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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5315R-4 |
| Symbol |
CG5315 |
Full Name |
CG5315 |
| CG No |
CG5315 |
Old CG No |
CG5315 |
| Synonyms |
CG5315 |
| Accession No (Link to NCBI) |
NM_142804.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Xu C, Franklin B, Tang HW, Regimbald-Dumas Y, Hu Y, Ramos J, Bosch JA, Villalta C, He X, Perrimon N.
An in vivo RNAi screen uncovers the role of AdoR signaling and adenosine deaminase in controlling intestinal stem cell activity.
Proc Natl Acad Sci U S A (2020) 117(1) 464-471
[
PubMed ID = 31852821
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGACGGAAGTGCTGAAGGCC |
| Primer Seq. 3' |
aatctagaggtaccGGCACAAAGGAACCCATGATC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGACGGAAG TGCTGAAGGC CGGAGTTCTC TCCGATGAAA TTGATTTGGG CGCATTGGCA
0061 CACAATGCCG CCGAGCAGGC AGAGGAGTTT GTGCGCAAGG TCTGGGAGGC TAGCTGGAAA
0121 GTGTGCCACT ACAAAAATCT ACCCAAGTGG CTGCAGGATA ACGACTTCCT ACACCGCGGC
0181 CATCGCCCAC CGCTCCCCTC TTTCCGTGCG TGCTTTAAAT CAATTTTCCG CGTGCACACG
0241 GAAACGGGCA ACATCTGGAC ACATCTTCTT GGCTGCATCG CCTTCATCGG CGTGGCATTA
0301 TACTTCATCT CGCGTCCCTC GGTTGAGATC CAGACACAGG AGAAGATCGT TTTTGGCGCC
0361 TTCTTTATCG GTGCGATTGT GTGCCTAGGA TTCTCATTCG CTTTTCACAC ACTGAGCTGT
0421 CATTCTGTAG AGATGGGAAG ACTGTTTTCT AAACTTGACT ACTGTGGAAT TgNGCTGCTG
0481 ATCATGGGTT CCTTTGTNCC |
| in silico PCR Fragment |
0001 ATGACGGAAG TGCTGAAGGC CGGAGTTCTC TCCGATGAAA TTGATTTGGG CGCATTGGCA
0061 CACAATGCCG CCGAGCAGGC AGAGGAGTTT GTGCGCAAGG TCTGGGAGGC TAGCTGGAAA
0121 GTGTGCCACT ACAAAAATCT ACCCAAGTGG CTGCAGGATA ACGACTTCCT ACACCGCGGC
0181 CATCGCCCAC CGCTCCCCTC TTTCCGTGCG TGCTTTAAAT CAATTTTCCG CGTGCACACG
0241 GAAACGGGCA ACATCTGGAC ACATCTTCTT GGCTGCATCG CCTTCATCGG CGTGGCATTA
0301 TACTTCATCT CGCGTCCCTC GGTTGAGATC CAGACACAGG AGAAGATCGT TTTTGGCGCC
0361 TTCTTTATCG GTGCGATTGT GTGCCTAGGA TTCTCATTCG CTTTTCACAC ACTGAGCTGT
0421 CATTCTGTAG AGATGGGAAG ACTGTTTTCT AAACTTGACT ACTGTGGAAT TGCGCTGCTG
0481 ATCATGGGTT CCTTTGTGCC |
| Assemble Data |
5315R-4.IR_full 1 ATGACGGAAGTGCTGAAGGCCGGAGTTCTCTCCGATGAAATTGATTTGGGCGCATTGGCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5315R-4.in silico 1 ATGACGGAAGTGCTGAAGGCCGGAGTTCTCTCCGATGAAATTGATTTGGGCGCATTGGCA 60
5315R-4.IR_full 61 CACAATGCCGCCGAGCAGGCAGAGGAGTTTGTGCGCAAGGTCTGGGAGGCTAGCTGGAAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5315R-4.in silico 61 CACAATGCCGCCGAGCAGGCAGAGGAGTTTGTGCGCAAGGTCTGGGAGGCTAGCTGGAAA 120
5315R-4.IR_full 121 GTGTGCCACTACAAAAATCTACCCAAGTGGCTGCAGGATAACGACTTCCTACACCGCGGC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5315R-4.in silico 121 GTGTGCCACTACAAAAATCTACCCAAGTGGCTGCAGGATAACGACTTCCTACACCGCGGC 180
5315R-4.IR_full 181 CATCGCCCACCGCTCCCCTCTTTCCGTGCGTGCTTTAAATCAATTTTCCGCGTGCACACG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5315R-4.in silico 181 CATCGCCCACCGCTCCCCTCTTTCCGTGCGTGCTTTAAATCAATTTTCCGCGTGCACACG 240
5315R-4.IR_full 241 GAAACGGGCAACATCTGGACACATCTTCTTGGCTGCATCGCCTTCATCGGCGTGGCATTA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5315R-4.in silico 241 GAAACGGGCAACATCTGGACACATCTTCTTGGCTGCATCGCCTTCATCGGCGTGGCATTA 300
5315R-4.IR_full 301 TACTTCATCTCGCGTCCCTCGGTTGAGATCCAGACACAGGAGAAGATCGTTTTTGGCGCC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5315R-4.in silico 301 TACTTCATCTCGCGTCCCTCGGTTGAGATCCAGACACAGGAGAAGATCGTTTTTGGCGCC 360
