NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 5655R-1

Symbol Rsf1 Full Name Repressor splicing factor 1

CG No CG5655 Old CG No CG5655

Synonyms RSF1, CG5655, Rox21, rox21, unnamed, ROX21, Rsf1

Accession No (Link to NCBI) NM_057653.2

Inserted Chr. lll

Insertional Mutation semi-lethal

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGTCCAGCATGGGTGATCAGC

Primer Seq. 3'

aatctagaggtaccTGTCGCGTCCACCGCCGTAAA

Predicted Fragment Size

IR fragment full Seq

0001 TGTCCAGCAT GGGTGATCAG CGCGGGACAC GGGTGTATGT CGGCAATCTG ACCGACAAAG 
0061 TGAAAAAGGA TGATCTGGAG GGGGAGTTCA CAAAGTATGG CAAGCTGAAT TCGGTGTGGA 
0121 TAGCCTTCAA TCCGCCGGGA TTTGCGTTCG TCGAGTTCGA GCACCGCGAC GACGCCGAAA 
0181 AGGCGTGCGA CATACTGAAC GGATCCGAGC TGCTCGGCTC CCAgCTGCGC GTGNAGATCT 
0241 CAAANGGGcG NCCACGCCAG GGTANGGCGT GGCGGACCCA TGGACAGGGG CGGACGACGC 
0301 GGCGACTTTG GCCGGCACAG CATCACAAGC GGTGGTAGCG GCGGAGGCGG TTTNCGGCAG 
0361 CNCgGATCCA GCGGATCCTC AAGCCGGCAC ACGGAGCGGG GCTATAGCTC CGGCCGATCA 
0421 GGTGCAAGCA GCTATAATGG CAGAGAGGGC GGCGGCAgcG GCTTCAATCG CCGCGAGGTT 
0481 TACGGCGGTG GACGCGACA

in silico PCR Fragment

0001 TGTCCAGCAT GGGTGATCAG CGCGGGACAC GGGTGTATGT CGGCAATCTG ACCGACAAAG 
0061 TGAAAAAGGA TGATCTGGAG GGGGAGTTCA CAAAGTATGG CAAGCTGAAT TCGGTGTGGA 
0121 TAGCCTTCAA TCCGCCGGGA TTTGCGTTCG TCGAGTTCGA GCACCGCGAC GACGCCGAAA 
0181 AGGCGTGCGA CATACTGAAC GGATCCGAGC TGCTCGGCTC CCAGCTGCGC GTGGAGATCT 
0241 CAAAAGGGCG GCCACGCCAG GGTA-GGCGT GGCGGACCCA TGGACAGGGG CGGACGACGC 
0301 GGCGACTTTG GCCGGCACAG CATCACAAGC GGTGGTAGCG GCGGAGGCGG TTTCCGGCAG 
0361 CGCGGATCCA GCGGATCCTC AAGCCGGCAC ACGGAGCGGG GCTATAGCTC CGGCCGATCA 
0421 GGTGCAAGCA GCTATAATGG CAGAGAGGGC GGCGGCAGCG GCTTCAATCG CCGCGAGGTT 
0481 TACGGCGGTG GACGCGACA

Assemble Data


5655R-1.IR_full       1   TGTCCAGCATGGGTGATCAGCGCGGGACACGGGTGTATGTCGGCAATCTGACCGACAAAG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5655R-1.in silico     1   TGTCCAGCATGGGTGATCAGCGCGGGACACGGGTGTATGTCGGCAATCTGACCGACAAAG 60


5655R-1.IR_full       61  TGAAAAAGGATGATCTGGAGGGGGAGTTCACAAAGTATGGCAAGCTGAATTCGGTGTGGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5655R-1.in silico     61  TGAAAAAGGATGATCTGGAGGGGGAGTTCACAAAGTATGGCAAGCTGAATTCGGTGTGGA 120


5655R-1.IR_full       121 TAGCCTTCAATCCGCCGGGATTTGCGTTCGTCGAGTTCGAGCACCGCGACGACGCCGAAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5655R-1.in silico     121 TAGCCTTCAATCCGCCGGGATTTGCGTTCGTCGAGTTCGAGCACCGCGACGACGCCGAAA 180


5655R-1.IR_full       181 AGGCGTGCGACATACTGAACGGATCCGAGCTGCTCGGCTCCCAGCTGCGCGTGNAGATCT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
5655R-1.in silico     181 AGGCGTGCGACATACTGAACGGATCCGAGCTGCTCGGCTCCCAGCTGCGCGTGGAGATCT 240


