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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 5317R-2 
 Symbol CG5317  Full Name CG5317 
 CG No CG5317  Old CG No CG5317 
 Synonyms CG5317 
 Accession No (Link to NCBI) NM_135699.2 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGTCAGACAAATATGCGGTGC 
 Primer Seq. 3' 
aatctagaggtaccGAAGCCCTTCTTGAAGACCAG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TGTCAGACAA ATATGCGGTG CCCAACAAAT TGCCCGGCAA AACAGTGTCG GTGCTAAAGC 
0061 ATCGCAAGAG GAGAATTCTG CAGGATGAAT CTGTTGTTAC GCAGAAAAGA AATGACCGCA 
0121 TCAAAAAGAG AACCGCCACC AAGAATCACC ACAAATTCCG CCGTGCCGAA TCCTTCGTAA 
0181 TGGGCTACCT GAAANGCAGA GCGCACGTCC AAGCGGATCA AGCAAACCAT TCTGCGCACC 
0241 AATGTCACCG AACAAAGTGC CAAAGCTGCC GACGATTCCA ATCCCAAACT GCTCTTCGTG 
0301 ATGCGCCATG CGGGCAAGAA GATCTTCGAC AAGACCACCG CGGATATATT CCGAACCCTG 
0361 CNGCANGGgc TCGCGCCACA ACGCCGTCTT CATGGAGAAC ACCAAGGAGA ACCAGTTGCt 
0421 gNTGCGCGTT ATCGAACCCT TTGTAGTCTA CGGCAATCCC TcGCTGAGCT CCATTCGCGA 
0481 GCTGGTCTTC AAGAAGGGCT TC 
 in silico PCR Fragment 
0001 TGTCAGACAA ATATGCGGTG CCCAACAAAT TGCCCGGCAA AACAGTGTCG GTGCTAAAGC 
0061 ATCGCAAGAG GAGAATTCTG CAGGATGAAT CTGTTGTTAC GCAGAAAAGA AATGACCGCA 
0121 TCAAAAAGAG AACCGCCACC AAGAATCACC ACAAATTCCG CCGTGCCGAA TCCTTCGTAA 
0181 TGGGCTACCT GAAA-GCAGA GCGCACGTCC AAGCGGATCA AGCAAACCAT TCTGCGCACC 
0241 AATGTCACCG AACAAAGTGC CAAAGCTGCC GACGATTCCA ATCCCAAACT GCTCTTCGTG 
0301 ATGCGCCATG CGGGCAAGAA GATCTTCGAC AAGACCACCG CGGATATATT CCGAACCCTG 
0361 C-GCATGGGC TCGCGCCACA ACGCCGTCTT CATGGAGAAC ACCAAGGAGA ACCAGTTGCT 
0421 GCTGCGCGTT ATCGAACCCT TTGTAGTCTA CGGCAATCCC TCGCTGAGCT CCATTCGCGA 
0481 GCTGGTCTTC AAGAAGGGCT TC 
 Assemble Data 

5317R-2.IR_full       1   TGTCAGACAAATATGCGGTGCCCAACAAATTGCCCGGCAAAACAGTGTCGGTGCTAAAGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5317R-2.in silico     1   TGTCAGACAAATATGCGGTGCCCAACAAATTGCCCGGCAAAACAGTGTCGGTGCTAAAGC 60


5317R-2.IR_full       61  ATCGCAAGAGGAGAATTCTGCAGGATGAATCTGTTGTTACGCAGAAAAGAAATGACCGCA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5317R-2.in silico     61  ATCGCAAGAGGAGAATTCTGCAGGATGAATCTGTTGTTACGCAGAAAAGAAATGACCGCA 120


5317R-2.IR_full       121 TCAAAAAGAGAACCGCCACCAAGAATCACCACAAATTCCGCCGTGCCGAATCCTTCGTAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5317R-2.in silico     121 TCAAAAAGAGAACCGCCACCAAGAATCACCACAAATTCCGCCGTGCCGAATCCTTCGTAA 180


5317R-2.IR_full       181 TGGGCTACCTGAAANGCAGAGCGCACGTCCAAGCGGATCAAGCAAACCATTCTGCGCACC 240
                          |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
5317R-2.in silico     181 TGGGCTACCTGAAA-GCAGAGCGCACGTCCAAGCGGATCAAGCAAACCATTCTGCGCACC 240


