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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5362R-3 |
| Symbol |
CG5362 |
Full Name |
CG5362 |
| CG No |
CG5362 |
Old CG No |
CG5362 |
| Synonyms |
CT17038, CG5362 |
| Accession No (Link to NCBI) |
NM_135550.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTCGTGTTGTGGTGACCGGAG |
| Primer Seq. 3' |
aatctagaggtaccCAATTTGGCGGCGATCTGGGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TTCGTGTTGT GGTGACCGGA GCCGCTGGCC AAATCGCCTA CTCCCTGCTG TACATGATTG
0061 CTCCGCGGCG AGGTGTTCGG CAAGGATCAG CCCATCGTGC TGCACCTGCT CGACATTCCG
0121 CCCATGGTTG GTGTGCTCGA GGGCGTGGTC ATGGAATTGG CCGACTGCGC TCTGCCGCTG
0181 CTGGTCGAGG TGGTGCCCAC CACCGACCCG GCTGTTGGAT TCAAGGATGT CTCGGCCGCT
0241 TTCCTCGTGG GCGCCATGCC CCGCAAGGAG GGAATGGAGC GAAAGGATCT GCTGTCCGCC
0301 AACGTGAAGA TCTTCAGGAC CCAGGGCCAG GCTCTGGACA AGTTCGCCAA GAAGGACGTC
0361 AAGGTGCTGG TTGTGGGCAA CCCGGCCAAT ACCAATGCCC TGGTCTGCTC CTCCTATGCG
0421 CCTTCCATTC CGCGCGAAAA TTTCTCGGCT ATGACTCGCC TGGATCAGAA TCGCGCCACA
0481 TCCCAGATCG CCGCCAAATT G |
| in silico PCR Fragment |
0001 TTCGTGTTGT GGTGACCGGA GCCGCTGGCC AAATCGCCTA CTCCCTGCTG TACATGATTG
0061 C-CCGCGGCG AGGTGTTCGG CAAGGATCAG CCCATCGTGC TGCACCTGCT CGACATTCCG
0121 CCCATGGTTG GTGTGCTCGA GGGCGTGGTC ATGGAATTGG CCGACTGCGC TCTGCCGCTG
0181 CTGGTCGAGG TGGTGCCCAC CACCGACCCG GCTGTTGGAT TCAAGGATGT CTCGGCCGCT
0241 TTCCTCGTGG GCGCCATGCC CCGCAAGGAG GGAATGGAGC GAAAGGATCT GCTGTCCGCC
0301 AACGTGAAGA TCTTCAGGAC CCAGGGCCAG GCTCTGGACA AGTTCGCCAA GAAGGACGTC
0361 AAGGTGCTGG TTGTGGGCAA CCCGGCCAAT ACCAATGCCC TGGTCTGCTC CTCCTATGCG
0421 CCTTCCATTC CGCGCGAAAA TTTCTCGGCT ATGACTCGCC TGGATCAGAA TCGCGCCACA
0481 TCCCAGATCG CCGCCAAATT G |
| Assemble Data |
5362R-3.IR_full 1 TTCGTGTTGTGGTGACCGGAGCCGCTGGCCAAATCGCCTACTCCCTGCTGTACATGATTG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5362R-3.in silico 1 TTCGTGTTGTGGTGACCGGAGCCGCTGGCCAAATCGCCTACTCCCTGCTGTACATGATTG 60
5362R-3.IR_full 61 CTCCGCGGCGAGGTGTTCGGCAAGGATCAGCCCATCGTGCTGCACCTGCTCGACATTCCG 120
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5362R-3.in silico 61 C-CCGCGGCGAGGTGTTCGGCAAGGATCAGCCCATCGTGCTGCACCTGCTCGACATTCCG 120
5362R-3.IR_full 121 CCCATGGTTGGTGTGCTCGAGGGCGTGGTCATGGAATTGGCCGACTGCGCTCTGCCGCTG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5362R-3.in silico 121 CCCATGGTTGGTGTGCTCGAGGGCGTGGTCATGGAATTGGCCGACTGCGCTCTGCCGCTG 180
5362R-3.IR_full 181 CTGGTCGAGGTGGTGCCCACCACCGACCCGGCTGTTGGATTCAAGGATGTCTCGGCCGCT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5362R-3.in silico 181 CTGGTCGAGGTGGTGCCCACCACCGACCCGGCTGTTGGATTCAAGGATGTCTCGGCCGCT 240
5362R-3.IR_full 241 TTCCTCGTGGGCGCCATGCCCCGCAAGGAGGGAATGGAGCGAAAGGATCTGCTGTCCGCC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5362R-3.in silico 241 TTCCTCGTGGGCGCCATGCCCCGCAAGGAGGGAATGGAGCGAAAGGATCTGCTGTCCGCC 300
5362R-3.IR_full 301 AACGTGAAGATCTTCAGGACCCAGGGCCAGGCTCTGGACAAGTTCGCCAAGAAGGACGTC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5362R-3.in silico 301 AACGTGAAGATCTTCAGGACCCAGGGCCAGGCTCTGGACAAGTTCGCCAAGAAGGACGTC 360
5362R-3.IR_full 361 AAGGTGCTGGTTGTGGGCAACCCGGCCAATACCAATGCCCTGGTCTGCTCCTCCTATGCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5362R-3.in silico 361 AAGGTGCTGGTTGTGGGCAACCCGGCCAATACCAATGCCCTGGTCTGCTCCTCCTATGCG 420
