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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 5452R-4 
 Symbol dnk  Full Name deoxyribonucleoside kinase 
 CG No CG5452  Old CG No CG5452 
 Synonyms DmdNK, Dm-dNK, dNK, DM-dNK, CG5452, dnk 
 Accession No (Link to NCBI) NM_079675.2 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees male lethal, female semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAAAGGGGACCAAGTACGCCGA 
 Primer Seq. 3' 
aatctagaggtaccGCACGCAGCTCTCCTCAGAAC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AAAGGGGACC AAGTACGCCG AgGGCACCCA GCCCTTCACC GTCCTCATCG AGGGCAACAT 
0061 CGGCAGCGGG AAGACCACGT ATTTGAACCA CTTCGAGAAG TACAAGAACG ACATTTGCCT 
0121 GCTGACCGAG CCCGTCGAGA AGTGGCGCAA CGTCAACGGG GTAAATCTGC TGGAGCTGAT 
0181 GTACAAAGAT CCCAAGAAGT GGGCCATGCC CTTTCAGAGT TATGTCACGC TGACCATGCT 
0241 GCAGTCGCAC ACCGCCCCAA CCAACAAGAA GCTAAAAATA ATGGAGCGCT CCATTTTTAG 
0301 CGCNTCGCTA TTGCTTCGTG GAGAACATGC GACGAAACGG CTCGCTGGAG CAGGGCATGT 
0361 ACAATACGCT GGAGGAGTGG TACAAGTTCA TCGAAGAGTC CATTCACGTG CAGGCGGACC 
0421 TCATCATATA TCTGCGCACC TCGCCGGAGG TGGCGTACGA ACGCATCCGG CAGCGGGCTC 
0481 GTTCTGAGGA GAGCTGCGTG C 
 in silico PCR Fragment 
0001 AAAGGGGACC AAGTACGCCG AGGGCACCCA GCCCTTCACC GTCCTCATCG AGGGCAACAT 
0061 CGGCAGCGGG AAGACCACGT ATTTGAACCA CTTCGAGAAG TACAAGAACG ACATTTGCCT 
0121 GCTGACCGAG CCCGTCGAGA AGTGGCGCAA CGTCAACGGG GTAAATCTGC TGGAGCTGAT 
0181 GTACAAAGAT CCCAAGAAGT GGGCCATGCC CTTTCAGAGT TATGTCACGC TGACCATGCT 
0241 GCAGTCGCAC ACCGCCCCAA CCAACAAGAA GCTAAAAATA ATGGAGCGCT CCATTTTTAG 
0301 CGC-TCGCTA TTGCTTCGTG GAGAACATGC GACGAAACGG CTCGCTGGAG CAGGGCATGT 
0361 ACAATACGCT GGAGGAGTGG TACAAGTTCA TCGAAGAGTC CATTCACGTG CAGGCGGACC 
0421 TCATCATATA TCTGCGCACC TCGCCGGAGG TGGCGTACGA ACGCATCCGG CAGCGGGCTC 
0481 GTTCTGAGGA GAGCTGCGTG C 
 Assemble Data 

5452R-4.IR_full       1   AAAGGGGACCAAGTACGCCGAGGGCACCCAGCCCTTCACCGTCCTCATCGAGGGCAACAT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5452R-4.in silico     1   AAAGGGGACCAAGTACGCCGAGGGCACCCAGCCCTTCACCGTCCTCATCGAGGGCAACAT 60


5452R-4.IR_full       61  CGGCAGCGGGAAGACCACGTATTTGAACCACTTCGAGAAGTACAAGAACGACATTTGCCT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5452R-4.in silico     61  CGGCAGCGGGAAGACCACGTATTTGAACCACTTCGAGAAGTACAAGAACGACATTTGCCT 120


5452R-4.IR_full       121 GCTGACCGAGCCCGTCGAGAAGTGGCGCAACGTCAACGGGGTAAATCTGCTGGAGCTGAT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5452R-4.in silico     121 GCTGACCGAGCCCGTCGAGAAGTGGCGCAACGTCAACGGGGTAAATCTGCTGGAGCTGAT 180


5452R-4.IR_full       181 GTACAAAGATCCCAAGAAGTGGGCCATGCCCTTTCAGAGTTATGTCACGCTGACCATGCT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5452R-4.in silico     181 GTACAAAGATCCCAAGAAGTGGGCCATGCCCTTTCAGAGTTATGTCACGCTGACCATGCT 240


5452R-4.IR_full       241 GCAGTCGCACACCGCCCCAACCAACAAGAAGCTAAAAATAATGGAGCGCTCCATTTTTAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5452R-4.in silico     241 GCAGTCGCACACCGCCCCAACCAACAAGAAGCTAAAAATAATGGAGCGCTCCATTTTTAG 300


