NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 5326R-3

Symbol CG5326 Full Name CG5326

CG No CG5326 Old CG No CG5326

Synonyms CG5326

Accession No (Link to NCBI) NM_142803.1

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAAGCCCTTCGAGCTGAAGAAC

Primer Seq. 3'

aatctagaggtaccTGGATTTGCAGTGTGTGCACG

Predicted Fragment Size

IR fragment full Seq

0001 AAGCCCTTCG AGCTGAAGAA CACTCTCCTG GTCTACAATN GCGGTCCAAG TGCTCCTCAG 
0061 CTGGGTGCTC TTCTACGAGG GATACAAGGG CGGCTGGGGT GGCCACTACA ACTTCAAGTG 
0121 CCAGCCGGTG ACTTACGAAT CGGATCCGAT TTCCATGAGG ATGGCTCGTG CCGTGTGGCT 
0181 GTACTACATT GCCAAGATCA CGGAGCTGTT GGACACCGTG TTCTTTGTGC TGCGCAAGAA 
0241 ACAGCGCCAG ATCTCCTTCC TCCACTTGTA CCACCACACT CTGATGCCCG TGTGTGCCTT 
0301 CATTGGGGTC AAGTACTTTG CCGGTGGCCA TGGAACCCTT CTGGGATTCA TTAACTCCTT 
0361 CATCCACATC ATTATGTACG CCTACTACCT GCTCTCCGCG ATGGGACCCA AGGTGCAAAA 
0421 GTATCTGTGG TGGAAGAACG TACATCACCA TCCTCGCAGA TTGTCCAATT CCTGATCATC 
0481 TTCGTGCACA CACTGCAAAT CCA

in silico PCR Fragment

0001 AAGCCCTTCG AGCTGAAGAA CACTCTCCTG GTCTACAAT- GCGGTCCAAG TGCTCCTCAG 
0061 CTGGGTGCTC TTCTACGAGG GATACAAGGG CGGCTGGGGT GGCCACTACA ACTTCAAGTG 
0121 CCAGCCGGTG ACTTACGAAT CGGATCCGAT TTCCATGAGG ATGGCTCGTG CCGTGTGGCT 
0181 GTACTACATT GCCAAGATCA CGGAGCTGTT GGACACCGTG TTCTTTGTGC TGCGCAAGAA 
0241 ACAGCGCCAG ATCTCCTTCC TCCACTTGTA CCACCACACT CTGATGCCCG TGTGTGCCTT 
0301 CATTGGGGTC AAGTACTTTG CCGGTGGCCA TGGAACCCTT CTGGGATTCA TTAACTCCTT 
0361 CATCCACATC ATTATGTACG CCTACTACCT GCTCTCCGCG ATGGGACCCA AGGTGCAAAA 
0421 GTATCTGTGG TGGAAGAA-G TACATCACCA TCCT-GCAGA TTGTCCAATT CCTGATCATC 
0481 TTCGTGCACA CACTGCAAAT CCA

Assemble Data


5326R-3.IR_full       1   AAGCCCTTCGAGCTGAAGAACACTCTCCTGGTCTACAATNGCGGTCCAAGTGCTCCTCAG 60
                          ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
5326R-3.in silico     1   AAGCCCTTCGAGCTGAAGAACACTCTCCTGGTCTACAAT-GCGGTCCAAGTGCTCCTCAG 60


5326R-3.IR_full       61  CTGGGTGCTCTTCTACGAGGGATACAAGGGCGGCTGGGGTGGCCACTACAACTTCAAGTG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5326R-3.in silico     61  CTGGGTGCTCTTCTACGAGGGATACAAGGGCGGCTGGGGTGGCCACTACAACTTCAAGTG 120


5326R-3.IR_full       121 CCAGCCGGTGACTTACGAATCGGATCCGATTTCCATGAGGATGGCTCGTGCCGTGTGGCT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5326R-3.in silico     121 CCAGCCGGTGACTTACGAATCGGATCCGATTTCCATGAGGATGGCTCGTGCCGTGTGGCT 180


5326R-3.IR_full       181 GTACTACATTGCCAAGATCACGGAGCTGTTGGACACCGTGTTCTTTGTGCTGCGCAAGAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5326R-3.in silico     181 GTACTACATTGCCAAGATCACGGAGCTGTTGGACACCGTGTTCTTTGTGCTGCGCAAGAA 240


