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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5554R-1 |
| Symbol |
CG5554 |
Full Name |
CG5554 |
| CG No |
CG5554 |
Old CG No |
CG5554 |
| Synonyms |
CG5554 |
| Accession No (Link to NCBI) |
NM_137994.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Fan W, Lam SM, Xin J, Yang X, Liu Z, Liu Y, Wang Y, Shui G, Huang X.
Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition.
PLoS Genet (2017) 13(3) e1006664
[
PubMed ID = 28273089
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
balanced with Green Balancer. CyO, P{w[+mC]=ActGFP}JMR1
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACTGCAGCCAGGTGGCAAGTT |
| Primer Seq. 3' |
aatctagaggtaccCGAAGATGGTGGCGATGGCGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ACTGCAGCCA GGTGGcAAGT TAATCGAGTT GGACGAGGAC AACTGGCACC TGATGCTCCA
0061 GGGCGAATGG ATGATCGAGT TCTTTGCTCC ATGGTGCCCG GCCTGCAAGA ACCTGGCTCC
0121 CACCTgGGAG CGGTTTGCCC GCGTGGCCAA GGATGTCCAG GTGCAGGTGG CCAAAATCGA
0181 CGTGACAACC TCGCCCTCGC TGAGTGGACG CTTCTTTGTC ACCGCCCTCC CCACCATCTA
0241 CCACGTAAAA GATGGCGAGT TCCGGCAGTA TCGTGGGGCC CGTGATGGCG ACGCCCTCCT
0301 CTACTTCGTG AAGAAGCAGC AGTGGCAGAG CATCGAACCG CTGTCCGCCT GGAAGAAGCC
0361 CGACACCACC CACATGTCCG TGCTGTCCTA CTTCTTCAAG CTTTCGCACA CTCTGAAGGA
0421 CTTCAACGGG CGCCTGCAGG AGGAATACGG ACTGCCCACG TGGGGCTCCT ACGCCCTCTT
0481 CGCCATCGCC ATCCATCTTC G |
| in silico PCR Fragment |
0001 ACTGCAGCCA GGTGGCAAGT TAATCGAGTT GGACGAGGAC AACTGGCACC TGATGCTCCA
0061 GGGCGAATGG ATGATCGAGT TCTTTGCTCC ATGGTGCCCG GCCTGCAAGA ACCTGGCTCC
0121 CACCTGGGAG CGGTTTGCCC GCGTGGCCAA GGATGTCCAG GTGCAGGTGG CCAAAATCGA
0181 CGTGACAACC TCGCCCTCGC TGAGTGGACG CTTCTTTGTC ACCGCCCTCC CCACCATCTA
0241 CCACGTAAAA GATGGCGAGT TCCGGCAGTA TCGTGGGGCC CGTGATGGCG ACGCCCTCCT
0301 CTACTTCGTG AAGAAGCAGC AGTGGCAGAG CATCGAACCG CTGTCCGCCT GGAAGAAGCC
0361 CGACACCACC CACATGTCCG TGCTGTCCTA CTTCTTCAAG CTTTCGCACA CTCTGAAGGA
0421 CTTCAACGGG CGCCTGCAGG AGGAATACGG ACTGCCCACG TGGGGCTCCT ACGCCCTCTT
0481 CGCCATCGCC A-CCATCTTC G |
| Assemble Data |
5554R-1.IR_full 1 ACTGCAGCCAGGTGGCAAGTTAATCGAGTTGGACGAGGACAACTGGCACCTGATGCTCCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5554R-1.in silico 1 ACTGCAGCCAGGTGGCAAGTTAATCGAGTTGGACGAGGACAACTGGCACCTGATGCTCCA 60
5554R-1.IR_full 61 GGGCGAATGGATGATCGAGTTCTTTGCTCCATGGTGCCCGGCCTGCAAGAACCTGGCTCC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5554R-1.in silico 61 GGGCGAATGGATGATCGAGTTCTTTGCTCCATGGTGCCCGGCCTGCAAGAACCTGGCTCC 120
5554R-1.IR_full 121 CACCTGGGAGCGGTTTGCCCGCGTGGCCAAGGATGTCCAGGTGCAGGTGGCCAAAATCGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5554R-1.in silico 121 CACCTGGGAGCGGTTTGCCCGCGTGGCCAAGGATGTCCAGGTGCAGGTGGCCAAAATCGA 180
5554R-1.IR_full 181 CGTGACAACCTCGCCCTCGCTGAGTGGACGCTTCTTTGTCACCGCCCTCCCCACCATCTA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5554R-1.in silico 181 CGTGACAACCTCGCCCTCGCTGAGTGGACGCTTCTTTGTCACCGCCCTCCCCACCATCTA 240
5554R-1.IR_full 241 CCACGTAAAAGATGGCGAGTTCCGGCAGTATCGTGGGGCCCGTGATGGCGACGCCCTCCT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5554R-1.in silico 241 CCACGTAAAAGATGGCGAGTTCCGGCAGTATCGTGGGGCCCGTGATGGCGACGCCCTCCT 300
5554R-1.IR_full 301 CTACTTCGTGAAGAAGCAGCAGTGGCAGAGCATCGAACCGCTGTCCGCCTGGAAGAAGCC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5554R-1.in silico 301 CTACTTCGTGAAGAAGCAGCAGTGGCAGAGCATCGAACCGCTGTCCGCCTGGAAGAAGCC 360
