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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 5597R-1 
 Symbol CG5597  Full Name CG5597 
 CG No CG5597  Old CG No CG5597 
 Synonyms CT17698, CG5597 
 Accession No (Link to NCBI) NM_137997.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGGATACCCTGCTGATCGCC 
 Primer Seq. 3' 
aatctagaggtaccCTGGGATACACGTTCGTCACG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGGATACCC TGCTGATCGC CATTCTGGCC ATCGGACTGC TCCACAGAGA TGCGCTATGC 
0061 TATCCCACGA GGGATGAGTC CGAGGACAAG ATTATAGTCC TTCAGAACGA GGAGATGGAG 
0121 CCCACGATCC TGGACTGCGA CTACGAGGTG GAGGAGAGCC CGAAGTTCAT CACCGTCAAA 
0181 TGGTACAGAG ACGACAAGTC CATATACCAG TGGATCTTCG GAACACCTCC ATATGCCATC 
0241 CCCGAGTTCA GGAACGAGAT TGACAGCACC TATGAGAGCT CCACGGAGCC GAGCAAGCAG 
0301 TACAGCTCGC TGGCTTTGAT CAACCCCACG ATCGCCACCA CCGGCGACTA CAAGTGCGTG 
0361 GTGCAGACCT CGCTGAACAC CTTCTCCAGC CACCAGAGGG TTCAGGTCAT AGACCTGAGG 
0421 AACTACACCC TGGAGCTGTC CCACAAGACG ATCCACAATG AGACGCAGCT GAACTGCACC 
0481 GTGACGAACG TGTATCCCAG  
 in silico PCR Fragment 
0001 ATGGATACCC TGCTGATCGC CATTCTGGCC ATCGGACTGC TCCACAGAGA TGCGCTATGC 
0061 TATCCCACGA GGGATGAGTC CGAGGACAAG ATTATAGTCC TTCAGAACGA GGAGATGGAG 
0121 CCCACGATCC TGGACTGCGA CTACGAGGTG GAGGAGAGCC CGAAGTTCAT CACCGTCAAA 
0181 TGGTACAGAG ACGACAAGTC CATATACCAG TGGATCTTCG GAACACCTCC ATATGCCATC 
0241 CCCGAGTTCA GGAACGAGAT TGACAGCACC TATGAGAGCT CCACGGAGCC GAGCAAGCAG 
0301 TACAGCTCGC TGGCTTTGAT CAACCCCACG ATCGCCACCA CCGGCGACTA CAAGTGCGTG 
0361 GTGCAGACCT CGCTGAACAC CTTCTCCAGC CACCAGAGGG TTCAGGTCAT AGACCTGAGG 
0421 AACTACACCC TGGAGCTGTC CCACAAGACG ATCCACAATG AGACGCAGCT GAACTGCACC 
0481 GTGACGAACG TGTATCCCAG  
 Assemble Data 

5597R-1.IR_full       1   ATGGATACCCTGCTGATCGCCATTCTGGCCATCGGACTGCTCCACAGAGATGCGCTATGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5597R-1.in silico     1   ATGGATACCCTGCTGATCGCCATTCTGGCCATCGGACTGCTCCACAGAGATGCGCTATGC 60


5597R-1.IR_full       61  TATCCCACGAGGGATGAGTCCGAGGACAAGATTATAGTCCTTCAGAACGAGGAGATGGAG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5597R-1.in silico     61  TATCCCACGAGGGATGAGTCCGAGGACAAGATTATAGTCCTTCAGAACGAGGAGATGGAG 120


5597R-1.IR_full       121 CCCACGATCCTGGACTGCGACTACGAGGTGGAGGAGAGCCCGAAGTTCATCACCGTCAAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5597R-1.in silico     121 CCCACGATCCTGGACTGCGACTACGAGGTGGAGGAGAGCCCGAAGTTCATCACCGTCAAA 180


5597R-1.IR_full       181 TGGTACAGAGACGACAAGTCCATATACCAGTGGATCTTCGGAACACCTCCATATGCCATC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5597R-1.in silico     181 TGGTACAGAGACGACAAGTCCATATACCAGTGGATCTTCGGAACACCTCCATATGCCATC 240


5597R-1.IR_full       241 CCCGAGTTCAGGAACGAGATTGACAGCACCTATGAGAGCTCCACGGAGCCGAGCAAGCAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5597R-1.in silico     241 CCCGAGTTCAGGAACGAGATTGACAGCACCTATGAGAGCTCCACGGAGCCGAGCAAGCAG 300


