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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5270R-2 |
| Symbol |
CG5270 |
Full Name |
CG5270 |
| CG No |
CG5270 |
Old CG No |
CG5270 |
| Synonyms |
CG5270 |
| Accession No (Link to NCBI) |
NM_169389.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.
The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in Drosophila.
Front Physiol (2021) 12 690564
[
PubMed ID = 34239451
]
[
RRC reference
]
Fan W, Lam SM, Xin J, Yang X, Liu Z, Liu Y, Wang Y, Shui G, Huang X.
Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition.
PLoS Genet (2017) 13(3) e1006664
[
PubMed ID = 28273089
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCAGCAGGAGGAGAATATGCAG |
| Primer Seq. 3' |
aatctagaggtaccGCCAGCCACAGGACCAAAAGT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CAGCAGGAGG AGAATATGCA GCAGCTGCTA AACTTGTTGC CCAAGGATCA GCGGCAGATC
0061 TTTGAGATCT TTATCCAGTG GCTCAATGGC AAGTCCTCCG GGCCCATTCA ATTGCACCAG
0121 AACGCCCTGC TAACCGTGCT TNCAGGCGTC GCCCTATCCC GCCCTGCAAC TGCTACAGTT
0181 GCTCCACGAA CGGTCCAAAC TGCACATTGG ACAAATAATT TCCACCTTGC TTCGACAGCT
0241 GCTANGATGG CGATGTCGCA TCGCCGCGNT TCCCTTTGTG TGCTGGCCAA TTTTCCGGCA
0301 AGCGTTTTGG AGGCCTCTAC ACTTCGTCAG AAATTGATGA CTCGTCTAGT GGTTGATTCC
0361 ATAACCAGTG AACACTTAAT GCTGACCATG TTGGCGCGCA GCGATGGACA ACTCCTGGAT
0421 GATCTGTTGC ATGAGCAGCA ACTGACCATG CGGGCGCAGT GTAGTGAGGC GGCGCCCACT
0481 AAACTTTTGG TCCTGTGGCT GGC |
| in silico PCR Fragment |
0001 CAGCAGGAGG AGAATATGCA GCAGCTGCTA AACTTGTTGC CCAAGGATCA GCGGCAGATC
0061 TTTGAGATCT TTATCCAGTG GCTCAATGGC AAGTCCTCCG GGCCCATTCA ATTGCACCAG
0121 AACGCCCTGC TAACCGTGCT T-CAGGCGTC GCCCTATCCC GCCCTGCAAC TGCTACAGTT
0181 GCTCCACGAA CGGTCCAAAC TGCACATTGG ACAAATAATT TCCACCTTGC TTCGACAGCT
0241 GCTA-GATGG CGATGTCGCA TCGCCGCG-T TCCCTTTGTG TGCTGGCCAA TTTTCCGGCA
0301 AGCGTTTTGG AGGCCTCTAC ACTTCGTCAG AAATTGATGA CTCGTCTAGT GGTTGATTCC
0361 ATAACCAGTG AACACTTAAT GCTGACCATG TTGGCGCGCA GCGATGGACA ACTCCTGGAT
0421 GATCTGTTGC ATGAGCAGCA ACTGACCATG CGGGCGCAGT GTAGTGAGGC GGCGCCCACT
0481 AAACTTTTGG TCCTGTGGCT GGC |
| Assemble Data |
5270R-2.IR_full 1 CAGCAGGAGGAGAATATGCAGCAGCTGCTAAACTTGTTGCCCAAGGATCAGCGGCAGATC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5270R-2.in silico 1 CAGCAGGAGGAGAATATGCAGCAGCTGCTAAACTTGTTGCCCAAGGATCAGCGGCAGATC 60
5270R-2.IR_full 61 TTTGAGATCTTTATCCAGTGGCTCAATGGCAAGTCCTCCGGGCCCATTCAATTGCACCAG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5270R-2.in silico 61 TTTGAGATCTTTATCCAGTGGCTCAATGGCAAGTCCTCCGGGCCCATTCAATTGCACCAG 120
5270R-2.IR_full 121 AACGCCCTGCTAACCGTGCTTNCAGGCGTCGCCCTATCCCGCCCTGCAACTGCTACAGTT 180
||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
5270R-2.in silico 121 AACGCCCTGCTAACCGTGCTT-CAGGCGTCGCCCTATCCCGCCCTGCAACTGCTACAGTT 180
5270R-2.IR_full 181 GCTCCACGAACGGTCCAAACTGCACATTGGACAAATAATTTCCACCTTGCTTCGACAGCT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5270R-2.in silico 181 GCTCCACGAACGGTCCAAACTGCACATTGGACAAATAATTTCCACCTTGCTTCGACAGCT 240
5270R-2.IR_full 241 GCTANGATGGCGATGTCGCATCGCCGCGNTTCCCTTTGTGTGCTGGCCAATTTTCCGGCA 300
|||| ||||||||||||||||||||||| |||||||||||||||||||||||||||||||
5270R-2.in silico 241 GCTA-GATGGCGATGTCGCATCGCCGCG-TTCCCTTTGTGTGCTGGCCAATTTTCCGGCA 300
5270R-2.IR_full 301 AGCGTTTTGGAGGCCTCTACACTTCGTCAGAAATTGATGACTCGTCTAGTGGTTGATTCC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5270R-2.in silico 301 AGCGTTTTGGAGGCCTCTACACTTCGTCAGAAATTGATGACTCGTCTAGTGGTTGATTCC 360