5655R-1.IR_full       241 CAAANGGGCGNCCACGCCAGGGTANGGCGTGGCGGACCCATGGACAGGGGCGGACGACGC 300
                          |||| ||||| ||||||||||||| |||||||||||||||||||||||||||||||||||
5655R-1.in silico     241 CAAAAGGGCGGCCACGCCAGGGTA-GGCGTGGCGGACCCATGGACAGGGGCGGACGACGC 300


5655R-1.IR_full       301 GGCGACTTTGGCCGGCACAGCATCACAAGCGGTGGTAGCGGCGGAGGCGGTTTNCGGCAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
5655R-1.in silico     301 GGCGACTTTGGCCGGCACAGCATCACAAGCGGTGGTAGCGGCGGAGGCGGTTTCCGGCAG 360


5655R-1.IR_full       361 CNCGGATCCAGCGGATCCTCAAGCCGGCACACGGAGCGGGGCTATAGCTCCGGCCGATCA 420
                          | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5655R-1.in silico     361 CGCGGATCCAGCGGATCCTCAAGCCGGCACACGGAGCGGGGCTATAGCTCCGGCCGATCA 420


5655R-1.IR_full       421 GGTGCAAGCAGCTATAATGGCAGAGAGGGCGGCGGCAGCGGCTTCAATCGCCGCGAGGTT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5655R-1.in silico     421 GGTGCAAGCAGCTATAATGGCAGAGAGGGCGGCGGCAGCGGCTTCAATCGCCGCGAGGTT 480


5655R-1.IR_full       481 TACGGCGGTGGACGCGACA 499
                          |||||||||||||||||||
5655R-1.in silico     481 TACGGCGGTGGACGCGACA 499

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	480	0	0	3	NM_057653.2	CG5655-RA (Rsf1), mRNA
0	0	4	2	19	NM_169258.1	CG9755-RA, transcript variant A (pum), mRNA
0	0	4	2	19	NM_169259.1	CG9755-RD, transcript variant D (pum), mRNA
0	0	4	2	19	NM_079561.2	CG9755-RC, transcript variant C (pum), mRNA
0	0	4	2	12	NM_169260.1	CG9755-RB, transcript variant B (pum), mRNA
0	0	3	7	15	NM_132536.1	CG1840-RA (CG1840), mRNA
0	0	3	6	7	NM_167881.1	CG12085-RC, transcript variant C (pUf68), mRNA
0	0	3	6	7	NM_167882.1	CG12085-RD, transcript variant D (pUf68), mRNA
0	0	3	6	7	NM_080384.2	CG12085-RA, transcript variant A (pUf68), mRNA
0	0	3	6	7	NM_167880.1	CG12085-RB, transcript variant B (pUf68), mRNA
0	0	3	6	5	NM_140011.2	CG5660-RA (CG5660), mRNA
0	0	3	3	2	NM_132778.1	CG9095-RA (CG9095), mRNA
0	0	2	6	8	NM_206564.1	CG5794-RE, transcript variant E (CG5794), mRNA
0	0	2	6	8	NM_143018.2	CG5794-RD, transcript variant D (CG5794), mRNA
0	0	2	6	8	NM_170158.1	CG5794-RB, transcript variant B (CG5794), mRNA
0	0	2	2	5	NM_001014571.1	CG33556-RA (form3), mRNA
0	0	2	2	2	NM_134943.1	CG3213-RA (CG3213), mRNA
0	0	2	2	2	NM_138206.1	CG3371-RA (CG3371), mRNA
0	0	1	11	21	NM_079093.2	CG9888-RA (Fib), mRNA
0	0	1	9	16	NM_143742.2	CG5812-RA (GCR(ich)), mRNA
0	0	1	5	32	NM_132412.1	CG9817-RA (CG9817), mRNA
0	0	1	3	17	NM_134516.2	CG32529-RA, transcript variant A (CG32529), mRNA
0	0	1	3	14	NM_167688.1	CG32529-RC, transcript variant C (CG32529), mRNA
0	0	1	3	14	NM_001038766.1	CG32529-RD, transcript variant D (CG32529), mRNA
0	0	1	3	5	NM_141936.1	CG6525-RA (CG6525), mRNA
0	0	1	3	4	NM_166239.2	CG4878-RA, transcript variant A (eIF3-S9), mRNA
0	0	1	3	3	NM_001032231.1	CG18003-RA, transcript variant A (CG18003), mRNA
0	0	1	3	3	NM_001032232.1	CG11919-RA, transcript variant A (CG11919), mRNA
0	0	0	13	11	NM_169730.1	CG10388-RC, transcript variant C (Ubx), mRNA
0	0	0	13	11	NM_080504.2	CG10388-RA, transcript variant A (Ubx), mRNA

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