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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5370R-2 |
| Symbol |
Dcp-1 |
Full Name |
Death caspase-1 |
| CG No |
CG5370 |
Old CG No |
CG5370 |
| Synonyms |
Dcp-1, dcp-1, DCP-1, Dcp1, dcp1, DCP1, l(2)02132, l(2)01862, CG5370 |
| Accession No (Link to NCBI) |
NM_057626.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Li M, Sun S, Priest J, Bi X, Fan Y.
Characterization of TNF-induced cell death in Drosophila reveals caspase- and JNK-dependent necrosis and its role in tumor suppression.
Cell Death Dis (2019) 10(8) 613
[
PubMed ID = 31409797
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Zhang S, Chen C, Wu C, Yang Y, Li W, Xue L.
The canonical Wg signaling modulates Bsk-mediated cell death in Drosophila.
Cell Death Dis (2015) 6(4) e1713
[
PubMed ID = 25855961
]
[
RRC reference
]
Leulier F, Ribeiro PS, Palmer E, Tenev T, Takahashi K, Robertson D, Zachariou A, Pichaud F, Ueda R, Meier P.
Systematic in vivo RNAi analysis of putative components of the Drosophila cell death machinery.
Cell Death Differ (2006) 13(10) 1663-74
[
PubMed ID = 16485033
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGACGAGTGCGTAACCAGAAAC |
| Primer Seq. 3' |
aatctagaggtaccAGCTTGTACTGCGTATCCTTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GACGAGTGCG TAACCAGAAA CTACGGAGtN NGCATTCGCA GCCCAAATGG ATCGGAAAAT
0061 CGGGGCAGCT TTATAATGGC TGATAATACC GATGCCAAgG GNTGTACGCC GGAGTCTCTT
0121 GTTGTTGGTG GTGCAACTGC AGCGAGCCCC TTGCCNGNCA ACAAGTTTGT GNCTCNAATG
0181 NCCGTgGAAN GATATGCAAG CGAATACAAC ATGAGTCACA ANGCACCGCG GAGTTGCGCT
0241 GATNCTTCAA CCACGAATTC TTCGACATAC CCTCGCTGAA GAGCCGCACC GGAACAAATG
0301 TCGATGCCCA AGAGCTGAAA AAGGCCTTTG AAAACCTGGG ATTCGCGGTG TCCGTGCACA
0361 AGGACTGCAA GTTGAGGGAC ATCCTGAAGC ACGTGGGGAA GGCCGCCGAG TTGGACCACA
0421 CGGACAACGA CTGcCTTGCG GTGGCCATAC TCTCGCACGG GGAGCACGGC TACCTGTACG
0481 CCAAGGATAC GCAGTACAAG CT |
| in silico PCR Fragment |
0001 GACGAGTGCG TAACCAGAAA CTACGGAGTA GGCATTCGCA GCCCAAATGG ATCGGAAAAT
0061 CGGGGCAGCT TTATAATGGC TGATAATACC GATGCCAAGG GCTGTACGCC GGAGTCTCTT
0121 GTTGTTGGTG GTGCAACTGC AGCGAGCCCC TTGCCGGCCA ACAAGTTTGT GGCTCGAATG
0181 CCCGTGGAAC GATATGCAAG CGAATACAAC ATGAGTCACA A-GCACCGCG GAGTTGCGCT
0241 GAT-CTTCAA CCACGAATTC TTCGACATAC CCTCGCTGAA GAGCCGCACC GGAACAAATG
0301 TCGATGCCCA AGAGCTGAAA AAGGCCTTTG AAAACCTGGG ATTCGCGGTG TCCGTGCACA
0361 AGGACTGCAA GTTGAGGGAC ATCCTGAAGC ACGTGGGGAA GGCCGCCGAG TTGGACCACA
0421 CGGACAACGA CTGCCTTGCG GTGGCCATAC TCTCGCACGG GGAGCACGGC TACCTGTACG
0481 CCAAGGATAC GCAGTACAAG CT |
| Assemble Data |
5370R-2.IR_full 1 GACGAGTGCGTAACCAGAAACTACGGAGTNNGCATTCGCAGCCCAAATGGATCGGAAAAT 60
||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
5370R-2.in silico 1 GACGAGTGCGTAACCAGAAACTACGGAGTAGGCATTCGCAGCCCAAATGGATCGGAAAAT 60
5370R-2.IR_full 61 CGGGGCAGCTTTATAATGGCTGATAATACCGATGCCAAGGGNTGTACGCCGGAGTCTCTT 120
||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
5370R-2.in silico 61 CGGGGCAGCTTTATAATGGCTGATAATACCGATGCCAAGGGCTGTACGCCGGAGTCTCTT 120
5370R-2.IR_full 121 GTTGTTGGTGGTGCAACTGCAGCGAGCCCCTTGCCNGNCAACAAGTTTGTGNCTCNAATG 180
||||||||||||||||||||||||||||||||||| | ||||||||||||| ||| ||||
5370R-2.in silico 121 GTTGTTGGTGGTGCAACTGCAGCGAGCCCCTTGCCGGCCAACAAGTTTGTGGCTCGAATG 180
5370R-2.IR_full 181 CCGTGGAANGATATGCAAGCGAATACAACATGAGTCACAANGCACCGCGGAGTTGCGCT 239
|||||||| ||||||||||||||||||||||||||||||| ||||||||||||||||||
5370R-2.in silico 181 CCGTGGAACGATATGCAAGCGAATACAACATGAGTCACAA-GCACCGCGGAGTTGCGCT 239
5370R-2.IR_full 241 GATNCTTCAACCACGAATTCTTCGACATACCCTCGCTGAAGAGCCGCACCGGAACAAATG 299
||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5370R-2.in silico 241 GAT-CTTCAACCACGAATTCTTCGACATACCCTCGCTGAAGAGCCGCACCGGAACAAATG 299