5270R-2.IR_full 361 ATAACCAGTGAACACTTAATGCTGACCATGTTGGCGCGCAGCGATGGACAACTCCTGGAT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5270R-2.in silico 361 ATAACCAGTGAACACTTAATGCTGACCATGTTGGCGCGCAGCGATGGACAACTCCTGGAT 420
5270R-2.IR_full 421 GATCTGTTGCATGAGCAGCAACTGACCATGCGGGCGCAGTGTAGTGAGGCGGCGCCCACT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5270R-2.in silico 421 GATCTGTTGCATGAGCAGCAACTGACCATGCGGGCGCAGTGTAGTGAGGCGGCGCCCACT 480
5270R-2.IR_full 481 AAACTTTTGGTCCTGTGGCTGGC 503
|||||||||||||||||||||||
5270R-2.in silico 481 AAACTTTTGGTCCTGTGGCTGGC 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_169389.2 |
CG5270-RB, transcript variant B (CG5270), mRNA |
| 0.82 |
4 |
3 |
3 |
3 |
NM_134600.1 |
CG1494-RA (CG1494), mRNA |
| 0 |
0 |
4 |
6 |
4 |
NM_139783.1 |
CG10226-RA (CG10226), mRNA |
| 0 |
0 |
3 |
2 |
1 |
NM_080010.2 |
CG11606-RA (Rpp30), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_170287.1 |
CG5490-RA, transcript variant A (Tl), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_079794.2 |
CG5490-RB, transcript variant B (Tl), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_133156.1 |
CG12202-RA (Nat1), mRNA |
| 0 |
0 |
2 |
2 |
1 |
NM_078846.2 |
CG4182-RA (yellow-c), mRNA |
| 0 |
0 |
2 |
2 |
1 |
NM_080199.2 |
CG4180-RA (l(2)35Bg), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_165156.1 |
CG5813-RB, transcript variant B (chif), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_078859.2 |
CG5813-RA, transcript variant A (chif), mRNA |
| 0 |
0 |
1 |
5 |
9 |
NM_165841.1 |
CG9015-RB, transcript variant B (en), mRNA |
| 0 |
0 |
1 |
5 |
9 |
NM_078976.3 |
CG9015-RA, transcript variant A (en), mRNA |
| 0 |
0 |
1 |
4 |
16 |
NM_079205.3 |
CG7507-RA, transcript variant A (Dhc64C), mRNA |
| 0 |
0 |
1 |
4 |
11 |
NM_168103.1 |
CG7507-RB, transcript variant B (Dhc64C), mRNA |
| 0 |
0 |
1 |
4 |
11 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
1 |
4 |
9 |
NM_132915.1 |
CG15865-RA (CG15865), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_137477.2 |
CG5154-RA (Idgf5), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_001038734.1 |
CG16902-RC (Hr4), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_142524.2 |
CG6040-RA (CG6040), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_001038740.1 |
CG32776-RD, transcript variant D (CG32776), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_206622.2 |
CG32776-RB, transcript variant B (CG32776), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_166996.2 |
CG32776-RA, transcript variant A (CG32776), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_001038741.1 |
CG32776-RC, transcript variant C (CG32776), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166037.1 |
CG30071-RA (CG30071), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_143285.1 |
CG6051-RA (CG6051), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_170158.1 |
CG5794-RB, transcript variant B (CG5794), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_143018.2 |
CG5794-RD, transcript variant D (CG5794), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_080136.1 |
CG18104-RA (arg), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_133116.1 |
CG14190-RA (CG14190), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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