5554R-1.IR_full 361 CGACACCACCCACATGTCCGTGCTGTCCTACTTCTTCAAGCTTTCGCACACTCTGAAGGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5554R-1.in silico 361 CGACACCACCCACATGTCCGTGCTGTCCTACTTCTTCAAGCTTTCGCACACTCTGAAGGA 420
5554R-1.IR_full 421 CTTCAACGGGCGCCTGCAGGAGGAATACGGACTGCCCACGTGGGGCTCCTACGCCCTCTT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5554R-1.in silico 421 CTTCAACGGGCGCCTGCAGGAGGAATACGGACTGCCCACGTGGGGCTCCTACGCCCTCTT 480
5554R-1.IR_full 481 CGCCATCGCCATCCATCTTCG 501
||||||||||| |||||||||
5554R-1.in silico 481 CGCCATCGCCA-CCATCTTCG 501
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_137994.2 |
CG5554-RA (CG5554), mRNA |
| 0.41 |
2 |
4 |
3 |
12 |
NM_132514.2 |
CG1837-RA (CG1837), mRNA |
| 0 |
0 |
4 |
5 |
4 |
NM_135948.2 |
CG5809-RA (CaBP1), mRNA |
| 0 |
0 |
3 |
5 |
2 |
NM_139466.3 |
CG16761-RA (Cyp4d20), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_132155.2 |
CG4617-RA (CG4617), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_057741.3 |
CG4720-RA, transcript variant A (Pk92B), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_206518.1 |
CG4720-RB, transcript variant B (Pk92B), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_137828.1 |
CG10972-RA (ppk12), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_143320.2 |
CG18437-RA (CG18437), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_142645.2 |
CG4141-RA, transcript variant A (Pi3K92E), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_169910.1 |
CG4141-RB, transcript variant B (Pi3K92E), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_168994.1 |
CG14648-RB, transcript variant B (CG14648), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_141209.2 |
CG14648-RA, transcript variant A (CG14648), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_135257.1 |
CG4496-RA (CG4496), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_141397.2 |
CG1021-RB, transcript variant B (CG1021), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_169141.1 |
CG1021-RA, transcript variant A (CG1021), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_137459.2 |
CG10916-RA (CG10916), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_169267.1 |
CG8121-RC, transcript variant C (CG8121), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_169266.1 |
CG8121-RB, transcript variant B (CG8121), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_141626.1 |
CG8121-RA, transcript variant A (CG8121), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_079611.2 |
CG18550-RA (yellow-f), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_139411.1 |
CG1919-RA (CG1919), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_165207.1 |
CG31748-RA (Gr36c), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_165951.1 |
CG17579-RB, transcript variant B (sca), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_057362.2 |
CG17579-RA, transcript variant A (sca), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_132436.2 |
CG15208-RA (CG15208), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_168554.1 |
CG32130-RD, transcript variant D (stv), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_168551.1 |
CG32130-RA, transcript variant A (stv), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_168553.1 |
CG32130-RB, transcript variant B (stv), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_168552.1 |
CG32130-RC, transcript variant C (stv), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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