5317R-2.IR_full       241 AATGTCACCGAACAAAGTGCCAAAGCTGCCGACGATTCCAATCCCAAACTGCTCTTCGTG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5317R-2.in silico     241 AATGTCACCGAACAAAGTGCCAAAGCTGCCGACGATTCCAATCCCAAACTGCTCTTCGTG 300


5317R-2.IR_full       301 ATGCGCCATGCGGGCAAGAAGATCTTCGACAAGACCACCGCGGATATATTCCGAACCCTG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5317R-2.in silico     301 ATGCGCCATGCGGGCAAGAAGATCTTCGACAAGACCACCGCGGATATATTCCGAACCCTG 360


5317R-2.IR_full       361 CNGCANGGGCTCGCGCCACAACGCCGTCTTCATGGAGAACACCAAGGAGAACCAGTTGCT 420
                          | ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
5317R-2.in silico     361 C-GCATGGGCTCGCGCCACAACGCCGTCTTCATGGAGAACACCAAGGAGAACCAGTTGCT 420


5317R-2.IR_full       421 GNTGCGCGTTATCGAACCCTTTGTAGTCTACGGCAATCCCTCGCTGAGCTCCATTCGCGA 480
                          | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5317R-2.in silico     421 GCTGCGCGTTATCGAACCCTTTGTAGTCTACGGCAATCCCTCGCTGAGCTCCATTCGCGA 480


5317R-2.IR_full       481 GCTGGTCTTCAAGAAGGGCTTC 502
                          ||||||||||||||||||||||
5317R-2.in silico     481 GCTGGTCTTCAAGAAGGGCTTC 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_135699.2  CG5317-RA (CG5317), mRNA 
0.2   1  2  4  2  NM_079564.3  CG8874-RA, transcript variant A (Fps85D), mRNA 
0.2   1  2  4  2  NM_169274.1  CG8874-RB, transcript variant B (Fps85D), mRNA 
0.2   1  2  3  2  NM_164746.1  CG4889-RB, transcript variant B (wg), mRNA 
0.2   1  2  3  2  NM_078778.3  CG4889-RA, transcript variant A (wg), mRNA 
0   0  4  2  2  NM_142742.2  CG6332-RA (CG6332), mRNA 
0   0  2  3  2  NM_141964.2  CG6225-RA (CG6225), mRNA 
0   0  2  3  2  NM_139547.1  CG12077-RA (CG12077), mRNA 
0   0  2  2  5  NM_138052.2  CG3356-RA (CG3356), mRNA 
0   0  2  2  3  NM_139389.2  CG12011-RA (CG12011), mRNA 
0   0  1  4  2  NM_136104.2  CG10687-RA (Aats-asn), mRNA 
0   0  1  3  4  NM_169557.1  CG9285-RB, transcript variant B (Dip-B), mRNA 
0   0  1  3  4  NM_169558.1  CG9285-RC, transcript variant C (Dip-B), mRNA 
0   0  1  3  4  NM_142061.2  CG9285-RA, transcript variant A (Dip-B), mRNA 
0   0  1  2  5  NM_134961.1  CG10019-RA (CG10019), mRNA 
0   0  1  2  4  NM_132363.1  CG1791-RA (CG1791), mRNA 
0   0  1  2  4  NM_142597.2  CG12254-RA (MED25), mRNA 
0   0  1  2  3  NM_140267.1  CG14126-RA (CG14126), mRNA 
0   0  1  2  2  NM_142191.1  CG6218-RA (CG6218), mRNA 
0   0  1  1  5  NM_132073.1  CG14445-RA (CG14445), mRNA 
0   0  0  6  7  NM_136344.1  CG14470-RA (CG14470), mRNA 
0   0  0  5  8  NM_079766.2  CG7005-RA (Esp), mRNA 
0   0  0  5  6  NM_057981.3  CG4063-RA (ebi), mRNA 
0   0  0  5  5  NM_057949.3  CG10270-RA (D19B), mRNA 
0   0  0  4  5  NM_168384.1  CG6718-RC, transcript variant C (CG6718), mRNA 
0   0  0  4  5  NM_140109.1  CG6718-RA, transcript variant A (CG6718), mRNA 
0   0  0  4  5  NM_168385.1  CG6718-RD, transcript variant D (CG6718), mRNA 
0   0  0  4  5  NM_168383.1  CG6718-RB, transcript variant B (CG6718), mRNA 
0   0  0  4  5  NM_001032073.1  CG6713-RB, transcript variant B (Nos), mRNA 
0   0  0  4  5  NM_001032072.1  CG6713-RC, transcript variant C (Nos), mRNA 

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