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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5562R-1 |
| Symbol |
gbb |
Full Name |
glass bottom boat |
| CG No |
CG5562 |
Old CG No |
CG5562 |
| Synonyms |
Gbb, gbb-60A, Dm-GBB, TGF-b, tgfb-60A, 60A, gcn, l(2)60A-J, Gbb-60A, TGFbeta-60A, Tgfbeta-60A, Tgfb-60, SixtyA, vgr/60A, CG5562, gbb, GBB, BMP |
| Accession No (Link to NCBI) |
NM_057992.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Graca FA, Rai M, Hunt LC, Stephan A, Wang YD, Gordon B, Wang R, Quarato G, Xu B, Fan Y, Labelle M, Demontis F.
The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophy.
Nat Commun (2022) 13(1) 2370
[
PubMed ID = 35501350
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Wang M, Chen PY, Wang CH, Lai TT, Tsai PI, Cheng YJ, Kao HH, Chien CT.
Dbo/Henji Modulates Synaptic dPAK to Gate Glutamate Receptor Abundance and Postsynaptic Response.
PLoS Genet (2016) 12(10) e1006362
[
PubMed ID = 27736876
]
[
RRC reference
]
Yamanaka N, Marqués G, O'Connor MB.
Vesicle-Mediated Steroid Hormone Secretion in Drosophila melanogaster.
Cell (2015) 163(4) 907-19
[
PubMed ID = 26544939
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Sato T, Ogata J, Niki Y.
BMP and Hh signaling affects primordial germ cell division in Drosophila.
Zoolog Sci (2010) 27(10) 804-10
[
PubMed ID = 20887178
]
[
RRC reference
]
Lesch C, Jo J, Wu Y, Fish GS, Galko MJ.
A targeted UAS-RNAi screen in Drosophila larvae identifies wound closure genes regulating distinct cellular processes.
Genetics (2010) 186(3) 943-57
[
PubMed ID = 20813879
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGTGCTGAGCGAGGACGACAA |
| Primer Seq. 3' |
aatctagaggtaccTGGCGTATACCGTGATGGTGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGTGCTGAGC GAGGACGACA AGCTGGACGT CTCGTACGAG ATCCTCGAGT TCCTGGGCAT
0061 CGcCGAACGG CCGACGCACC TGAGCAGCCA CCAGTTGTCG CTGAGGAAGT CGGCTCCCAA
0121 GTTCCTGCTG GACGTCTANC CACCGCATCA CGGCGGAGGA GGGTCTCAGC GATCAGGATG
0181 AGGACGACGC CTACGAACGC GGCCATCGGT CCAGGAGGAG CGCCGACCTC GAGGAGGATG
0241 AGGCCGAGCA GCAGAAGAAC TTCATCACCG ACCTAGACAA GCGGGCCATC GACGAGAGCG
0301 ACATCATCAT GACCTTCCTG AACAAGCGCC ACCACAATGT GGACGAACTG CGTCACGNAG
0361 CACGGCCGTC GCCTGTGGTT CGACGTCTCC AACGTGCCCA ACGACAACTA CCTGGTTATG
0421 GCCGAGCTGC GCATCTATCA GAACGCCAAC GAGGGCAAGT GGCTGACCGC CAACAGGGAG
0481 TTCACCATCA CGGTATACGC CA |
| in silico PCR Fragment |
0001 AGTGCTGAGC GAGGACGACA AGCTGGACGT CTCGTACGAG ATCCTCGAGT TCCTGGGCAT
0061 CGCCGAACGG CCGACGCACC TGAGCAGCCA CCAGTTGTCG CTGAGGAAGT CGGCTCCCAA
0121 GTTCCTGCTG GACGTCTA-C CACCGCATCA CGGCGGAGGA GGGTCTCAGC GATCAGGATG
0181 AGGACGACGA CTACGAACGC GGCCATCGGT CCAGGAGGAG CGCCGACCTC GAGGAGGATG
0241 AGGGCGAGCA GCAGAAGAAC TTCATCACCG ACCTGGACAA GCGGGCCATC GACGAGAGCG
0301 ACATCATCAT GACCTTCCTG AACAAGCGCC ACCACAATGT GGACGAACTG CGTCACG-AG
0361 CACGGCCGTC GCCTGTGGTT CGACGTCTCC AACGTGCCCA ACGACAACTA CCTGGTGATG
0421 GCCGAGCTGC GCATCTATCA GAACGCCAAC GAGGGCAAGT GGCTGACCGC CAACAGGGAG
0481 TTCACCATCA CGGTATACGC CA |
| Assemble Data |
5562R-1.IR_full 1 AGTGCTGAGCGAGGACGACAAGCTGGACGTCTCGTACGAGATCCTCGAGTTCCTGGGCAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5562R-1.in silico 1 AGTGCTGAGCGAGGACGACAAGCTGGACGTCTCGTACGAGATCCTCGAGTTCCTGGGCAT 60
5562R-1.IR_full 61 CGCCGAACGGCCGACGCACCTGAGCAGCCACCAGTTGTCGCTGAGGAAGTCGGCTCCCAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5562R-1.in silico 61 CGCCGAACGGCCGACGCACCTGAGCAGCCACCAGTTGTCGCTGAGGAAGTCGGCTCCCAA 120
5562R-1.IR_full 121 GTTCCTGCTGGACGTCTANCCACCGCATCACGGCGGAGGAGGGTCTCAGCGATCAGGATG 180
|||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
5562R-1.in silico 121 GTTCCTGCTGGACGTCTA-CCACCGCATCACGGCGGAGGAGGGTCTCAGCGATCAGGATG 180
5562R-1.IR_full 181 AGGACGACGCCTACGAACGCGGCCATCGGTCCAGGAGGAGCGCCGACCTCGAGGAGGATG 240
||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