5370R-2.IR_full 301 TCGATGCCCAAGAGCTGAAAAAGGCCTTTGAAAACCTGGGATTCGCGGTGTCCGTGCACA 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5370R-2.in silico 301 TCGATGCCCAAGAGCTGAAAAAGGCCTTTGAAAACCTGGGATTCGCGGTGTCCGTGCACA 359
5370R-2.IR_full 361 AGGACTGCAAGTTGAGGGACATCCTGAAGCACGTGGGGAAGGCCGCCGAGTTGGACCACA 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5370R-2.in silico 361 AGGACTGCAAGTTGAGGGACATCCTGAAGCACGTGGGGAAGGCCGCCGAGTTGGACCACA 419
5370R-2.IR_full 421 CGGACAACGACTGCCTTGCGGTGGCCATACTCTCGCACGGGGAGCACGGCTACCTGTACG 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5370R-2.in silico 421 CGGACAACGACTGCCTTGCGGTGGCCATACTCTCGCACGGGGAGCACGGCTACCTGTACG 479
5370R-2.IR_full 481 CCAAGGATACGCAGTACAAGCT 501
||||||||||||||||||||||
5370R-2.in silico 481 CCAAGGATACGCAGTACAAGCT 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_057626.3 |
CG5370-RA (Dcp-1), mRNA |
| 0.2 |
1 |
2 |
3 |
3 |
NM_137155.2 |
CG10228-RA (Pcf11), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_142844.1 |
CG7031-RA (CG7031), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_132005.1 |
CG15780-RA (CG15780), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_140729.1 |
CG13733-RA (CG13733), mRNA |
| 0 |
0 |
1 |
19 |
47 |
NM_079827.2 |
CG7788-RA (Ice), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_141113.2 |
CG7442-RA (CG7442), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_167803.1 |
CG18374-RB, transcript variant B (Gyk), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_079916.2 |
CG18374-RA, transcript variant A (Gyk), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_136485.2 |
CG30377-RA (CG30377), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_143973.2 |
CG17278-RA (CG17278), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_001014526.1 |
CG33528-RE, transcript variant E (CG33528), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_001014525.1 |
CG33528-RD, transcript variant D (CG33528), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_001014524.1 |
CG33528-RC, transcript variant C (CG33528), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_132588.1 |
CG15728-RA (CG15728), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169358.1 |
CG14685-RA, transcript variant A (Cap-H2), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_141757.1 |
CG14685-RB, transcript variant B (Cap-H2), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001032022.1 |
CG11779-RD, transcript variant D (CG11779), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001032023.1 |
CG11779-RC, transcript variant C (CG11779), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169849.1 |
CG11779-RB, transcript variant B (CG11779), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_142529.3 |
CG11779-RA, transcript variant A (CG11779), mRNA |
| 0 |
0 |
0 |
10 |
13 |
NM_138229.1 |
CG13908-RA (CG13908), mRNA |
| 0 |
0 |
0 |
6 |
10 |
NM_143765.2 |
CG12218-RA (mei-P26), mRNA |
| 0 |
0 |
0 |
6 |
10 |
NM_142053.1 |
CG14366-RA (CG14366), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_141590.2 |
CG11964-RA (CG11964), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_141665.2 |
CG8348-RA (Dh), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_080081.2 |
CG11450-RA (net), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_167274.1 |
CG1938-RD, transcript variant D (Dlic2), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_132466.2 |
CG1938-RA, transcript variant A (Dlic2), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_167273.1 |
CG1938-RC, transcript variant C (Dlic2), mRNA |
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