5362R-3.IR_full 421 CCTTCCATTCCGCGCGAAAATTTCTCGGCTATGACTCGCCTGGATCAGAATCGCGCCACA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5362R-3.in silico 421 CCTTCCATTCCGCGCGAAAATTTCTCGGCTATGACTCGCCTGGATCAGAATCGCGCCACA 480
5362R-3.IR_full 481 TCCCAGATCGCCGCCAAATTG 501
|||||||||||||||||||||
5362R-3.in silico 481 TCCCAGATCGCCGCCAAATTG 501
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_135550.1 |
CG5362-RA (CG5362), mRNA |
| 0 |
0 |
5 |
6 |
3 |
NM_168643.2 |
CG13076-RA, transcript variant A (Notum), mRNA |
| 0 |
0 |
5 |
6 |
3 |
NM_168642.2 |
CG13076-RB, transcript variant B (Notum), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_001043260.1 |
CG34157-RD, transcript variant D (Dys), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_141795.2 |
CG6693-RA (CG6693), mRNA |
| 0 |
0 |
3 |
2 |
8 |
NM_079002.2 |
CG3905-RA (Su(z)2), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_135651.2 |
CG4751-RA (CG4751), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_137621.2 |
CG10444-RA (CG10444), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_139636.2 |
CG15015-RA (Cip4), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_078797.2 |
CG13109-RA (tai), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_132412.1 |
CG9817-RA (CG9817), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_168025.1 |
CG12013-RD, transcript variant D (PHGPx), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_168026.1 |
CG12013-RA, transcript variant A (PHGPx), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_139550.2 |
CG12013-RB, transcript variant B (PHGPx), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_168024.1 |
CG12013-RC, transcript variant C (PHGPx), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_142326.2 |
CG16941-RA (CG16941), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_078582.3 |
CG32659-RA (Ten-a), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_080200.2 |
CG5818-RA (mRpL4), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_164617.1 |
CG4145-RC, transcript variant C (Cg25C), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_164616.1 |
CG4145-RB, transcript variant B (Cg25C), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_164615.1 |
CG4145-RA, transcript variant A (Cg25C), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_079470.2 |
CG10564-RA (Ac78C), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_138243.2 |
CG9128-RA (Sac1), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_142561.1 |
CG16727-RA (CG16727), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_168703.2 |
CG11661-RC, transcript variant C (Nc73EF), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_176340.1 |
CG11661-RF, transcript variant F (Nc73EF), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_176341.1 |
CG11661-RG, transcript variant G (Nc73EF), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_168702.1 |
CG11661-RB, transcript variant B (Nc73EF), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_168701.2 |
CG11661-RA, transcript variant A (Nc73EF), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_168704.2 |
CG11661-RE, transcript variant E (Nc73EF), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| + |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| |
|
| VB |
|
| |
|
|
|
|
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