NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 5737R-4

Symbol dmrt93B Full Name doublesex-Mab related 93B

CG No CG5737 Old CG No CG5737

Synonyms unnamed, CG5737, dmrt93B

Accession No (Link to NCBI) NM_079704.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal

Map Viewer

Reference
[Please submit your publication] Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Molnar C, Casado M, López-Varea A, Cruz C, de Celis JF.
Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing.
Genetics (2012) 192(2) 741-52 [ PubMed ID = 22798488 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCTAATCCCAAGGCCACCAAAA

Primer Seq. 3'

aatctagaggtaccGAGCTGGGCAAGCATGTCAAT

Predicted Fragment Size

IR fragment full Seq

0001 CTAATCCCAA GGCCACCAAA ATAGTTGAAC CCACAACGGC CAAGGTCACC AATCGGGTGC 
0061 CCAAGTGCGC CCGCTGCCGG AACCATGGGA TCATTTCAGA GCTGCGGGGT CACAAGAAGC 
0121 TCTGCACCTA CAAGAACTGC AAGTGCGCCA AGTGTGTCCT GATCTTTGAG AGGCAGCGGA 
0181 TCATGGCCGC TCAGGTTGCC CTGAAGCGCC AGCAGGCGGT GGAGGATGCG ATTGCCATGC 
0241 GGCTGGTGGC CAACAAGACG GGTCGTTCCA TCGATGCACT GCCGCCAGGC AACATCTTCG 
0301 GTCTGACCGT CACGCAGCCT AGTTCGCCGC GTGCCAAGCG GGAGGATGAT CAGGTGGAGA 
0361 AGACCATTTC GGAGGTGGAG CAGCAGCATT TGAAGCAGAG GGAGGAGGAG TACAGCGAAT 
0421 CGCCGGCGGT GGCGCAGGAT CTCAGTGCTC CCCGAAGAGA TAACGTCTCC CAAACGGCCA 
0481 TTGACATGCT TGCCCAGCTC

in silico PCR Fragment

0001 CTAATCCCAA GGCCACCAAA ATAGTTGAAC CCACACCGGC CAAGGTCACC AATCGGGTGC 
0061 CCAAGTGCGC CCGCTGCCGG AACCATGGGA TCATTTCAGA GCTGCGGGGT CACAAGAAGC 
0121 TCTGCACCTA CAAGAACTGC AAGTGCGCCA AGTGTGTCCT GATCTTTGAG AGGCAGCGGA 
0181 TCATGGCCGC TCAGGTTGCC CTGAAGCGCC AGCAGGCGGT GGAGGATGCG ATTGCCATGC 
0241 GGCTGGTGGC CAACAAGACG GGTCGTTCCA TCGATGCACT GCCGCCAGGC AACATCTTCG 
0301 GTCTGACCGT CACGCAGCCT AGTTCGCCGC GTGCCAAGCG GGAGGATGAT CAGGTGGAGA 
0361 AGACCATTTC GGAGGTGGAG CAGCAGCATT TGAAGCAGAG GGAGGAGGAG TACAGCGAAT 
0421 CGCCGGCGGT GGCGCAGGAT CTCAGTGCTC CCCGAAGAGA TAACGTCTCC CAAACGGCCA 
0481 TTGACATGCT TGCCCAGCTC

Assemble Data


5737R-4.IR_full       1   CTAATCCCAAGGCCACCAAAATAGTTGAACCCACAACGGCCAAGGTCACCAATCGGGTGC 60
                          ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
5737R-4.in silico     1   CTAATCCCAAGGCCACCAAAATAGTTGAACCCACACCGGCCAAGGTCACCAATCGGGTGC 60


5737R-4.IR_full       61  CCAAGTGCGCCCGCTGCCGGAACCATGGGATCATTTCAGAGCTGCGGGGTCACAAGAAGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5737R-4.in silico     61  CCAAGTGCGCCCGCTGCCGGAACCATGGGATCATTTCAGAGCTGCGGGGTCACAAGAAGC 120


5737R-4.IR_full       121 TCTGCACCTACAAGAACTGCAAGTGCGCCAAGTGTGTCCTGATCTTTGAGAGGCAGCGGA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5737R-4.in silico     121 TCTGCACCTACAAGAACTGCAAGTGCGCCAAGTGTGTCCTGATCTTTGAGAGGCAGCGGA 180


5737R-4.IR_full       181 TCATGGCCGCTCAGGTTGCCCTGAAGCGCCAGCAGGCGGTGGAGGATGCGATTGCCATGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5737R-4.in silico     181 TCATGGCCGCTCAGGTTGCCCTGAAGCGCCAGCAGGCGGTGGAGGATGCGATTGCCATGC 240