5562R-1.in silico 181 AGGACGACGACTACGAACGCGGCCATCGGTCCAGGAGGAGCGCCGACCTCGAGGAGGATG 240
5562R-1.IR_full 241 AGGCCGAGCAGCAGAAGAACTTCATCACCGACCTAGACAAGCGGGCCATCGACGAGAGCG 300
||| |||||||||||||||||||||||||||||| |||||||||||||||||||||||||
5562R-1.in silico 241 AGGGCGAGCAGCAGAAGAACTTCATCACCGACCTGGACAAGCGGGCCATCGACGAGAGCG 300
5562R-1.IR_full 301 ACATCATCATGACCTTCCTGAACAAGCGCCACCACAATGTGGACGAACTGCGTCACGNAG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
5562R-1.in silico 301 ACATCATCATGACCTTCCTGAACAAGCGCCACCACAATGTGGACGAACTGCGTCACG-AG 360
5562R-1.IR_full 361 CACGGCCGTCGCCTGTGGTTCGACGTCTCCAACGTGCCCAACGACAACTACCTGGTTATG 420
|||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
5562R-1.in silico 361 CACGGCCGTCGCCTGTGGTTCGACGTCTCCAACGTGCCCAACGACAACTACCTGGTGATG 420
5562R-1.IR_full 421 GCCGAGCTGCGCATCTATCAGAACGCCAACGAGGGCAAGTGGCTGACCGCCAACAGGGAG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5562R-1.in silico 421 GCCGAGCTGCGCATCTATCAGAACGCCAACGAGGGCAAGTGGCTGACCGCCAACAGGGAG 480
5562R-1.IR_full 481 TTCACCATCACGGTATACGCCA 502
||||||||||||||||||||||
5562R-1.in silico 481 TTCACCATCACGGTATACGCCA 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_057992.2 |
CG5562-RA (gbb), mRNA |
| 0.82 |
4 |
2 |
3 |
5 |
NM_169261.1 |
CG9745-RB, transcript variant B (D1), mRNA |
| 0.82 |
4 |
2 |
3 |
5 |
NM_079562.1 |
CG9745-RA, transcript variant A (D1), mRNA |
| 0.82 |
4 |
2 |
3 |
5 |
NM_169262.1 |
CG9745-RC, transcript variant C (D1), mRNA |
| 0.62 |
3 |
3 |
5 |
9 |
NM_167348.1 |
CG32638-RA (CG32638), mRNA |
| 0 |
0 |
4 |
3 |
16 |
NM_079712.2 |
CG18402-RA (InR), mRNA |
| 0 |
0 |
3 |
5 |
3 |
NM_135487.2 |
CG4709-RA (CG4709), mRNA |
| 0 |
0 |
3 |
3 |
6 |
NM_135032.1 |
CG12787-RC, transcript variant C (hoe1), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_175967.1 |
CG12787-RE, transcript variant E (hoe1), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_175966.1 |
CG12787-RD, transcript variant D (hoe1), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_175968.1 |
CG12787-RF, transcript variant F (hoe1), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_164603.1 |
CG12787-RB, transcript variant B (hoe1), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_135031.3 |
CG12787-RA, transcript variant A (hoe1), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_169487.1 |
CG31342-RA (CG31342), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_206242.1 |
CG32306-RD, transcript variant D (CG32306), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_139464.2 |
CG32306-RB, transcript variant B (CG32306), mRNA |
| 0 |
0 |
2 |
8 |
4 |
NM_141261.2 |
CG1115-RA (CG1115), mRNA |
| 0 |
0 |
2 |
7 |
10 |
NM_134690.2 |
CG2807-RA (CG2807), mRNA |
| 0 |
0 |
2 |
5 |
7 |
NM_078664.2 |
CG5870-RA (beta-Spec), mRNA |
| 0 |
0 |
2 |
4 |
11 |
NM_169423.2 |
CG17342-RB, transcript variant B (Lk6), mRNA |
| 0 |
0 |
2 |
4 |
11 |
NM_143729.2 |
CG17342-RA, transcript variant A (Lk6), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_167216.1 |
CG15312-RC, transcript variant C (CG15312), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_167215.1 |
CG15312-RB, transcript variant B (CG15312), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_130629.2 |
CG3457-RA (CG3457), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_132373.2 |
CG15312-RA, transcript variant A (CG15312), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_078643.2 |
CG4211-RA, transcript variant A (nonA), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_001014747.1 |
CG4211-RC, transcript variant C (nonA), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_167505.1 |
CG4211-RB, transcript variant B (nonA), mRNA |
| 0 |
0 |
2 |
2 |
12 |
NM_058149.3 |
CG3352-RA (ft), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_139655.1 |
CG13721-RA (CG13721), mRNA |
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