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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5258R-3 |
| Symbol |
NHP2 |
Full Name |
NHP2 |
| CG No |
CG5258 |
Old CG No |
CG5258 |
| Synonyms |
CG5258, FBgn0029148, NHP2 |
| Accession No (Link to NCBI) |
NM_143708.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGGGCAAAGTGAAAGTAGAGC |
| Primer Seq. 3' |
aatctagaggtaccACGGGTATGTTTAGTGCAGAC |
| Predicted Fragment Size |
478 |
| IR fragment full Seq |
0001 TGGGCAAAGT GAAAGTAGAG CGCTCCGAGG ATGCCGATGA GTCCGTTGTG GCCAGCGGCG
0061 ATGTTACCAT CAAGGAGGAG GAGTCCTACG ACGACAAGCT GATCTTTGTG AACGCCATCG
0121 CGAAACCGAT GGCAGGTAAA AAACTGGCCA AAAAGTGCTA CAAACTGGTG AAGAAGGCCA
0181 TGAAGCACAA GACTTTCCTG CGAAATGGAC TTAAGGATGT ACAGACTCGC CTGCGCAAGG
0241 GCGAANACTG GCATTTGCAT CTTTGCTGGC GACGTTACAC CTGTGGACAT CATGTGCCAC
0301 TTACCAGCCG TCTGCGAGGA GAAGGGCATC CCCTATACCT ACACCCCCAG TCGAGCGGAT
0361 CTTGGAGCTG CCATGGGCGT GAAGCGCGGT ACAGTCGCCC TGCTGGTCCG CCAGAACGAG
0421 GAGTACAAGG ATCTGTACGA CGAGGTCAAG GAGGAGCTGT CTGCACTAAA CATACCCGT |
| in silico PCR Fragment |
0001 TGGGCAAAGT GAAAGTAGAG CGCTCCGAGG ATGCCGATGA GTCCGTTGTG GCCAGCGGCG
0061 ATGTTACCAT CAAGGAGGAG GAGTCCTACG ACGACAAGCT GATCTTTGTG AACGCCATCG
0121 CGAAACCGAT GGCAGGTAAA AAACTGGCCA AAAAGTGCTA CAAACTGGTG AAGAAGGCCA
0181 TGAAGCACAA GACTTTCCTG CGAAATGGAC TTAAGGATGT ACAGACTCGC CTGCGCAAGG
0241 GCGAA-ACTG GCATTTGCAT CTTTGCTGGC GACGTTACAC CTGTGGACAT CATGTGCCAC
0301 TTACCAGCCG TCTGCGAGGA GAAGGGCATC CCCTATACCT ACACCCCCAG TCGAGCGGAT
0361 CTTGGAGCTG CCATGGGCGT GAAGCGCGGT ACAGTCGCCC TGCTGGTCCG CCAGAACGAG
0421 GAGTACAAGG ATCTGTACGA CGAGGTCAAG GAGGAGCTGT CTGCACTAAA CATACCCGT |
| Assemble Data |
5258R-3.IR_full 1 TGGGCAAAGTGAAAGTAGAGCGCTCCGAGGATGCCGATGAGTCCGTTGTGGCCAGCGGCG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5258R-3.in silico 1 TGGGCAAAGTGAAAGTAGAGCGCTCCGAGGATGCCGATGAGTCCGTTGTGGCCAGCGGCG 60
5258R-3.IR_full 61 ATGTTACCATCAAGGAGGAGGAGTCCTACGACGACAAGCTGATCTTTGTGAACGCCATCG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5258R-3.in silico 61 ATGTTACCATCAAGGAGGAGGAGTCCTACGACGACAAGCTGATCTTTGTGAACGCCATCG 120
5258R-3.IR_full 121 CGAAACCGATGGCAGGTAAAAAACTGGCCAAAAAGTGCTACAAACTGGTGAAGAAGGCCA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5258R-3.in silico 121 CGAAACCGATGGCAGGTAAAAAACTGGCCAAAAAGTGCTACAAACTGGTGAAGAAGGCCA 180
5258R-3.IR_full 181 TGAAGCACAAGACTTTCCTGCGAAATGGACTTAAGGATGTACAGACTCGCCTGCGCAAGG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5258R-3.in silico 181 TGAAGCACAAGACTTTCCTGCGAAATGGACTTAAGGATGTACAGACTCGCCTGCGCAAGG 240
5258R-3.IR_full 241 GCGAANACTGGCATTTGCATCTTTGCTGGCGACGTTACACCTGTGGACATCATGTGCCAC 300
||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
5258R-3.in silico 241 GCGAA-ACTGGCATTTGCATCTTTGCTGGCGACGTTACACCTGTGGACATCATGTGCCAC 300
5258R-3.IR_full 301 TTACCAGCCGTCTGCGAGGAGAAGGGCATCCCCTATACCTACACCCCCAGTCGAGCGGAT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5258R-3.in silico 301 TTACCAGCCGTCTGCGAGGAGAAGGGCATCCCCTATACCTACACCCCCAGTCGAGCGGAT 360
5258R-3.IR_full 361 CTTGGAGCTGCCATGGGCGTGAAGCGCGGTACAGTCGCCCTGCTGGTCCGCCAGAACGAG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5258R-3.in silico 361 CTTGGAGCTGCCATGGGCGTGAAGCGCGGTACAGTCGCCCTGCTGGTCCGCCAGAACGAG 420
5258R-3.IR_full 421 GAGTACAAGGATCTGTACGACGAGGTCAAGGAGGAGCTGTCTGCACTAAACATACCCGT 479