5315R-4.IR_full 361 TTCTTTATCGGTGCGATTGTGTGCCTAGGATTCTCATTCGCTTTTCACACACTGAGCTGT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5315R-4.in silico 361 TTCTTTATCGGTGCGATTGTGTGCCTAGGATTCTCATTCGCTTTTCACACACTGAGCTGT 420
5315R-4.IR_full 421 CATTCTGTAGAGATGGGAAGACTGTTTTCTAAACTTGACTACTGTGGAATTGNGCTGCTG 480
|||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
5315R-4.in silico 421 CATTCTGTAGAGATGGGAAGACTGTTTTCTAAACTTGACTACTGTGGAATTGCGCTGCTG 480
5315R-4.IR_full 481 ATCATGGGTTCCTTTGTNCC 500
||||||||||||||||| ||
5315R-4.in silico 481 ATCATGGGTTCCTTTGTGCC 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
2 |
2 |
NM_142804.1 |
CG5315-RA, transcript variant A (CG5315), mRNA |
| 100 |
482 |
0 |
2 |
2 |
NM_170019.1 |
CG5315-RC, transcript variant C (CG5315), mRNA |
| 100 |
482 |
0 |
2 |
2 |
NM_170020.1 |
CG5315-RB, transcript variant B (CG5315), mRNA |
| 0 |
0 |
6 |
4 |
4 |
NM_134961.1 |
CG10019-RA (CG10019), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_137871.2 |
CG3536-RA (CG3536), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_166551.2 |
CG30267-RA (CG30267), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_143555.2 |
CG9717-RA (CG9717), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_143201.3 |
CG14543-RA (CG14543), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_169745.1 |
CG5175-RB, transcript variant B (kuk), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_142338.1 |
CG5175-RA, transcript variant A (kuk), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_135409.1 |
CG9468-RA (CG9468), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_170430.2 |
CG31037-RA (ca), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_165326.1 |
CG10922-RB, transcript variant B (La), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_057666.3 |
CG10922-RA, transcript variant A (La), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_132362.1 |
CG1986-RA (CG1986), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057784.4 |
CG7833-RA (Orc5), mRNA |
| 0 |
0 |
1 |
1 |
1 |
NM_166065.1 |
CG10149-RA, transcript variant A (Rpn6), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_176348.1 |
CG18135-RD, transcript variant D (CG18135), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_140825.2 |
CG18135-RA, transcript variant A (CG18135), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_176346.1 |
CG18135-RB, transcript variant B (CG18135), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_140759.2 |
CG5546-RA (MED19), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_176347.1 |
CG18135-RC, transcript variant C (CG18135), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_134895.2 |
CG3605-RA (CG3605), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_137287.1 |
CG15712-RA (CG15712), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_142847.1 |
CG6660-RA (CG6660), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_080087.2 |
CG9735-RB, transcript variant B (Aats-trp), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_057424.3 |
CG1913-RA (alphaTub84B), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_169264.1 |
CG9735-RA, transcript variant A (Aats-trp), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_078601.2 |
CG9533-RA (rut), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_136007.2 |
CG7094-RA (CG7094), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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