pxn), mRNA
0	0	0	4	4	NM_079167.3	CG12002-RA, transcript variant A (Pxn), mRNA
0	0	0	4	3	NM_141996.2	CG12360-RA, transcript variant A (CG12360), mRNA
0	0	0	4	3	NM_169223.2	CG31369-RA (PQBP-1), mRNA
0	0	0	3	7	NM_079901.2	CG10811-RA (eIF-4G), mRNA
0	0	0	3	5	NM_132576.1	CG15730-RA (CG15730), mRNA
0	0	0	3	5	NM_176445.1	CG33208-RC, transcript variant C (MICAL), mRNA
0	0	0	3	5	NM_176449.1	CG33208-RH, transcript variant H (MICAL), mRNA
0	0	0	3	5	NM_176448.1	CG33208-RG, transcript variant G (MICAL), mRNA
0	0	0	3	5	NM_176446.1	CG33208-RD, transcript variant D (MICAL), mRNA
0	0	0	3	5	NM_176447.1	CG33208-RF, transcript variant F (MICAL), mRNA

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