5452R-4.IR_full       301 CGCNTCGCTATTGCTTCGTGGAGAACATGCGACGAAACGGCTCGCTGGAGCAGGGCATGT 360
                          ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5452R-4.in silico     301 CGC-TCGCTATTGCTTCGTGGAGAACATGCGACGAAACGGCTCGCTGGAGCAGGGCATGT 360


5452R-4.IR_full       361 ACAATACGCTGGAGGAGTGGTACAAGTTCATCGAAGAGTCCATTCACGTGCAGGCGGACC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5452R-4.in silico     361 ACAATACGCTGGAGGAGTGGTACAAGTTCATCGAAGAGTCCATTCACGTGCAGGCGGACC 420


5452R-4.IR_full       421 TCATCATATATCTGCGCACCTCGCCGGAGGTGGCGTACGAACGCATCCGGCAGCGGGCTC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5452R-4.in silico     421 TCATCATATATCTGCGCACCTCGCCGGAGGTGGCGTACGAACGCATCCGGCAGCGGGCTC 480


5452R-4.IR_full       481 GTTCTGAGGAGAGCTGCGTGC 501
                          |||||||||||||||||||||
5452R-4.in silico     481 GTTCTGAGGAGAGCTGCGTGC 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  2  4  2  NM_079675.2  CG5452-RA (dnk), mRNA 
0.41   2  2  2  2  NM_130516.2  CG3704-RA (CG3704), mRNA 
0.2   1  3  2  4  NM_001043031.1  CG17800-RP, transcript variant P (Dscam), mRNA 
0.2   1  3  2  4  NM_206047.1  CG17800-RE, transcript variant E (Dscam), mRNA 
0.2   1  3  2  4  NM_001043047.1  CG17800-RH, transcript variant H (Dscam), mRNA 
0.2   1  2  4  7  NM_001043015.1  CG17800-PAX (Dscam), mRNA 
0.2   1  2  4  6  NM_001043032.1  CG17800-PAY (Dscam), mRNA 
0.2   1  2  4  6  NM_001043017.1  CG17800-PAW (Dscam), mRNA 
0.2   1  2  4  6  NM_001043039.1  CG17800-PAV (Dscam), mRNA 
0.2   1  2  2  6  NM_001043030.1  CG17800-RX, transcript variant X (Dscam), mRNA 
0.2   1  2  2  5  NM_001043059.1  CG17800-PAO (Dscam), mRNA 
0.2   1  2  2  5  NM_001043053.1  CG17800-PAG (Dscam), mRNA 
0.2   1  2  2  5  NM_001043058.1  CG17800-RJ, transcript variant J (Dscam), mRNA 
0.2   1  2  2  5  NM_001043062.1  CG17800-PAF (Dscam), mRNA 
0.2   1  2  2  5  NM_001043028.1  CG17800-RR, transcript variant R (Dscam), mRNA 
0.2   1  2  2  4  NM_001043061.1  CG17800-PAD (Dscam), mRNA 
0.2   1  2  2  4  NM_001043012.1  CG17800-PAC (Dscam), mRNA 
0.2   1  2  2  4  NM_001043050.1  CG17800-PAB (Dscam), mRNA 
0.2   1  2  2  4  NM_001043036.1  CG17800-PAA (Dscam), mRNA 
0.2   1  2  2  4  NM_001043016.1  CG17800-PBG (Dscam), mRNA 
0.2   1  2  2  4  NM_001043052.1  CG17800-RZ, transcript variant Z (Dscam), mRNA 
0.2   1  2  2  4  NM_001043026.1  CG17800-RY, transcript variant Y (Dscam), mRNA 
0.2   1  2  2  4  NM_001043056.1  CG17800-RW, transcript variant W (Dscam), mRNA 
0.2   1  2  2  4  NM_001043045.1  CG17800-PAK (Dscam), mRNA 
0.2   1  2  2  4  NM_001043018.1  CG17800-RV, transcript variant V (Dscam), mRNA 
0.2   1  2  2  4  NM_001043046.1  CG17800-RU, transcript variant U (Dscam), mRNA 
0.2   1  2  2  4  NM_001043035.1  CG17800-RT, transcript variant T (Dscam), mRNA 
0.2   1  2  2  4  NM_001043054.1  CG17800-RS, transcript variant S (Dscam), mRNA 
0.2   1  2  2  4  NM_001043042.1  CG17800-RQ, transcript variant Q (Dscam), mRNA 
0.2   1  2  2  4  NM_001043014.1  CG17800-PAL (Dscam), mRNA 

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