5737R-4.IR_full       241 GGCTGGTGGCCAACAAGACGGGTCGTTCCATCGATGCACTGCCGCCAGGCAACATCTTCG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5737R-4.in silico     241 GGCTGGTGGCCAACAAGACGGGTCGTTCCATCGATGCACTGCCGCCAGGCAACATCTTCG 300


5737R-4.IR_full       301 GTCTGACCGTCACGCAGCCTAGTTCGCCGCGTGCCAAGCGGGAGGATGATCAGGTGGAGA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5737R-4.in silico     301 GTCTGACCGTCACGCAGCCTAGTTCGCCGCGTGCCAAGCGGGAGGATGATCAGGTGGAGA 360


5737R-4.IR_full       361 AGACCATTTCGGAGGTGGAGCAGCAGCATTTGAAGCAGAGGGAGGAGGAGTACAGCGAAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5737R-4.in silico     361 AGACCATTTCGGAGGTGGAGCAGCAGCATTTGAAGCAGAGGGAGGAGGAGTACAGCGAAT 420


5737R-4.IR_full       421 CGCCGGCGGTGGCGCAGGATCTCAGTGCTCCCCGAAGAGATAACGTCTCCCAAACGGCCA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5737R-4.in silico     421 CGCCGGCGGTGGCGCAGGATCTCAGTGCTCCCCGAAGAGATAACGTCTCCCAAACGGCCA 480


5737R-4.IR_full       481 TTGACATGCTTGCCCAGCTC 500
                          ||||||||||||||||||||
5737R-4.in silico     481 TTGACATGCTTGCCCAGCTC 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_079704.1	CG5737-RA (dmrt93B), mRNA
0.2	1	9	8	8	NM_079825.1	CG15504-RA (dmrt99B), mRNA
0.2	1	2	3	4	NM_140521.1	CG6498-RA (CG6498), mRNA
0	0	5	2	8	NM_165853.1	CG13188-RA, transcript variant A (CG13188), mRNA
0	0	5	2	8	NM_136870.1	CG13188-RB, transcript variant B (CG13188), mRNA
0	0	3	6	13	NM_057502.2	CG1322-RB, transcript variant B (zfh1), mRNA
0	0	3	6	9	NM_170523.2	CG1322-RA, transcript variant A (zfh1), mRNA
0	0	3	4	5	NM_080047.2	CG8491-RA (kto), mRNA
0	0	3	3	4	NM_143818.1	CG10240-RA (Cyp6a22), mRNA
0	0	3	3	2	NM_139600.3	CG14995-RB, transcript variant B (CG14995), mRNA
0	0	3	3	2	NM_168059.2	CG14995-RA, transcript variant A (CG14995), mRNA
0	0	3	3	2	NM_168058.2	CG14995-RC, transcript variant C (CG14995), mRNA
0	0	3	3	2	NM_168057.2	CG14995-RD, transcript variant D (CG14995), mRNA
0	0	3	2	3	NM_143019.2	CG6695-RA, transcript variant A (CG6695), mRNA
0	0	3	2	3	NM_206565.1	CG6695-RB, transcript variant B (CG6695), mRNA
0	0	3	2	2	NM_135804.2	CG7099-RA (CG7099), mRNA
0	0	2	4	3	NM_057824.3	CG6601-RA (Rab6), mRNA
0	0	2	4	3	NM_001015160.1	CG12449-PC.3 (CG12449), mRNA
0	0	2	4	3	NM_001015157.1	CG12449-PA.3 (CG12449), mRNA
0	0	2	4	3	NM_001015162.1	CG12449-PF.3 (CG12449), mRNA
0	0	2	4	2	NM_137537.2	CG15099-RA (CG15099), mRNA
0	0	2	3	10	NM_170522.2	CG1322-RC, transcript variant C (zfh1), mRNA
0	0	2	3	2	NM_143234.1	CG14242-RA (CG14242), mRNA
0	0	2	2	7	NM_165520.1	CG11166-RE, transcript variant E (CG11166), mRNA
0	0	2	2	7	NM_206049.1	CG11166-RF, transcript variant F (CG11166), mRNA
0	0	2	2	7	NM_165518.1	CG11166-RA, transcript variant A (CG11166), mRNA
0	0	2	2	7	NM_165521.1	CG11166-RB, transcript variant B (CG11166), mRNA
0	0	2	2	7	NM_206048.1	CG11166-RG, transcript variant G (CG11166), mRNA
0	0	2	2	7	NM_165519.1	CG11166-RC, transcript variant C (CG11166), mRNA
0	0	2	2	7	NM_136429.2	CG11166-RD, transcript variant D (CG11166), mRNA

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