| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5685R-2 |
| Symbol |
Calx |
Full Name |
Na/Ca-exchange protein |
| CG No |
CG5685 |
Old CG No |
CG5685 |
| Synonyms |
CG5685, NCX, CALX1.1, Ncx, Dmel/Ncx, 9C5, unnamed, anon-93ABa, Calx, calX, calx, CalX |
| Accession No (Link to NCBI) |
|
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTTGTGTATGCCACCGCCCAG |
| Primer Seq. 3' |
aatctagaggtaccAGATTCGCCACCGTTTCGTTC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
|
| in silico PCR Fragment |
|
| Assemble Data |
5685R-2.IR full 1 TTTGTGTATGCCACCGCCCAGTCGCTGCTCAAAGTCCAGGAGACAGAAGCGCGGCAGGCG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5685R-2.in silico 1 TTTGTGTATGCCACCGCCCAGTCGCTGCTCAAAGTCCAGGAGACAGAAGCGCGGCAGGCG 60
5685R-2.IR full 61 TACCTTAATGTTACCTCCTCCAGCAGTAGTAATCTCAGCCAGGACGATGGCCACTTCCTC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5685R-2.in silico 61 TACCTTAATGTTACCTCCTCCAGCAGTAGTAATCTCAGCCAGGACGATGGCCACTTCCTC 120
5685R-2.IR full 121 AGCCGGCGACTAAGGCANGTCAGCCATGGCGAGGAGGGCGACGAGGGCGCGCCGTCCCAA 180
||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
5685R-2.in silico 121 AGCCGGCGACTAAGGCAGGTCAGCCATGGCGAGGAGGGCGACGAGGGCGCGCCGTCCCAA 180
5685R-2.IR full 181 ATGGACGACGAGTTGGAACAGATGACCAAGGTGCATGGAGAAGCACCGGACGCGGAGGAG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5685R-2.in silico 181 ATGGACGACGAGTTGGAACAGATGACCAAGGTGCATGGAGAAGCACCGGACGCGGAGGAG 240
5685R-2.IR full 241 CTGCGCGAATGTAGCGAGGGTCTTGTCCTGCCACTCTGGATGCCGCAACGGAACATTTCG 300
|||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
5685R-2.in silico 241 CTGCGCGAATGTAGCGAGGGTCTTGTCCTGCCACTCTGGATGCCGCAACGCAACATTTCG 300
5685R-2.IR full 301 GTGGGTGACCGCCTCGTCCGCGGCTTTGTTTACTTCGTCCTACTGATCTACCTGTTTGTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5685R-2.in silico 301 GTGGGTGACCGCCTCGTCCGCGGCTTTGTTTACTTCGTCCTACTGATCTACCTGTTTGTG 360
5685R-2.IR full 361 GGTGTGTCCNTCATTGCGGATCGCTTCATGGCSGCCATCGAGGCAATCACATCGATTGAA 420
||||||||| |||||||||||||||||||||| |||||||||||||||||||||||||||
5685R-2.in silico 361 GGTGTGTCCATCATTGCGGATCGCTTCATGGCCGCCATCGAGGCAATCACATCGATTGAA 420
5685R-2.IR full 421 CGGGCGGTGGTCGTCAAGGGACCCAACAACACCAAGCAGGTGATGCACGTGCGCATCTGG 480
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
5685R-2.in silico 421 CGGGCGGTGGTGGTCAAGGGACCCAACAACACCAAGCAGGTGATGCACGTGCGCATCTGG 480
5685R-2.IR full 481 AACGAAACGGTGGCGAATCT 500
||||||||||||||||||||
5685R-2.in silico 481 AACGAAACGGTGGCGAATCT 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| ++ |
|
| VM |
 |
| ++ |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| |
|
|
| Dcompartment |
|
Penetrance |
| ++ |
|
|
|
|