5597R-1.IR_full       301 TACAGCTCGCTGGCTTTGATCAACCCCACGATCGCCACCACCGGCGACTACAAGTGCGTG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5597R-1.in silico     301 TACAGCTCGCTGGCTTTGATCAACCCCACGATCGCCACCACCGGCGACTACAAGTGCGTG 360


5597R-1.IR_full       361 GTGCAGACCTCGCTGAACACCTTCTCCAGCCACCAGAGGGTTCAGGTCATAGACCTGAGG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5597R-1.in silico     361 GTGCAGACCTCGCTGAACACCTTCTCCAGCCACCAGAGGGTTCAGGTCATAGACCTGAGG 420


5597R-1.IR_full       421 AACTACACCCTGGAGCTGTCCCACAAGACGATCCACAATGAGACGCAGCTGAACTGCACC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5597R-1.in silico     421 AACTACACCCTGGAGCTGTCCCACAAGACGATCCACAATGAGACGCAGCTGAACTGCACC 480


5597R-1.IR_full       481 GTGACGAACGTGTATCCCAG 500
                          ||||||||||||||||||||
5597R-1.in silico     481 GTGACGAACGTGTATCCCAG 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_137997.2  CG5597-RA (CG5597), mRNA 
0   0  4  5  3  NM_164531.1  CG3332-RB, transcript variant B (CG3332), mRNA 
0   0  4  5  3  NM_134914.2  CG3332-RA, transcript variant A (CG3332), mRNA 
0   0  4  2  4  NM_169709.1  CG6889-RB, transcript variant B (tara), mRNA 
0   0  4  2  4  NM_080255.2  CG6889-RA, transcript variant A (tara), mRNA 
0   0  2  6  4  NM_169130.1  CG1148-RA, transcript variant A (Osi2), mRNA 
0   0  2  6  4  NM_141364.1  CG1148-RB, transcript variant B (Osi2), mRNA 
0   0  2  2  4  NM_136793.1  CG13229-RA (CG13229), mRNA 
0   0  1  5  8  NM_078601.2  CG9533-RA (rut), mRNA 
0   0  1  5  5  NM_132179.2  CG1402-RA (CG1402), mRNA 
0   0  1  5  2  NM_137777.1  CG13494-RA (CG13494), mRNA 
0   0  1  3  4  NM_057624.2  CG4698-RA (Wnt4), mRNA 
0   0  1  3  2  NM_133160.1  CG12233-RA, transcript variant A (l(1)G0156), mRNA 
0   0  1  3  2  NM_167657.1  CG12233-RB, transcript variant B (l(1)G0156), mRNA 
0   0  1  3  2  NM_079431.2  CG9739-RA, transcript variant A (fz2), mRNA 
0   0  1  3  2  NM_168787.1  CG9739-RB, transcript variant B (fz2), mRNA 
0   0  1  2  3  NM_136585.1  CG13745-RA (CG13745), mRNA 
0   0  1  2  3  NM_140343.2  CG17667-RA, transcript variant A (CG17667), mRNA 
0   0  1  2  3  NM_168516.1  CG17667-RB, transcript variant B (CG17667), mRNA 
0   0  0  6  6  NM_141549.1  CG8007-RB, transcript variant B (CG8007), mRNA 
0   0  0  5  14  NM_001043067.1  CG33141-RB, transcript variant B (sns), mRNA 
0   0  0  5  14  NM_176106.1  CG33141-RA, transcript variant A (sns), mRNA 
0   0  0  5  10  NM_141231.3  CG31534-RA, transcript variant A (CG31534), mRNA 
0   0  0  5  10  NM_176400.1  CG31534-RB, transcript variant B (CG31534), mRNA 
0   0  0  5  9  NM_175983.1  CG8222-RC, transcript variant C (Pvr), mRNA 
0   0  0  5  9  NM_205927.1  CG8222-RD, transcript variant D (Pvr), mRNA 
0   0  0  5  9  NM_164801.1  CG8222-RB, transcript variant B (Pvr), mRNA 
0   0  0  5  9  NM_078785.2  CG8222-RA, transcript variant A (Pvr), mRNA 
0   0  0  5  9  NM_205925.1  CG8222-RF, transcript variant F (Pvr), mRNA 
0   0  0  5  9  NM_205926.1  CG8222-RE, transcript variant E (Pvr), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
+- 
DC
+- 
DM
+- 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
+ 
DC
+ 
DM
++ 
VM
+ 
VB
 
Dcompartment   Penetrance
+     

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