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5258R-3.in silico 421 GAGTACAAGGATCTGTACGACGAGGTCAAGGAGGAGCTGTCTGCACTAAACATACCCGT 479
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
460 |
0 |
0 |
0 |
NM_143708.2 |
CG5258-RA (NHP2), mRNA |
| 0.65 |
3 |
2 |
2 |
4 |
NM_139424.1 |
CG11814-RA (CG11814), mRNA |
| 0 |
0 |
3 |
4 |
7 |
NM_141151.2 |
CG11370-RA (CG11370), mRNA |
| 0 |
0 |
3 |
4 |
4 |
NM_142190.2 |
CG4931-RA (Sra-1), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_206385.1 |
CG33257-RA (CG33257), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_141279.2 |
CG12147-RA (CG12147), mRNA |
| 0 |
0 |
1 |
7 |
8 |
NM_057328.3 |
CG3127-RA (Pgk), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_141578.1 |
CG8351-RA (CG8351), mRNA |
| 0 |
0 |
1 |
5 |
13 |
NM_078670.2 |
CG7092-RA (Dhc16F), mRNA |
| 0 |
0 |
1 |
5 |
7 |
NM_140532.2 |
CG7372-RA (CG7372), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_166506.1 |
CG13502-RB, transcript variant B (CG13502), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_137793.1 |
CG13502-RA, transcript variant A (CG13502), mRNA |
| 0 |
0 |
1 |
4 |
11 |
NM_165841.1 |
CG9015-RB, transcript variant B (en), mRNA |
| 0 |
0 |
1 |
4 |
11 |
NM_078976.3 |
CG9015-RA, transcript variant A (en), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_141944.1 |
CG5925-RA (desat2), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_137289.1 |
CG7813-RA (CG7813), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_169163.3 |
CG1980-RA, transcript variant A (dj), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_057872.4 |
CG1980-RB, transcript variant B (dj), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_057269.2 |
CG10236-RA (LanA), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_143561.2 |
CG1342-RA (CG1342), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_142773.1 |
CG7069-RA (CG7069), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_137805.1 |
CG11275-RA (CG11275), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_134907.2 |
CG3488-RA (CG3488), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001043153.1 |
CG32434-RC, transcript variant C (siz), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206407.1 |
CG32434-RA, transcript variant A (siz), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_168881.1 |
CG32434-RB, transcript variant B (siz), mRNA |
| 0 |
0 |
1 |
1 |
1 |
NM_169491.1 |
CG7583-RC, transcript variant C (CtBP), mRNA |
| 0 |
0 |
0 |
11 |
2 |
NM_132280.3 |
CG10648-RA (CG10648), mRNA |
| 0 |
0 |
0 |
9 |
6 |
NM_130523.2 |
CG11638-RA (CG11638), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_136846.1 |
CG13198-RA (CG13198), mRNA |
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| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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|
JNK signal
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Caspase-3 signal
| N |
 |
| ++ |
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| DB |
 |
| + |
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| DC |
 |
| ++ |
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| DM |
 |
| + |
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| VM |
 |
| |
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| VB |
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