5326R-3.IR_full       241 ACAGCGCCAGATCTCCTTCCTCCACTTGTACCACCACACTCTGATGCCCGTGTGTGCCTT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5326R-3.in silico     241 ACAGCGCCAGATCTCCTTCCTCCACTTGTACCACCACACTCTGATGCCCGTGTGTGCCTT 300


5326R-3.IR_full       301 CATTGGGGTCAAGTACTTTGCCGGTGGCCATGGAACCCTTCTGGGATTCATTAACTCCTT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5326R-3.in silico     301 CATTGGGGTCAAGTACTTTGCCGGTGGCCATGGAACCCTTCTGGGATTCATTAACTCCTT 360


5326R-3.IR_full       361 CATCCACATCATTATGTACGCCTACTACCTGCTCTCCGCGATGGGACCCAAGGTGCAAAA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5326R-3.in silico     361 CATCCACATCATTATGTACGCCTACTACCTGCTCTCCGCGATGGGACCCAAGGTGCAAAA 420


5326R-3.IR_full       421 GTATCTGTGGTGGAAGAACGTACATCACCATCCTCGCAGATTGTCCAATTCCTGATCATC 480
                          |||||||||||||||||| ||||||||||||||| |||||||||||||||||||||||||
5326R-3.in silico     421 GTATCTGTGGTGGAAGAA-GTACATCACCATCCT-GCAGATTGTCCAATTCCTGATCATC 480


5326R-3.IR_full       481 TTCGTGCACACACTGCAAATCCA 503
                          |||||||||||||||||||||||
5326R-3.in silico     481 TTCGTGCACACACTGCAAATCCA 503

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_142803.1	CG5326-RA, transcript variant A (CG5326), mRNA
29.25	141	2	1	1	NM_170018.1	CG5326-RB, transcript variant B (CG5326), mRNA
3.11	15	18	16	61	NM_142806.1	CG5278-RA (CG5278), mRNA
1.86	9	6	17	27	NM_141698.1	CG8534-RA (CG8534), mRNA
1.45	7	7	9	25	NM_169312.1	CG9458-RA (CG9458), mRNA
1.03	5	13	10	21	NM_142805.3	CG6921-RB, transcript variant B (CG6921), mRNA
1.03	5	13	10	21	NM_170022.1	CG6921-RA, transcript variant A (CG6921), mRNA
1.03	5	13	10	21	NM_170023.1	CG6921-RC, transcript variant C (CG6921), mRNA
1.03	5	8	6	16	NM_176539.1	CG33110-RA (CG33110), mRNA
0.41	2	15	22	19	NM_141700.1	CG16904-RA (CG16904), mRNA
0	0	19	31	27	NM_141497.1	CG2781-RA (CG2781), mRNA
0	0	6	11	15	NM_137521.1	CG18609-RA (CG18609), mRNA
0	0	5	17	21	NM_141699.1	CG16905-RA (CG16905), mRNA
0	0	4	10	4	NM_165718.1	CG30008-RA (CG30008), mRNA
0	0	3	5	6	NM_139694.2	CG4603-RA (CG4603), mRNA
0	0	2	7	19	NM_141701.2	CG9459-RA (CG9459), mRNA
0	0	2	3	4	NM_143790.1	CG11538-RA (CG11538), mRNA
0	0	2	3	4	NM_080077.2	CG6806-RA (Lsp2), mRNA
0	0	2	3	4	NM_139908.2	CG8042-RA, transcript variant A (CG8042), mRNA
0	0	2	2	4	NM_136059.2	CG10369-RA (Irk3), mRNA
0	0	2	2	2	NM_143240.2	CG6425-RA (CG6425), mRNA
0	0	2	2	2	NM_143560.1	CG15541-RA (CG15541), mRNA
0	0	1	4	2	NM_132479.2	CG1597-RA (CG1597), mRNA
0	0	1	3	5	NM_142957.1	CG13605-RA (CG13605), mRNA
0	0	1	3	4	NM_080138.2	CG1618-RA (comt), mRNA
0	0	1	3	4	NM_132287.2	CG4040-RA (CG4040), mRNA
0	0	1	3	3	NM_079792.2	CG5441-RA (dei), mRNA
0	0	1	3	3	NM_176033.1	CG18146-RB, transcript variant B (CG18146), mRNA
0	0	1	3	3	NM_135851.2	CG18146-RA, transcript variant A (CG18146), mRNA
0	0	1	2	6	NM_139725.2	CG5249-RA (Blimp-1), mRNA

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